AFM Temperature control - H TEST a.s.

Agilent Temperature ControlData SheetFigure 1. Patented design Ceramic fixturesinsulate the stage from the sampleplate and are symmetrically designed tocompensate for thermal expansion and/or contraction. The Peltier device quicklyheats or cools the stage to create atemperature gradient across the element.Features and Benefits• Precise temperature control from -30°Cto 250°C with accuracy up to ±0.025°Coffers lowest thermal drift available• Open-access, modular design providesease of use and quicker setup• Simultaneous electrochemical andtemperature control ensures full supportof a broad range of applications• Eliminates condensation withenvironmental control so there is noneed to heat the AFM/SPM tip• Works in air, liquid, and controlledenvironment to afford excellentversatility• Patented design enables high-resolutionimaging at extreme temperatures• Rapid settling time helps yield resultsfaster• Three temperature ranges available tobest meet application needs• Heating: ambient to 250°C• Single Peltier: 0°C to 40°C• Triple Peltier: -30°C to ambientOverviewAgilent’s temperature controlleruses a patented thermal insulationand compensation design to deliverprecise temperature control for highresolutionscanning probe microscopy(SPM). It allows imaging duringtemperature changes and is fullycompatible with all imaging modes.The unique sample plate has built-intemperature control and offersexcellent thermal stability for SPMimaging. The temperature controllerprovides a rapid settling time, therebyallowing the sample plate to reachtemperature quickly and hold constanttemperature for long periods of time.Agilent’s temperature control designisolates the sample plate fromthe rest of the SPM system. Aninsulated ceramic fixture protectsthe surrounding apparatus from theeffects of heating or cooling, thusproviding the most precise, stabletemperature control available for SPM.(Figure 1.)Temperature Drift Rate in X-Y Drift Rate in ZRoom Temp. < 5 Å/min

Application SuitabilityTemperature control plays a criticalrole in SPM research. Temperaturecan be used to modify molecularstructures and accelerate, inhibit, andcontrol the rates of many chemicalreactions. In material sciences,temperature is used to controlphase transition of materials suchas co-polymer and crystal growth.Temperature also plays a veryimportant role in life sciences. Forexample, physiological processescan be accelerated or decelerated,the structures of many biologicalmolecules can be altered, andbiomolecular binding events can becontrolled all by heating or cooling.Applicationsa. Beforec. Afterb. DuringFigure 6. MAC Mode AFM imageof paraffin at 120°C (a) before and(c) after phase transition, and at126°C (b) during phase transition.10μm x 10μm.• Biomolecular studies: decreasetemperature to stabilize or annealmolecules; increase temperatureto induce melting transition ordenature molecules; performexperiments at physiologicaltemperatureScan size 40 µm x 40 µm Scan size 6 µm x 6 µm• Surface chemistry and polymerstudies: control reaction rates;induce phase transitions; inducethermal degradation• Corrosion studies: accelerateoxidation reactions; mimic corrosionconditions• Studies of mechanical or electricaltemperature-dependent propertiesin all applications25° C80° Ca.Figure 7. In situ and temperature-dependentAFM studies of polymer low densitypolyethylene (LDPE). The images on the leftare acquired at 25°C (top), 80°C (middle)and 80°C after heating over 95°C (bottom),respectively. It shows that LDPE canwithstand a temperatures of 80°C for a longtime, but heating above 95°C will lead to astructure transition. The two images on theright are in situ zoom-in demonstrate thestructure change to the annealing.80° C after 95° C3