MBB 601 Advances in Plant Molecular Biology 3+ ... - TNAU Genomics
MBB 601 Advances in Plant Molecular Biology 3+ ... - TNAU Genomics
MBB 601 Advances in Plant Molecular Biology 3+ ... - TNAU Genomics
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Suggested web sites:www.tigr.orgwww.ncbi.nlm.nih.govhttp://genome-www.stanford.eduhttp://signal.salk.edu/cgi-b<strong>in</strong>/RiceGEhttp://atidb.cshl.orghttp://gene64.dna.affrc.go.jp/RPD<strong>MBB</strong> 606 COMMERCIAL PLANT TISSUE CULTURE 2+0ObjectiveTo discuss the commercial applications of plant tissue culture <strong>in</strong> agriculture, horticulture, forestrymedic<strong>in</strong>e and <strong>in</strong>dustryTheoryUNIT I Micro propagation and quality control <strong>in</strong> tissue culture plantsMicro propagation - plant multiplication, harden<strong>in</strong>g - Quality control for tissue culture. Pack<strong>in</strong>g,transportation and quarant<strong>in</strong>e for tissue cultured plants - Low cost alternatives. Bio-reactors -Commercialized photoautotrophic micro propagation-automation technologies-Synthetic seedproduction –applications micro propagation <strong>in</strong> ornamentals, horticultural and plantation crops, treecrops and medic<strong>in</strong>al plants. National certification system for TC plants-consortium of micropropagation research-commercial TC labs <strong>in</strong> India.UNIT II Secondary metabolite production through plant tissue culture and bioreactortechnologySecondary metabolites production - organ, cell suspensions and hairy root cultures - Methods toenhance secondary metabolite production - precursor feed<strong>in</strong>g, elicitation, bio-transformation,immobilization, mutagenesis - Scale up through bioreactors- Secondary metabolites of <strong>in</strong>dustrialand pharmaceutical importance – examples.UNIT III Commercialization of plant tissue culture-patentsProject preparation for commercial plant tissue culture - management and market<strong>in</strong>g of TC plants.Visit to a commercial plant tissue culture laboratory-Recent patent applications <strong>in</strong> tissue culture -product and process patents - <strong>Plant</strong> Stem Cell applications - cosmetic and therapeutic uses.UNIT IV Commercial tissue culture and applications-Commercial tissue culture and applications-case studies-banana, ornamentals, bamboo,sugarcane, tree crops, potato and medic<strong>in</strong>al plantsLecture schedule1. Micro propagation-Methods-stages-<strong>Plant</strong> multiplication, harden<strong>in</strong>g and transplantationadvantagesand problems <strong>in</strong> micro propagation2. Quality control for tissue culture - disease freedom and genetic fidelity3. Pack<strong>in</strong>g, transportation and quarant<strong>in</strong>e for tissue cultured plants4. Low cost alternatives <strong>in</strong> <strong>Plant</strong> tissue culture5. Bioreactors for plant tissue culture-commercialized photoautotrophic micro propagation6. Integrat<strong>in</strong>g automation technologies with commercial micro propagation7. Synthetic seed production-applications11