MBB 601 Advances in Plant Molecular Biology 3+ ... - TNAU Genomics

MBB 601 ADVANCES IN PLANT MOLECULAR BIOLOGY 3+0ObjectiveTo discuss the specialized topics and recent advances in the field of plant molecular biology.TheoryUNIT IArabidopsis in molecular biology, Forward and Reverse Genetic Approaches, Transcriptional andpost-transcriptional regulation of gene expression, isolation of promoters and other regulatoryelements.UNIT IIRNA interference, Transcriptional gene silencing, Transcript and protein analysis, use of transcriptprofiling to study biological systems.UNIT IIIHormone regulatory pathways: Ethylene, Cytokinin, Auxin and ABA, SA and JA; ABC Model ofFloral Development, Molecular basis of self incompatibility. Signal transduction and receptors.UNIT IVMolecular biology of abiotic stress responses: Cold, high temperature, submergence, dehydration,salinity and drought; Molecular Biology of plant-pathogen interactions, molecular biology ofAgrobacterium infection, Molecular biology of Rhizobium infection (molecular mechanisms insymbiosis), Programmed cell death in development and defense.Lecture schedule1. Arabidopsis as a model plant in molecular biology2. The genetic basis of a phenotype or trait3. Expression of a phenotype by a particular gene4. Reverse Genetic Approaches in functional genomics5. Reverse Genetic Approaches in functional genomics (cont…)6. Transcriptional and post-transcriptional regulation of gene expression7. Transcriptional and post-transcriptional regulation of gene expression (cont…)8. Promoters and their role in gene expression9. Isolation of promoters and other regulatory elements10. RNA interference – micro RNAs and small interfering RNAs11. Mode of action in RNAi system12. RNA dependent gene silencing13. Transcriptional gene silencing14. Post transcriptional gene silencing15. RNA viruses in post transcriptional gene silencing16. Suppression of gene silencing by plant viruses17. Application of RNAi technology in plant biotechnology18. Transcript analysis to study biological systems.19. Protein analysis to study biological systems.20. Regulation of cellular processes by different hormones21. Role of hormones in flowering22. Role of hormones in fruit ripening1