MBB 601 Advances in Plant Molecular Biology 3+ ... - TNAU Genomics

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MBB 603 ADVANCES IN MICROBIAL BIOTECHNOLOGY 3+0ObjectiveTo discuss specialized topics about industrially important microorganisms.TheoryUNIT IFermentative metabolism and development of bio-processing technology, processing andproduction of recombinant products; isolation, preservation and improvement of industriallyimportant microorganisms.UNIT IIImmobilization of enzymes and cells; Batch, plug flow and chemo-state-cultures; Computersimulations; Fed-batch and mixed cultures; Scale-up principles; Down stream processing etc.UNIT IIICurrent advances in production of antibiotics, vaccines, and biocides; Steroid transformation;Bioreactors; Bioprocess engineering; Production of non-microbial origin products by geneticallyengineered microorganisms.UNIT IVConcept of probiotics and applications of new tools of biotechnology for quality feed/foodproduction; Microorganisms and proteins used in probiotics; Lactic acid bacteria as live vaccines;Factors affecting delignification; Bioconversion of substrates, anti-nutritional factors present infeeds; Microbial detoxification of aflatoxins; Single cell protein, Bio-insecticides; Bio-fertilizers;Recent advances in microbial biotechnology.Lecture Schedule1. Microbial biotechnology: Scope and techniques.2. Fermentative diversity -Utilization of hexoses by fermentative pathways3. Fermentative diversity-Utilization of pentoses, polysaccharides and organic acid metabolisms4. Fermentation- Microbial Growth Kinetics- applications of Fermentation5. Development of bioprocess technology and production of recombinant products6. Recombinant DNA products for human therapeutic use7. Recombinant DNA products – Challenges and future thrust8. Isolation, screening of industrially important microorganisms9. Selection and Preservation of industrially important microorganisms10. Strain improvement of industrially important microorganisms-11. Strain improvement- the random and empirical approach12. Case studies- strain improvement - Recombinant DNA approaches for low and medium valueproducts13. Case studies- strain improvement-Recombinant DNA approaches- for high value recombinantproducts14. Principles of immobilization- techniques and importance15. Immobilization of enzymes and cells - Current trends and future Prospects16. Case studies- Immobilized cell technology – Flavour maturation of green beer17. Case studies- Immobilized cell technology alcohol free beer18. Case studies- Application of Immobilized Enzymes in pharmaceutical industry19. Bioreactors - types, designs and functional characteristics, computer simulations and Bioprocess5
engineering20. Dual and mixed inoculations - continuous and batch fermentations; plug flow and chemostatcultures21. Fed-batch cultures- Scale up Principles and applications22. Downstream Processing – on overview23. Downstream Processing – Principles and application of RIPP technology24. Case studies on Down stream processing of High value and Low volume Products25. Case studies on Down stream processing of Medium value and Medium volume Products26. Case studies on Down stream processing of Low value and High volume Products27. Mid semester exam28. Production of Secondary metabolites- antibiotics – Problems and Future thrust29. Recombinant and Synthetic Vaccines Problems with Traditional Vaccines30. Impact of Biotechnology on Vaccine Development; Mechanisms for Producing Immunity31. Improving the Effectiveness of Subunit Vaccines; Fragments of Antigen Subunit Used as SyntheticPeptide; DNA Vaccines; Vaccines in Development32. Biocides- Types and Classification33. Standards and Regulation of Biocides application34. Bio-catalysis in organic chemistry - Microbial transformation of Steroids and Sterols35. Production of non-microbial origin products by genetically engineered microorganisms36. Concept of probiotics and applications37. Microorganisms and proteins used in probiotics38. Lactic acid bacteria as live vaccines39. Biotechnological approaches for enhancing quality of feed and food production40. Bioconversion of substrates41. Degradation of Lignin, Cellulose and Hemicelluloses42. Enzymatic Lignocellulose biodegradation43. Prospects of Conversion of biomass – Bio Fuel Ethanol44. Anti-nutritional factors present in feeds45. Biotechnological approaches for reducing the anti- nutritional factors in feed and Food46. Aflotoxins – Current scenario- Microbial detoxification of aflatoxins47. Single cell protein- Types – Present status and Future Thrust48. Advances in Bio-insecticides49. Advances in Bio-fertilizers50. Recent Advances in microbial biotechnology51. Recent Advances in microbial biotechnologySuggested Text Books1. A.N. Glazer and H. Nikaido. (Eds). 2007. Microbial biotechnology: fundamentals of appliedmicrobiology. Cambridge University Press.2. Alagawadi, A.R., P.U. Krishnaraj, K.S. Jagadeesh, J.H. Kulkarni, S. Kannaiyan. 2006.Microbial Biotechnology. Narosa Pub., New Delhi.3. Bryskier, A. (Eds). 2005. Antimicrobial Agents: Antibacterials and Antifungals,Washington,DC: ASM Press4. C.D. Swarna Latha. 2007. Microbial Biotechnology, Discovery Publishing House5. D. Charalampopoulos, R. A. Rastall (Eds.) 2009. Prebiotics and Probiotics Science andTechnology. Springer Publishers.6. Demain, A. L. 2000. Small bugs, big business: the economic power of the microbe.Biotechnology Advances, 18, 499–514.7. El-Mans, E.M.T and C.F.A Bryce. 2002. Fermentation Technology and Biotechnology.6
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