Protein Structures Determined by NMR - ESI

Protein Structures Determined by NMR• Development began in the middle of the 1980s, but most of theearly structures were of small proteins or peptides• You do not determine a single structure as in X-Raycrystallography, but rather an ensemble of structures thatsatisfy both the NMR experimental constraints and generalstructural constraints.• In general, the more NMR experimental restraints that areused, the better the precision of the structures calculated.• Advancements in techniques (Triple resonance, TROSY) andequipment (magnets and computers) have increased the size ofthe molecules that can be studies (proteosome, chaperones) aswell as improved the quality of the structures determined.