Enzymes and Enzyme Kinetics - CMBE

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For convenience define a dimensionless groupDamkohler NumberDa =_____________________Max Rate of ReactionMax Rate of Mass TransferDa =V MAX ′______k L S BULKIf Da >> 1 system is limited by mass transfer“mass transfer limited regime”If Da ~ 1 mass transfer <strong>and</strong> reaction rate are ofsimilar magnitudeIf Da
___________V MAX ′S SURF= k L (S BULK –S SURF )K M + S SURFUnknown: S SURFParameters: S BULK , k L , V MAX ′, K MIntroduce dimensionless parameters:x =______S SURFS BULK =______K MS BULK(kappa) in many books;others define = S BULK /K M
Page 1 and 2: I. Immobilization of Enzymes1. Moti
Page 3 and 4: Disadvantages of Process II• An a
Page 5 and 6: . Now immobilizeSurfacec. Wash the
Page 7 and 8: Case I - Enzyme reaction is very sl
Page 9: CommentsV___________MAX ′S SURF=
Page 13 and 14: So…._______V MAX ′x + x= k L S
Page 15 and 16: . Mass Transfer-Limited Regime_____
Page 17 and 18: Implications of Reaction Rate = k L
Page 19 and 20: c. Reaction-Limited Regime_______x
Page 21 and 22: ___________________________________
Page 23 and 24: Example Problem 3.3 (see pp. 85-86)
Page 25 and 26: B) S BULK = 14 mol/cm 3Da = =V MAX
Page 27 and 28: D) S BULK = 7 mol/cm 3 but k L is 8
Page 29: Summary (units and exponents left o

Enzymes and Enzyme Kinetics - CMBE

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