Enzymes and Enzyme Kinetics - CMBE

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E =__________x ( + 1 )( + x ) E is a measure of the influence ofexternal mass transfer resistance onthe observed reaction rate.if E
Example Problem 3.3 (see pp. 85-86)V MAX ′ = (6 × 10 -6 mol/s·mg)(1 × 10 -4 mg/cm 2 )= 6 × 10 -10 mol/cm 2·s= 6 × 10 -4 mol/cm 2·sK M = 2 × 10 -3 mol/L = 2 mol/cm 3k L = 4.3 × 10 -5 cm/sFind S SURF , V <strong>and</strong> E when …..
Page 1 and 2: I. Immobilization of Enzymes1. Moti
Page 3 and 4: Disadvantages of Process II• An a
Page 5 and 6: . Now immobilizeSurfacec. Wash the
Page 7 and 8: Case I - Enzyme reaction is very sl
Page 9 and 10: CommentsV___________MAX ′S SURF=
Page 11 and 12: ___________V MAX ′S SURF= k L (S
Page 13 and 14: So…._______V MAX ′x + x= k L S
Page 15 and 16: . Mass Transfer-Limited Regime_____
Page 17 and 18: Implications of Reaction Rate = k L
Page 19 and 20: c. Reaction-Limited Regime_______x
Page 21: ___________________________________
Page 25 and 26: B) S BULK = 14 mol/cm 3Da = =V MAX
Page 27 and 28: D) S BULK = 7 mol/cm 3 but k L is 8
Page 29: Summary (units and exponents left o

Enzymes and Enzyme Kinetics - CMBE

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