Enzymes and Enzyme Kinetics - CMBE

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___________V MAX ′S SURF= k L (S BULK –S SURF )K M + S SURFxS BULK = S SURFS BULK = K MThen Reaction Rate =_____________V MAX ′xS BULKS BULK + xS BULK_______V MAX ′x + xThen Mass Transfer Rate =k L (S BULK –xS BULK )k L S BULK (1 – x)
So…._______V MAX ′x + x= k L S BULK (1 – x)Remember….Da =V MAX ′______k L S BULKThus…._______x + x=(1 – x)_______DaWrite as a quadratic….x 2 + Dax + x – x – = 0x 2 + x – = 0Where…. = Da + -1
Page 1 and 2: I. Immobilization of Enzymes1. Moti
Page 3 and 4: Disadvantages of Process II• An a
Page 5 and 6: . Now immobilizeSurfacec. Wash the
Page 7 and 8: Case I - Enzyme reaction is very sl
Page 9 and 10: CommentsV___________MAX ′S SURF=
Page 11: ___________V MAX ′S SURF= k L (S
Page 15 and 16: . Mass Transfer-Limited Regime_____
Page 17 and 18: Implications of Reaction Rate = k L
Page 19 and 20: c. Reaction-Limited Regime_______x
Page 21 and 22: ___________________________________
Page 23 and 24: Example Problem 3.3 (see pp. 85-86)
Page 25 and 26: B) S BULK = 14 mol/cm 3Da = =V MAX
Page 27 and 28: D) S BULK = 7 mol/cm 3 but k L is 8
Page 29: Summary (units and exponents left o

Enzymes and Enzyme Kinetics - CMBE

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