Enzymes and Enzyme Kinetics - CMBE

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How to reduce structural changes <strong>and</strong> preventbinding at activity site:EESurfaceSurfacea. Mix enzyme with competitive inhibitorE+I
. Now immobilizeSurfacec. Wash the competitive inhibitor awaySurface
Page 1 and 2: I. Immobilization of Enzymes1. Moti
Page 3: Disadvantages of Process II• An a
Page 7 and 8: Case I - Enzyme reaction is very sl
Page 9 and 10: CommentsV___________MAX ′S SURF=
Page 11 and 12: ___________V MAX ′S SURF= k L (S
Page 13 and 14: So…._______V MAX ′x + x= k L S
Page 15 and 16: . Mass Transfer-Limited Regime_____
Page 17 and 18: Implications of Reaction Rate = k L
Page 19 and 20: c. Reaction-Limited Regime_______x
Page 21 and 22: ___________________________________
Page 23 and 24: Example Problem 3.3 (see pp. 85-86)
Page 25 and 26: B) S BULK = 14 mol/cm 3Da = =V MAX
Page 27 and 28: D) S BULK = 7 mol/cm 3 but k L is 8
Page 29: Summary (units and exponents left o

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