Disease cycle of potato late blight - MSpace at the University of ...

LIST OF FIGURES
Figure 1.1. Disease cycle of potato late blight …………………………..………….....6
Figure 1.2. Schematic representation of the Phenylpropanoid pathway ……………...15
Figure 1.3. Schematic representation of the Mevalonate pathway………………….…18
Figure 1.4. Schematic representation of the DOXP-MEP pathway……………………19
Figure 2.1. Schematic representation of the subtractive hybridization
(SH)/cDNA-AFLP approach……………………………………………….…………. 40
Figure 2.2. Example of TDFs in a polyacrylamide gel with one primer combination
(EcoRI+ACT/MseI+CAG). ………………………………………………………..…. 42
Figure 2.3. Validation of the potential resistance gene transcript DL81…………..…..49
Figure 2.4. Validation of the TDFs DL21, DL39 and DL28 using qRT-PCR…….….52
Figure 3.1. Infection of potato by Phytophthora infestans…………………………...69
Figure 3.2. Alignment of deduced amino acid sequences of
Lycopersicon esculentum (LeDXS; AF143812), Medicago truncatula (MtDXS1;
AJ430047), Medicago truncatula (MtDXS2; AJ430048), Oryza sativa (OsDXS;
NM_001062059), and Solanum tuberosum (StDXS1; GU936657). ........…………..….71
Figure 3.3. Phylogenetic tree of DXS proteins from different plant species. ……..…. 74
Figure 3.4. Transcript profiles of genes encoding enzymes in the DOXP-MEP
pathway…………………………………………………………………………………78
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