A Methods Manual for the Collection, Preparation and Analysis of ...

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A Methods Manual for the Collection,Preparation andAnalysis of Diatom SamplesVersion 1.0Report to theWater Research CommissionbyJC Taylor*, WR Harding**and CGM Archibald**** School of Environmental Sciences and DevelopmentNorth-West University (Potchefstroom Campus)** DH Environmental Consulting [DHEC]*** KZN Aquatic Ecosystems [KZNAE]WRC Report TT 281/07January 2007
This report is part of a set on Diatoms. The other report is:WRC report TT 282/07: An Illustrated Guide to Some Common Diatom Speciesfrom South AfricaEach report is provided with a DVD of1. Training Videos for Diatom Field Sampling and Laboratory Practice2. An electronic Diatom Taxonomic Key. Also, see plates in TT 287/07These are obtainable from:Water Research CommissionPrivate Bag X03Gezina, Pretoria0031 South AfricaThe Methods Manual emanates from a Water Research Commission research projectentitled: Development of a Diatom Assessment Protocol (DAP) for River HealthAssessment (WRC project no K5/1588), for which DH Environmental Consulting wasthe Lead Consultant.DISCLAIMERThis report has been reviewed by the Water Research Commission (WRC) andapproved for publication. Approval does not signify that the contents necessarilyreflect the views and policies of the WRC, nor does mention of trade names orcommercial products constitute endorsement or recommendation for use.ISBN 1-77005-483-9SET No 1-77005-482-0Printed in the Republic of South Africaii
Page 1: A Methods Manual for theCollection,
Page 5 and 6: FOREWORDThis method manual is inten
Page 7 and 8: TABLE OF CONTENTSNOTE TO THE USER .
Page 9 and 10: GLOSSARYAerophilic - Depending on f
Page 11 and 12: Phytoplankton - The phytoplankton c
Page 13 and 14: photomicrographs earned high praise
Page 15 and 16: 2. How do you recognise diatoms in
Page 17 and 18: 4. Diatoms - Colony formation and a
Page 19 and 20: 5.1 Pennate and Centric diatomsPenn
Page 22 and 23: SECTION 2: FIELD PROCEDURESTo facil
Page 24 and 25: depending on the period of exposure
Page 26 and 27: 2.3.2 Examination of fresh material
Page 28 and 29: 3. Toolkit for Fieldwork (field app
Page 30 and 31: 5. Site selection for water quality
Page 32 and 33: 6. Sampling locality details and fi
Page 34 and 35: 8. Choice of substrata (detail)Samp
Page 36 and 37: 10 Preservation of diatom material
Page 38 and 39: 1: Micro-pipette 1 ml with disposab
Page 40 and 41: 1.2 Pre-preparation examination of
Page 42 and 43: 1.3.2.3 Shake the sample well and p
Page 44 and 45: one is available the peroxide can b
Page 46 and 47: 2 Preparation of diatom slidesHealt
Page 48 and 49: 2.1.9 After the diatom-coated cover
Page 50 and 51: 4. ArchivingIt is important to reta
Page 52 and 53:
affected at counts of 300 and above
Page 54 and 55:
6. Counting records (electronic and
Page 56 and 57:
The main source of variability is,
Page 58 and 59:
11. Key reference worksThere are se
Page 60:
HARDING WR, ARCHIBALD CGM, TAYLOR J
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