A Methods Manual for the Collection, Preparation and Analysis of ...

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The main source of variability is, however, the identification of individuals, that is whybiological quality controls focus mainly on counts and misidentification. Diatoms are suitablefor such controls and proposals have been made by Kelly (1999).Prygiel et al. (2002) made recommendations which should be seen as the way forward forSouth Africa in terms of quality control and the validation of diatom analysis data:Most of the variability due to sampling and slide preparation can be avoided byorganising comparisons between different studies. Such comparisons are very usefulbecause, with field and laboratory approaches, they make operators aware of theconsequences of not following protocols. They are also useful because they highlightsome taxonomic problems.Diatoms are good subjects for photomicrography and therefore most diatomists use theinternet to check problematic identifications. This approach should be encouraged byformalising expert-practitioner exchanges, by creating iconographic databases, and byorganising regular workshops to allow updating of knowledge.The archiving of permanent slides also facilitates the creation of reference collectionswhich are particularly useful for the identification of difficult species.10. Recommended software packagesPackage Applications/comments Available from:Opticount A counting programespecially designed forthe enumeration of algaespecies and calculation ofbiomass based on speciescounts.DoMix SoftwareDr. Dominik HepperleDorstrasse 20D-18249 Klein RadenGermanyinfo@do-mix.deOmnidiaTaxIBDDiatom-based indexcalculation and archivingboth diatom sampleinformation and speciescounts. This is a veryvaluable tool containingabout 11 000 diatomsspecies with synonymsand references.A pictorial key containingthe 209 species used forthe calculation of theFrench Indice BiologiqueDiatomées (IBD). This isa simple key accompaniedby photographs and ahttp://science.do-mix.de/software.phpCLCIMrs. Catherine Lecointe :Fax: +33 5 53 61 26 73Give your computer type (Mac or PC andWindows version)Payment can be made by cheques orinternational postal orderPRICE: Euros 701,27 (07/2005)clci@club-internet.frhttp://membres.lycos.fr/rimetfrederic/links.htmll'Agence de l'Eau Artois Picardie200 Rue MarcelineBP 81859508 Douai CedexFrance45
CommonFreshwaterDiatoms ofBritain andIreland: Amulti-accesskeyCommonFreshwaterDiatoms ofSouthAfrica: Amulti-accesskey.AnalySISshort description of thetaxa used in the index.This electronic key isbased on LUCID softwareand contains 379 riverdiatom species commonlyencountered in Britainand Ireland. This keyprovides an introductionto the morphology of thediatoms, a detaileddescription of each taxonincluded accompanied byphotographs andecological information.This electronic key isbased on LUCID softwareand contains ~ 200 riverdiatom species commonlyencountered in SouthAfrican fresh waters. Thiskey provides a detaileddescription of each taxonincluded, accompanied byphotographs, drawingsand ecologicalinformationAllows for image capture,measurement, processingand archivingUK Environment Agencyhttp://www.environment-agency.gov.ukWater Research CommissionPrivate Bag X03Gezina0031Mrs Rina WinterPublicationsTelephone: 012 330 0340Fax: 012 331 2565orders@wrc.org.zahttp://www.wrc.org.zaWirsam Scientific & Precision EquipmentMrs. Colleen Syrett23 Menton RoadRichmond 2092JohannesburgTelephone: 011 482-1060Fax: 011 726-6094e-mail: wirsamjb@wirsam.comhttp://www.soft-imaging.net46
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A Methods Manual for theCollection,
Page 3 and 4:
This report is part of a set on Dia
Page 5 and 6: FOREWORDThis method manual is inten
Page 7 and 8: TABLE OF CONTENTSNOTE TO THE USER .
Page 9 and 10: GLOSSARYAerophilic - Depending on f
Page 11 and 12: Phytoplankton - The phytoplankton c
Page 13 and 14: photomicrographs earned high praise
Page 15 and 16: 2. How do you recognise diatoms in
Page 17 and 18: 4. Diatoms - Colony formation and a
Page 19 and 20: 5.1 Pennate and Centric diatomsPenn
Page 22 and 23: SECTION 2: FIELD PROCEDURESTo facil
Page 24 and 25: depending on the period of exposure
Page 26 and 27: 2.3.2 Examination of fresh material
Page 28 and 29: 3. Toolkit for Fieldwork (field app
Page 30 and 31: 5. Site selection for water quality
Page 32 and 33: 6. Sampling locality details and fi
Page 34 and 35: 8. Choice of substrata (detail)Samp
Page 36 and 37: 10 Preservation of diatom material
Page 38 and 39: 1: Micro-pipette 1 ml with disposab
Page 40 and 41: 1.2 Pre-preparation examination of
Page 42 and 43: 1.3.2.3 Shake the sample well and p
Page 44 and 45: one is available the peroxide can b
Page 46 and 47: 2 Preparation of diatom slidesHealt
Page 48 and 49: 2.1.9 After the diatom-coated cover
Page 50 and 51: 4. ArchivingIt is important to reta
Page 52 and 53: affected at counts of 300 and above
Page 54 and 55: 6. Counting records (electronic and
Page 58 and 59: 11. Key reference worksThere are se
Page 60: HARDING WR, ARCHIBALD CGM, TAYLOR J
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