A Methods Manual for the Collection, Preparation and Analysis of ...

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3. Toolkit for Fieldwork (field apparatus)Plastic tray / clean 2 litre ice cream dishes with lidsTooth brush or other similar brushKnife or spoonEnvelopes (for soil samples)Wide mouth sample bottles (~100 ml)“Zip-lock” type plastic bagsPlankton netWaterproof marking pen or labelsField note book/field record formsPipettesDepth gauging ‘broomstick’ / rod100 ml syringe fitted with latex tubing / Turkey basterClear Perspex tubes (25 mm diameter)Camera1: Wide-mouth sampling bottle ~ 100 ml. 2: Turkey baster; useful for collecting sediment samples.3: Pencil and labels, ethanol does not dissolve pencil markings. 4: Forceps for picking upfilamentous algae and detritus. 5: Water-proof marking pen. 6: Plastic Pasteur-pipette useful forcollecting small amounts of sediment. 7: Toothbrushes for scrubbing solid substrata. 8: Knife forcutting the stems of aquatic vegetation. 9: White plastic tray with lip. 10: Fine-mesh plankton net.17
4. Decision ‘tree’ for sampling for water quality monitoringSampling decision treeNarrow wadeablestreamYesSample in the centreof the streamSELECT LOCALITYSAMPLINGPROCEDUREBroad deep riverYesSample near to thebank in an area ofaccelerated f lowNote: All substrata shouldhave been submerged for aperiod of a least 4 weeksYes1.Cobbles, boulders orpebblesNot present / Nonrepresentativeof substratum,proceed with alternativesubstrata in order ofpreferenceYesSubstratum selectionFree from marcophyticalgal growthNoRefer to Table 1 (FieldProcedures, Section 6)TAKESAMPLEYesFree fromsedimentNoRecord degreeof sedimentationYesTAKESAMPLETAKESAMPLE2. Man made objects;bricks, concreteNoYesProceed as for substratum 1TA KESAMPLE3.Bridge supports,channel wallsYesTAKESAMPLENo4.Emergent aquaticmacrophytesYesTAKESAMPLENo5. Submerged aquaticmacrophytesYesTAKESAMPLENo6.Sediment substrataYesTAKESAMPLE7. No suitablesubstrata at siteIntroduce artificialsubstrataWait for at least 4weeksTAKESAMPLE18
Page 1 and 2: A Methods Manual for theCollection,
Page 3 and 4: This report is part of a set on Dia
Page 5 and 6: FOREWORDThis method manual is inten
Page 7 and 8: TABLE OF CONTENTSNOTE TO THE USER .
Page 9 and 10: GLOSSARYAerophilic - Depending on f
Page 11 and 12: Phytoplankton - The phytoplankton c
Page 13 and 14: photomicrographs earned high praise
Page 15 and 16: 2. How do you recognise diatoms in
Page 17 and 18: 4. Diatoms - Colony formation and a
Page 19 and 20: 5.1 Pennate and Centric diatomsPenn
Page 22 and 23: SECTION 2: FIELD PROCEDURESTo facil
Page 24 and 25: depending on the period of exposure
Page 26 and 27: 2.3.2 Examination of fresh material
Page 30 and 31: 5. Site selection for water quality
Page 32 and 33: 6. Sampling locality details and fi
Page 34 and 35: 8. Choice of substrata (detail)Samp
Page 36 and 37: 10 Preservation of diatom material
Page 38 and 39: 1: Micro-pipette 1 ml with disposab
Page 40 and 41: 1.2 Pre-preparation examination of
Page 42 and 43: 1.3.2.3 Shake the sample well and p
Page 44 and 45: one is available the peroxide can b
Page 46 and 47: 2 Preparation of diatom slidesHealt
Page 48 and 49: 2.1.9 After the diatom-coated cover
Page 50 and 51: 4. ArchivingIt is important to reta
Page 52 and 53: affected at counts of 300 and above
Page 54 and 55: 6. Counting records (electronic and
Page 56 and 57: The main source of variability is,
Page 58 and 59: 11. Key reference worksThere are se
Page 60: HARDING WR, ARCHIBALD CGM, TAYLOR J

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