6970EFM Freezer/Mill Cryogenic Grinder - SPEX SamplePrep

Grinding andPulverizingTissueHomogenizationCell LysisEXTERNAL LOADING : :ENCLOSED SYSTEM WITH LIQUID NITROGEN AUTOFILL : :HIGH THROUGHPUT : :MULTIPLE VIAL SIZES : :

FIELDS OFAPPLICATION: :Biotechnology: :Plant Researchand Crop Science: : Pesticide Residues: : Medicin e andPharmaceuticals: : Marine Biology: : Forensics: :Polymers: : RoHS WEEE: : Food Science: : Materials Science: : Nanotechnology andMechanical Alloying: : Glass and Ceramics: : Geology and Mining: : Environmental Science: : Cement, Slag and Fly Ash: : Catalysts: : National Defense andHomeland Security: : Veterinary Drugs6970Dincludes two grinding chambers, increasing grinding capacity to eight 6751grinding vials. Chambers can be programmed for independent operation anderent protocols, providing exibility and speed.6970Mincludes one grinding chamber and one pre-cool/storage chamber.THE LATEST ADVANCES IN CRYOGENIC GRINDING: : For samples that are impossible to grindat ambient temperature: : Backed by SPEX SamplePrep’s 40 yearsof experience in cryogenic grinding: : Ideal for grinding bone, teeth, hair,tough plant and animal tissue, textiles,plastics, pharmaceuticals and more: : DNA/RNA extraction without shearing: : No loss of volatile compoundsAccuracy: : Samples are maintained at LiquidNitrogen (LN) temperatures: : Vials can be cleaned or discarded toeliminate possibility of contaminationSafety and Convenience: : Totally enclosed LN auto ystem withno LN exposure: : CE compliantDurability: : Unique maintenance free design, onlyone moving partVersatility: : Entire system is controlled by a touchscreen interface capable of storing up to10 grinding programs for rapid processreplication and consistency: : Grinds samples from 0.1-100 grams pergrinding chamber (depending on samplecharacteristics)For more information please call 855-GET-SPEX or visit www.spexsampleprep.com/freezermill

ACCESSORIESSTANDARD GRINDING VIALS: : Sample integrity ismaintainedin tightly sealed vials: : Polycarbonate6751 grinding vialscenter cylinders withsteel end plugs andimpactors: : Polycarbonatecenter cylinders arereusable or can beused as disposables: : Three sizes: small samples up to 4mL, mid-sizesamples for up to 25mL, and large samples for up to 50mL: : Up to four small vials per grinding chamberusing multi-vial adapterSPECIAL GRINDING VIALS: : Alternative materials available formost vial types: : Poly-Vial sets for grinding softtissues without metalcontamination: : Cr-Free Vial set for RoHS/WEEEtesting of electronic components: : Micro-Vial set holdsthree micro-vials forsamples from 0.1-0.5mL: : Steel Center Cylindersfor when contactwith plastic is notadvisedCr-Free ImpactorPoly VialCr-Free vialVIAL EXTRACTORS AND VIAL RACKS: : Vial extractors are used to remove endplugs from center cylinder: : Free vial extractor and rack accessorypackage supplied with each 6970Freezer/MillAUTO _ EXTRACTOR : : The 6814 Auto-Extractor is anelectrically powered version ofthe manual vial extractors: :increases throughput by speedingup end plug removal: : Works with large and small vialsSPEX SAMPLEPREP OFFERS THE COMPLETE SOLUTION FORAUTOMATED CRYOGENIC GRINDING: : Cryogenic Transfer Hoses to connectDewar to theFreezer/Milloutlet in the: : Cryogenic gloves-designed to protect thehands and arms from ultra-cold materials

B E F O R E & A F T E R S A M P L E SABSTRACTSBiotechnology & ForensicsRNA Extraction from Knee Cartilage TissueA study to determine the connection between arthritic diseasesand certain genes was conducted. Knee cartilage tissue wastaken from patients and snap frozen in liquid nitrogen for storage.Samples were ground in the large Freezer/Mill using small vialsand a multi-vial adapter. Grinding was completed in three cyclesof two minutes each. The total processing time of 12 minutesincluded precool, grinding, and cooling between grinding cycles.RNA was subsequently pur ed using a phenol-chloroformextraction method with yields of RNA between 2 & 20 µg.Polymers & Materials ResearchA P P L I C A T I O N N O T E SGrinding Polymers for Qualitative and Quantitative AnalysisCompact synthetics (pellets, webbing fabric, textiles) andpolymer yarn ce material and laments were successfullyground in the small Freezer/Milll. Cryogenic grinding wasrequired for accurate qualitative and quantitative analysis ofadditives such as antioxidants using HPLC and INOC-Test. TheFreezer/Mill iently reduced these samples to particle sizes ofless than 500 µm in less than 15 minutes without thermal degradation.Examples: : SP005 – RNA Extraction from CartilageTissue using Cryogenic Grinding: : SP006 - Preparation of Rice Husks forContent Analysis: : SP007 - Grinding Polymers for Qualitativeand Quantitative Analyses: : SP015Cetacean Samples (RNA Extraction fromWhale Skin and Blubber)Crop ResearchPreparation of Rice Husks for Content AnalysisA study to analyze the quantity of vitamins and importantminerals in rice husks was performed. 8g of sample was groundin the large Freezer/Mill. Rice husks are extremely tough and in3 grinding cycles of 3 minutes each, very good homogenizationof the rice husks was achieved. The analytical neness of thehomogenized sample enabled cient extraction of the desiredcomponents for analysis.Please visitwww.spexsampleprep.com/appnotesfor more FREEZER/MILL application notes.Customer ServiceSampleprep@spex.com15 Liberty Street , Metuchen, NJ 08840732-623-0465 • Fax 732-906-2492www.spexsampleprep.com

THE ULTIMATE PLANT & ANIMAL TISSUEPULVERIZER & CELL LYSERThe 2010 Geno/Grinder: :titer plates and sample vials from 0.6 to 50mL: : Used to prepare tissue for extractions of nucleic acid, protein, and otherconstituents such as pesticides: : Sample material includes seeds, stems, roots, leaves and soft animal tissue: : Samples are pulverized with a large choice of grinding media, with or: : Unique vertical motion with variable rate settings for mixing andpulverizing samplesFor more information on the Geno/Grinder, please visit:www.spexsampleprep.com/genogrinderPatentedUS HeadquartersSPEX SamplePrep15 Liberty StreetMetuchen, NJ 08840-9978Tel: 732.623.0465Fax: 732.906.2492855.438.7739 (855.GET.SPEX)E-mail: sampleprep@spex.comwww.spexsampleprep.comEuropean DivisionSPEX CertiPrep Ltd2 Dalston GardensStanmore, HA7 1BQ, UKTel: +44 (0)20 8204 6656Fax: +44 (0)20 8204 6654E-mail: sales@spex.comwww.spexsampleprep.comD E M O P R O G R A M : T R Y I T B E F O R E Y O U B U Y I TSPEX SamplePrep gives you the opportunity to try out oursampl e preparation equipment in your laboratory for one week.Best of all, our Demo Program is COMPLIMENTARY!!!For moreinformation pleas e call 1-855- GET -SPEXor visit www.spexsampleprep.com/freezermillOnce you try our quality products you’ll ask yourself how youever lived without . them.© 2012 SPEX Sample