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<strong>BUITEMS</strong> Quality & Excellence in Education Extraction Of Active Components Of Aloe Vera To Treat Acne/Pimple on skin burns, moisturizing dry skin and it is taken internally for peptic ulcers or gastritis. (Rabe and Staden, 1997). Chemical investigations of different parts of leaf The detailed chemical investigation of Aloe Vera distinguishes between the different parts of the leaf. The leaf pulp separately analyzed from the rind. The pulp was found to contain 98.5% water, and its alcoholinsoluble was mucilage with high content of uronic acid, fructose and hydrolysable sugars. Enzymes such as an oxidase, a catalase, a cetalase and an amylase were reported to be present, but small nitrogen content. The leaf pulp did not respond to tests for “aloin”. The chemical components of the gel scraped from the leaves after the exudate had been drained off, were examined. The crude gel had an ash content of 12.9%, removable by dialysis. White water-soluble mucilage was purified which on hydrolysis was found to contain equal amounts of glucose and mannose as the main constituents, with a small amount of uronic acid (2.37%). Ketoses were thought to be absent. They also reported that some aloin was present in the crude gel, despite bleeding off the exudates from the leaves. MATERIALS AND METHODS Collection of plant material Pakistani and Irani Aloe vera leaves were collected from the Botanical garden of Balochistan University, Quetta, Balochistan, Pakistan. Test organisms Pure cultures of bacterial strains of Escherichia coli, Staphylococcus aureus, Bacillus subtilius, and Salmonella typhie were used as test organisms. The organisms were maintained on agar slants stocks and were subsequently subcultured into newly prepared macConky agar slants. Plant extraction and fractionation The leaves (2 kg) each were dried in shade, ground and soaked in ethanol for 10 days separately. Thick dark crude extracts were yielded after evaporating the solvent through rotary evaporator. Fractionation of the methanolic extracts was carried out to find the antibacterial activities. The extracts were dissolved in Di-Methyl Sulfoxide (DMSO) and used for the antimicrobial susceptibility testing. Antimicrobial susceptibility testing The antibacterial activities of four plants extract were determined against bacterial strains by agar well diffusion Technique (Agarry et al; 2005). Sterile agar (at 45°C) was poured into sterile Petri dishes, which had been inoculated with the test organisms. The plates were allowed to gel for an hour. Wells (10 mm diameter) were made with the aid of flamed cork borer on the surface of the agar plates. 0.1 gram of ethanol extracts of aloe vera plants were dissolved in (DMSO).From this 10 μl, 15 μl was pipette out and added to the respective wells. These were incubated at 37°C for 24 h. The presence of zones of inhibition was regarded as the presence of antimicrobial action. From the inhibition zones seen, antimicrobial activity was expressed in terms of average diameter of the zones of inhibition measured. RESULTS AND DISCUSSION Different doses for 75% Aloe vera solution gave variety of results. Doses of 0.1, 1.0, 1.5, 2.0 and 2.5 didn’t give any result on acne vulgaris caused by propionibacterium acnes on body of mice for 3 days. 3ml does for 3 days caused the diameter of lesion to reduce about 4.5 mm, 3.5ml does for 3 days caused the diameter of lesion to reduce about 4.3 mm, 4ml does for 3 days caused the diameter of lesion to reduce about 4.1 mm, 4.5ml does for 3 days caused the diameter of lesion to reduce about 3.9 mm best results were obtained at 5ml does for 3 days caused the diameter of lesion to reduce about 3mm. (Table 1, Figure 2). Different doses for 80% Aloe vera solution give variety of results. 0.1ml does for 3 days caused the diameter of lesion to reduce about 4.6 mm, 1ml does for 3 days caused the diameter of lesion to reduce about 3.9 mm. 1.5ml does for 3 days caused the diameter of lesion to reduce about 3.2 mm, 2ml does for 3 days caused the diameter of lesion to reduce about 2.2 mm, 2.5ml does for 3 days caused the diameter of lesion to reduce about 1.45 mm, 3ml does 66
<strong>BUITEMS</strong> Quality & Excellence in Education Extraction Of Active Components Of Aloe Vera To Treat Acne/Pimple for 3 days caused the diameter of lesion to reduce about 1.15 mm, 3.5ml does for 3 days caused the diameter of lesion to reduce about 0.9 mm, 4ml does for 3 days caused the diameter of lesion to reduce about 0.65 mm, 4.5ml does for 3 days caused the diameter of lesion to reduce about 0.45 mm, 5ml does for 3 days caused the diameter of lesion to reduce about 0.3 mm. so the best results were obtained at 5ml in 80% aloe Vera concentration. (Table 2, Figure 3). (Reynolds and Dweck, 1999). Different doses for 85% Aloe vera solution give variety of results. first dose of 0.1ml for 3 days caused the diameter of lesion to reduce about 4.4mm, second dose of 1ml for 3 days caused the diameter of lesion to reduce about 3.5mm, third dose of 1.5ml for 3 days caused the diameter of lesion to reduce about 2.9mm, fourth dose of 2ml for 3 days caused the diameter of lesion to reduce about 2.1mm, fifth dose of 2.5ml for 3 days caused the diameter of lesion to reduce about 1.2mm, sixth dose of 3ml for 3 days caused the diameter of lesion to reduce about 0.8mm, seventh dose of 3.5ml for 3 days caused the diameter of lesion to reduce about 0.5mm, eighth dose of 4ml for 3 days caused the diameter of lesion to reduce about 0.2mm, ninth dose of 4.5ml for 3 days caused the diameter of lesion to reduce about 0.1mm, tenth dose of 5 ml for 3 days eliminated all kind if lesions with improved texture of skin. (Table 3, Figure 4). Different doses for 90% Aloe vera solution give variety of results. first dose of 0.1ml for 3 days caused the diameter of lesion to reduce about 4.2mm, second dose of 1ml for 3 days caused the diameter of lesion to reduce about 3.1mm, third dose of 1.5ml for 3 days caused the diameter of lesion to reduce about 2.4mm, fourth dose of 2ml for 3 days caused the diameter of lesion to reduce about 1.9mm, fifth dose of 2.5ml for 3 days caused the diameter of lesion to reduce about 1.1mm, sixth dose of 3ml for 3 days caused the diameter of lesion to reduce about 0.6mm, seventh dose of 3.5ml for 3 days caused the diameter of lesion to reduce about 0.3mm, eighth dose of 4ml for 3 days caused the diameter of lesion to reduce about 0.1mm, ninth dose of 4.5ml for 3 days eliminated all kind if lesions with improved texture of skin(Rubina et al., 2009). (Table 4, Figure 4) Table 1: Results for 75% dose of Aloe vera solution Dose (ml) Size (mm) Other effects 0.1 NIL NA 1.0 NIL NA 1.5 NIL NA 2.0 NIL NA 2.5 NIL NA 3.0 4.5 Slight decrease in redness, no affect on pus filled lesions and hard cysts 3.5 4.3 Dryness of pus filled lesions, no affect on hard cysts 4.0 4.1 Redness disappeared completely, pus filled lesions healed, no affect on hard cysts 4.5 3.9 Hard cysts softened 5.0 3 Decrease in lesion’s size and inflammation 67
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