Recombinant DNA Technology
Recombinant DNA Technology
Recombinant DNA Technology
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Three Activities of E. coli <strong>DNA</strong>P I (1)<br />
(1)<br />
• Polymerization of dNTPs<br />
at the 3’end of the growing<br />
chain (1)<br />
• 5’exonuclease removes<br />
nucleotides from 5’end of<br />
chain immediately<br />
upstream of growing chain<br />
(2)<br />
• 3’exonuclease removes<br />
unpaired nucleotides from<br />
3’ end of growing chain