Recombinant DNA Technology
Recombinant DNA Technology
Recombinant DNA Technology
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c<strong>DNA</strong><br />
Synthesis<br />
• Oligo(dT) primer<br />
• Reverse transcriptase<br />
• Klenow/<strong>DNA</strong>P I<br />
• RNase H<br />
• Degrades RNA of<br />
<strong>DNA</strong>:RNA hybrid<br />
• S1 nuclease<br />
• Degrades ss nucleic<br />
acids (unpaired<br />
loop)