Recombinant DNA Technology

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Phage λ cloning vector • Internal segment deleted (now requires helper phage to replicate) • Has cos sites intact • Target DNA inserted between the two λ “arms” (up to about 20 kb) • DNA packaged in vitro • Recombinant phage infect E. coli cells
Cosmid Cloning System λ cos sites inserted into a small plasmid Target DNA ligated between two cosmid DNA molecules Recombinant DNA packaged and E. coli Infected as before Can clone DNAs up to 45 kb
Page 1 and 2: Recombinant DNA Technology • Comm
Page 4 and 5: DNA Cleavage By Restriction Endonuc
Page 6 and 7: Restricti Mapping Restriction Endon
Page 8 and 9: Restriction Endonuclease Cleavage M
Page 10 and 11: Annealing of Complementary “Stick
Page 12 and 13: Early Cloning Vectors • pBR322
Page 14 and 15: Other Plasmid Cloning Vectors • N
Page 16 and 17: Antibiotics Commonly Used as Select
Page 18 and 19: Partial Restriction Endonuclease Di
Page 20 and 21: Genome Sizes of Various Organisms
Page 22 and 23: Production of Labeled Probes Random
Page 24 and 25: Three Activities of E. coli DNAP I
Page 26 and 27: Screening by Immunological Assay
Page 28 and 29: Isolation of Poly(adenylated) mRNAs
Page 30 and 31: Enriching for Full adapter Length c
Page 32 and 33: Inert Capacities Common Vector Syst
Page 34 and 35: Packaging of λ Chromosomes • Nat
Page 38 and 39: High-Capacity Bacterial Vector Syst
Page 40 and 41: Electroporation • Cells suspended
Page 42: Tripartite Mating • P. putida dif

Recombinant DNA Technology

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