October 17-21 2015 Baltimore Maryland

were collected. We used microanalytical assays to measure the levels of ATP
and citrate in single oocytes. To evaluate spindle and chromosome alignment,
mice were injected with PMSG and hCG, sacrificed and ovulated meiosis II
(MII) oocytes were collected. Mature oocytes were stained with tubulin and
DAPI. Imaging was performed on a Leica confocal microscope and analysis
performed blindly with ImageJ software. ANOVA, students t-tests, and chisquare
analysis were used in the statistical analysis as appropriate.
RESULTS: HF mice weighed significantly more than mice on the control
diet (28g vs 21.7g, p