October 17-21 2015 Baltimore Maryland

O-56 Monday, October 19, 2015 11:30 AM
COMPARISON OF A BENCHTOP INCUBATOR AND A TIME
LAPSE INCUBATOR FOR CULTURE OF HUMAN EMBRYOS:
IMPACT OF CULTURE DISH. R. L. Krisher, a C. Pospisil, b
A. F. Greene, a J. M. Stevens, b W. B. Schoolcraft, c J. E. Swain. d a National
Foundation for Fertility Research, Lone Tree, CO; b Fertility Labs of Colorado,
Lone Tree, CO; c Colorado Center for Reproductive Medicine, Lone
Tree, CO; d Fertility Lab Sciences, Lone Tree, CO.
OBJECTIVE: Much debate exists as to the optimal embryo culture incubator.
However, several variables exist that must be controlled to properly
examine the impact of the incubator itself, including the culture dish utilized
and the gas atmosphere. Our objective was to compare human embryo development
between two modern embryo culture incubators; the EmbryoScopeÔ
and the benchtop G185.
DESIGN: Prospective sibling embryo split
MATERIALS AND METHODS: Zygotes from 26 patients (average age
36.70.7) were placed individually into EmbryoSlideÔ dishes (25 mL under
oil). One EmbryoSlideÔ was placed into the EmbryoScopeÔ (Vitrolife,
n¼172 2PNs), while the other was placed into a G185 incubator (K-Systems,
n¼158 2PNs). Embryos were cultured in sequential media for 6 days. Both
incubators utilized 7% CO2, 5% O2. To examine the impact of the dish,
zygotes from 8 patients (average age 33.10.9) were randomly assigned to
culture in either an EmbryoSlideÔ (n¼58 2PNs) or to standard group microdrop
culture (up to 5 embryos per 30mL; n¼73 2PNs) and cultured in a G185
incubator.
RESULTS: Significantly more embryos developed to good quality day 5
blastocysts (>3BB) in the EmbryoSlideÔ when it was incubated in the
G185 benchtop incubator compared to the EmbryoScopeÔ (39.2% versus
28.5%, respectively; p¼0.039), although there was no difference in the number
of good quality blastocysts developing overall by day 6 (54.4% vs.
52.3%, respectively). When cultured in the same incubator (G185), the EmbryoSlideÔ
Supported good quality blastocyst development equal to that of
microdrop group culture (EmbryoSlideÔ- 25.9% day 5 and 58.6% overall;
group microdrop- 26.0% day 5 and 58.9% overall).
CONCLUSIONS: In patient embryo splits with a controlled gas atmosphere
and culture dish, the benchtop G185 incubator yielded more high
quality day 5 blastocysts compared to the EmbryoScopeÔ. The EmbryoSlideÔ
dish supports blastocyst development of individually cultured human
embryos equally well compared to group culture in standard
microdrops within the benchtop G185. Using sequential media and low oxygen,
modern benchtop incubators function as well as time-lapse incubators
and permit individual embryo culture, which is critical for the application of
biomarker analysis.
O-57 Monday, October 19, 2015 11:45 AM
PATIENT TREATMENT JOURNEYS, THEN AND NOW: 10 YEARS
OF CLINICAL PROGRESS?. A. Zgodic, a H. Karvir, a D. Parfitt, a
S. T. Globus, a A. B. Copperman, b P. Yurttas Beim. a a Celmatix Inc, New
York, NY; b Reproductive Medicine Associates of New York, New York, NY.
OBJECTIVE: As assisted reproductive technology (ART)-based therapies
are refined and clinical expertise is strengthened over time, improvements
are expected in live-birth outcomes for patients beginning
infertility treatment. To investigate this hypothesis, we analyzed trends in
clinical outcomes over a ten-year period at a large academic reproductive
medical center.
DESIGN: Retrospective analysis using de-identified ART cycles (10801
in-vitro fertilization (IVF) cycles, 1462 Egg Recipient (ER) cycles, and
33947 Non-IVF (NIVF) cycles) from 2003-2012.
MATERIALS AND METHODS: We performed trend analyses within:
1) All; 2) IVF; and 3) NIVF cycles. We used two-sided chi-square test to
investigate significant trends in live birth and multiples rates. The direction
and magnitude of these trends were assessed using a Binomial model with
treatment year as a predictor. For mean time-to-live birth (months), mean
cost of a singleton live birth, mean time between cycles (months), and
mean number of embryos transferred (IVF only), we used a linear model
to detect significant trends.
RESULTS: In all cycles, we observed no significant trends in odds of
achieving live birth or multiples (p