Products at a Glance 2024

More than competence for cells 

Products 

at a glance 

Your Shortcut to Products 

from Tissue Dissociation 

to Cell-based Assays 

www.pelobiotech.com

www.pelobiotech.com 

Rely on & Relax 

Research grade products 

GMP grade products 

Welcome to your One-Stop-Cell Culture Shop, 

having more than competent 4 cells – that is PELOBiotech’s outstanding characteristic. We want you to get the best 

and most reproducible results ever, results you can rely on and relax. We offer special cell culture media for human 

primary cells, stem cells and embryonic stem cells as well as special supplements for tumor stem cells. We get you the 

whole variety of solutions from tissue dissociation to cryo-preservation. 

Our mission is to provide high-quality cell culture products at affordable prices. Therefore, we are at the top-level of 

international research regarding consistent quality. We block out the Black Box esp. while developing Defined or 

Xeno-free Media limiting usage of FBS and other animal products. We have built up a worldwide network of 

competent and innovative partners. Our network clears your direct access to products of the future research. 

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Our products at a glance 

• Tissue Dissociation 

• Cells & Tissues 

Collagenases | Proteases | Enzyme Blends 

iPS Cells & iPS derived Cells | Adult Stem Cells (Hematopoietic Stem 

Cells) | Mesenchymal Stroma/Stem Cells | Primary Cells | Organ derived 

Primary Cells | Blood & Bone Marrow derived Cells (MNCs & Immune 

Cells) | Tissue Slices | Cell and Tissue RNA 

• Cell Culture Media for the cells mentioned above 

FBS | hPL | animal origin free | defined media 

• Cell Culture Tools 

• 3D Cell Culture 

• Essential extras 

• Assays 

Cryo & Cold management | Cell Culture supplements | 

Extracellular Matrices | Cell Culture reagents | Transfection Tools | 

Reprogramming Tools 

Scaffold-free | Scaffold-based | Hydrogel-based | 

3D Models Ready-to use | Midifluidic 

Antibodies | Dyes | Proteins | Europium Chelates | 

TR-FRET Reagents | Molecular biology Reagents | 

Small Molecules | Product Purification Tools | Safety Tools 

Angiogenesis Assays | Cell-based Assays | Cell Purity Kit | 

Chimerism Assays | Metallo Quantification Assays | Metastasis Assays 

*Illustrations Created with BioRender.com even if its not mentioned everywhere 

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Tissue Dissociation 

Tissue Dissociation is an extremely important early step in experiments using primary cells. This early step already 

determines the quality of your cell culture and finally your results. 

The main question is how to isolate cells in the best way and which criteria are the most important: Is it the cell 

viability, the biomarker profile or just the cell number? Our partner VitaCyte offers only highly purified tissue 

dissociation enzymes for basic research as well as for cell therapy. 

CIzyme TM Tissue Dissociating Enzymes 

Advantages of Purified Enzymes for Cell Isolation 

Problems with crude or enriched collagenase: 

Variable biochemical composition, reflects 

heterogeneity of the bacterial culture supernatant 

• Heterogenous molecular forms of 

collagenase Lot qualification often 

required 

Collagenases and Proteases 

Advantages of using purified enzymes: 

Uniform collagenase composition, by HPLC and 

specific CDA1 (CDA U/mg protein) 

• Purified enzymes; minimal contamination 

by other enzymes & endotoxin 

• Enzyme formulation can be modified to 

improve cell yield, viability, function 

Collagenases and Proteases 

Applications – Isolation of 

• Hepatocytes 

• Islet Cells 

• Adipose Tissue Stem Cells 

• Skin Fibroblast 

• Skin Keratinocytes 

• Cardiomyocytes 

• Glioblastoma 

NEW Products: 

Recombinant Collagenase 

Defined and enriched DE Collagenase products. 

Collagenase is blended with purified neutral protease 

at defined activities to provide a broad spectrum of 

products with distinct collagenase activity. 

Pic: High viable 

cell yields: Get 

consistent results 

with our enzyme 

formulations 

4

U/mg DW 


PD Collagenases 

Changing the paradigm. 

VitaCyte’s purified defined (PD) collagenase products 

offer a new standard of versatility in optimizing cell 

isolations. Enriched collagenase is blended with purified 

neutral protease at defined activities to provide a 

broad spectrum of products with distinct collagenase 2,3 to 

neutral protease 1 activities. This design feature simplifies 

selection of an optimum ratio of critical enzyme activities 

for each cell isolation application. This manufacturing process minimizes 

enzyme variability enabling consistent control of cell isolations. 

18000 

16000 

14000 

12000 

10000 

8000 

6000 

4000 

2000 

0 

Purified defined Collagenase 

PD 100 PD 800 

CDA U/mg NP U/mg Clostripain U/mg 

Each PD product contains a fixed amount of protease activity (NP U 1 ), low clostripain activity 4 , and increasing amounts of 

collagenase activity defined by a functional collagen degradation activity (CDA U) that correlates with the 

biochemical form of the enyzme 2 . The target collagenase: protease activity ratios enables efficient 

exploration of a broad range of formulations. 

The three Cs of PD Collagenase 

CONSISTENT CONVENIENT COST EFFECTIVE 

• Fixed amount of purified protease 

• Low clostripain contamination 

• Removal of the majority of 

contaminants in crude collagenase 

• • Weigh out what you need or 

reconstitute and use entire bottle 

• • Rapid dissolution, no clogged 

filters, no need for pre-centrifugation 

• • Stable for 2 years at +4°C 

storage, 4 years at < -20°C 

• • Increase productivity, minimize need 

for lot testing 

• • Purchase of large quantities of “good” 

lots of collagenase avoided 

• • Easily migrate to purified enzyme products 

based on enzyme activity analysis of 

DE Collagenase 


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Ask for PD Collagenase! 

The products below are defined by the total Wünsch activity per bottle, an assay that uses a peptide substrate to measure 

the activity of the collagenase catalytic domain. This activity does not provide functional assessment of collagenase activity 

but it is one of the most consistent and reliable activity measures used by many collagenase suppliers. The table below 

provides ordering information and a cross reference chart to indicate which PD Collagenase product may meet your needs. 

The Collagenase Gold product is similar to the PD Collagenase 800 but does not contain any supplemental purified 

protease. This product enables users to use other proteases or to purchase from VC the same protease used in the PD 

Collagenase products (BP Protease, catalog # 003-1000) for creation of a custom mixture. 

Product Catalog # #/Quantity Cross Reference 

PD Collagenase 100 

PD Collagenase 800 

Collagenase Gold 

PB- 011- 

1010 

PB-011- 

1050 

PB-011- 

1060 

1 g 

Worthington Type 1 or 2 Liberase TM or DH, 

Blendzyme 1 or 2 

1 g Liberase TL, Liberase DL, Liberase H I 

1 g Human Islets 

To isolate sensitive cells such as islets, high purity and fidelity collagenase are essential due to their critical role in enzymatic 

degradation of the extracellular matrix without damaging the structural and functional integrity of the cells. Successful 

human islet isolation requires the use of defined collagenase-protease enzyme mixtures, and intact class I and class II 

collagenase should be used to ensure maximal islet recovery at the lowest dose of enzyme. This is why Collagenase Gold 

is ideal for islet isolation. Thereby the use of these enzymes which are GMP and AOF can help with the best results. It has 

been found that excess collagenase may potentially harm islets during the isolation process, emphasizing the need for 

precise dosing and high purity enzymes to avoid detrimental effects on the isolated cells. Furthermore, studies have 

demonstrated that islet mass, quality, and purity are determined by the efficacy of islet isolation, which depends on factors 

such as organ procurement, collagenase digestion, and purification method. This highlights the critical role of high purity 

collagenase in ensuring the quality and purity of isolated islets. 

References 

1. Breite AG, et al. Transplantation Proceedings 42 (2010); 2052-2054. 

2. McCarthy RC, et al. Transplant Proc 40 (2008); 339-342. 

3. Wünsch E and Hedrich H-G. Hoppe-Seyler’s Zeitschrift Physiol Chemie 333 (1963);149-151. 

4. Mitchell WM and Harrington WF. Methods in Enzymology (1970) 635-642. 

5. McCarthy RC, et al. Transplant 91 (2011) 137-145. 

Scan the QR code to visit the VitaCyte website to get additional protocols and product 

information. 

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CIzyme TM Products 

Product Catalog # Quantity App Quality 

CIzyme TM Collagenase HA PB-001-1000 2000 Wünsch units Human islets GMP 

CIzyme TM Collagenase HA PB-001-1050 200 Wünsch units Rodent islets GMP 

CIzyme TM Collagenase MA PB-001-2020 1100 Wünsch units Porcine islets GMP 

CIzyme TM Collagenase MA PB-001-2030 2,5 Mill CDA units Human hepatocytes GMP 

CIzyme TM rCollagenase HI PB-001-4010 1600 Wünsch units Human islets 

GMP & 

AOF 

CIzyme TM Collagenase Gold PB-011-1060 800 Wünsch units Human islets 

CIzyme TM Collagenase Gold 

Plus 

PB-011-2000 

>1,500 FALGPA units 

Human islets from nondiseased 

organs 

GMP 

CIzyme TM Thermolysin PB-002-3000 6 mg Protease 

GMP & 

AOF 

CIzyme TM BP Protease 

PB-003-1000 

1,1 mill neutral protease 

units 

Neutral Protease 

GMP & 

AOF 

CIzyme TM BP Protease 

PB-003-2000 

2,3 mill neutral protease 

units 

Neutral Protease 

GMP & 

AOF 

Clostripain PB-004-2000 25 mg Protease GMP 

CIzyme TM RI PB-005-1030 375k CDA U, 75K NP U Rodent islets 

CIzyme TM AS PB-005-1090 35 W U, 280k NP U Adipose derived stem cells GMP 

CIzyme TM Hepatocyte 

Isolation Kit 

PB-005-1010 

2,5 Mill CDA U,2,2 Mill NP 

U mg 

Hepatocytes 

GMP 

PD Collagenase 100 PB- 011- 1010 1 g 

PD Collagenase 800 PB-011- 1050 1 g 

Porcine & rodent 

hepatocytes 

Rodent dendritic cells, rat 

cardiomyocytes, fibroblasts 

and porcine kidney 

epithelial cells 

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Cells & Tissues 

iPS Cells 

Induced Pluripotent Stem Cells (iPSCs) have revolutionized the field of regenerative medicine and scientific research by 

offering a versatile platform for studying and harnessing the potential of stem cells. These remarkable cells, pioneered 

by Dr. Shinya Yamanaka, are capable of differentiating into a wide range of cell types, mirroring the attributes of 

embryonic stem cells. We offer a wide range of reprogramming tools, recombinant proteins and small molecules for this 

application. Recombinant proteins, synthetic transcription factors, enhance targeted reprogramming, while small 

molecules, such as valproic acid, optimize efficiency and maintain pluripotency. Additionally, the choice of an appropriate 

coating for culture vessels such as recombinant Laminin is crucial in supporting iPSC attachment, proliferation, and 

differentiation. 

Every cell type will have a variant of integrin and thereby you will require a specific laminin for each cell type, we 

recommend you check out our ECM solutions i-Matrix 211, 411, 511. This integrated approach, including proper 

coating considerations, streamlines iPSC generation, addressing safety concerns and making them a valuable resource 

for scientific and regenerative applications. 

• Ready-to-use iPSCs crafted from different techniques such as footprint-free StemRNA 3rd Gen Reprogramming 

Technology, Retrovirus & Sendai virus. 

• No need for specialized reprogramming expertise. 

• Available from both males and females, complete with donor clinical status. 

• Sourced from skin fibroblasts and blood endothelial progenitor cells. 

We would like to point out to you as an interested party in genetically modified cells (e.g. iPS cells, GFP/RFP 

tagged cells) that you have a corresponding authorized genetic engineering facility for the storage, use and 

disposal in which this organism is processed. 

iPS cells and/or GFP/RFP expressing cells produced by infection using viruses are deemed potential hazards 

and are graded Biosafety Level 1. 

Restrictions are placed on the distribution of Biosafety Level 2 cells. Cells infected with human pathogenic 

viruses, or known to release human pathogenic viruses may be distributed only to customers who provide 

evidence that they have the necessary authorization to work with pathogens. In Germany, a permit according 

to §44 Infektionsschutzgesetz (IfSG) must be provided. For exceptions, see §45 IfSG. Customers from outside 

Germany must show a valid permit provided by their competent authorities. PELOBiotech GmbH reserves the 

right to decline the shipment of Biosafety Level 2 cells. 

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GMP iPS cells 

GMP iPSCs are high-quality induced pluripotent stem cells derived from adult tissue biopsies, suitable for therapeutic 

applications. They meet strict regulatory standards to ensure their safety and effectiveness for human use. 

• We use StemRNA Reprogramming Technology, which is virus-free and complies with regulatory guidelines. 

• Benefits include iPSCs manufactured according to ICH 5QA standards, generated with footprint-free RNA 

reprogramming technology, and available for commercial use. 

• Diverse donors and over 30 years of experience in human tissue procurement. 

• StemRNA Reprogramming Technology produces robust iPSCs with low batch-to-batch variation, 

eliminating the need for screening exogenous genes. 

• Our iPSC seed stocks, Master Cell Banks, and working cell banks are suitable for commercial and therapeutic 

applications. 

iPS derived cells 

We use human iPSC technology, to create a wide range of cell models and biosensor technologies. Our capabilities cater 

to various applications, spanning preclinical drug discovery, biobanking, in vitro diagnostics, and biomarker 

development. 

Induced pluripotent stem (iPS) cells offer several advantages and serve as a superior human disease model compared to 

animal cells for several reasons: 

• Human Relevance: iPS cells are derived from human tissues, making them more relevant for studying human 

diseases. This is crucial because human physiology and disease mechanisms can differ significantly from those 

of animals. 

• Patient-Specific Modeling: iPS cells can be generated from individual patients, allowing the creation of patientspecific 

disease models. This is invaluable for studying genetic diseases and understanding the unique aspects 

of a patient's condition. 

• Disease Recapitulation: iPS cells can be differentiated into a variety of cell types relevant to the disease being 

studied, such as neurons, cardiomyocytes, or hepatocytes. This enables researchers to closely mimic disease 

conditions in a dish. 

• Genetic Manipulation: iPS cells can be genetically modified to introduce disease-associated mutations or correct 

genetic defects. This provides a precise way to investigate the genetic basis of diseases. 

• Drug Screening: iPS-derived cells can be used for high-throughput drug screening to identify potential therapies 

or test drug efficacy. This is particularly important for personalized medicine. 

• Reduced Ethical Concerns: Using iPS cells alleviates many ethical concerns associated with the use of embryonic 

stem cells, which can be controversial. 

• Consistency: iPS cells provide a consistent and reproducible source of human cells for experimentation, 

eliminating genetic variability found in animal models. 

• Translation to Clinical Applications: iPS cells have the potential to be used in cell-based therapies and 

regenerative medicine, making them a bridge between research and clinical applications. 

• Longitudinal Studies: Researchers can derive iPS cells from patients at different stages of a disease and track 

the disease progression over time, which is challenging to do with animal models. 

• Cost and Time Efficiency: iPS cell-based research is often more cost-effective and less time-consuming than 

working with animal models. 

The latest applications of induced pluripotent stem cells (iPSCs) encompass a wide array of cutting-edge advancements 

in regenerative medicine, disease modeling, drug screening, and cell therapy. These applications have been made possible 

by the unique properties of iPSCs, which are similar to embryonic stem cells (ESCs) in terms of morphology, proliferation, 

and gene expression profile (Okita & Yamanaka, 2008). iPSC technology has significantly enriched regenerative medicine 

by introducing autologous pluripotent progenitor pools bioengineered from ordinary somatic tissue, offering potential in 

disease modeling and therapeutic applications (Nelson et al., 2009; Polo et al., 2010). Notably, the first clinical study using 

human iPSC-derived cells was initiated in 2014, utilizing human iPSC-derived retinal pigment epithelial (RPE) cells to treat 

macular degeneration, resulting in improved vision for the patient (Shi et al., 2016). 

Recent advances in differentiating cells such as cardiac, neural, and skeletal muscle cells from iPSCs, as well as directly 

reprogramming somatic cells in tissue regeneration applications, have been summarized and synthesized, highlighting the 

versatility of iPSCs in various therapeutic contexts (Mao et al., 2022). iPSCs have also been explored for bone 

regeneration, cardiovascular disease, and as pre-clinical models for studying human disease, demonstrating their potential 

in diverse medical applications (He et al., 2018; Plews et al., 2012; Huang et al., 2020). 

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IPSCs have been investigated for their use in dentistry, immunotherapy, and as a tool for personalized autologous body 

organ transplant, showcasing their expanding role in different medical fields (Hynes et al., 2015; Jiang et al., 2013; Palomo 

et al., 2014). Moreover, iPSCs have been utilized for modeling inherited cardiomyopathies, bone regeneration, and as a 

platform for drug screening and disease modeling, further emphasizing their broad applications in medicine (Karakikes et 

al., 2014; Tang et al., 2014; Hwang et al., 2021). The potential of iPSCs in regenerative medicine has also been recognized 

through the development of liver buds and clinical-grade neural stem cells, highlighting the ongoing efforts to harness 

iPSCs for clinical applicability (Sekine et al., 2020; Cai et al., 2021). 

We offer: 

• Around 10,000 human disease models for preclinical drug development 

• Diverse models, including spheroid and organoid models, physiological barrier models, and more. 

• An extensive selection of iPSC derivative cell types, from pericytes to cardiomyocytes, for a wide range of 

research applications 

Below is a summary of the cell types we provide. Please note that this list is not exhaustive. To explore our complete cell 

offerings, we encourage you to request our detailed cell list or visit pelobiotech.com for additional information. 

iPSC-Derived Cardiomyocytes (Heart): 

Cardiotoxicity screening for drug development. 

Studying heart diseases and genetic disorders. 

Regenerative medicine for cardiac tissue repair. 

iPSC-Derived Hepatocytes (Liver): 

Drug metabolism and toxicity testing. 

Modeling liver diseases and viral infections. 

Development of bioartificial liver devices. 

iPSC-Derived Hepatic Stellate Cells and Kupffer Cells (Liver): 

Investigating liver fibrosis and cirrhosis. 

Testing anti-fibrotic drugs for liver disease treatment. 

Understanding hepatic stellate cell biology. 

iPSC-Derived Neurons (Brain): 

Modeling neurodegenerative diseases like Alzheimer's 

Drug discovery and testing for neurological disorders. 

Studying neural development and function. 

iPSC-Derived Keratinocytes (Skin): 

Skin disease modeling, such as epidermolysis bullosa. 

Wound healing and tissue regeneration studies. 

Drug testing for dermatological conditions. 

iPSC-Derived Endothelial Cells: 

Investigating cardiovascular diseases and vascular disorders. 

Testing anti-angiogenic drugs for cancer therapy. 

Vascular tissue engineering and regenerative medicine. 

iPSC-Derived Kidney Cells: 

Investigating renal diseases and disorders. 

Testing personalized medicaments for kidney health. 

Studying drug responses specific to kidney function 

iPSC-Derived Hematopoietic Stem Cells (HSCs): 

Studying blood disorders like anemia and leukemia. 

Personalized therapies for hematological disorders 

Drug screening for hematological conditions. 

iPSC-Derived Hematopoietic Stem Cells (HSCs): 

Studying blood disorders like anemia and leukemia. 

Developing personalized cell therapies 

for blood-related diseases. 

Drug screening for hematological conditions. 

iPSC-Derived Immune Cells: 

Modeling autoimmune diseases and immunodeficiencies. 

Developing immunotherapies and vaccines. 

Screening for immunomodulatory drugs and treatments. 

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Adult Stem Cells 

Adult stem cells, also known as somatic or multipotent stem cells, are a unique class of undifferentiated cells found within 

various tissues and organs of the human body. Unlike embryonic stem cells, which can differentiate into any cell type, 

adult stem cells are more restricted in their potential but still possess the ability to develop into a range of specialized cell 

types within the specific tissue or organ where they reside as shown in the figure above. However, these cells play a vital 

role in tissue maintenance. As you can see in the figure they form cells that repair, and aid in regeneration throughout an 

individual's life, making them a valuable resource for regenerative medicine and research aimed at understanding and 

treating various medical conditions. We offer the following cells: 

• Hematopoietic CD34+ stem cells and Mononuclear cells to isolate CD34 or CD113 cells 

• Available from both males and females, complete with donor clinical status. 

• Sourced from peripheral blood, cord blood and bone marrow. 

CD34 is a crucial marker for various progenitor cells, particularly hematopoietic stem cells (HSC) and hematopoietic 

progenitor cells (Sidney et al., 2014). It plays a significant role in hematopoiesis, as evidenced by its presence in progenitor 

cells during hematopoiesis, and its potential to give rise to hematopoietic, endothelial cells, and smooth muscle cells 

(Kapoor et al., 2019; Bai et al., 2009). CD34 has been linked to cellular homing patterns to the bone marrow and adhesion 

of cells to stromal microenvironments or sites of inflammation (Kopher et al., 2010). Furthermore, CD34(+) stem cells 

have been found to play an important role during liver development and regeneration (Park et al., 2015). Additionally, 

CD34 has been associated with the regulation and compartmentalization of stem cells, promoting their adhesive 

interactions with the stromal microenvironment of the bone marrow (Healy et al., 1995). It has also been identified as a 

marker for hematopoietic stem cells and non-hematopoietic progenitors, including vascular endothelial progenitors and 

embryonic fibroblasts, multipotent mesenchymal stromal cells, interstitial dendritic cells, and epithelial progenitors 

(Soliman, 2021). Moreover, CD34 has been utilized as an important marker for labeling hematopoietic stem cells, 

demonstrating its significance in stem cell research and applications (Ye et al., 2004). 

Mesenchymal/Stromal Stem cells 

Mesenchymal stromal cells (MSCs), often called mesenchymal stem cells, are adult stem cells found in bone marrow, 

adipose, Wharton jelly and other tissues. Notable characteristics include: 

• Differentiation into various cell types, such as bone, cartilage, and fat cells. 

• Immune system modulation, making them valuable for treating autoimmune diseases, inflammatory conditions, 

and tissue damage. 

• Regenerative properties that promote tissue repair. 

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• Low immunogenicity, reducing the risk of immune rejection when transplanted. 

Additionally, we offer a wide range of MSCs in research and GMP grade from various tissue sources, including canines, 

monkeys, dogs, mice, and humans, with different characteristics and applications, such as diseased models, 

conditionally immortalized cells, and those with GFP/RFP tags and cell RNA. For all of these MSCs we also offer 

corresponding media and tools. 

GMP MSCs 

We provide high-quality mesenchymal stromal cells (MSCs) for clinical and preclinical applications, offering 

both GMP and research-grade options. These cells are derived from 20 years of research and clinical experience 

at the Karolinska Institute, ensuring their quality. Our large-scale facility in Stockholm enables cost-effective 

production. 

• Adjustable quantity and vial size per dose. 

• Derived from bone marrow or adipose tissue of young, healthy volunteers. 

• Xeno-free and at Passage 3. 

• Offered with international delivery in liquid nitrogen. 

Exosomes 

These versatile cells cater to various research and clinical needs, with competitive pricing and delivery options 

for different requirements. 

Exosomes, small extracellular vesicles released by various cell types, have garnered significant attention for their diverse 

applications in both diagnostics and therapeutics. These tiny membrane-bound vesicles carry a cargo of proteins, nucleic 

acids, and lipids, facilitating intercellular communication. In diagnostics, exosomes serve as potential biomarkers for 

various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions. Their non-invasive nature 

and specificity make them valuable in early disease detection. Moreover, exosomes hold promise in therapeutics, acting 

as natural delivery vehicles for drugs, RNA, or therapeutic proteins. Their ability to cross biological barriers, such as the 

blood-brain barrier, enhances their potential for targeted drug delivery. As research continues to unveil the intricate roles 

of exosomes, their applications are expanding, offering innovative solutions in personalized medicine and regenerative 

therapies. 

We offer exosomes produced from bone marrow mesenchymal stem cells which are CD9, CD63 and CD81 +ve, 

extensively tested and size and count verified by Zetaview. 

Also available are media (CellCor) and media supplements for exosome production using mesenchymal stem/stroma 

cells (see page 29-30). 

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Primary Cells 

In the realm of applied research, the selection of cell cultures is a critical determinant of 

the quality and relevance of scientific outcomes. Our commitment to maintaining the 

highest standards in cell quality has resulted in one of the most extensive portfolios of 

primary cells in Europe. To ensure the excellence of our cells, they undergo a rigorous 

process before they reach you. We also offer corresponding media for all the cells or 

custom media tailored to your requirements and all other possible cell culture tools. 

• Tissue Selection: Our meticulous tissue selection guarantees the highest 

quality primary cells, enhancing research reliability and are generally available 

at a cell density >0.5 million cells per vial check on our webpage for details. 

• Stringent Testing: We subject every isolation to comprehensive testing, 

including population doubling time, proliferation assays, and tests for specific 

markers, fungi, bacteria, mycoplasma, hepatitis B and C DNA, and HIV-1 DNA. 

• Donor Information: Certain cell types come with extensive donor data, 

enriching research with details like BMI, smoking history, gender, and medical 

history. 

• Species Diversity: Our extensive cell range spans mice, rats, hamsters, chickens, 

pigs, cats, dogs, cattle, and humans. Human cells can also be acquired from 

diseased or elderly donor sources, broadening research possibilities. 

• Quality Control: Automated liquid nitrogen control ensures consistent, 

high-quality cryopreserved cell batches, maintaining our stringent standards. 

Additional Services we provide: 

• Custom Cell Isolation: Researchers with specific requirements can take advantage of our custom cell isolation 

services. We offer the flexibility to work with general protocols or, if preferred, the researcher's own protocols, 

all at affordable prices. 

• Cell RNA: We offer cell RNA, providing a valuable resource for RNA sequencing work. 

• Tagged Cells: We provide cells tagged with markers such as GFP/RFP, facilitating tracking and visualization in 

experiments. 

• Immortalized Lines: Our selection includes immortalized cells, which can be advantageous for extended and 

consistent experimentation. 

• Cells from KO Mice: We offer cells from specific knockout (KO) mice, enabling researchers to explore the 

effects of specific gene disruptions. 

• Media and other cell culture tools: we offer corresponding media for all the cells that we offer and various 

tools to aid your cell culture from coatings, supplements to bioreactors. 

Our commitment to providing top-tier primary cells and related services underscores our dedication to advancing the 

quality and relevance of your research endeavors. 

Heart 

In our diverse cell portfolio, we provide over 900 cell types. Apart from cardiomyocytes and iPS derived cardiomyocytes, 

we also isolate endothelial cells, preadipocytes, myocytes, epithelial cells, and fibroblasts from different regions of the 

heart and from various species. A spectrum of heart-derived cell types, each with distinct characteristics, contributes to 

diverse realms of cardiovascular research. Cardiomyocytes, the heart's muscle cells, find applications in drug screening, 

cardiac toxicity assessment, and investigations into cardiac hypertrophy and regeneration. Endothelial cells, lining 

blood vessels, play a pivotal role in studies related to angiogenesis, atherosclerosis, and vascular inflammation. 

Preadipocytes, precursors to adipocytes, are integral in research focusing on adipogenesis, obesity-related conditions, 

and metabolic disorders. 

Myocytes, whether cardiac or skeletal, are fundamental in deciphering heart function, arrhythmias, and muscular disorders. 

Epithelial cells, forming the outer layers of the heart and blood vessels, contribute to studies on tissue development, 

regeneration, and barrier function. Finally, fibroblasts, providing structural support, are indispensable in exploring fibrosis, 

extracellular matrix dynamics, and tissue repair mechanisms. This diverse array of heart-derived cells collectively propels 

advancements in cardiac biology, offering insights into development, function, and pathology, with applications spanning 

from basic research to therapeutic development and regenerative medicine. 

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Scan the QR code to see our full cell list.


You can also check out our Heart slices for toxicology, arrythmia related work which is more translationally relevant. You 

can read more about the use of heart slices for research in this article here. 

Brain 

Apart from isolated and iPS derived astrocytes, Schwann cell like cells, microglia, oligodendrocytes, sensory neurons, 

cortical neurons, dopamergic neurons we also isolate endothelial 

cells, glioblastomas, pericytes, neuron precursor cells from different 

regions of the heart and from various species. Isolated and induced 

pluripotent stem (iPS) cell-derived neural cell types, such as 

astrocytes, Schwann cell-like cells, microglia, oligodendrocytes, 

sensory neurons, cortical neurons, and dopaminergic neurons, serve 

as invaluable tools across a spectrum of studies and applications. 

Astrocytes, Schwann cell-like cells, microglia, and oligodendrocytes 

play crucial roles in various aspects of neuroscience research. 

Astrocytes, for instance, are involved in the regulation of cerebral blood 

flow, maintenance of synaptic environments, and modulation of 

synapse formation and synaptic transmission. They are also implicated in the glymphatic system's ability to eliminate waste 

in the brain and are essential for the maintenance of brain homeostasis and neuronal protection. Furthermore, astrocytes 

are involved in regulating neuronal synaptogenesis, maintaining blood-brain barrier integrity, and recycling 

neurotransmitters. Additionally, they have been shown to mediate analogous memory in a multi-layer neuron-astrocyte 

network, and targeting astrocytic function may protect against brain injury induced by blood-brain barrier disruption. 

Schwann cell-like cells have been studied for their morphological, transcriptional, and functional differences from mouse 

astrocytes, and they play key roles in supporting the central nervous system structure, regulating synaptic functions, and 

maintaining brain homeostasis. Microglia, on the other hand, have been found to regulate synapses, neuronal circuits, and 

behavior, and they are thought to play a pivotal role in coupling neural activity and cerebral blood flow. Oligodendrocytes, 

which are responsible for producing myelin in the central nervous system, have been implicated in the regulation of neuronal 

excitability, synaptic transmission, plasticity, and in higher cognitive functions, including the initiation, maintenance, and 

consolidation of memories. 

Additionally, isolated endothelial cells, glioblastomas, pericytes, and neuron precursor cells from various brain regions 

offer insights into angiogenesis, brain tumors, blood-brain barrier function, and neurogenesis, respectively. These diverse 

neural cell types facilitate a broad range of studies, including disease modeling, drug screening, neurotoxicity testing, and 

elucidating fundamental mechanisms underlying neurological and neurodegenerative disorders. 

We also offer brain tissue slices Brain slices, including brain and root ganglionic slices, are valuable tools in neuroscience 

research due to their ability to provide a controlled and accessible model for studying various aspects of neuroscience. 

Brain slices have been widely used to study neuronal differentiation, cell differentiation, and synaptic transmission. They 

have also been utilized to model neurodegenerative proteinopathies and to enhance the quality of acute slice preparations, 

particularly for electrophysiology studies. 

Brain slices have been instrumental in profiling sequential sections with multiplex staining, which benefits research in life 

sciences. Furthermore, brain slice preparations have been validated for enhancing neuronal preservation and overall brain 

slice viability, making them essential for studying metabolic changes and facilitating experimentation on age-related 

disorders. Moreover, root ganglionic slices, such as dorsal root ganglion neurons, have been used to study neuronal 

connectivity, synaptic transmission, and plasticity, as well as to perform whole-cell patch-clamp recordings, allowing 

precise measurement of cellular and synaptic properties. 

The dorsal root ganglion has been identified as a novel neuromodulatory target for evoking strong and reproducible 

motor responses in chronic motor complete spinal cord injury. Studies have been conducted on the trigeminal ganglion 

morphology in human fetuses, and the trigeminal ganglion has been studied for its potential in treating trigeminal 

neuralgia. 

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Pancreas 

We extract beta cells and diseased cells from diabetic 

patients from the pancreas. Additionally, we isolate 

endothelial cells, epithelial cells, fibroblasts, and PDX 

cancer cells from different pancreatic regions and various 

species. The uses of beta cells in research are extensive 

and diverse. Studies have focused on understanding the 

factors influencing beta cell turnover, proliferation, and 

functional state, particularly in the context of diabetes and 

regenerative therapy. 

Research has explored the essential role of Nkx6.1 in 

maintaining the functional state of pancreatic beta cells, 

adaptive changes in beta cell turnover during pregnancy, 

the relationship between beta-cell mass and obesity/type 2 

diabetes, and the gene regulatory network required for 

establishing and maintaining pancreatic beta cell identity. 

Additionally, the beta cell workload hypothesis has been 

revisited in the context of type 2 diabetes, and 

mathematical modeling has been used to estimate the long lifespan and low turnover of human islet beta cells. 

Studies have investigated the proliferation of sorted human and rat beta cells, targeted insulin-producing beta cells for 

regenerative therapy, and explored the potential for beta cell regeneration in aging pancreatic beta cells. Single-cell RNA 

sequencing has revealed a role for reactive oxygen species and peroxiredoxins in fatty acid-induced rat beta-cell 

proliferation, and reciprocal modulation of adult beta cell maturity by activin A and follistatin has been studied. Moreover, 

research has focused on beta cell regeneration after immunological destruction in a mouse model, explored the potential 

for making better beta cells, and investigated agents inducing both alpha and beta cell proliferation without affecting 

differentiation or viability. Additionally, efforts have been made to expand human beta cells, and heterogeneities of normal 

and stimulated pancreatic beta cells have been examined. These studies collectively contribute to a comprehensive 

understanding of beta cell biology and its implications for diabetes and regenerative medicine. 

Lungs and Oral cavity 

Apart from isolated and iPS derived alveolar and 

bronchial epithelial cells, fibroblasts, smooth muscle 

cells, macrophages, endothelial cells PDX cancer cells, 

mesenchymal stem cells from different regions of the 

heart and various species. The uses of iPS-derived 

alveolar and bronchial epithelial cells, fibroblasts, 

smooth muscle cells, macrophages, endothelial cells, 

PDX cancer cells, and mesenchymal stem cells from 

different regions of the heart and various species are 

diverse and impactful in biomedical research. Patientderived 

xenograft (PDX) models have been extensively 

used in cancer research to simulate human tumor 

biology in vivo, aiding in the development of anticancer 

drugs and providing a better representation of tumor 

heterogeneity and the tumor microenvironment. 

Mesenchymal stem cells (MSCs) have shown promise in the treatment of chronic lung diseases and ischemic heart 

disease, with studies demonstrating their potential for myocardial survival and repair, as well as their ability to 

differentiate into endothelial cells, vascular smooth muscle cells, or cardiac-like myocytes when transplanted into the 

ischemic heart. Smooth muscle cells have been studied for their contractile properties and their association with neuroglial 

cells and interstitial cells of Cajal in intestinal tissue engineering. Additionally, endothelial and smooth muscle cells have 

been isolated and characterized from coronary vessels, providing insights into their contractile phenotype and potential 

applications in vascular research. 

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Furthermore, the establishment of living biobanks comprising patient-derived xenografts, primary tumor tissue, normal 

tissue, sera, and cancer cell lines has facilitated the study of tumor characteristics and mechanisms of atherosclerosis. The 

expression of PDX-1, a key regulator of beta cell maturation and differentiation, has been investigated in pancreatic 

cancer, shedding light on its significance in the disease. Moreover, the potential of stem cells in the treatment of skeletal 

muscle injury and disease has been explored, highlighting the therapeutic implications of multipotent stem cells derived 

from adult bone marrow, fat, skin, periosteum, and muscle. These studies collectively demonstrate the wide-ranging 

applications of these cell types in advancing our understanding of various diseases and in the development of novel 

therapeutic strategies. 

Kidney 

We offer a range of fibroblasts, endothelial cells, epithelial 

cells, mesangial cells, and podocytes from various regions of 

the kidney, sourced from different species. Fibroblasts play a 

crucial role in renal fibrosis, and their origin in renal fibrosis has 

been a subject of controversy, highlighting the importance of 

understanding their role in kidney pathology. Endothelial cells 

have been studied for their involvement in angiogenesis and 

their response to various factors in the context of kidney 

diseases, providing insights into their role in kidney function and 

pathology. Epithelial cells have been investigated for their 

protective role against oxidative cell injury, shedding light on 

their significance in acute kidney injury and chronic kidney 

diseases. 

Mesangial cells have been shown to participate in immune function in the kidney and have been studied for their role in 

maintaining the structure and function of the glomerulus and in the pathogenesis of glomerular diseases. Podocytes have 

been studied for their involvement in renal fibrosis and their response to growth factors, providing insights into their role 

in kidney fibrosis and compensatory hypertrophy. The study of bone marrow-derived cells carrying a polycystic kidney 

disease mutation in the genetically normal kidney has provided valuable insights into the fate of these cells in the kidney, 

particularly following renal injury, contributing to our understanding of kidney regeneration and disease progression. 

Additionally, the expression of a novel mesangium-predominant gene, Megsin, in the renal tissues of various glomerular 

diseases has been investigated, shedding light on its potential role in the pathogenesis of kidney diseases. Moreover, the 

use of laser ablation of the zebrafish pronephros to study renal epithelial regeneration has provided a novel tool for the 

study of epithelial injury in zebrafish, offering insights into kidney regeneration and repair. These studies collectively 

contribute to a comprehensive understanding of kidney biology and pathology, providing a basis for the development of 

novel therapeutic strategies for kidney diseases. 

Intestine 

Smooth muscle cells have been investigated for their 

involvement in intestinal motility, peristalsis, and the 

pathogenesis of intestinal fibrosis. Studies have shown that 

smooth muscle cells play a crucial role in the regulation of 

intestinal motility and peristalsis, and their phenotypic 

plasticity has been implicated in mechanical obstruction of the 

small intestine. Smooth muscle cells have been used in tissue 

engineering for intestinal reconstruction, exhibiting 

spontaneous rhythmic contraction in vitro and showing 

potential for functional smooth muscle bioengineering. 

Fibroblasts have been studied for their involvement in 

intestinal fibrosis, inflammation, and the regulation of 

intestinal epithelial cells. Studies have shown that fibroblasts 

play a role in the pathogenesis of intestinal strictures in Crohn's 

disease and have been implicated in the secretion of inflammatory mediators from LPS-induced rat intestinal microvascular 

endothelial cells. Additionally, fibroblasts have been shown to modulate colonocytes in co-culture and contribute to the 

development of intestinal fibrosis. Endothelial cells have been extensively studied for their involvement in intestinal 

microvascular development, angiogenesis, and the regulation of intestinal fibrosis. Studies have shown that endothelial 

cells express Toll-like receptor 5 and are involved in the secretion of inflammatory mediators from LPS-induced rat 

intestinal microvascular endothelial cells. 

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Additionally, endothelial cells have been implicated in the development of intestinal fibrosis and have been shown to 

contribute to the pathogenesis of necrotizing enterocolitis. Epithelial cells have been investigated for their involvement 

in intestinal inflammation, barrier function, and the response to radiation-induced injury. Studies have shown that 

epithelial cells are involved in radiation-induced intestinal injury, with the epithelial cells being the most important target 

of radiation damage, which may subsequently induce apoptosis in endothelial cells. 

Scan the QR code to see our full cell list. 

Urethra 

We provide primarily epithelial cells from the urethra, 

and the RNA of these cells as well. The primarily 

epithelial cells from the urethra have been studied for 

their role in the development, function, and 

regeneration of the urethra. These cells have been 

shown to express androgen receptors, and their 

differentiation has been observed in the urothelium 

of the prostatic urethra and prostatic luminal 

epithelial cells. Single-cell RNA sequencing has been 

used to obtain a complete transcriptomic profile of all 

epithelial cells in the mouse prostate and urethra to 

identify cellular subtypes. The expression of 

cytokeratin 7, uroplakin, and FoxA1 has been 

observed in the urethral plate and developing urethra 

of the penile glans, indicating the presence of 

proteins of epithelial cells of endodermal origin. 

The urethral luminal epithelia have been identified as castration-insensitive cells of the proximal prostate, and they have 

been shown to undergo a prostate luminal to club cell transition in benign prostatic hyperplasia. Additionally, the urethral 

epithelial cells have been used in the fabrication of tissue-engineered bionic urethras using cell sheet technology for fullthickness 

urethral reconstruction. These studies collectively demonstrate the diverse applications of primarily epithelial 

cells from the urethra in understanding urethral development, function, and regeneration. 

Stomach 

We extract epithelial cells, smooth muscle cells, and primary stomach cells from the stomach, sourcing them from 

various species. Epithelial cells from the stomach have been investigated for their role in the development and function 

of the stomach. Studies have shown that the completeness of the stomach development is marked by the appearance of 

non-specific esterase activity in the stomach epithelial cells. Additionally, the adherence of lactic acid bacteria to the 

columnar epithelial cells of the stomach has been studied, providing insights into the microbial colonization of the 

stomach. Smooth muscle cells from the stomach have been studied for their involvement in gastric motility and the 

pathogenesis of stomach diseases. These cells play a crucial role in the regulation of gastric motility and peristalsis, 

contributing to the digestive function of the stomach. 

Umbilical cord 

We isolate a range of cell types, including human umbilical vein endothelial cells, 

various shRNA-stable transfected HUVECs, HUVECs with GFP tags in the 

mitochondria and plasma membrane, conditionally immortalized umbilical cord 

MSCs, HUVECs from arteries and veins, and whole cell RNAs. 

The uses of human umbilical vein endothelial cells (HUVECs) are diverse and 

significant in various research areas. HUVECs have been utilized in studying 

cytoprotective pathways, modeling atherosclerosis, investigating protective effects 

against injury, analyzing angiogenesis and lymphangiogenesis, and exploring 

autophagy induction. HUVECs have been employed in the study of lactateinduced 

pathways in endothelial cells, as well as in investigating the effects of 

activated protein C on endoplasmic reticulum stress and apoptosis. 

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HUVECs have been used in the cultivation of cardiovascular grafts to study fibrinolytic parameters. The versatility of 

HUVECs is evident in their application in various fields such as medicine, biology, and endothelial dysfunction research. 

HUVECs have also been employed in the investigation of signaling pathways, including the lactate/NFkB/IL-8 pathway, 

which plays a crucial role in tumor metabolism and angiogenesis. HUVECs have been utilized to study the interaction 

between TRPV4 and KCa2.3 in the context of hypertension treatment. 

The significance of HUVECs in research is further emphasized by their use in the study of autophagy, apoptosis, and 

oxidative stress, as well as their role in modeling atherosclerosis and investigating protective mechanisms against injury. 

GFP-tagged with liposomes and viruses HUVECs offer real-time visualization of subcellular structures, facilitating studies 

on cellular dynamics and interactions. Conditionally immortalized umbilical cord MSCs are valuable for regenerative 

medicine research, exploring their potential in tissue engineering and cell-based therapies. 

Breast 

We isolate epithelial cells, fibroblasts, endothelial cells, breast cancer cells from tumors, as well as patient-derived 

xenograft (PDX) cells, and breast cancer-associated fibroblasts from breast tissue. These samples are sourced from 

various species. The use of epithelial cells, fibroblasts, endothelial cells, breast cancer cells from tumors, patient-derived 

xenograft (PDX) cells, and breast cancer-associated fibroblasts from breast tissue are diverse and significant in various 

research and clinical applications. Epithelial cells have been utilized in the separation of breast cancer cells from peripherally 

circulating blood, particularly through the use of antibodies fixed in microchannels, which enables the isolation of breast 

cancer cells at an early stage of tumor growth and metastasis. 

Fibroblasts have been employed in regenerative medicine due to their potential to provide appropriate cross-talk and 

extracellular matrix (ECM) production to maintain tissue homeostasis and enable repair. Additionally, fibroblasts have 

been used in the treatment of skin disorders, such as recessive dystrophic epidermolysis bullosa, and have been applied 

as a biological dressing in wound beds. Furthermore, fibroblasts have been studied for their paracrine anti-fibrotic effects 

on young and senescent human dermal fibroblasts, indicating their potential in wound repair and regeneration. 

Breast cancer cells from tumors have been utilized in various studies, including the investigation of cancer-associated 

fibroblasts (CAFs) inducing epithelial–mesenchymal transition (EMT) of breast cancer cells through paracrine TGF-β 

signaling, and the promotion of breast cancer cell malignancy via CXCL5 secretion in the tumor microenvironment. 

Additionally, patient-derived tumor cells, combined with three-dimensional culture technology, have been used to form 

breast cancer organoids that resemble the in vivo tumor structure. Furthermore, patient-derived xenograft (PDX) cells 

have been employed in studying the progression of breast cancer through the regulation of epithelial and stromal cell 

signaling in the TGF-β pathway. Breast cancer-associated fibroblasts from breast tissue have been studied for their role in 

promoting breast tumorigenesis through the secretion of hepatocyte growth factor and the expansion of cancer-stem like 

cells. Additionally, engineered human breast tissue models have been used to show that ATR-deficient breast stromal 

fibroblasts enhance the growth of breast cancer cells. 

Ovaries 

We isolate epithelial cells, fibroblasts, endothelial cells, smooth muscle cells, and ovarian cancer cells from tumors, in 

addition to patient-derived xenograft (PDX) cells and ovarian cancer-associated fibroblasts from ovarian tissue. These 

samples are sourced from various species. Epithelial cells are crucial for studying tissue development and differentiation, 

while fibroblasts play a pivotal role in extracellular matrix synthesis and tissue remodeling. Endothelial cells contribute to 

angiogenesis and vascular biology, and smooth muscle cells are integral in understanding vascular and muscular 

physiology. Isolating ovarian cancer cells from tumors facilitates studies on cancer progression and potential therapeutic 

targets. PDX cells, derived from patient tumors and engrafted into immunocompromised mice, serve as powerful models 

for preclinical drug testing, allowing researchers to assess treatment efficacy and tumor response in a more clinically 

relevant context. Ovarian cancer-associated fibroblasts derived from ovarian tissues contribute to the understanding of 

tumor microenvironment interactions. 

Testes and prostate 

We extract epithelial cells, fibroblasts, endothelial cells, smooth muscle cells, and prostate cancer cells from tumors, in 

addition to patient-derived xenograft (PDX) cells and prostate cancer-associated fibroblasts from prostate tissue. These 

samples are sourced from various species. Additionally, total RNA from the prostate and testes is also available. Epithelial 

cells are vital for studying tissue structure and function, often applied in investigations related to epithelial biology, wound 

healing, and drug response. 

Fibroblasts have been employed in the regulation of biomechanical properties of human microvascular endothelial cells, 

indicating their significance in cancer cell interactions and the tumor microenvironment. Fibroblasts have been studied for 

their potential role in the regulation of the immune and epithelial barrier responses in the prostate. Endothelial cells play 

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an essential role in vessel homeostasis, and their comprehensive phenotyping using flow cytometry is critical in vascular 

biology research. Interactions between endothelial cells and electrospun fibers have been studied for engineered vascular 

replacements, highlighting the importance of endothelial cell orientation along blood flow to increase their ability to resist 

shear stress. Smooth muscle cells from the testes and prostate have been less commonly studied in the provided 

references, and their specific uses in research and clinical applications are not as extensively documented. However, their 

potential role in the physiology and pathology of the testes and prostate may include contributions to the understanding 

of male reproductive health and disorders related to smooth muscle function in these organs. Prostate cancer cells from 

tumors, PDX cells, and prostate cancer-associated fibroblasts are invaluable tools for prostate cancer research, aiding in 

drug development, biomarker discovery, and understanding tumor-stroma interactions. 

Cells derived from the liver 

In our extensive cell portfolio, we offer over 900 cells. In addition to hepatocytes, we isolate endothelial cells, epithelial 

cells, and fibroblasts from various liver regions listed below, we also have Kupffer cells, stellate cells, Hematopoietic stem 

cells, and non-parenchymal cells. Endothelial cells are pivotal in studies related to angiogenesis, vascular biology, and 

drug delivery, offering insights into liver-specific vascular responses. Epithelial cells play a crucial role in understanding 

liver tissue regeneration, differentiation, and responses to injury or disease. 

Hepatocytes 

Discover our premium Hepatocytes for Drug Testing: Hepatocytes are the ideal choice for your short- and long-term drug 

screening and testing needs, offering several key advantages: 

1. Comprehensive Testing: 

Our hepatocytes are extensively tested for vital cytochrome P450 (CYP) enzymes, including CYP1A2, CYP2B6, 

CYP2C8, CYP2C9, and CYP2C19. We also assess Phase I and Phase II metabolism as well as transporter 

profiling, ensuring their functionality and reliability for your experiments. 

2. Ample Supply: 

We provide hepatocytes in generous lot sizes, delivering 5-10 million viable cells per vial, ensuring you have 

a consistent and plentiful source of cells for your research. 

3. High Cell Viability: 

Our hepatocytes consistently achieve over 90% cell viability, guaranteeing robust and dependable results for 

your experiments. 

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4. Detailed Donor Information: 

We offer in-depth donor information, including gender, age, ethnicity, BMI, cause of death, smoking and 

alcohol history, HLA typing, serology data, and past medical records, allowing you to choose cells that align 

with your specific research requirements. 

5. Versatility in Testing: 

Our hepatocytes are not only plateable but also 3D tested, offering versatility for various research applications, 

from monolayer cultures to complex 3D experiments. Please contact us for detailed information about 3D cell 

culture to find out how we can help you better. 

Explore the intricate and vital role of hepatocytes in the human body's metabolism of xenobiotics and 

drugs by reading our article on our News section on website for in-depth information. Just scan the QR 

code for direct access. 


Fibroblasts contribute to studies on liver fibrosis and tissue repair, shedding light on the cellular dynamics in hepatic 

wound healing. Kupffer cells, as liver-resident macrophages, are key players in immune response studies, inflammation, 

and liver pathophysiology. Stellate cells, involved in liver fibrosis, are integral for investigations into hepatic stellate cell 

activation and its impact on liver health. 

Hematopoietic stem cells extracted from the liver provide a unique perspective on hematopoiesis and immune cell 

development. Non-parenchymal cells, comprising various liver cell types, enable comprehensive studies encompassing 

liver microenvironment, cell-to-cell interactions, and organ-specific responses to therapeutic interventions. 

Stellate cells, also known as hepatic stellate cells, play a significant role in liver fibrosis, and their activation is a key event 

in the development of fibrosis. They are involved in the resolution of liver injury and are the primary storage site for 

retinoids in the liver. Additionally, stellate cells have been shown to express desmin, suggesting their contribution to the 

stellate cell population. Furthermore, stellate cells have been implicated in the regulation of liver fibrosis and have been 

shown to produce increased levels of inflammatory mediators, contributing to the fibrogenic program of liver cells. They 

are also involved in the turnover of extracellular matrix, and their activation is a major pathogenic determinant of liver 

fibrosis. Moreover, stellate cells have been shown to be coupled to one another by gap junctions, indicating their role in 

intercellular communication. Additionally, stellate cells have been studied in the context of liver regeneration, and their 

inhibition has been shown to reduce renal fibrosis. 

Kupffer cells, which are liver-resident macrophages, play a crucial role in liver homeostasis, inflammation, and immune 

regulation. They have been implicated in the pathogenesis of wound healing and fibrosis in the liver. Kupffer cells have 

been shown to be involved in the regulation of stellate cell activation and susceptibility to fibrotic liver disease. 

Sinusoidal microvascular endothelial cells, which line the hepatic sinusoids, have been studied for their involvement in 

liver fibrogenesis and the resolution of liver injury. They have been shown to be regulated by vitamin A and retinoic acid 

signaling, which are essential for their development and liver morphogenesis. Endothelin antagonism has been studied in 

the context of hepatic fibrosis, implicating endothelial cells in the pathogenesis of wound healing and fibrosis. 

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Eyes 

We isolate retinal pigment cells, endothelial cells, cryopreserved 

CnCePc, RbCEpCs, keratinocytes, meshwork cells, retinal 

astrocytes, and various immortalized pericytes from the eyes. 

These samples are sourced from various species and various 

regions and vessels of the eye. Retinal pigment epithelial (RPE) 

cells play a crucial role in maintaining retinal homeostasis and 

visual function. They have been extensively studied for their 

involvement in retinal diseases, such as age-related macular 

degeneration (AMD), and have been used to model retinal 

pathologies in vitro. RPE cells have been shown to be involved in 

the regulation of the blood-retinal barrier (BRB) and have been 

used to establish cell lines and study transport functions across the 

inner BRB. Additionally, RPE cells have been implicated in the 

transfer of melanosomes to keratinocytes, contributing to skin 

pigmentation and melanosome distribution. Furthermore, RPE 

cells have been studied for their involvement in the pathogenesis 

of diabetic retinopathy and have been used to establish a human 

retinal pericyte line, providing a novel tool for the study of diabetic 

retinopathy. 

Endothelial cells have been extensively studied for their role in angiogenesis, blood-brain barrier (BBB) function, and 

the regulation of vascular permeability. They have been used to establish cell lines and study the formation and disruption 

of the BBB, providing insights into CNS homeostasis and the pathogenesis of neurological disorders. Endothelial cells have 

been implicated in the regulation of the blood-nerve barrier (BNB) and have been used to establish a new in vitro BNB 

model, contributing to the study of peripheral neuropathy and peripheral nerve function. Cryopreserved corneal 

endothelial precursor cells (CnCePc) and retinal endothelial cells (RbCEpCs) have been studied for their potential in corneal 

and retinal tissue engineering and regenerative medicine. They have been used to establish cell lines and study the 

expression of wound-healing-related genes in primary human keratinocytes from burn patients, providing insights into 

the potential therapeutic applications of these cells in wound healing and tissue regeneration. Keratinocytes have been 

extensively studied for their role in skin pigmentation, wound healing, and skin regenerative applications. 

They have been used to study melanosome transfer, phagocytic activity, and the distribution pattern of melanosomes, 

shedding light on their role in skin pigmentation and melanosome transfer to keratinocytes. Keratinocytes have been used 

to establish a new immortalized human lung pericyte cell line, providing a promising tool for human lung pericyte studies 

and contributing to the study of lung pericyte functions in vitro. Meshwork cells, also known as trabecular meshwork, 

have been studied for their involvement in glaucoma and aqueous humor outflow regulation. They have been used to 

study the regulation of extracellular-superoxide dismutase and have been implicated in the regulation of L-arginine 

transport in retinal pericytes, providing insights into their role in retinal pericyte function and oxidative stress regulation. 

Retinal astrocytes have been studied for their involvement in neuroinflammation and the blood-brain barrier (BBB). 

They have been used to identify distinct contributions of astrocytes and pericytes to neuroinflammation and have been 

implicated in the regulation of the blood-brain barrier (BBB) and the maintenance of CNS homeostasis. Immortalized 

pericytes have been extensively studied for their involvement in the blood-brain barrier (BBB), retinal pericyte function, 

and the regulation of L-arginine transport. They have been used to study the expression of rat ABCG2 on the luminal side 

of brain capillaries and have been implicated in the regulation of L-arginine transport in retinal pericytes, providing insights 

into their role in retinal pericyte function and the blood-brain barrier (BBB). 

Lymphatic system 

We provide fibroblasts and endothelial cells from various vessels of the lymphatic system in different animal species. 

Fibroblasts have been studied for their potential role in promoting lymphangiogenesis in head and neck neoplasms, 

providing insight into the pathways leading to lymphatic vessel formation in each patient. Fibroblasts surrounding cystic 

lymphatics have been found to induce the expression of Amphiregulin in lymphatic endothelial cells, suggesting their 

involvement in the pathogenesis of cystic lymphangioma. Cancer-associated fibroblasts have been shown to promote 

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lymphangiogenesis in a lymphatic organotypic co-culture model, providing valuable insights into the mechanisms 

underlying lymphatic vessel formation in the context of tumor microenvironment. Endothelial cells from the lymphatic 

system have been extensively studied for their role in lymphangiogenesis and the development of the lymphatic 

vasculature. 

Studies have demonstrated that Prox1 is a master control gene in the program specifying lymphatic endothelial cell fate, 

highlighting its essential role in the induction of the lymphatic endothelial cell phenotype. The expression of podoplanin 

in stromal fibroblasts has been associated with high lymphatic vessel density in triple-negative, basal marker-expressing, 

and high-grade breast carcinomas, indicating its potential as a marker for lymphatic endothelial cells and 

lymphangiogenesis. The differential response of lymphatic endothelial cells to angiopoietin-1 and angiopoietin-2 has 

been investigated, providing insights into the molecular regulation of lymphangiogenesis and the distinct functions of 

angiopoietins in lymphatic vessel development. 

Skeletal system 

We offer a variety of synovial cells, chondrocytes, osteoblasts, endothelial cells from various vessels, and tenocytes 

sourced from different animal species, with cell RNA from adults and fetuses all of which are extracted from various 

bones. Synovial cells have been studied for their modulatory effect on cell phenotype and metabolic behavior in 

osteoarthritic patients, providing insights into the inflammatory and metabolomic profile of synovial fluid and its impact 

on synovial cell behavior. Additionally, the inflammatory response of synovial fibroblasts has been investigated, shedding 

light on the regulation of synovial cell growth by polypeptide growth factors. Chondrocytes have been extensively utilized 

in the study of cartilage regeneration and the development of high-quality cartilage. Studies have focused on the potential 

of aged human articular chondrocytes for cartilage regeneration, aiming to reverse chondrocyte aging and enhance 

cartilage regeneration. Furthermore, the role of microRNA-224 in suppressing osteoblast differentiation by inhibiting 

SMAD4 has been investigated, providing insights into the molecular mechanisms underlying osteoblast differentiation 

and function. 

Osteoblasts have been studied for their role in bone formation and bone-related diseases, with a focus on the regulation 

of osteoblast proliferation and differentiation. The knockout of the BK channel in osteoblasts has been investigated to 

determine its impact on osteoblast function and bone formation. The proliferative actions of parathyroid hormonerelated 

protein (PTHrP) in chondrocytes have been studied, highlighting the role of the cyclin-dependent kinase inhibitor 

p57Kip2 in mediating PTHrP-induced proliferation in chondrocytes. 

Circulatory system 

We provide a diverse array of cells from different animal species, isolated from various arteries and veins. Our offerings 

include endothelial cells, MSCs, fibroblasts, peripheral blood cells with a variety of markers, NK cells, monocytes, and 

blood cells. Additionally, we offer MSCs with GFP and RFP tags, as well as smooth muscle cells, with available cell RNA. 

Mesenchymal stem cells (MSCs) have been extensively studied and utilized in various research and clinical applications 

due to their regenerative and immunomodulatory properties. MSCs have been identified and isolated from different 

sources, including the umbilical cord, bone marrow, adipose tissue, and other tissues. These cells have shown potential 

for use in tissue engineering, regenerative medicine, and immunotherapy. The expression of specific surface markers and 

the characterization of MSCs from various sources have been the focus of numerous studies, aiming to understand their 

properties and potential applications. 

Peripheral blood cells with a variety of markers have been extensively studied for their role in the immune response and 

as a source of hematopoietic stem cells. These cells have been used in the study of immune cell populations, including T 

cells, B cells, and natural killer (NK) cells. The expression of specific markers on peripheral blood cells has been investigated 

to understand their functions and to develop targeted therapies for various diseases. NK cells, a type of cytotoxic 

lymphocyte, have been studied for their role in the immune response against infected or malignant cells. These cells have 

been investigated for their potential use in cancer immunotherapy and as a treatment for viral infections. The expression 

of specific markers on NK cells has been studied to understand their activation and cytotoxic functions. 

Monocytes, which are a type of white blood cell, have been extensively studied for their role in the immune response 

and as precursors to macrophages and dendritic cells. These cells have been investigated for their potential use in 

immunotherapy and as a source of antigen-presenting cells. The expression of specific markers on monocytes has been 

studied to understand their differentiation and immune functions. 

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Muscles 

We provide a diverse range of skeletal muscle cells and endothelial cells from various tissues and individuals, including 

diseased muscle cells and osteoblasts from different species. Additionally, we offer total RNA from skeletal muscle 

tissues, multinucleated myotubes, and RNA from stimulated or differentiated osteoblasts. 

Skeletal muscle cells have been extensively studied for their role in muscle regeneration, myogenesis, and the development 

of tissue engineering strategies for muscle repair. These cells have been used to investigate the molecular mechanisms 

underlying muscle development, regeneration, and the pathogenesis of muscle-related disorders. Total RNA from skeletal 

muscle tissues has been utilized in gene expression studies, transcriptome profiling, and the identification of regulatory 

pathways involved in muscle development, function, and disease. These studies have provided insights into the molecular 

signatures of skeletal muscle tissues and the dynamic changes in gene expression during myogenesis and muscle 

regeneration. 

Multinucleated myotubes, which are formed during myogenesis, have been studied for their role in muscle development, 

contractile function, and the regulation of muscle-specific gene expression. These structures have been used as an in 

vitro model to investigate muscle differentiation, fusion, and the molecular events underlying myotube formation. 

Differentiated osteoblasts have been extensively utilized in the study of bone formation, mineralization, and the 

regulation of skeletal development. These cells have been investigated for their potential role in bone tissue engineering, 

the study of osteogenesis, and the identification of molecular pathways involved in bone remodeling and repair. 


Custom Cell Isolation 

Researchers seeking tailored solutions can benefit from our custom cell isolation services. We offer the flexibility to use 

our standard protocols or your preferred methods, all at affordable rates. For precise cell isolation, we recommend our 

top-quality VitaCyte enzymes, particularly when working with sensitive cells or when accurate surface marker expression 

is essential, as in the case of hepatocytes. 

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Tissues 

We provide researchers with high quality human tissue samples sourced from various organs. These samples exhibit 

high RIN scores and are available from both healthy and diseased donors. Our extensive donor information includes 

details such as gender, age, ethnicity, BMI, cause of death, smoking and alcohol use history, HLA typing, and past 

medical records. 

Applications 

Spinal sections encompass a wide range of uses, including target profiling and engagement, spatial omics analysis, 

functional and structural interrogation, and their role in CNS drug discovery. Additionally, they are crucial in studying 

neurotoxicity and neuropathy, contributing significantly to the field of neurological research. 

Lung sections find application in studying cytokine release, gene expression, immune cell activation, transcriptomics, and 

proteomics. They are instrumental in investigating airway contraction and relaxation dynamics and the regulation of blood 

vessel constriction and dilation, making them vital tools for respiratory research. 

Heart sections play a pivotal role in target expression and localization studies, spatial omics analysis, functional assays, 

and action potential measurements. They are essential for understanding proarrhythmia, inotropic effects, force 

production, vascular tone regulation, and the assessment of chamber-specific efficacy and safety, thereby contributing to 

cardiovascular research and drug development. 

Dorsal root ganglia sections are employed in target profiling and engagement, as well as functional and structural 

interrogation. They play a crucial role in pain and itch drug discovery, as well as research related to neurotoxicity and 

neuropathy, enhancing our understanding of sensory and peripheral nervous systems. 

Brain sections offer valuable insights into target expression and localization, spatial omics, immunocytochemistry, and 

the application of RNAscope technology. They are indispensable in neuroscientific research, providing a foundation for 

understanding neural processes and cellular interactions in the brain. 

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Cell and Tissue RNA 

The use of RNA from various cells and diseased cells of individuals offers a wide range of applications in research and 

medicine, including: 

• Gene Expression Profiling: Analyzing RNA from different cells allows researchers to profile gene expression 

patterns, which can provide insights into the cellular functions and regulatory mechanisms. 

• Disease Biomarker Discovery: Differential gene expression analysis using RNA can help identify potential 

biomarkers associated with diseases, aiding in early diagnosis and monitoring of various conditions. 

• Drug Development and Testing: RNA from disease-specific cells can be used to screen potential drug 

candidates, evaluate their effectiveness, and assess their impact on specific genetic pathways. 

• Precision Medicine: RNA analysis can guide the development of personalized treatment strategies by 

understanding the genetic basis of individual diseases and tailoring therapies accordingly. 

• Understanding Disease Mechanisms: Investigating RNA from diseased cells can provide valuable information 

about the underlying molecular mechanisms of various conditions, which is crucial for advancing our 

understanding of diseases. 

• Functional Genomics: RNA interference (RNAi) and CRISPR-based technologies can be applied to manipulate 

gene expression in cells, enabling the study of gene function and regulation. 

• Comparative Genomics: Comparing RNA from different cell types and states can help researchers better 

understand the genetic variations and functional differences between healthy and diseased cells. 

• Stem Cell Research: RNA analysis is critical for characterizing the differentiation and pluripotency of stem cells, 

which is vital in regenerative medicine and tissue engineering. 

• Viral and Pathogen Detection: RNA-based techniques can be used to detect the presence of viral RNA, making 

it a valuable tool in diagnosing infectious diseases. 

• Biotechnology and Biomedical Research: RNA is essential for the development of molecular tools, such as RNA 

vaccines, RNA-based therapeutics, and RNA sequencing (RNA-Seq) technologies. 

However, the production of the same involves a time-consuming and multi-step process, starting from the dissociation 

of tissues and hoping for an adequate yield of cDNA. Instead of navigating through these time-intensive procedures that 

span weeks, just purchase our RNA isolated from different tissues and individuals with disease. We also offer RNA 

isolation kits for those who prefer to undertake the work independently. 

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Cell Culture Media 


Cell Culture Media can be classified on the basis of their function 

• Isolation 

• Growth/Expansion 

• Plating 

• Transfection 

• Maintenance 

• Differentiation 

• Starvation 

• Customized media 

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Cell culture media play a pivotal role in establishing an environment conducive to optimal cellular growth and function. 

Fetal Bovine Serum (FBS), a commonly used additive, provides essential nutrients, growth factors, and hormones crucial 

for cell proliferation. Ethical and regulatory considerations prompt researchers to explore animal origin-free alternatives, 

such as Human Platelet Lysate (hPL), derived from human platelets, ensuring a consistently ethically sourced supply. To 

exercise even greater control, defined media exclude undefined components, offering precision in shaping culture 

conditions. 

Key components, including the buffer system for pH stability, carbon sources for energy, pH indicators for acidity level 

monitoring, antibiotics for contamination prevention, and metabolites like amino acids, vitamins, and minerals for 

nutritional support, contribute to the intricate balance. While serum-rich in nutrients introduces variability, defined media 

empower researchers with explicit control over each component, facilitating fine-tuning of the culture environment. This 

depth of understanding enables scientists to tailor media formulations to meet specific cellular requirements, fostering 

successful and reproducible cell culture experiments. Ensuring the sterility and quality of individual components is 

imperative for achieving GMP (Good Manufacturing Practice) quality in the final media, highlighting the importance of 

meticulous handling and stringent quality control measures. 

Fetal Bovine Serum (FBS) 

FBS is derived from the blood of fetal bovines and serves as a rich source of nutrients, growth factors, and hormones 

essential for cell culture. The production of FBS involves collecting blood from fetal calves during the slaughter process. 

After collection, the blood is processed to extract the serum, which is then subjected to filtration and heat inactivation to 

eliminate potential contaminants. FBS is graded based on its origin, processing methods, and the absence of contaminants. 

Research-grade FBS is commonly used in basic research, while GMP (Good Manufacturing Practice) grade adheres to more 

stringent quality control standards, making it suitable for the production of biopharmaceuticals or clinical applications. 

Advantages: 

• Rich in nutrients and growth factors, supporting a wide range of cell types. 

• Well-established and widely used in cell culture applications. 

• Enhances cell attachment, proliferation, and viability. 

Disadvantages: 

• Batch-to-batch variability can impact experimental consistency. 

• Concerns about ethical and regulatory issues related to its production. 

• Potential risk of introducing contaminants like mycoplasma. 

Human Platelet Lysate (hPL) 

HPL is an animal origin-free alternative to FBS, often chosen to address ethical concerns and regulatory restrictions. Its 

production involves the collection of human platelets, typically obtained from donor blood. HPL grades vary based on 

specific quality parameters. Fibrinogen-depleted hPL eliminates fibrinogen, reducing the risk of clot formation in cultures. 

Gamma-irradiated hPL undergoes irradiation to ensure sterility. These different grades cater to diverse applications, with 

researchers selecting the grade that aligns with their specific needs, whether it be minimizing the risk of contamination, 

ensuring sterility, or meeting regulatory requirements. The flexibility in hPL grades allows for customization based on the 

desired characteristics for specific cell culture applications. We also offer AB serum, A serum, BSA, HAS and other 

supplemented media with ECGS and BPE. 

Advantages: 

• Animal origin-free, addressing ethical and regulatory concerns. 

• Consistent composition, reducing batch-to-batch variability. 

• Supports cell growth and expansion comparable to FBS. 


• Costlier compared to FBS. 

27


• May require additional optimization for specific cell types. 

• Availability may be limited compared to FBS. 

• 

Animal Origin-Free Media 

Animal origin-free (AOF) media, on the other hand, prioritizes the exclusion of any components sourced directly from 

animals, such as serum, addressing ethical concerns, regulatory constraints, and contamination risks. While AOF media 

eliminates the use of animal-derived products, it may still contain complex, chemically defined components. This broader 

category provides an alternative for researchers seeking to create a culture environment free of animal-origin components 

while allowing for flexibility in the specific formulation. 

Advantages: 

• Completely devoid of any animal-derived components, addressing ethical concerns and regulatory 

considerations. 

• Reduced risk of introducing contaminants from animal sources, ensuring a cleaner and more controlled culture 

environment. 

• Suitable for applications where the presence of animal-derived components is restricted, such as in the 

production of therapeutic proteins for human use. 


• Can be more expensive compared to media containing animal-derived components. 

• May require additional optimization for specific cell types, as the absence of certain factors present in animalderived 

products may impact cell growth or function. 

• Availability of well-defined formulations for all cell types may be limited. 

Defined Media 

Defined media represent a meticulous approach to cell culture formulation, offering researchers precise control over every 

component's composition. By excluding undefined or complex components, defined media eliminate batch variability, 

ensuring reproducibility in experimental outcomes. This level of specificity is particularly advantageous for detailed 

research where a clear understanding and manipulation of the nutritional and signaling factors influencing cell behavior 

are crucial. Defined media are ideal for experiments requiring consistency, allowing researchers to tailor the culture 

environment with exacting precision. 

Advantages: 

• Offers precise control over the composition of the media, allowing for reproducibility and consistency between 

batches. 

• Eliminates batch-to-batch variability associated with complex, undefined components, promoting reliability in 

experimental outcomes. 

• Ideal for research where a clear understanding and manipulation of individual components are crucial. 


• More expensive compared to media containing complex, undefined components. 

• Optimization may be required for specific cell types, as the absence of certain factors present in undefined 

media might affect cell behavior. 

• The formulation process can be complex, requiring careful consideration of the nutritional needs of the cells. 

28


Defined media from our portfolio: 

Cat# Product Quantity Application 

PB-YSP002 CellCor TM CD MSC 500 ml Human MSC Expansion, 

GMP 

PB-YSP017 CellCor TM EXO CD 500 ml Human MSC expansion & 

Exosome production 

PB-YSP018 

CellCor TM Keratinocytes 

CD AOF 

500 ml Culture of human 

keratinocytes 

PB-YSP019 CellCor TM MSC CD AOF 500 ml MSC Expansion, animal 

origin free; RUO & GMP 

PB-YSP007 CellCor TM DPC CD 500 ml Culture of human dermal 

papilla cells 

PB-C-MH-350-3111 

PB-C-MH-360-8699 

PB-C-MH-895-0099-d 

PB-C-MH-100-2199-d 

PB-C-MH-100-4099-d 

PB-C-MH-300-1099 

PB-C-MH-341-7499 

PB-C-MH-350-0099 

PB-C-MH-352-0096 

PB-C-MH-400-9099-d 

PB-C-MH-442-3699-M 

Cellovations Bronchial 

Epithelial Cell Growth 

Medium Kit, defined 

Cellovations Corneal 

Epithelial Cell Growth 

Medium Kit enhanced, 

defined 

Cellovations Co-Culture 

Growth Medium AE Kit 

Cellovations Endothelial 

Cell Growth Medium Kit 

enhanced, defined 

Cellovations 

Microvascular Endothelial 

Cell Growth Medium Kit 

enhanced, defined 

Keratinocyte Growth 


defined 

Oral Epithelial Cell 

Growth Medium Kit, 

defined 

Airway Epithelial Cell 


defined 

Renal Epithelial Cell 


defined 

Fibroblast Growth 

Medium Kit defined 

Adipocyte Maintenance 


defined 


bronchial epithelial cells 

500 ml Culture of human corneal 


500 ml Co-culture of Human 

adipocytes and 

endothelial cells 


endothelial cells from 

large vessels under FBSfree 

and defined 

conditions 


endothelial cells from 

small vessels under FBSfree 

and defined 

conditions 


keratinocytes 

500 ml Culture of human oral 


500 ml Culture of human airway 


500 ml Culture of human renal 



fibroblast 

500 ml Maintenance of human 

adipocytes 

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Isolation Media 

Isolation media is crucial for extracting specific cell types from tissues or complex cell mixtures. For instance, the use of 

collagenase-containing media aids in breaking down the extracellular matrix during pancreatic tissue processing, allowing 

the isolation of primary pancreatic islet cells. 

Growth/Expansion Media 

All-in-one growth/expansion media is designed for robust cell proliferation, providing an environment optimized with 

growth supplements. For example, DMEM supplemented with FBS and growth factors supports the expansion of human 

fibroblast cells, ensuring healthy and rapid cell division. 

For expansion of MSCs you can use CellCor TM MSC CD AOF (available in RUO & GMP quality) or CellCor CD MSC 

(available only in GMP quality) media and the CellCor DPC CD for Dermal papillary cells. Using this media for MSCs and 

DPCs is advantageous as it has similar cell proliferation as in serum media, can maintain cell identities such as morphology, 

genetic stability and gene expression and is consistent as compared to serum containing media. 

Plating Media 

Plating medium is essential for experiments involving specific cell types, such as neurons, requiring specific conditions for 

proper adherence. Neurobasal Medium with B27 Supplement, for instance, provides tailored nutrients to support the 

attachment and growth of primary neurons on culture surfaces. 

Transfection Media 

Transfection medium is tailored for efficient introduction of genetic material into cells. Using a combination of 

Lipofectamine 3000 Transfection Reagent and Opti-MEM Reduced Serum Medium facilitates the successful 

transfection of DNA or RNA into various cell lines for genetic studies. 

Maintenance 

Maintenance medium preserves cells in a specific state without inducing differentiation. It is used for maintaining 

mesenchymal stem cells in their undifferentiated state during prolonged culture, ensuring the stability of cell characteristics. 

Differentiation 

Differentiation medium guides cells into specialized forms and functions. We offer various differentiation media for MSCs 

and Adipocytes. Adipogenic Differentiation Medium induces the differentiation of preadipocytes into mature adipocytes, 

providing specific inducers for the adipogenic pathway. We also offer differentiation media for MSCs which can help in tridifferentiation 

of MSCs and causes less senescence than traditional serum containing media. 

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Starvation 

Starvation medium, devoid of serum nutrients or growth factors, is used to synchronize the cell cycle or study cellular 

responses under nutrient-deprived conditions. RPMI 1640 Medium without serum serves as an example, providing 

controlled conditions for experiments requiring a synchronized cell population. 

Customized media 

We offer customized media with or without various supplements as the use of media without phenol red, glutamine, and 

other supplements has been a subject of interest in various research areas. Phenol red, commonly used as a pH indicator in 

tissue culture media, has been found to have significant estrogenic activity, raising concerns about its potential impact on 

the study of estrogen-responsive cells in culture. Glutamine, an amino acid, has been studied for its anti-fatigue effects in 

sports nutrition, with observations on its impact on plasma glutamine levels and time to exhaustion. The role of common 

cell culture media supplements, including phenol red, penicillin/streptomycin, L-glutamine, and β-mercaptoethanol, has 

been investigated in the formation of gold nanoparticle protein corona, aggregation state, and cellular uptake, shedding 

light on their influence on cellular interactions. The impact of tissue culture components, including phenol red, on energy 

loss in cell culture equipment has been studied, validating the use of phenol-red containing media as physiologically relevant 

when examining light-culture system interactions. 

Glutamine supplementation has been the subject of clinical trials and systematic reviews to evaluate its effects on 

cardiometabolic risk factors and inflammatory markers, contributing to the understanding of its potential role in modulating 

plasma glutamine concentration and inflammation. Moreover, the formation of low-density plasma in aqueous biological 

media without phenol red supplementation has been achieved, demonstrating the influence of media components on 

plasma formation. Thereby for your special needs we can offer customized media if you can think it we can make it. 

& Cold management 

31

Cell Culture Tools 

• Cryo- and Cold-Management 

• Cell Culture supplements 

• Extracellular Matrices 

• Cell Culture Reagents 

• Transfection Tools 

• Reprogramming Tools 

• Safety Tools 


Cryo- & Cold-Management 

Cold- and cryo-preservation are critical steps while working with tissues and 

cells. Noteworthy to explain the importance of optimal, reproducible, and 

reliable cell work in clinical trials, cell therapies and regenerative medicine 

applications for humans and animals. It is obvious that basic research, drug 

development and compound testing will need the highest possible quality 

of cellular systems, too. 

Preservation is a critical, and often underappreciated, step in all research 

and clinical projects where tissues and cells will be used. It is best to optimize 

this process as early as possible to ensure reliable research, predictive 

compound testing, effective cellular therapies and highest possible 

cell/tissue quality. 

Cells can be isolated via cell separation (magnetic or non-magnetic procedures) or by tissue dissociation enzymes 

(collagenase & protease). In both cases the quality of cell source will be a critical factor. Cold preservation using special 

media which will help to keep the quality of tissues as high as possible. In many procedures cold- as well as cryo-preservation 

will be done. 

Cold-Preservation-Hypothermic Storage 

Storage of tissues and cells at low temperatures (4-10°C) for short periods (hours up to days) is defined as hypothermic 

storage. In the field of regenerative medicine cold preservation is an extension of principles and technologies used for 

whole-organ storage. 

Different media are available in the market for this application which were developed in many cases long before the 

explosion in cell therapy and tissue engineering. New media like HypoThermosol-FRS (BioLife) or Thermo-Gold (Revive) 

take the effects of cold shock damage (via stress pathways which are involved in necrosis and apoptosis) into account. 

Normally, cell or tissue function will be restored after 24 hours in culture at 37°C. 

Nevertheless, it is important to observe the post hypothermic storage viability for a week. Cells and tissues showing a high 

viability after warming can show a dramatic decrease after a few days. These effects depend on tissue or cell type. 

32


Cold-Preservation Products: 

HypoThermosol ® FRS 

Thermo-Gold TM 

HypoThermosol FRS (HTS-FRS) is an optimized 

hypothermic (2-8°C) preservation media that enables 

improved and extended preservation of cells, tissues and 

organs. HTS-FRS is uniquely formulated to address the 

molecular-biological response of cells during the 

hypothermic preservation process. HTS-FRS includes key 

ions at concentrations that balance the intracellular state 

at hypothermic temperatures. Additional components 

include pH buffers, energy substrates, free radical 

scavengers, and osmotic/oncotic stabilizers. 

Thermo-Gold is a unique, patented, optimized 

cold storage and shipping media. It is designed to 

provide high quality of tissue or cell viability and 

functionality during storage at 2-8°C. This 

proprietary, high quality produced medium 

reduced the tissue and cell shock of cold storage 

situation. Thermo-Gold includes components that 

scavenger for free radicals, provide pH buffering, 

oncotic/osmotic support, energy substrates and 

electrolytes balance for plasma membrane 

stabilization due to membrane potential 

maintenance at cold storage condition. 

• Ready-to-use 

• Serum-free, protein-free 

• Animal component-free 

• cGMP manufactured with USP grade components 

• FDA master file 

• Sterility, endotoxin and cell-based tested 

Cryo-Preservation 


• Serum-free, protein-free 

• Animal component-free 

• Fully defined 

• cGMP available 

• Sterility, endotoxin and cell-based tested 

Cryo-Preservation is a process were cells or tissues are preserved using temperatures between -80°C to -196°C applying 

cryoprotectants. Many protocols exist for primary cells, stem cells and cell lines. We at PELOBiotech offer a broad range of 

different cryo-preservation media for different application and with different qualities. Many primary cells (adherent cells) 

are cryopreserved using the recommended culture medium plus 10-20% FBS and 10% DMSO. Reproducibility can become 

an issue as different FBS lots are used which normally were not tested for this application or cell type. In addition, FBS 

contains exosomes which will might have unwanted effects on cells and tissues. 

Immune cells as well as stem cells are mostly cryo-preserved in commercial cryomedia which are produced under GMP 

conditions. These cells are more fragile and a spontaneous differentiation of stem cells should be avoided. 

For ES and iPS cells DMSO-free cryo-media become more of interest to avoid the possible negative effects of DMSO. 

DMSO in general is a toxic agent and can prevent the "normal" differentiation of stem cells. This is one reason why many 

groups try to develop DMSO-free cryo-media. Beside DMSO-containing cryo media we offer DMSO-free media as well as 

protein-free cryo media for different applications. 

Figure 1: Process flow chart cryo-preservation 

33


Media for Cryo-Preservation 

The classical way to cryopreserve primary cells 

is the usage of the culture medium plus 10- 

20% FBS and 10% DMSO. After trypsination 

of adherent growing cells, neutralization of 

the used trypsin and centrifugation cells are 

resuspended in the freezing medium (e.g. 

Endothelial Cell Growth Medium + 10% FBS 

+ 10% DMSO). 

As alternative commercially available freezing 

media can be used (Cryo-ROS, Cell freezing 

medium and others) which contain 10% 

DMSO. These media are available and tested 

for different cell types e.g. blood cells, 

hematopoietic stem cells, melanocytes, 

macrophages, microglia and neural cells. 

In recent years companies approached into the 

market who are specialized in cryo-preservation and tissue storage media. One of our partners, BioLife Solutions, Seattle, has 

developed a series of DMSO containing cryo media which are produced under GMP conditions and are suitable and tested 

for a high number of cells. CryoStor CS 2, 5 and 10 is pre-formulated with USP grade DMSO of 2, 5 or 10%. The media is 

based on the novel HypoThermosol formula. 

Cryo-Preservation Media 

CryoStor® 

CryoStor® CS10 is a state-of-the-art cryopreservation medium designed to maximize postthaw 

cell recovery and viability in extremely low temperatures ranging from -70°C to - 

196°C. This ready-to-use solution is serum-free, animal component-free, and cGMPmanufactured, 

ensuring a safe and protective environment for the cryopreservation of a 

diverse array of sensitive cell and sample types. The formulation, containing 10% dimethyl 

sulfoxide (DMSO) along with other essential ingredients, is meticulously crafted with USP-grade components to minimize 

variability. This makes CryoStor® CS10 an ideal choice for e.g. preserving myeloma cell lines, human pluripotent stem 

cells, blood-derived cells. 

Available in various convenient formats, CryoStor® CS10 caters to the cryopreservation needs of B Cells, CHO Cells, 

Hematopoietic Stem and Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem and Progenitor 

Cells, Monocytes, Myeloma, NK Cells, Pluripotent Stem Cells, T Cells across different species, including Human, Mouse, 

Non-Human Primate, and Rat. Whether applied in Cord Blood Banking, Epithelial Cell Biology, Immunology, or Stem Cell 

Biology, CryoStor® CS10 stands out as an animal component-free, serum-free solution, ensuring the integrity and viability 

of preserved cells. We also offer the same with 2% and 5% DMSO. 

Cryo-Preservation by Vitrification 

Vitrification is the solidification of a liquid without crystallization and growth of ice. It is an ultrafast 

freezing method which is used for ES and iPS cells successfully. StemCell Keep is a DMSO-, 

serum-, protein- and xeno-free cryopreservation medium for ES/iPS cells by vitrification. 

Right: Vitrification is successful 

Left: Recrystallization happened vitrification failed) 

Procedure 

1. Just add StemCell Keep to your cells after centrifugation and transfer the vial within 1 minute to liquid nitrogen. 

2. Keep the vial for 1-2 minutes in liquid nitrogen and check the process. 

3. Store cells in your liquid nitrogen tank. 

34


Thawing: 

1. Just take the vial out of liquid nitrogen and add 1 ml warmed medium. 

2. Mix carefully and add cells to a vial with additional 9 ml medium. 

3. Seed cells to your feeder plate or feeder-free plate system. 

Cryo-Preservation Products 

Cat# Product Recommended or used for Quantity 

FBS containing Cryo Media 

PB-10002-01 Cryo-ROS Cell Lines & Primary Cells 100 ml 

PB-10002-01-20 Cryo-ROS Cell Lines & Primary Cells 20 ml 

Serum-free, Protein-free, DMSO-containing Media 

PB-202102 CryoStor® CS 2 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bottle 


PB-205202 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 100 mL bag 

PB-205373 CryoStor® CS 5 Adult Stem Cells, Primary Cells, Cell Lines 10 mL vial 


PB-210202 CryoStor® CS 10, Bag Adult Stem Cells, Primary Cells, Cell Lines 100 ml bag 

PB-210210 CryoStor® CS 10, Bag Adult Stem Cells, Primary Cells, Cell Lines 1 L bag 

PB-210373 CryoStor® CS 10 Adult Stem Cells, Primary Cells, Cell Lines 10 mL vial 

PB-210374 CryoStor®CS 10 Adult Stem Cells, Primary Cells, Cell Lines 16 mL vial 

PB-210473 CryoStor® CS 10, Syringe Adult Stem Cells, Primary Cells, Cell Lines 10 mL syringe 

PB-10003-01 Cryo-Gold 

CHO Cells, Hybridoma Cells, Adult Stem 

Cells, ES/iPS Cells, Primary Cells, Sperm, 

Oocyte, Embryo, Cell Lines 

100 ml 

PB-10003-01-20 

Cryo-Gold 

CHO Cells, Hybridoma Cells, Adult Stem 

Cells, ES/iPS Cells, Primary Cells, Sperm, 

Oocyte, Embryo, Cell Lines 

20 ml 

PB-10004-01 

Cryo-Jin 

iPS Cells, Human Hepatocytes, Neural 

Cells, Islet Cells, Oocyte 

100 ml 

PB-10004-01-20 

DMSO-free media 

CPL-A1 

Cryo-Jin 

iPS Cells, Human Hepatocytes, Neural 

Cells, Islet Cells, Oocyte 

35 

20 ml 

NutriFreez D10 Human embryonic stem cells, iPSC, MSCs 50 mL 

CryoScarless 

Primate ES/iPS Cells, Mesenchymal Stem 

Cells, Cell Lines 

100 ml 

RCHEFM002 ReproCRYO ES/iPS Cells 50 ml 

Vitrification medium 

VPL-A1 StemCell Keep ES/iPS Cells 20 ml


Further products of interest: 

BloodStor® 

The storage of stem cells from cord blood, peripheral blood, and bone marrow is crucial due to their potential therapeutic 

uses in treating various diseases. Cord blood, in particular, contains hematopoietic stem cells (HSC) that can be used to treat 

conditions such as leukemia, lymphoma, sickle cell disease, and other blood, bone, immune, and metabolic disorders. 

Cord blood stem cells are being researched for their potential in regenerative medicine, where they may be used to induce 

healing and regenerate cells to restore tissues. Furthermore, peripheral blood and bone marrow are also valuable sources 

of stem cells for transplantation, with peripheral blood offering advantages such as easier and more common collection 

compared to bone marrow. Exploratory analyses have indicated that peripheral-blood stem cells may reduce the risk of 

graft failure, while bone marrow may reduce the risk of chronic graft-versus-host disease. 

Proper storage of these stem cells is essential to maintain their viability and functionality for future therapeutic use. Umbilical 

cord blood stem cells can be stored in cord blood banks for potential use in stem cell transplants, providing an "off-theshelf" 

source of stem cells unlike bone marrow. The ability to store umbilical cord stem cells for future use presents a 

significant clinical advantage. Moreover, the use of stem cells aspirated from bone marrow eliminates the need for the 

creation of a second surgical site for harvesting autogenous bone, highlighting the importance of proper storage and 

preservation of these cells. 

ThawStar® 

ThawStar® offers reproduceable thawing of cells this is important as studies have 

shown that cryopreserved cells, such as mesenchymal stromal cells (MSCs), display 

impaired immunomodulatory properties and reduced therapeutic potential 

immediately after thawing. This impairment can lead to faster complement-mediated 

elimination after exposure to blood, indicating a compromised functionality of the 

cells. The cryopreservation process has been found to affect the viability and 

metabolic function of cells, making them unsuitable for clinical use upon thawing. The alteration of the actin 

cytoskeleton due to cryopreservation can also impact cell function and survival post-thawing. 

The susceptibility of cryopreserved MSCs to T-cell mediated apoptosis and their altered biodistribution in vivo after 

thawing further emphasize the detrimental effects of improper thawing on cell functionality. Moreover, the impact of 

cryopreservation on cell migration ability and the alteration of the functional competency and survival of cells due to 

freezing and thawing further highlight the importance of proper thawing procedures to maintain cell functionality. It 

is evident that the processing, freezing, and thawing cycle can be detrimental to the cells, emphasizing the need for 

optimized thawing protocols to preserve cell potency and therapeutic effect. Furthermore, the use of cryopreserved 

products immediately after thawing has been associated with a higher failure rate compared to the use of fresh cells, 

underscoring the critical nature of proper thawing procedures. 

Cell Culture Supplements 

Human and Bovine Serum Products: 

• HSA Solution 25% (cGMP): Human Serum Albumin (HSA) is a versatile product used in various cell culture 

applications. It serves as a stabilizer for enzymes, a blocking agent in Western blots, and a component of cell 

culture media. Its high purity ensures consistent performance in diverse assays. 

• Human AB Serum, Converted from Octaplas®: This converted human AB serum is crucial for culturing primary 

cells, including hematopoietic cells and stem cells. It's xeno-free and virus-inactivated, making it suitable for a 

wide range of cell culture applications. 

• Human Serum Albumin (HSA) 25% CSS (cGMP): Closed System Solutions (CSS) of HSA is designed for 

biomanufacturing applications, such as the production of vaccines, therapeutic proteins, and cell therapies. 

• Bovine Serum Albumin (Fraction V): Bovine Serum Albumin (BSA) is a common protein supplement in cell 

culture media. It stabilizes enzymes and acts as a carrier protein for hormones, fatty acids, and other 

compounds. BSA is often used in immunocytochemistry, ELISA, and Western blotting. 

36


• Fetal Bovine Serum (FBS): FBS is a classic cell culture supplement known for its growth-promoting properties. It 

is used for the cultivation of a wide variety of cell types, including primary cells and established cell lines. FBS is a 

rich source of growth factors and nutrients. 

• Fetal Bovine Serum, Heat-Inactivated: Heat-inactivated FBS is treated to inactivate complement, making it 

suitable for specific cell culture applications where complement activity needs to be minimized. 

Growth Factors and Supplements 

• Endothelial Cell Growth Supplement (ECGS): ECGS is essential for the growth of endothelial cells and is often 

used in angiogenesis and vasculogenesis studies. 

• L-Glutamine-MAXIMUM Solution (100x): L-Glutamine is an amino acid vital for cell growth, and the 

MAXIMUM Solution ensures its stability in cell culture. 

• ITS Solution 100x: Containing insulin, transferrin, and selenium, ITS Solution enhances cell growth and is 

commonly used in serum-free media and bioprocessing applications. 

• UltraGRO Advanced Cell Culture Supplement: This advanced supplement, derived from human platelet 

lysate, promotes cell growth, expansion, and differentiation. It is suited for research and clinical applications, 

including regenerative medicine and tissue engineering. 

Exosome-Depleted Supplements 

Exosome-Depleted UltraGRO-PURE GI Research grade: A specialized supplement that excludes exosomes for research 

applications where exosome presence might affect the results. 

Xeno-Free and Defined Supplements 

• UltraGRO-PURE Cell Culture Supplement: A xeno-free supplement derived from human platelet lysate for 

applications where completely animal-free conditions are required. 

• Artificial Serum (Xeno-free): A xeno-free alternative to traditional serum, suitable for various cell culture 

experiments. 

• Artificial Serum (Animal-free): This animal-free supplement is ideal for researchers aiming to eliminate 

animal-derived components from their culture media. 

• Serum-Free RocketFuel: A serum-free medium supplement that provides essential nutrients for cell growth. 

• Serum-Free Recombinant RocketFuel: A serum-free and recombinant supplement designed for improved 

growth conditions. 

• BIT Admixture 100: A serum substitute that supports cell growth while eliminating the need for traditional 

serum. 

• Serum Replacement Supplement (Animal-free & Defined): An animal-free, defined supplement for 

researchers working with highly controlled conditions, including stem cell culture and regenerative medicine. 

Specific Tissue Extracts 

• Bovine Brain Extract (BBE): Bovine brain extract is used for specific cell culture applications, particularly in the 

neuroscience field. 

• Bovine Brain Extract-Hammond (BBE-Hammond): Hammond's formula BBE is a specialized supplement used for 

culturing neuronal cells. 

• Bovine Pituitary Extract (BPE)-Hammond: BPE is used in specific cell culture applications, especially for the growth of 

certain cell types. 

Miscellaneous Supplements 

• CultureBoost: A general-purpose cell culture supplement. 

• CultureBoost-R: Another cell culture supplement for promoting cell growth and health. 

• NEAA Cell Culture Supplement: Non-essential amino acids supplement that supports cell culture. 

37


• B28 Neuron Culture Supplement: Specifically formulated for neuronal cell culture, aiding in the growth and 

differentiation of neurons. 

• Neural Cell Supplement: Designed for neural cell culture, particularly primary neuronal and glial cell cultures. 

• Fetal Bovine Serum EU approved (Cell culture tested): FBS that complies with European Union regulations, ensuring 

quality and consistency. 

• ECGF Bovine (Crude Extract, cell culture grade): A crude extract of bovine endothelial cell growth factor used for 

promoting angiogenesis and vasculogenesis in cell culture. 

These products are crucial for maintaining, expanding, and experimenting with various cell types, and they find applications 

in diverse fields such as regenerative medicine, stem cell research, tissue engineering, drug development, and fundamental 

cell biology studies. 

Extracellular Matrices 

Cell Culture experiments are very often done under relative strange 

conditions as important factors like stiffness and cell environment 

is not reflected in the chosen experimental designs. In the last 10 

years cell culture improved a lot as the cellular microenvironment, 

surface stiffness and physiologic culture conditions are more taken 

into consideration. 

Coating of cell culture surfaces is done in most experiments to 

increase cell attachment and cell spreading. Used are un-defined 

mixtures of extracellular proteins derived from various sources. 

We offer ready-to-use ECM solutions from different sources, 

gelatin and purified extracellular matrix proteins. 

The MicroMatrix Array gives you the chance to evaluate the best matrix 

composition for your cellular systems on a single slide. 36 different ECM mixtures 

are used which are plotted 9 times per square. The assay was used so far for cell 

adherence and stem cell differentiation experiments. 

MicroMatrix 36 | 

MicroMatrix 36-4Pack | 

Cat#: PB-MM012011 – 1 slide 

Cat#: PB-MM012011-4 – 4 slides 

Pic: Micromatrix at work 

Gelatin & Coating mixtures 

We offer various ECM mixtures to coat the culture vessels to enhance cell attachment, cell spreading and cell growth. 

• Gelatin 

• Poly-D-Lysine & Poly-L-Lysine 

• Gelatin & Poly-L-Lysine 

• EHS-Matrix derived from mouse Engelbreth-Holm-Swalm tumor cells; the EHS matrix is widely used for stem cells, 

organoid and spheroid cultures 

• ExtraGel an optimized and effective growth scaffold for organoid, pluripotent stem cell, and advanced 3D 

culture applications. 

• Neuron Plating Solution 

• Speed Coating Solution 

• Stem Cell Matrix XF for pluripotent stem cells 

38


Organ derived NativeCoat TM – 2D ECM Surface Coatings 

Coat any cell culture surface with NativeCoat ECM to give your cells the full milieu of proteins and growth factors 

present in the native tissue of interest. Available are the following products derived from healthy porcine acellular organs: 

• NativeCoat Blood Vessel ECM 

• NativeCoat Bone ECM 

• NativeCoat Cartilage ECM 

• NativeCoat Colon ECM 

• NativeCoat Heart ECM 

• NativeCoat Intestine ECM 

• NativeCoat Kidney ECM 

• NativeCoat Liver ECM 

• NativeCoat Lung ECM 

• NativeCoat Pancreas ECM 

• NativeCoat Skin ECM 

• NativeCoat Stomach ECM 

Just call us, if you need further information! 

Biomatrix Proteins 

Beside ECM mixtures we also offer purified biomatrix proteins derived from various sources. 

• Collagen Type I, II, III, IV 

• Fibronectin (human plasma derived; also available in GMP quality and virus inactivated) 

• iMatrix 221 (Recombinant Laminin for Cardiomyocytes & Myoblast) 

• iMatrix 411 (Recombinant Laminin for Endothelial Cells) 

• iMatrix 511 (Recombinant Laminin for ES/iPS Cells) 

• Vitronectin 

ExtraGel – the equivalent of Matrigel ® 

Scilia Extragel represents a reconstituted matrix hydrogel derived from basement membrane components sourced from 

mouse tumor tissues, notably abundant in extracellular matrix proteins. This matrix hydrogel primarily consists of laminin, 

collagen IV, and heparan sulfate proteoglycans. It also incorporates essential growth factors such as epidermal growth 

factor, platelet-derived growth factors, nerve growth factors, basic fibroblast growth factor, transforming growth factorbeta, 

and insulin-like growth factors. 

• Rigorously tested for LDEV, fungi, bacteria, and mycoplasma, Scilia Extragel offers a convenient laboratory 

solution devoid of pollutants. 

• Our commitment to quality is evident through strict inspections to ensure product stability, consistency, and 

reliable performance across batches. 

• Compatible with a variety of cell culture media, this hydrogel allows for versatile applications. 

• With a single-batch manufacturing process capable of yielding up to 1L volume, Scilia Extragel guarantees a 

steady and substantial supply for your research needs. 

Hydrogels 

Hydrogels can be used for 2D & 3D cell culture as shown in the pictures below: 

Pic: Cells growing …on top of hydrogel| 

39 

… in a hydrogel (3D Cell Culture)


As the stiffness of plastic culture vessels is far higher even than of bones we can imagine how artificial these cultures 

might be. A thin layer of a hydrogel can be helpful, but the solution will be a tuneable hydrogel. 

Cell Culture Reagents 

Insulin and Insulin-Related Products 

• rHu Insulin (cGMP): Recombinant human insulin used in cell culture, especially for studies involving glucose 

metabolism, diabetes, and insulin signaling pathways. 

• Bovine Insulin: Insulin derived from bovine sources, often used for similar applications as human insulin in cell 

culture. 

• Porcine Insulin: Insulin derived from porcine sources, employed in cell culture experiments related to diabetes, 

metabolism, and insulin signaling. 

• Insulin Solution, Human, Recombinant: A recombinant human insulin solution suitable for various cell culture 

and research applications. 

Cell Culture Solutions and Buffers: What about passaging? 

• Hanks Balanced Salt Solution without Ca2+ and Mg2+: A balanced salt solution used for cell culture 

applications, including the maintenance and washing of cells. 

• HBSS (100 ml): Hank's Balanced Salt Solution in a 100 ml format, ideal for smaller-scale cell culture experiments. 

• Dulbecco Phosphate-Buffered Saline, 1X (DPBS): A common buffer for cell culture applications, used for cell 

maintenance and experimental procedures. 

• PBS Buffer (1X): Phosphate-buffered saline (PBS) at a 1X concentration, widely used for cell washing and 

dilution in various biological assays. 

Chemicals and Additives 

• Heparin, Sodium Salt: Heparin is used as an anticoagulant in cell culture, especially when working with primary 

cells, stem cells, and coagulation studies. 

• Phenol Red (1ml) (minimum 10 vial order): Phenol red is a pH indicator often added to cell culture media to 

visually monitor pH changes. 

• Phenol Red (10ml): A larger quantity of phenol red for extended cell culture experiments. 

• Antibiotic Antimycotic Solution (100x): A high-concentration solution of antibiotics and antimycotics for 

preventing bacterial and fungal contamination in cell cultures. 

• Sodium Hydroxide (100 ml): Sodium hydroxide is used for adjusting pH levels in cell culture media. 

• L-Glutamine: An amino acid commonly added to cell culture media to promote cell growth. 

• 0.155 M NaCl: A saline solution used for various biological and cell culture applications. 

• Calcium Chloride Solution: Calcium chloride is employed in cell culture for inducing calcium-dependent 

processes in cells. 

• Lysis Buffer (5 ml): A buffer used for cell lysis and the release of cellular contents in various assays. 

40


• Depolymerization Solution (50 ml): Used to depolymerize specific cellular components and study their 

properties. 

Reagents and Supplements 

• Penicillin/Streptomycin Solution (1:100): A mixture of penicillin and streptomycin, commonly used to prevent 

bacterial contamination in cell culture. 

• Bac-Off ® Antibiotic: A specific antibiotic solution used to prevent bacterial contamination in cell cultures. 

• Gentamycin-Amphotericin: An antimicrobial agent that includes gentamycin and amphotericin B, employed to 

prevent bacterial and fungal contamination. 

• Plating and Thawing Kit for Hepatocytes: A specialized kit designed for plating and thawing hepatocytes, 

commonly used in liver cell culture experiments. 

• Thawing Kit for Hepatocytes (suspension culture): A kit tailored for the thawing and culturing of hepatocytes in 

suspension. 

Transfection tools 

Cytofect Transfection Kits 

• Cytofect Epithelial Cell Transfection Kit: Designed for efficient transfection of epithelial cells, facilitating the 

introduction of foreign genetic material into these cells. 

• Cytofect Neuron Transfection Kit: Specialized for transfection of neurons, allowing researchers to manipulate gene 

expression in neuronal cells. 

• Cytofect Smooth Muscle Transfection Kit: Tailored for transfection of smooth muscle cells, making it possible to 

study gene functions in these cell types. 

• Cytofect Hepatocyte Transfection Kit: Optimized for transfection of hepatocytes, which is valuable for liverrelated 

research and gene manipulation in liver cells. 

• Cytofect Preadipocyte Transfection Kit: Targeted at preadipocytes, enabling efficient gene transfer into these 

cells, essential for studying adipocyte biology. 

• Cytofect Endothelial Cell Transfection Kit: Developed for endothelial cells, facilitating the introduction of genes 

into these cells, which is crucial for vascular biology research. 

• Cytofect Fibroblast Transfection Kit: Geared towards fibroblasts, allowing gene transfer to these cells commonly 

used in tissue engineering and wound healing studies. 

• Cytofect Cell Line Transfection Kit: A versatile kit suitable for transfecting various cell lines, making it a valuable 

tool for a broad range of research projects. 

• Cytofect HUVEC Transfection Kit: Targeted at human umbilical vein endothelial cells (HUVECs), supporting 

transfection in these specific cells. 

Cytofect Transfection Reagents and Enhancers 

• Cytofect-1 (CF1) and Cytofect-2 (CF2): Transfection reagents for use with various cell types, designed to enhance 

the efficiency of gene delivery. 

• Peptide Enhancer (PE): An enhancer that can improve the efficiency of transfection by assisting in the cellular 

uptake of genetic material. 

• Viral Enhancer (VE): Enhancer designed to improve viral transfection methods for gene delivery. 

Cytofect Sample Kits 

• Cytofect Endothelial Cell Transfection Sample Kit: A sample kit for transfecting endothelial cells to evaluate 

transfection efficiency and optimize transfection conditions. 

• Cytofect Epithelial Cell Transfection Sample Kit: Similar to the above but for epithelial cells. 

• Cytofect Fibroblast Transfection Sample Kit: Designed to facilitate testing of transfection conditions in 

fibroblasts. 

41


• Cytofect Cell Line Transfection Sample Kit: Enables to assess transfection conditions in various cell lines. 

• Cytofect Skeletal Muscle Cell Transfection Sample Kit: A sample kit for the evaluation of transfection conditions 

in skeletal muscle cells. 

• Cytofect HUVEC Transfection Sample Kit: Suitable for testing and optimizing transfection conditions in 

HUVECs. 

• Cytofect Smooth Muscle Cell Transfection Sample Kit: Provides a sample kit for smooth muscle cell transfection 

optimization. 

• Cytofect Chondrocyte Transfection Sample Kit: Designed for the evaluation of transfection conditions in 

chondrocytes. 

• Cytofect Mesenchymal Stem Cell Transfection Sample Kit: Used for optimizing transfection in mesenchymal 

stem cells. 

• Cytofect Hepatocyte Transfection Sample Kit: Supports transfection condition evaluation in hepatocytes. 

• Cytofect Preadipocyte Transfection Sample Kit: Offers a sample kit for preadipocyte transfection optimization. 

• Cytofect Neuron Transfection Sample Kit: Facilitates the evaluation and optimization of transfection conditions 

in neurons. 

Other Transfection Reagents 

• AteloGene Systemic Use and AteloGene Local Use Quick Gelation: Transfection reagents with specific applications 

for systemic or local gene delivery. 

• ProteoCarry (Protein Transfection Reagent): Designed for transfection of proteins into cells, allowing for the 

delivery of functional proteins to manipulate cellular processes. 

Reprogramming Tools 

RNA Transfection Kits and Components 

• Stemfect RNA Transfection Kit: A kit designed for the efficient transfection of RNA into cells, particularly useful 

for researchers working with RNA-based experiments. 

• StemRNA 3rd Gen Reprogramming Kit: A reprogramming kit tailored for the generation of induced pluripotent 

stem cells (iPSCs) from somatic cells. It includes key reprogramming factors to facilitate the conversion of 

differentiated cells into pluripotent stem cells. 

• Oct4 mRNA, Human (20 ug): This mRNA encodes the Oct4 transcription factor, a crucial component for 

pluripotency induction and reprogramming of somatic cells. 

• Sox2 mRNA, Human (20 ug): Encodes the Sox2 transcription factor, another essential component for cellular 

reprogramming and pluripotency maintenance. 

• c-Myc mRNA, Human (20 ug): This mRNA encodes the c-Myc transcription factor, which, when used in 

combination with other factors, can enhance the reprogramming process. 

Stem Cell and Pluripotency-Related Products 

• StemAb Alkaline Phosphatase Staining Kit II: A kit that helps visualize pluripotent stem cells, as alkaline 

phosphatase is often used as a pluripotency marker. 

• hES Cell Cloning & Recovery Supplement (5 x 100 µL): This supplement is designed for the maintenance and 

recovery of human embryonic stem (hES) cells, essential for their growth and propagation. 

42


Safety Tools 

Research from the FDA, ATCC, and others show that 5 % - 30 % of all cell cultures today are contaminated with 

mycoplasma. Mycoplasma contamination in cell culture can have profound effects on cellular behavior, leading to skewed 

experimental outcomes and jeopardizing the reliability of research findings. Mycoplasma are known to alter various 

cellular functions, including metabolism, proliferation, and gene expression. They can induce changes in cell morphology, 

disrupt cell cycle progression, and even trigger apoptosis. These alterations can confound experimental results, making it 

challenging to draw accurate conclusions about the impact of specific treatments or conditions on cells. Mycoplasma 

contamination can compromise the reproducibility of experiments, hindering the ability to validate and build upon 

scientific findings. By implementing stringent safety tools and practices, researchers aim to create a microenvironment 

that minimizes the risk of mycoplasma contamination, thus ensuring the fidelity of cell culture experiments and the 

reliability of scientific research. Adhering to strict sterile techniques, such as using sterile pipettes, gloves, and culture 

hoods, further reduces the likelihood of introducing contaminants. These safety measures are imperative not only for 

maintaining the scientific validity of cell culture experiments but also for safeguarding the well-being of researchers and 

preventing the spread of contaminants within the laboratory environment. 

Save time and ensure the integrity of your research with our meticulously curated cell lines—mycoplasma, virus, and 

contamination-free. Elevate your experiments with the confidence that comes from starting with pristine cells, 

allowing you to focus on groundbreaking discoveries rather than constant testing. Your research deserves the purity it 

needs, and our cell lines deliver reliability straight out of the vial. 

Mycoplasma Detection and Elimination Kits 

• BioMycoX Mycoplasma Elimination Kit: Designed to eliminate mycoplasma contamination from cell cultures, 

ensuring the integrity of experiments and cell lines. 

• BioMycoX Mycoplasma PCR Detection Kit: Provides a PCR-based method for the detection of mycoplasma 

contamination in cell cultures. 

• BioMycoX Mycoplasma qPCR Detection Kit: Utilizes quantitative PCR (qPCR) to detect and quantify 

mycoplasma contamination. 

• HiSense Mycoplasma PCR Detection Kit: Specifically designed for the PCR-based detection of mycoplasma 

contamination. 

• MycoQsearch Mycoplasma qPCR Detection kit EP: Offers a highly sensitive qPCR detection kit for mycoplasma, 

complying with European Pharmacopoeia (EP) standards. 

• MycoQsearchTM Plus Mycoplasma qPCR Detection kit EP (NEW_Dual Probes included for crosscontamination 

checking): This advanced qPCR kit includes dual probes to check for cross-contamination and 

meet EP standards. 

• SafeDry Mycoplasma PCR Detection Kit (8 strip x 6 ea): A mycoplasma PCR detection kit provided in an 8- 

strip format for convenient use. 

Mycoplasma Prevention and Contamination Products 

• BioMycoX Mycoplasma Prevention Spray (1L): Designed to prevent mycoplasma contamination in the 

laboratory environment, providing an additional layer of protection. 

43


3D Cell Culture 

Welcome to your 3D One-Stop-Cell Culture Shop, 

Competence in cell culture is PELOBiotech's standout feature. We aim to provide you with the best and most reliable results, 

allowing you to work with confidence and ease. At PELOBiotech, we take pride in our successful journey with 3D technologies. 

With one of our founders having contributed to the development and establishment of various systems now leading in the 3D 

industry, we are well-equipped to assist you in finding optimal solutions for your research. 

That's why we maintain a wide array of 3D technologies, providing ready-to-use 3D cell culture models and cell culture assay 

kits. We are continually exploring innovative 3D technologies for spheroid and organoid formation. 

We offer different 3D Technologies and 3D Models 

• Scaffold-free 

• Scaffold-based 

• Hydrogel-based 

3D CoSeedis TM 

EZ-Seed 

ABLE ® Biott ® Bioreactors 

EZSPHERE TM Multi-Well Plates and Dishes (AGC ® ) 

Organotypic Scaffolds 

SEEDEZ - glass microfiber scaffolds 

Alvetex® Scaffolds 

SpheroSeev 

• 3D Models Ready-to-Use 

3D Skin Model 

3D Airway Model 

• Dynamic Models 

Organotypic Hydrogels isolated from different organs 

PLMA photopolymerizable hydrogel, platelet lysate derived 

MatriMix 

Collagen, Fibronectin 

Extragel an equivalent to Matrigel ® 

HydroSeev to improve your hydrogel culture 

Phenodrive 

Midi & Microfluidic Systems 

PerfusionPAL - Scaffold based Perfusion Systems 

Contact us today and let us show you how we can help you meet your technical 

and budget targets and bring your research from bench to bedside. 

44


3D Scaffold-free 

3D CoSeedis 

The leading Swiss expert in Biostandardisation abc biopply ag has developed the innovative modular multi-purpose 3D 

Cell Co-Culture System 3D CoSeedis TM . 

Biostandardization respects clinical requirements already in the stage of molecular 

research and therefore can safe years in therapeutic development. With this unique and easy readout system a new standard 

in 3D Co-Culture is set. The novel scaffold-free 3D cell co-culture system consists of a unique conical agarose matrix array 

(CAMA) that allows the formation of spheroidal and non-spheroidal cell aggregates in a highly-reproducible and consistent 

manner. The conically shaped microwells within the array allow precise determination of aggregate volume and cell growth. 

Furthermore, the modular composition of the 3D system allows distance co-cultures and, consequently, the standardization 

of protocols. 

Key Features 

Matrix and Modular Set-up 

• Contact or distance co-cultures (fully separable) 

• Serum, serum-reduced or serum-free conditions possible 

Permeable Matrix 

• Allows long term 3D culture of cell aggregates 

• Efficient use of physically separated feeder cells (distance co-culture) 

• Cultivation in standard or defined media 

Multi-purpose Applicability 

• Applicable to various cell types (see Applicable Cell Systems) 

• Supports the formation of aggregates of spheroidal and non-spheroidal cell types 

• Highly reproducible cell aggregates 

• Suitable for high-throughput applications 

Unique and easy Readout 

• Integrated method for histological analysis (H&E, IHC, ISH, etc.) 

3D CoSeedis is validated for the following applications: 

• 3D growth monitoring by volumetric analysis 

• 

Life/dead TM 

• Viability assays 

• HTS/HCS 

• Automated microscopy 

• Immunohistochemistry and -fluorescence 

• RNA, DNA and protein profiling 

• Extracellular vesicle production/profiling (e.g. EV-free mesenchymal stromal/stem cell co-cultures) 

• Whole-mount staining 

• Colony forming assay for tumorsphere formation and clonogenic survival 

Mass production of homogenous and uniform organoids/spheroids by using 3D CoSeedis TM 

One of the most important topics of 3D cell culture is a smooth and robust transition of 2D cell culture to 3D. This will help 

to create standardized workflows and protocols which are the fundament of reproducible and consistent results. abc biopply 

45


designed 3D CoSeedis TM as a robust platform for mass-production of highly homogeneous and uniform organoids and 

spheroids which makes HTS/HCS applications. 

Available products sold together with single use or long-term licenses 

• 3D CoSeedis TM Chip200 (6 well format, 200 organoids per chip), Cat# C200 or C200-6 (6 chips) 

• 3D CoSeedis TM Chip880 (6 well format, 880 organoids per Chip), Cat# C880 

• 3D CoSeedis TM Chip680 (24 well format, 680 organoids per chip), Cat# C680 or C680-6 or C680-12 (12 chips) 

• Accessories 3D CoSeedis TM Spatula , Spatula for matrix handling, sterile, 

• Spheroid Formation Media 

• Single Use License: Get easy and fast access to our assays for in-house tests 

• Long-Term License: Get easily access to the assays of your choice and have your planning certainty and support 

for as long as your project is running 

• Custom-Tailored: You decide what you need and we offer you the best possible license terms 

Available services - predicting drug functionality and efficacy: accurate, reliable and fast 

• 3D Cell Culture Services from cell isolation to 3D organoid/spheroid model using the 3D CoSeedis TM technology. 

The 3-in-1 Plate & EZ-Seed-Plate 

APRICELL Biotechnology´s EZ-Seed is a culture insert (based 

on Agarose) for the production of tumor spheroids. 

The inserts are designed for a 12 well plate and can be used to 

produce 480 monosize spheroids per 12 well plate. The possible spheroid size is between 

300-600 µm (Cat#: APL-EZ-700). 

ABLE® Biott® Bioreactors 

ABLE BIOTT 3D disposable 30 ml bioreactors are designed with delta-shaped impellers and a conical shaft which offers 

ideal spheroid formation conditions for IPS cells. The bioreactors needs the Bioreactor System Controller and Motor as well 

as the Bioreactor Magnetic Stir System Base (30 and 100 ml). 

• 30 ml Bioreactor flask capacity, 5- to 10-fold cell expansion 

is possible (up to 50 million cells per flask) 

• 200-300 μm spheroids 

• Material (high-density polycarbonate) is compatible with 

iPS cell cultivation 

• Vessel interior is surface treated for biocompatibility 

• Polypropylene screw cap contains 0.2 μm nylon membrane 

• Sterile 

• Pack of 6 bioreactors 

This system can be used for various purposes such as: 

46


1. Tissue Engineering Research: Benchtop fluid bioreactors are employed in tissue engineering studies to simulate 

physiological conditions for the cultivation of cells and tissues. These systems allow researchers to investigate cell 

behavior, tissue development, and the impact of different factors on tissue growth and functionality. 

2. Regenerative Medicine: Fluid bioreactors are crucial for the development of regenerative medicine therapies. They 

provide controlled environments to support the growth and maturation of bio-engineered tissues, facilitating the 

creation of functional substitutes for damaged or diseased tissues. 

3. Drug Testing and Screening: These bioreactors are utilized for drug testing and screening purposes. The 3D cell 

cultures in fluid bioreactors more accurately mimic in vivo conditions compared to traditional 2D cell cultures, 

allowing for better predictions of drug responses and toxicity. 

4. Vascularization Studies: To create functional and viable tissues, vascularization is a critical factor. Fluid bioreactors 

enable researchers to study and optimize the vascularization process within bio-engineered tissues, improving the 

overall success of tissue transplantation. 

5. Stem Cell Expansion: Benchtop fluid bioreactors are used for the expansion of stem cells, providing a controlled 

environment to support the proliferation and differentiation of stem cells into specific cell lineages required for 

tissue engineering applications. 

6. Bioprocess Development: These bioreactors play a role in developing and optimizing bioprocesses for large-scale 

production of bio-engineered tissues. Understanding the requirements for scale-up and maintaining a consistent 

and controlled environment is crucial for successful tissue production. 

7. Muscle and Skeletal Tissue Engineering: Researchers use fluid bioreactors to create bio-engineered muscle and 

skeletal tissues. The controlled fluid flow and nutrient supply in these systems contribute to the development of 

tissues with improved structural and functional properties. 

8. Cartilage Regeneration: Benchtop fluid bioreactors are employed in the development of bio-engineered cartilage 

tissues. The controlled mechanical forces and nutrient supply within the bioreactor contribute to the formation of 

cartilage structures with desired properties. 

9. Connective Tissue Engineering: The creation of connective tissues, such as tendons and ligaments, involves using 

fluid bioreactors to simulate the mechanical forces and biochemical cues needed for tissue maturation and 

functionality. 

EZSPHERE TM Multi-Well Plates and Dishes (AGC ® ) 

EZSPHERE multi-well plates and dishes (AGC ® ) are coated with a cell/protein repellent (SP polymer) and made from 

polystyrene plastic. Unique features are the micro-wells laser etched to the bottom of the plates or dishes. EZSPHERE is 

used to generate high numbers of spheroids. The spheroid size is limited by the dimensions of the wells. 

Available are dishes from 35 – 100 mm, 6 and 96 well plates. 

47


3D Scaffold-based 

Organotypic Scaffolds - only available on demand 

Our partner Xylyx Bio has developed TissueSpec ® ECM Scaffolds based on ECM proteins isolated from different porcine 

organs. 

Human organotypic scaffolds are available only on demand. 

Pic: The TissueSpec ® Matrix 

Scaffolds retain the natural 

3D structure, biomechanics 

and topography of native 

porcine tissues. The 

scaffolds contain tissuespecific 

factors and 

architecture. 

What are the features of TissueSpec ® Matrix Scaffold? 

• 3D acellular native matrix construct 

• Derived from normal porcine tissue 

• Preserves native tissue architecture 

• Can be used in-vitro as well in-vivo 

• Compatible with fluorescence & light microscopy 

• Customized in size 


What type of TissueSpec ® Matrix Scaffolds are available on demand? 

• Bone • Cartilage • Heart 

• Intestine • Kidney • Liver 

• Lung • Skin 

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SEEDEZ TM 

What is SEEDEZ TM ? 

Our partner Lena Biosciences offers a robust 3D cell culture system for routine, predictive testing in vitro. SEEDEZ TM is a 

three-dimensional cell culture scaffold made of randomly oriented, inert glass microfibers. The diameter of microfibers is 

carefully optimized for long-term cell growth and repeat-dose drug testing. The microfibers are arranged in such a way that 

creates tiny pores for nutrients, gases, and metabolites to pass through the scaffold allowing normal cell functions. 

With a simple workflow and consistent composition, SEEDEZ TM can be used with all cell types. SEEDEZ TM enables the cells 

to utilize their cadherin (cell-to-cell connections) and integrin (cell-to-ECM) receptors which are vital for biomimetic 

functions of the engineered tissue. 

Unlike soft hydrogel-based scaffolds SEEDEZ TM does not peel off the culture dish or get drawn into the pipette tip. When 

compared to less porous and more rigid scaffolds, cells permeate SEEDEZ TM readily. 

How it works! 

At a glance: 

• Supports most cell types 

• Supports most ECMs 

• Consistent 3D cell distribution 

• Consistent cultures 

• Long-term culture (2 months+) 

• In-well assaying 

• Assay multiplexing 

• Fluorescence imaging 

• Easy to transfer from dish-to-dish 

• Cells can be recovered 

• Custom diameters upon request 

Applications: 

• Culture complex 3D cell culture and co-culture models long-term; 

• Small molecule drug testing (drug metabolism, toxicity etc.); 

• Testing on-target efficacy/ off-target toxicity of antibody-drugconjugates 

& multi-specific antibodies. 

SEEDEZ TM 

So easy to use! 

Just unwrap product, add cell suspension in appropriate medium at optimized seeding density and place in incubator to 

culture cells in 3D 

Is SEEDEZ TM compatible with other assays? 

3D tissues grown in SEEDEZ TM can be studied using a variety of standard molecular and cellular techniques. Cells can be 

harvested by using passaging solutions (e.g. trypsin or Accutase. DNA/RNA and proteins can be isolated direct out of the 

scaffolds. 

49


Alvetex® 

What is Alvetex®? It is a highly porous polystyrene scaffold designed for 3D cell culture. Cells grown in 

Alvetex possess a natural tissue-like structure that enables them to function in a more physiologically 

relevant manner. 

Alvetex 3D cell culture enables cells maintain their in vivo morphology, behavior and responsiveness 

within an in vitro model system. 

Alvetex® is a unique cellular environnement 

Manufactured to the highest standards of consistency each disc is engineered to a thickness of just 200 microns with pore 

sizes of 36-40 microns. No cell is ever further than 100 microns from the nutrient source enabling easily exchange of 

nutrients, gases and waste products by passive diffusion across short distances. Say goodbye to risks of foreign unknown 

materials such as proteins or cytokines of animal origin – unlike conventional materials used in cell culture Alvetex® is made 

from polystyrene and is completely inert. 

Advantage: Alvetex® has been designed for simple and routine use. It uses conventional cell culture plasticware. Therefore, 

any cell biologist can get into Alvetex 3D cell culture. Alvetex® is very easy to use! Just unwrap product, prepare scaffold, 

add cell suspension in appropriate medium at optimized seeding density and place in incubator to culture cells in 3D. 

Is Alvetex ® 3D cell culture compatible with other assays? 

In vitro derived 3D tissues grown on Alvetex ® can be studied using a variety of standard molecular and cellular techniques: 

• Tissue processing, fixation, embedding and sectioning 

• Histological staining, in situ hybridization 

• Bright-field microscopy and photographic imaging 

• Electron microscopy – both SEM and TEM 

• Cryostat Sectioning 

• Immunocytochemistry 

• Fluorescence microscopy, confocal, laser capture 

• Isolation of viable cells for passaging 

• Flow Cytometry and Cytospinning 

• Extraction of nucleic acid and total protein 

• Biochemical assays 

Application: A huge number of applications already exist. Just check www.reinnervate.com for further information. 

50


SpheroSeev 

SpheroSeev & HydroSeev are made of recombinant bio-polymer with the strength and elasticity of spidersilk which 

provides mechanical support and ECM-like environment to cell for spheroid formation and in hydrogels. SpheroSeev is a 

ready-to-use solution for spheroid formation. Spheroids containing SpheroSeev showing an increased viability, 

functionality and is suitable for long-term spheroid cultures. HydroSeev is a special formulation to be added to hydrogels 

for a better performance and stability. Just contact us for protocols to improve your alligate and/or Matrigel® (Corning) 

hydrogels. 

Advantages of using SpheroSeev: 

• Forms lager spheroids 

• Increases cell stability and viability 

• Enhances proliferation 

• Enables spontaneous spheroid formation 

Simply add SpheroSeev fibers to the 

cells and mix well prior to seeding in a 

ultra-low attachment plate. 

Fibers will integrate into the spheroids, 

improving viability and functionality of 

the cells. 

Adipose derived mesenchymal 

stem/stroma cells (MSCs) cultured as 

spheroids with SpheroSeev 

demonstrate a higher viability than 

cultured without SpheroSeev. (Greenviable 

cells, red – dead cells). 

51


3D Hydrogel-based 

Organotypic Hydrogels - TissueSpec® Matrix Hydrogel 

TissueSpec ® Hydrogel Kits are acellular matrix products derived from a 

variety of porcine organs and tissues. Versatile 

in format, hydrogel matrix kits offer the matrix components present in 

natural tissues and recreates a physiological substrate for 3D cell culture. 

TissueSpec® Hydrogel Kits have been shown to enhance cell attachment, 

growth, and function in stem cell research. These 

kits are designed for easy application in cell culture and provide structural, 

mechanical, and compositional cues of the native tissue 

microenvironment. 

TissueSpec ® Liver Hydrogel contains liver specific matrix components. 

• Compatible with standard cell culture programs 

• Lot to lot Consistency 

• Easy to Use 

Hydrogel can be applied as a thin gel. Liver cells migrate into 

hydrogel, enabling migration and invasion assays. 

Low-density lipoprotein uptake 

TissueSpec® Liver Hydrogel. liver cells show higher expression of LDL 

receptor and secretion of fibrinogen. pics: Xylyx Bio 

For optimal results: Combine Microvascular Endothelial Cell Growth Medium Kit enhanced (PB-MH 100-4099) with 

PELOBiotech-Cells. Ask for our human and animal cell list. 

Natural Hydrogels – Collagen & Fibronectin 

MatriMix 

The first MatriMix product, MatriMix (511), is composed of fibrillar collagen, recombinant human laminin-511 E8 

fragment, and hyaluronic acid. The types of collagen, isoforms of laminin, and the concentration of each component have 

been customized to provide the optimal extracellular environment for your target cells. Gelation takes advantage of 

collagen's ability to form fibers at a physiological temperature and solvent environment. 

52 

Figure 1 Cells derived from 

tissues during mouse 

development were cultured 

in 3D using MatriMix. Cell 

organization occurred by day 

7 of culture.


Collagen based - Human/Rat/Bovine 

We offer bovine & rat type I collagen in different concentrations (3-8 mg). 

Human platelet lysate based photopolymerizable hydrogels 

Call us for more information (+49 89 517 286 59-0) 

3D ready Atelocollagen 

Stable three-dimensional culture (3D culture) can be performed easily just by adding your cells to atelocollagen. 3D Ready 

Atelocollagen is premixed with DMEM low or high glucose and can be used at once. You only need to warm the 

atelocollagen up with your cells. 

Atelocollagen is produced from Bovine dermis and contains collagen type I (95%) and type III and type IV (approx. 5%). 

You can use the hydrogels to culture cells within the collagen gel or on top of the hydrogel. You can easily set up cocultures 

by adding one cell type into the hydrogel and the second cell type will be 

cultured on top. 

Features: 

• Ready-to-use collagen solution for 3D cell culture 

• Premixed with DMEM, low or high glucose 

• Easy to handle: just mix with cells and warm up for gel-formation 

• Low cell sedimentation during gel formation 

3D Models Ready-to use 

Scilia Extragel Matrix Products 

Scilia Extragel Matrix is a reconstituted hydrogel formed by basement membrane components which are extracted from 

mouse tumor tissues. The hydrogel is rich in extracellular matrix proteins and is mainly composed of laminin, collagen IV 

and heparan sulfate proteoglycans. Various growth factors (EGF, PDGF, HGF, FGF-2, TGF-ß and IGF are also part of the 

hydrogel. 


Key Benefits: 

• 3D cell culture - High Safety (tested negative for LDEV) 

• Angiogenesis - Lot-to-Lot Consistency 

• Stem cell maintenance - High Compatibility 

• Stem cell differentiation - Ample Production 

• In vivo xenograft generation 

Products available as standard hydrogel (8-12 mg/ml), as high concentration (18-22 mg/ml), with reduced growth 

factors and hESC qualified. All media are also available phenol-red-free. 

Figure 2: Human bile duct organoids in Scilia 

Extragel Matrix. Organoids are imaged after 

being stained with DAPI (nucleus-blue), anti- 

ZO-1 (tight-junction protein) and Alexa Fluor 

647 Phalloidin (cytoskeleton protein F-catin). 

Pic: Beijing Megarobo Technologies 

53


HydroSeev 

HydroSeev is a hydrogel supplement to overcome disadvantages of hydrogels like low stability and mechanical properties. 

HydroSeev is a biopolymer providing mechanical support to hydrogels like Alginate, Matrigel ® or Silica Extragel Matrix. 

Just adding 0,02% HydroSeev will improve viscosity, promotes a better porosity and mechanical stability. The distribution 

of cells within hydrogels is improved as well. 

Addition of 0.02% HydroSeev to cell cultures improves: A. Cell distribution in alginate (3.5%) hydrogel (green - HydroSeev, blue- cell 

clusters). B. Hydrogel viscosity is improved upon addition of HydroSeev. C. Mechanical properties of cell-seeded alginate hydrogel are 

significantly improved when HydroSeev is added (left). Stress-strain curve of compressed cell-seeded alginatehydrogel (right). Pic: 

SEEVIX 

Phenodrive 

Phenodrive, introduced by our partner Tissue Click, represents a revolutionary class of specialized substrates meticulously 

designed for organotypic tissue cultures and organoids. This synthetic biomaterial serves as a versatile platform for 

culturing and testing various cell phenotypes, manifesting as spheroids and co-culture models. Its design closely mimics 

tissue microenvironments, encompassing stem cell niches, epithelia, cancer masses, hepatocyte spheroids, and hypoxic 

conditions. Phenodrive's biomimetics seamlessly adapt to both 2D and 3D culture conditions, effortlessly accommodating 

commonly used plasticware. 

Key Features: 

• Composed of hyperbranched molecules presenting a range of bioligands. 

• Ordered presentation of cell-binding molecules at high density. 

• Suitable for 2D and 3D culture conditions. 

Cell Phenotypes: 

Individual ECMs are tailored for various cell phenotypes, including Stem Cells, Endothelial, Neural, Epithelial, Osteoblasts, 

Chondrocytes, Hepatocytes, Fibroblasts, Keratinocytes, Pancreatic beta cells, and Cancer cells. Different formulations 

cater to specific cell types. 

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• PhenoDrive-Y induces the formation of human bone marrow stromal cells (MSC) spheroids and angiogenic 

sprouting of endothelial cells. 

• PhenoDrive-I promotes the differentiation of induced pluripotent stem cells (iPSC) in brain cortical neuron 

networks and facilitates axonal growth in various neural cells. 

• PhenoDrive-R is suitable for most tissue cell phenotypes and supports the adhesion of 

monocytes/macrophages, mimicking their location in connective tissues. 

• PhenoDrive-PS induces osteoblast differentiation and is effective for MSC differentiation into osteoblasts. 

• PhenoDrive-U is versatile for most tissue cells and ensures adhesion through weak interactions with the cell 

glycocalyx, facilitating spheroid formation. 

• PhenoDrive-Q continuously consumes oxygen, creating localized low oxygen tension, and is recommended for 

cancer cell micro-masses and chondrocytes induction. 

User-Friendly and Flexible: 

• Provided as lyophilized powder. 

• Easily reconstituted in ethanol or aqueous media. 

• Compatible with various plasticware and 3D scaffolds. 

• Gamma- and UV-stable, maintaining optical and physicochemical properties. 

3-in-1 Plate 

Introducing the 3-in-1 Plate, a revolutionary hydrogel insert 

seamlessly designed to fit within each well of a standard sixwell 

plate. This innovative insert offers a comprehensive platform for spheroid/organoid 

formation, extracellular matrix integration, and drug testing, all in one. The hydrogel 

comes pre-inserted within each well, with each insert featuring four quadrants capable of 

accommodating six spheroids/organoids per quadrant, enabling a total of 144 formations 

per plate. Notably, the quadrants are independent, allowing researchers to run four 

different experimental conditions concurrently on a single insert. The hydrogel itself is 

non-cell-adhesive, offering numerous benefits and ensuring disturbance-free experimentation. The 3-in-1 Plate 

revolutionizes spheroid/organoid culture, drug testing, and downstream analysis, all while maintaining optimal 

experimental consistency and reducing cell requirements. Explore the advantages of this groundbreaking platform below: 

• Integrated Platform: Cultivate, embed in an extracellular matrix, test drugs, and perform downstream analysis 

without the need to remove spheroids/organoids from the platform. 

• Consistent Results: Low spheroid/organoid size variance enhances experimental consistency. 

• Isolated Conditions: One spheroid/organoid per microwell eliminates the possibility of inter-model crosstalk, 

ensuring accurate and isolated experimental conditions. 

• Disturbance-Free Media Changes: Safely change media without 

the risk of aspirating or disrupting spheroids. 

• Reduced Cell Requirements: Low cell requirements reduce the 

necessity to passage cells before experimentation. 

• Compatibility with Imaging Techniques: The platform 

compatible with in situ immunostaining and imaging. 

• Comprehensive IHC Analysis: Researchers can perform all steps 

of in situ IHC analysis without removing spheroids from the platform, from dehydration to wax embedding 

and slicing. 

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3D Skin Model 

What is CellApplications’ 3D Skin Model? 

It is a highly physiological, three-dimensional cellular system of Human Epidermal Keratinocytes (HEK) for in vitro studies, 

offering an excellent cellular system to examine aspects of epithelial function and disease, particularly those related to skin 

biology and toxicology. 

Advantage: Basic science and pharmaceutical researchers use the 3D Skin Model to replace animal tests for the assessment 

of skin irritation due to chemicals, skin-cream- based drugs and cosmetics. 

Application: CAI's Skin Model can also help avoid misclassification of chemicals and skin corrosion observed in animal 

systems (1, 2). Other applications include phototoxicity, percutaneous absorption & penetration, wound healing and 

metabolism. The cells are grown and differentiated into a stratified squamous epithelium on PCF inserts with a liquid/air 

interface. Contact us for more information. 

1) Eun & Nam, Exog Dermatol, 2:1–5 (2003) 

2) York et al., Contact Dermatitis, 34:204 (1996 

A. Depiction of Human Skin. 

B. Human Epidermal Keratinocytes (HEK) at ~80% confluency. 

C. Crystal Violet Staining. 

D. Hematoxylin & Eosin Staining of HEK differentiated into stratified squamous 

epithelium after 14 days. 

3D Airway Model 

What is the 3D Airway Model? 

It is a highly physiological, three-dimensional cellular system of Human Bronchial Epithelial Cells (HBEpC). 

Application: It is used for in vitro examination of epithelial function and disease, including airway infections, tissue repair 

mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, 

inflammation, pulmonary diseases and smoking. The cells are grown and differentiated into a pseudostriated epithelium 

on PCF inserts with a liquid/air interface. 

Fig A. Depiction of Human Airway. 

Fig C. Crystal Violet Staining. 

F i g D. Hematoxylin & Eosin Staining of HBEpC differentiated into 

pseudostriated epithelium after 28 days. (pic: CA) 

F i g B. Human Bronchial Epithelial Cell (HBEpC). 

The 3-D airway model is available as 12 or 24 well do-it-yourself-kit. The cells will 

be delivered cryopreserved. 

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Midifluidic 

Midifluidic Chambers 

Together with our Italian partner IVTech we offer advanced cell 

culture systems for 3D Cell Cultures. The Midifluidic systems 

(Perfusion Chambers) in the classical 24 well format can be used to 

perform 3D dynamic in-vitro models, following the standard 

protocols. 

IVTech is a spin-off from the University of Pisa. It was founded in 

January 2014 by 4 bioengineers. They have over ten years’ 

experience in the design and development of innovative systems for 

in-vitro cell culture. 

We provide two different systems (LiveBox 1 & 2) with transparent 

glass bottoms which allow live imaging during static/dynamic 

culture. Both systems can be connected to build up multi organ 

systems. Of course, we offer starter kits which include also a pump 

system. 

LiveBox 1: dynamic cell cultures. LiveBox1 is a transparent chamber 

designed for inter-connected dynamic cell cultures. LB1 is featured 

with a flow inlet and outlet for the perfusion of cell culture media. 

LB1 

• single flow bioreactor 

• metabolic organs simulation 

LiveBox 2: for dynamic in-vitro-models of physiological barriers. 

LiveBox2 is transparent chamber, developed to model physiological 

barriers (e.g. lung and intestinal epithelium) in-vitro. LB2 is designed 

for inter-connected dynamic cell cultures and is equipped with two 

flow inlets and outlets, and a holder to house a porous membrane. 

• double flow bioreactor 

• physiological barriers simulation 

• equipped with a membrane 

LB2 

LiveFlow is a compact and low weight peristaltic pump, compatible with the incubator environment. We also offer 

LiveFlow PRO to automate your invitro experiment and LivePa a pressure modulator useful to introduce pathological 

conditions, thus being one step closer to human reality. 

• compact and low weight peristaltic pump 

• 4 Independent fluidic circuits 

• 2 removable heads 

Why Advanced Cell Culture Systems: 

• Human organ environment simulation 

• Multi-organ models 

• 3D and dynamic cell cultures 

• Real time monitoring 

LiveFlow - peristaltic pump compatible with the chambers 

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The integration of microfluidic systems with 3D cell cultures has enabled 

the study of angiogenesis, microcirculation, and vascular functions with 

high precision and control. These models have facilitated the elucidation 

of the roles of the microvasculature in health and disease, particularly in 

the context of angiogenesis and thrombosis. 

Microfluidic devices have been instrumental in culturing 3D cells under 

dynamic conditions, providing a platform for high-throughput drug 

testing on cancer spheroid models. The successful commercial application 

of 3D hepatocyte in vitro models for drug testing has been linked to the 

development of hepatocyte-supporting 3D microenvironments in 

microfluidic channels. Microfluidic platforms incorporated with 3D 

microscaffolds and submicron/nanoscale topographies have shown 

promise for 3D cell cultures, offering a potential model for various 

applications. 

Microfluidics has enabled dynamical screens with precise control over the 

concentration, timing, and duration of fluidic delivery, particularly in the 

context of drug screening on tumor organoids. By leveraging the small 

dimensions and laminar flow inherent in microfluidic systems, in vitro 

models have been developed to reproduce many features of the in vivo 

vascular microenvironment with fine spatial and temporal resolution. 

These models have provided valuable insights into the dynamics of singlecell 

immune responses and have facilitated the quantification of immune 

dynamics at a high content level. Furthermore, microscale in vitro 

physiological models of the liver have been developed using microfluidic 

systems, enabling predictive screens for drug metabolism, enzyme 

induction, and assessment of acute and chronic liver toxicity arising from 

exposure to drugs or environmental agents. 

MCF7 cells are seeded into LiveBox 1 

Multiorganoid testing with multiple chambers, 

(pics: IVTech) 

Physiological Models: 

• Cornea 

• Liver 

• BBB 

• Intestinal Organoids 

• Upper Bronchial Tubes 

Disease Models: 

• Rare disease: test of a novel approach 

• Breast Cancer 

• Diabetes Treatment i.e. Langerhans - islet 

transp. 

Multi Organ Model: 

• Study of Crosstalk between 

normal and cancer tissues 

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Blood-Brain-Barrier (BBB) system 

Realistic. Dynamic. Cell-Based Assays 

SynVivo offers a physiological, cell-based microchip platform that provides a morphologically and biologically realistic 

microenvironment allowing real-time study of cellular behavior, drug delivery and drug discovery. 

A Microfluidic Cell-based Assay Platform imprinted with real microvasculature morphology... 

…cultured with cells and studied under physiological flow to provide a more realistic in vitro assay. 

How it works... 

Use Microvascular Networks to replicate in vivo cell/particle adhesion 

and cell-cell or cell-particle interactions in an in vitro setting. 

Investigate effects of flow and morphology for drug development and 

cellular research. Obtain shear-adhesion maps and bifurcation vs. 

branch adhesion in single experiment. 

By incorporating natural tissue regions within the network topology, 

the co-culture networks allow study of cell and drug behavior at and 

across the interface between the vessel lumen and tissue space. The 

co-culture network constructs are available with several options for 

channel size, tissue region scaffolding, barrier design and 3D tumor 

models. Intended to mimic the formation of and transport across tight 

and gap junctions such as the blood-brain barrier (BBB) and other 

endothelial/tissue interfaces, the idealized co-culture constructs are 

available with several options for channel size, tissue chamber size 

and scaffolding, and barrier design. 

The Most Realistic In vitro Blood-Brain Barrier Model 

SynVivo’s Blood-Brain-Barrier (BBB) system recreates the in vivo microenvironment by emulating a histological slice of 

brain tissue cells in communication with endothelial cells across the BBB. SynVivo-BBB avoids limitations of traditional BBB 

models such as lack of physiological shear stress and real-time visualization capability. The SynVivo-BBB system serves as 

an exceptionally versatile platform, offering comprehensive investigation capabilities across various domains. This 

innovative system enables in-depth exploration of tight junction proteins, transporter proteins, drug permeability, 

inflammation, cell migration, omic changes, neurotoxicity, and neuro-oncology within the intricate framework of the 

blood-brain barrier (BBB). Its adaptability extends to diverse application areas, making it an invaluable tool for basic 

research, drug delivery optimization, drug discovery acceleration, and ADME/Tox assessment. Researchers benefit from a 

realistic environment to unravel BBB dynamics and phenomena, optimize drug delivery strategies, expedite drug discovery 

processes, and assess critical drug characteristics. The SynVivo-BBB system stands at the forefront of scientific 

advancements, fostering a deeper understanding of biological processes and contributing to breakthroughs in research and 

pharmaceutical development. 

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PerfusionPAL 

What is Perfusion? 

Perfusion describes the rate at which blood is supplied to a given organ 

in the body. We believe this crucial factor can play a role in cell culture 

methods in order to positively impact cell survival mechanisms in vitro 

as well as simulate real life growth conditions for your research. 

Perfusion delivers oxygen to regulate metabolic activity of cells and 

promotes dissipation of heat. Poor perfusion may lead to cell death, 

and on a larger scale tissue necrosis. Our technology can be adjusted 

to deliver the specific perfusion rate needed for your model. 

What is Perfused Organ Panel? 

The POP system is a lab technology that combines the 3D cell 

culturing capabilities of the SeedEZ scaffold with the advanced 

oxygenation of our Blood Substitute within a compact, perfused 

multi-well plate, PerfusionPal. The system is ideally suited for 

modeling interstitial perfusion that cells in most tissues experience in 

vivo. 

Our PerfusionPal plate is easily perfused with a single tube that is 

attached to the base of the plate. The tube is flexible and has a durable 

metal sheath that you can use to route the tubing between the gasket 

and the door of the incubator. At the other end of the tubing is a syringe 

pump, with room for 2 syringes to operate 2 PerfusionPal plates at 

once. 

The perfusion rate is set by the user and can be easily paused for 

alterations, or stopped for media changes. Pumping of this blood 

substitute acts as a "liquid piston" that introduces perfusion 

uniformly along the plate, without the need for separate pumps and 

tubing for each culture. 

pics. Lena Biosciences 

Applications include: 

• Small molecule drug testing: drug metabolism, on-target efficacy & off-target toxicity 

• Large molecule biopharmaceutical testing: interstitial delivery mimicking in vivo conditions, on-target efficacy/ off-target 

toxicity of antibody-drug-conjugates & multi-specific antibodies 

• Cancer research: simulate advanced, vascularized tumors with heterogeneous cell populations, extracellular matrix, & intratumor 

supply of drugs to more closely mimic a patient’s tumor at the time of treatment 

How does it work? 

Step 1: 

Add Blood Substitute to 

the bottom of the 

PerfusionPal tray 

Step 2: 

Insert middle component 

containing wells, and add 

media to each well 

Step 3: 

Seed cells into SeedEZ scaffold 

and place scaffolds into wells. 

Set perfusion rate on pump and begin 

perfusion 

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Benefits of using PerfusionPAL : 

• Superior oxygenation and gas exchange 

• Physiologically relevant interstitial perfusion 

• Long-term culturing, testing and analysis 

• In-well assaying (supernatants can be transferred to another plate for reads) 

• Time-course analyses by sampling secretome and metabolome from wells, using ordinary micropipette 

• Customizable perfusion rates 

• Reliable perfusion (there are no bubbles and no microchannels to clog) 

• Statistically independent wells 

• Zero dead volume in fluidics 

• Sterile and ready to use 

• Blood Substitute can be reused, and maintains purity 

• Blood Substitute is inert and immiscible with media, reagents, drugs etc. 

• Offers more precision and control compared to animal models 

GradientEZ 

GradientEZ is a qualitative cell migration and invasion assay that enables cells to migrate in three dimensions as 

they do in living tissues. It is straightforward to use and works like a piece of paper. Simply, pipet cells in the center 

and add test agents to petals or lobes to model physiologically relevant, gradient-dependent pathological 

conditions in 3D. Alternatively, add invasive cancer cells to the center and normal cells to lobes. Fluorescently labeled 

cells or cell populations added to the center or lobes may be imaged and analyzed in real time during or after cell 

migration studies using fluorescence microscopy. 

Suitable for both adherent and suspension cells. 

How does it work? 

Method 1: 

Migrating or invading cells, and 

different cell populations or test 

compounds may be added to 

the center and 6 petals or lobes 

Method 2: 

To test metastatic potential of cancer 

cells in vitro, add invasive cells to the 

center and normal cells (e.g. brain, 

lung, liver, bone, adrenal, etc.) to lobes 

and track invasion to begin to elucidate 

where they might 

metastasize in vivo. 

Benefits of using GradientEZ: 

• Closely mimics physiologically relevant in vivo conditions 

• Fits in any Petri dish, 6-well to 12-well plates & 6-well inserts 

• Test modulators of cell motility or invasion, in parallel, in one assay 

• Up to six different concentrations of the same agent can be tested in one well 


• Cell migration assays, cell invasion assays, chemo-invasion assays, chemotaxis assays 

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Essential Extras 

• Antibodies 

• Dyes 

• Proteins 

• TR-FRET Reagents 

o Europium chelates 

• Reagents 

• Tools 

• Sample Preparation Tools 

• Safety tools 

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Antibodies 

Antibodies are glycoproteins produced by plasma cells in response to foreign antigens that illicit an innate immune response. 

Due to recombination of the genes the variety of antibody responses is vast but highly specific, monoclonal antibodies are 

produced by clonally expanded by lymphocytes against a specific antigen and these have similar antigen binding site region 

binding affinity for their targets and identical downstream effects. These antibodies are often used in biochemical analysis, 

radioimmunotherapy, detection of various viruses and diseases. We offer various antibodies for detection of immune cells, 

proteins, epigenetic modifications, viruses, stemness and diseases. 

Amyloidosis is one such disease which is difficult to diagnose due to the heterogeneity of the amyloid proteins. The gold 

standard for amyloidosis diagnosis is tissue biopsy followed by Congo red staining and confirmation through characteristic 

apple-green birefringence under polarized light microscopy. However, biopsies may not always be feasible or accessible, 

and sampling errors can occur due to the focal nature of amyloid deposition. Additionally, immunohistochemistry, 

immunofluorescence, and electron microscopy can aid in subtype identification. 

However, these techniques have limitations in detecting small or oligomeric forms of amyloid fibrils. Currently amYmed is 

the only company that offers antibodies designed for detecting amyloidosis. The immunohistochemical classification of 

amyloid using the amY-kit antibodies is a key offering from amYmed. Their antibody panel consists of 10 tubes with 16 

antibodies, covering 98% of the most common amyloidosis. The accuracy of their classification method has been validated 

through extensive studies, blind comparative analyses, and collaborations with other institutes. 

Comparisons with mass spectrometry have further confirmed the reliability and quality of their approach. The advantages 

of immunohistochemical classification include its precision, simplicity, speed, and affordability. By utilizing this technique, 

healthcare professionals can quickly identify the correct amyloid type, even in the early stages of the disease, allowing for 

timely intervention and management. 

If you would like to know more about Amyloidosis then please take a look at our article on our website here. 

You can also check out articles by a leading researcher in the field of amyloidosis here. 

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Dyes 

You can find a wide range of dyes for microscopy that can be used to stain various 

cellular organelles, proteins, membranes, and nucleotides. 

There are live cell dyes available that allow for organelle visualization with organelleselective 

stains (e.g. ER, mitochondria, Golgi) or function assays like, e.g. live cell 

tracking, labeling, cell proliferation, or live dead assays. 

You may find a brief overview of our product range in the table below: 

Product 

NucleoSeeing 

CytoSeeing 

ERseeing 

LipiDye 

FAOBlue 

Thermoprobes 

Ap3 

PolyamineRED 

Application 

Live cell imaging, cell cycle studies, DNA damage assessment, environmental stress studies, drug 

screening, cell viability assays, cell sorting and isolation, cancer research, intracellular pH 

monitoring 

Our microscopy dyes provide live cell visualization without compromising cell viability. They 

allow tracking of cell division and migration, co-staining with other probes, and are suitable 

for FACS or flow cytometry analysis. 

A permanent endoplasmic reticulum (ER) staining dye is a versatile tool for cellular research 

and diagnostics. It aids in studying ER morphology, monitoring cellular health, assessing ERrelated 

diseases, evaluating drug effects, diagnosing conditions, and enabling precise cell 

sorting. 

Our specialized lipid detection dye is essential for imaging live and fixed cells, particularly for 

staining tiny lipid droplets smaller than 1 micrometer, a task often challenging for conventional 

dyes like Nile Red. 

This unique capability is invaluable in lipid metabolism and cell biology research, enabling 

detailed insights into cellular processes, lipid dynamics, and lipid-related disorders like obesity 

and diabetes. 

Our fatty acid oxidation detection dye simplifies live cell imaging and offers insight into singlecell 

metabolic activity. Its applications extend to NASH research, allowing for the assessment of 

liver cell lipid metabolism, as well as broader metabolic studies for treatments and disease states. 

This dye is ideal for real-time, dynamic metabolic observations and interventions in metabolic 

disorders. 

Our live polymeric thermometer dye provides real-time intracellular temperature insights for 

living cells, making it a valuable tool in fields like cancer research, neuroscience, and drug 

development. It allows researchers to study cellular responses to treatments and conditions, 

uncover temperature regulation mechanisms, and understand how temperature fluctuations 

impact cellular processes, all in a non-invasive and continuous manner. 

Our SHG detection dye is essential for enhancing true SHG signal detection and enabling 

multimodal imaging. It has applications in neuroscience for visualizing neural structures, in tissue 

engineering for studying extracellular matrix components and cell interactions, and in material 

science for analyzing material properties and molecular arrangements. This dye plays a crucial 

role in advancing research in these diverse fields. 

PolyamineRED is a powerful tool for the non-invasive, semi-quantitative detection of 

intracellular polyamines without the need for pre-treatment or cell lysis. This versatile dye finds 

applications in diverse fields, including cancer research, neurobiology, and drug development. 

It enables the study of polyamine levels and distribution within live cells, offering insights into 

cancer progression, neuronal function, and the evaluation of potential drug candidates targeting 

polyamine-related pathways. 

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Proteins 

Proteins, essential building blocks of life, are pivotal in diverse scientific applications. Their structural intricacies, unveiled 

through techniques like X-ray crystallography, inform drug design and targeted therapies. Serving as enzymatic catalysts, 

proteins drive biochemical reactions crucial for biotechnology, medicine, and environmental science. In cell signaling, 

proteins regulate physiological responses and are targets for therapeutic interventions, especially in diseases like cancer. 

Biotechnological advancements leverage proteins for synthesizing therapeutic agents and engineering novel functions in 

synthetic biology. Additionally, proteins are indispensable in diagnostics, with biomarkers aiding disease detection and 

monitoring. In essence, proteins are the linchpin of scientific progress, contributing to our understanding of life and 

propelling innovations across various disciplines. 

Recombinant Proteins 

Our extensive offering includes a diverse range of recombinant proteins targeting CD73, GPC3, Siglec-15, FGL1, 

and more. Available in formats like Fc-fusion, 6His-tag, and Fc-tag, these proteins are sourced from various 

entities—human, mice, rat, rhesus, rabbit, and even viruses like SARS-CoV-2. Biotinylated versions add flexibility 

to your research. Essential proteins like immunoglobulins and VEGFR further empower investigations across 

scientific domains. 

Human Proteins 

Recombinant human proteins play vital roles in medicine and biotechnology. Insulin manages diabetes, PDGFs aid 

wound healing, and growth factors like SCF, bFGF, and EGF contribute to cell proliferation and tissue 

regeneration. TGF-beta 1 regulates growth and differentiation, ensuring high-quality standards through cGMP. 

Proteins like GM-CSF stimulate white blood cell production, and cGMP-produced EPO is vital for treating anemia. 

Enzymes 

Anti-lysozyme, anti-cystatin C, and anti-gelsolin serve as valuable tools in research and diagnostics, detecting and 

quantifying specific proteins. Amylase detection kits (amY-Kit) assess amylase activity for insights into pancreatic 

and salivary gland function. The animal-free nature of these proteins ensures ethical production methods, setting 

them apart from cGMP-produced counterparts. 

Growth Factors 

Animal-free growth factors play pivotal roles in cell growth, proliferation, and differentiation. PDGFs aid wound 

healing, FLT-3L stimulates immune cell production, and M-CSF regulates macrophage development. Oncostatin 

M and TGF beta 3 find applications in inflammation, tissue regeneration, and embryonic development. VEGFs are 

key players in angiogenesis, RANK Ligand is essential for bone remodeling, and beta NGF is involved in the nervous 

system. Animal-free versions ensure ethical and controlled applications. 

Chemokines 

Among the diverse offerings, chemokines play a crucial role in immune responses and inflammation. Chemokines 

guide the movement of immune cells to specific sites in the body. Examples include CCL2 (Monocyte 

Chemoattractant Protein-1) and CXCL8 (Interleukin-8), which attract monocytes and neutrophils, respectively. 

These chemokines are valuable tools in research, particularly in studying diseases involving immune cell 

recruitment. Their application extends to diagnostics, aiding in the detection and quantification of specific proteins. 

The ethical and controlled production of these chemokines distinguishes them from cGMP-produced counterparts. 

Cytokines 

Cytokines have diverse applications in immunotherapy, cancer treatment, and research. ILs modulate immune 

responses, with IL-2 used in cancer immunotherapy. TNF-alpha is employed in cancer treatment and autoimmune 

65


diseases. G-CSF is crucial for recovering white blood cell counts after chemotherapy. IFNs like interferon-beta have 

antiviral and anti-inflammatory applications. Animal-free versions cater to applications where the absence of 

animal-derived components is critical, ensuring ethical and controlled use. This distinguishes them from cGMPproduced 

cytokines. Reach out to us for specific details tailored to meet your research needs. 

TR-FRET 

Time-Resolved Fluorescence Resonance Energy Transfer 

(TR-FRET) is a powerful and versatile molecular assay 

technique used in biological research and drug discovery. 

TR-FRET allows scientists to study molecular interactions 

and events within cells or biochemical reactions with high 

sensitivity and precision. It is particularly valuable for 

measuring protein-protein interactions, receptor-ligand 

binding, and various other biomolecular processes. 

TR-FRET relies on the principles of fluorescence resonance 

energy transfer (FRET), in which energy is transferred from 

one fluorophore (the donor) to another (the acceptor) 

when they are in close proximity, typically within 10-100 

angstroms. The key distinction in TR-FRET is the measurement of the time delay between excitation and emission, which 

eliminates short-lived background fluorescence and significantly enhances signal-to-noise ratios. 

To perform TR-FRET assays, researchers require specific reagents: 

• Donor and Acceptor Fluorophores: These are the fluorescent molecules that emit light when excited by an energy 

source. The donor fluorophore, typically a lanthanide chelate (e.g., Europium or Terbium), is excited by a pulsed 

light source, and its emission is transferred to the acceptor fluorophore (e.g., XL665 or APC), resulting in a FRET 

signal. 

• Target Molecules: These are the biological components under investigation, such as proteins, nucleic acids, or 

small molecules, labeled with the donor or acceptor fluorophores. The choice of labeling strategy depends on the 

specific experiment and the accessibility of the target sites. 

• Buffer Solutions: Appropriate buffer solutions are used to maintain physiological conditions and optimize the 

stability of the labeled molecules. 

• Time-Resolved Detection System: This includes a fluorescence plate reader or a dedicated TR-FRET reader 

equipped with the capability to measure the time delay between excitation and emission of the fluorophores. 

TR-FRET has revolutionized the field of molecular biology and drug discovery by enabling the study of complex cellular 

processes in a high-throughput and sensitive manner. Researchers can use TR-FRET to assess biomolecular interactions, 

screen for potential drug candidates, and gain insights into cellular pathways and mechanisms with exceptional accuracy 

and efficiency. 

Europium chelates 

Europium chelates are a class of luminescent compounds widely used in various applications, particularly in the field of 

fluorescence-based assays and diagnostics. These chelates consist of europium ions (Eu3+) bound to organic chelating 

ligands, which enhance their stability and luminescent properties. Europium ions are known for their unique photophysical 

properties, including a long fluorescence lifetime, narrow emission bands, and resistance to photobleaching. These features 

66


make europium chelates ideal for time-resolved fluorescence assays, such as Time-Resolved Fluorescence Resonance Energy 

Transfer (TR-FRET), where the time delay between excitation and emission is crucial. 

We offer: 

• Several assay kits, including bFGF aptamer and VEGF aptamer assay kits, utilize Europium chelates for enhanced 

sensitivity. 

• QuickAllAssay offers a range of activated Eu-W1024 and Eu-W8044 chelates, along with labeling kits. 

• A variety of molecules, such as 2'/3'-ADP, 2'/3'-ATP, cAMP, cGMP, GDP 2'/3', 2'/3'-GTP, and 8-GTP, can be 

labeled with Europium for detection. 

• GTP-specific antibody fragments are available, as well as streptavidin labeled with Europium. 

• Custom assay development and labeling services, including antibodies and small molecules, are also offered. 

Product purification 

Elevate your purification processes with our extensive range of laboratory products meticulously crafted for various 

molecular biology and protein purification applications. Whether you're working on RNA or DNA purification, plant DNA 

extraction, or IMAC metal affinity purification, we have the ideal solutions to meet your needs. Our product line also 

includes an array of essential biochemical reagents and magnetic particles designed for efficient purification, making it easier 

to achieve your research goals. Plus, with a range of tip boxes and Quick Pick tips, you can optimize your purification 

workflow to enhance efficiency and precision. Discover how our purification-focused products can empower your 

laboratory work and help you achieve outstanding results. 

• Various kits and reagents are available for automated nucleic acid and protein purification, including total RNA and 

genomic DNA kits. 

• QuickPick series includes different kits for RNA and DNA extraction, with varying preps and buffer volumes. 

• Specialized kits are available for plant DNA extraction and IMAC metal affinity purification. 

• A range of buffers and magnetic particles are offered for RNA and DNA purification. 

• Various biochemical reagents like Streptavidin, Protein A, Protein G, and functionalized magnetic particles (Hydroxyl, 

Amine, Carboxyl, Chymotrypsin, HILIC, NTA, Protein A, Streptavidin, TiO2, Ti-IMAC, Trypsin, ZrO2, and Zr-IMAC) 

are also provided. 

ReSyn 

ReSyn ® 's ultra-capacity high performance magnetic microparticles for MS Sample preparation and bioseparation 

workflows will improve the reproducibility and automation. The extensive range of biochemical reagents, including 

Streptavidin, Protein A, Protein G, and a diverse selection of functionalized magnetic particles, opens up a world of 

possibilities in various scientific applications. Streptavidin, known for its high binding affinity to biotin, is invaluable in 

assays and detection methods, facilitating the isolation and purification of biotinylated molecules. Protein A and Protein 

G are crucial for antibody purification, enabling researchers to obtain highly pure antibodies for use in diagnostics and 

research. 

The functionalized magnetic particles offered by ReSyn, spanning Hydroxyl, Amine, Carboxyl, Chymotrypsin, HILIC, 

NTA, Protein A, Streptavidin, TiO2, Ti-IMAC, Trypsin, ZrO2, and Zr-IMAC, cater to diverse applications. Hydroxyl, 

Amine, and Carboxyl groups provide versatile surface modifications for specific binding interactions, while 

Chymotrypsin and Trypsin-functionalized particles are essential tools for protein digestion in proteomics research. 

Additionally, the inclusion of specific metal and resin-functionalized particles such as TiO2, Ti-IMAC, ZrO2, and Zr- 

IMAC expands the utility to phosphopeptide enrichment and metal affinity chromatography. NTA-functionalized 

particles are ideal for immobilizing polyhistidine-tagged proteins in affinity chromatography. ReSyn crafts custom 

particles for your unique purification needs, tailor your experiments with precision and efficiency. 

Whether it's biomolecule purification, protein digestion, or chromatographic separations, ReSyn's comprehensive range 

of reagents and functionalized magnetic particles provides researchers with the tools they need to streamline and 

enhance their experiments across a wide spectrum of life sciences and biotechnology applications. 

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Molecular biology tools 

Unlock the potential of your molecular biology and PCR experiments with our wide range of reagents. These versatile tools 

are tailored to support various applications, including cDNA synthesis, multiplex PCR, RT-PCR, and qPCR. Whether you 

need to amplify DNA, generate complementary DNA strands, or perform quantitative PCR, our selection of reagents 

provides the essential components to ensure the success of your molecular biology research. Explore our offerings to find 

the right solutions for your specific applications and take your experiments to the next level. take your experiments to the 

next level. 

Category 

DNA Polymerases 

cDNA Synthesis and 

Master Mixes 

dNTPs 

qPCR and Probe 

Master Mixes 

Compounds 

DNA Free-Taq DNA Polymerase, DNA Free-DuoTaq Polymerase, DNA Free-DuoHotTaq 

Polymerase, DNA Free-HotTaq Polymerase, DNA Free-Pfu DNA Polymerase, DNA Free- 

Quantum Pfu DNA Polymerase 

CellScript All-in-One 5X 1st cDNA strand synthesis Master Mix, 2X DNA Free-Multiplex 

PCR Master Mix, 2X DNA Free-HotTaq Mastermix, 2X DNA Free-Multiplex PCR Master 

Mix, 2X DNA Free-Pfu Mastermix, 2X DNA Free-Quantum Pfu Mastermix, 2X DNA Free- 

Taq Mastermix, CellScript one step 2X RT-PCR Master Mix, CellScript one step RT- 

QGreen 2X qPCR Master Mix, CellScript one step RT-QPlex 2X Probe Master Mix, 

CellScript one step 2X RT-PCR PreMix 

DNA Free dNTPs, 10mM (each 2.5mM), DNA Free dNTPs, 40mM (each 10mM) 

QGreen 2X qPCR Master Mix (no ROX), QGreen 2X qPCR Master Mix (High ROX), 

QGreen 2X qPCR Master Mix (Low ROX), QPlex 2X Probe Master Mix 

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Small Molecules 

The products listed encompass a diverse array of chemical compounds with wide-ranging applications in life science 

research. They play pivotal roles in studying signal transduction pathways, stem cell behavior, epigenetics, receptor 

biology, and cellular processes. These compounds are essential tools for gaining insights into biology, identifying 

therapeutic targets, and advancing our understanding of diseases, making them indispensable in the fields of regenerative 

medicine, developmental biology, and cancer research. 

Category 

Compounds 

Kinase Inhibitors 

Y-27632 (Rock-Inhibitor), Gö 6983, BI-D1870, CHIR99021 , SU5402 , TG101348 , WH-4- 

023 , BIRB796 , PTK787 , JNJ-10198409 , PP1 , SB590885 , Staurosporine 

Stem Cell 

Modulators 

Stemolecule ALK5 Inhibitor , Stemolecule Thiazovivin , Stemolecule Wnt Inhibitors (IWP-2, 

IWP-3, IWP-4) , Stemolecule A83-01 , Stemolecule LDN-193189 , Stemolecule KAAD- 

Cyclopamine , Stemolecule All-Trans Retinoic Acid , Stemolecule Cyclopamine , Stemolecule 

Dorsomorphin , Stemolecule XAV939 , Stemolecule ec23 , Stemolecule Valproic Acid , 

Stemolecule Sodium Butyrate 

Epigenetic 


Trichostatin A , SAHA , HDAC Inhibitors (Sorafenib, Vorinostat) , Decitabine 

Receptor 


Plerixafor , VEGFR Inhibitors (Sunitinib, Vandetanib) , Sorafenib , Rosiglitazone - Ruxolitinib , 

RAR Agonist (All-Trans Retinoic Acid) 

Cell Signaling 

and Cell Cycle 

Inhibitors 

GSK3 Inhibitors (CHIR99021) , MEK Inhibitors (PD0325901) , PI3K Inhibitors (GDC-0941, 

LY294002) Wnt Pathway Modulators (Wnt Inhibitors) mTOR Inhibitors (NVP-BEZ235) 

Kinase inhibitors, exemplified by Y-27632 and Gö 6983, are pivotal tools for exploring signal transduction pathways, aiding 

researchers in unraveling cellular responses and pinpointing potential therapeutic targets across various diseases. Stem cell 

modulators, including Wnt inhibitors and RAR agonists, are essential for manipulating stem cell behavior and differentiation, 

thus propelling advances in regenerative medicine and developmental biology studies. Epigenetic modulators like 

Trichostatin A and Decitabine are invaluable for epigenetic research, shedding light on gene regulation intricacies and 

potential cancer therapies. Receptor modulators, such as VEGFR inhibitors and Plerixafor, drive investigations into receptor 

biology and angiogenesis, expanding our comprehension of cellular communication. Furthermore, cell signaling and cell 

cycle inhibitors like CHIR99021 and PD0325901 serve as indispensable tools for probing various cellular processes, from 

regulating the cell cycle to deciphering intracellular signaling pathways. These products cater to various research needs, 

including the study of kinase signaling, stem cell biology, epigenetics, receptor biology, and cell cycle regulation, 

providing researchers with valuable tools for their experiments and investigations. 

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Assays 

Angiogenesis Assays 

Cell based Assays 

Cell Purity Kits 

Chimerism Assays 

Metallo Quantification Assays 

Metastasis Assays 

TR-FRET Kits 

Angiogenesis Assays 

Angiogenesis assays are crucial tools for studying the formation of new blood vessels from existing ones. They have broad 

applications in biomedical research, helping scientists investigate angiogenesis mechanisms, test potential therapies, and 

understand its role in diseases like cancer, cardiovascular conditions, and wound healing. These assays measure endothelial 

cell activities, aiding in the development of treatments targeting angiogenesis-related disorders. These products are a series 

of in vitro angiogenesis tracing kits designed for specific microvascular regions in the human body, including the brain, 

glomerular, lymphatic, pancreatic, and retinal areas. They provide researchers with tools to study and trace angiogenesis 

processes in these distinct microvascular environments. 

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Cell-based Assays (ECM Assays) 

Cell biology assays are pivotal in the field of life sciences, enabling researchers to investigate and understand various cellular 

processes. Among the essential tools are the Cyto-X Solution, available in both full kits and sample sizes, which provide a 

colorimetric method for precise cell counting. 

For researchers interested in the extracellular matrix (ECM) and its influence on cell behavior, the MicroMatrix 36 and 

MicroMatrix 36-4 Pack are ideal for testing the most suitable ECM for specific cell types. The screenMatrix in a 96-well 

format facilitates high-throughput screening, making it a valuable tool for cell biology studies. These assays collectively 

contribute to our understanding of cellular functions and the optimization of cell culture conditions. 

Cell Purity Kits 

Accumol's MTBN Purity Assessment Kit is a versatile tool for detecting Monocyte, T-Cell, B-Cell, and Neutrophil mRNA in 

genomic DNA samples isolated from sorted cells. This kit is specifically designed to quantify lineage-specific RNAs that are 

co-purified during standard DNA isolation procedures. After reverse transcription and PCR amplification, the resulting 

products can be analyzed on a sequencer or traditional gels. This test is not influenced by the presence of beads or antibodies 

on sorted cells' surfaces and can be applied regardless of the sorting method used. Furthermore, as it assesses purity at the 

nucleic acid level, it allows for delayed analysis, even months after cell sorting. 

Chimerism Assays 

Checking chimerism, the presence of two or more genetically distinct cell populations within an individual, is crucial for a 

variety of applications in the field of medicine and research. The provided Non-B Genomic Detection Kits and Non-T 

Genomic Detection Kits, as well as the qPCR Non-Myeloid Genomic Detection Kit, offer powerful tools for this purpose. 

Chimerism testing plays a vital role in clinical contexts, such as post-transplant monitoring, where assessing the proportion 

of donor and recipient cells is essential to evaluate the success of organ or bone marrow transplants. It is also instrumental 

in identifying and tracking the presence of foreign cells in cases of graft-versus-host disease and determining the 

effectiveness of therapeutic interventions. 

In forensic science, chimerism testing is valuable for solving complex cases by distinguishing between the DNA profiles of 

individuals. In research, chimerism analysis helps scientists understand developmental processes, genetic mosaicism, and 

the dynamics of cell populations within organisms. 

These detection kits are indispensable in these applications, providing accurate and reliable methods to assess chimerism, 

ultimately contributing to improved patient care, the advancement of forensic investigations, and a deeper understanding 

of biological mechanisms. 

Metallo-quantification Assays 

Metallo quantification assays, such as the Copper, Iron, Magnesium, and Zinc Assay kits provided, are essential tools in 

analytical chemistry and various scientific disciplines. These kits offer precise methods for quantifying the concentration of 

specific metal ions in various samples, employing different detection techniques like the 3,5-DiBr-PAESA, Ferrozine, Nitroso- 

PSAP, Xylidyl Blue-I, and 5-Br-PAPS methods. 

In the field of clinical and biomedical research, metallo quantification is important for understanding the role of metal ions 

in biological processes, such as iron in hemoglobin or copper in enzymes. Monitoring metal ion concentrations is also vital 

for diagnosing and managing various diseases, including iron-deficiency anemia and Wilson's disease. 

Metastasis Assays 

Metastasis is a critical process in cancer biology, where cancer cells spread from the primary tumor to other parts of the 

body, often leading to advanced disease and poor patient outcomes. The IN SITE Metastasis Hydrogel Kit and Metastasis 

Surface Coating Kit are instrumental tools in studying and understanding this complex phenomenon. By recreating a 

71


microenvironment that mimics the conditions for metastatic cancer cell growth, researchers can gain insights into the 

mechanisms and factors influencing cancer spread. This information is invaluable in developing targeted therapies, assessing 

treatment responses, and ultimately improving the prognosis and quality of life for cancer patients. 

Additionally, these kits have applications in drug development, personalized medicine, and the investigation of novel 

therapeutic approaches to combat metastatic disease. 

Additional Assays 

Apoptosis Detection and Related Assays 

• Annexin V-APC/7-AAD Apoptosis Detection Kit: This assay allows for the identification of apoptotic cells by labeling 

them with Annexin V and 7-AAD, distinguishing between early and late apoptotic cells. 

• Caspase-3 Activity Colorimetric Assay Kit: It measures caspase-3 activity, a key enzyme in the apoptotic pathway. 

Researchers use this kit to assess apoptosis and cell death in various cell types. 

• TUNEL Apoptosis Assay: The TUNEL assay detects DNA fragmentation, a hallmark of apoptosis, by labeling the ends 

of DNA fragments. It's widely used in cancer research, drug development, and understanding programmed cell death. 

Cell Senescence and Related Assays 

• Fluorescence Senescence β-Galactosidase Activity Assay Kit: This assay detects senescent cells, which have stopped 

dividing and may play a role in aging and various diseases. 

• Chemical Senescence β-Galactosidase Activity Assay Kit: Similar to the fluorescence-based assay, this kit is used to 

assess cellular senescence and its impact on cell populations. 

72


Nucleic Acid and Telomere Length Assays 

• Absolute and Relative Telomere Length Assay Kits: These kits are vital for measuring telomere length, which is linked 

to aging and various diseases, including cancer and cardiovascular conditions. 

• qRT-PCR Kits: These quantitative reverse transcription-polymerase chain reaction kits are used to analyze gene 

expression and are crucial for studying genes involved in cell cycle control and senescence. 

Metabolic and Redox Assays 

• Biopyrrin ELISA kit, Redox Assay: This assay quantifies biopyrrin, a marker for oxidative stress. It's applied to study 

oxidative damage in various biological systems. 

• Lactate Dehydrogenase (LDH) Assay: LDH is an enzyme released during cell damage. This assay helps assess cellular 

cytotoxicity and damage, often used in cytotoxicity studies and assessing cell health. 

• Creatinine, Urinary, ELISA Kit: Used to measure urinary creatinine levels, this assay aids in evaluating kidney function 

and diagnosing renal diseases. 

Cell Viability and Proliferation Assays 

• MTT and WST-1 Cell Viability & Proliferation Assays: These assays assess cell viability, metabolic activity, and 

proliferation. They are employed in drug screening, cytotoxicity testing, and assessing the effects of various 

compounds on cell health. 

TR-FRET 

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a powerful and versatile molecular assay technique 

used in biological research and drug discovery. TR-FRET allows scientists to study molecular interactions and events within 

cells or biochemical reactions with high sensitivity and precision. It is particularly valuable for measuring protein-protein 

interactions, receptor-ligand binding, and various other biomolecular processes. 

TR-FRET relies on the principles of fluorescence resonance energy transfer (FRET), in which energy is transferred from one 

fluorophore (the donor) to another (the acceptor) when they are in close proximity, typically within 10-100 angstroms. The 

key distinction in TR-FRET is the measurement of the time delay between excitation and emission, which eliminates shortlived 

background fluorescence and significantly enhances signal-to-noise ratios. 

To perform TR-FRET assays, researchers require specific reagents: 

• Donor and Acceptor Fluorophores: These are the fluorescent molecules that emit light when excited by an energy 

source. The donor fluorophore, typically a lanthanide chelate (e.g., Europium or Terbium), is excited by a pulsed 

73


light source, and its emission is transferred to the acceptor fluorophore (e.g., XL665 or APC), resulting in a FRET 

signal. 

• Target Molecules: These are the biological components under investigation, such as proteins, nucleic acids, or 

small molecules, labeled with the donor or acceptor fluorophores. The choice of labeling strategy depends on the 

specific experiment and the accessibility of the target sites. 

• Buffer Solutions: Appropriate buffer solutions are used to maintain physiological conditions and optimize the 

stability of the labeled molecules. 

• Time-Resolved Detection System: This includes a fluorescence plate reader or a dedicated TR-FRET reader 

equipped with the capability to measure the time delay between excitation and emission of the fluorophores. 

TR-FRET has revolutionized the field of molecular biology and drug discovery by enabling the study of complex cellular 

processes in a high-throughput and sensitive manner. Researchers can use TR-FRET to assess biomolecular interactions, 

screen for potential drug candidates, and gain insights into cellular pathways and mechanisms with exceptional accuracy 

and efficiency. 

How to order? 

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Ordering/Requesting 

• You can order from PELOBiotech by phone, email, fax and mail. Please note, if you use our Online- 

Shop you will get an order confirmation with your request via email or fax, it´s not an automated 

order. Please note, we sell only to commercial customers (B2B). We do not sell to private 

consumers (B2C). 

• Online Requests are possible for all countries except Austria, Switzerland and Japan. Please order 

via our great partners. 

• To pay by credit card, please send your credit card information through. Please note that a 2% fee 

will be added to all credit card payments. VISA and Mastercard only. 

• Phone customer services direct on +49 (0) 89 517 286 59 0 

• Email your orders to sales@pelobiotech.com or fax to +49 (0) 89 517 286 59 88 

Orders can be placed with us as follows: 

How to order? 

PELOBIOTECH GmbH 

Am Klopferspitz 19 

82152 Planegg / Germany 

Telephone: +49 (0) 89 517 286 59 0 

Fax: +49 (0) 89 517 286 59 88 

E-mail: sales@pelobiotech.com 

Bartelt Gesellschaft m.b.H. 

Neufeldweg 42 

8010 Graz 

Austria 

Global Call: +43-(0)316 475 328 

Global Fax: +43-(0)316 475 328-55 

Email: office@bartelt.at 

abc biopply AG 

Gewerbestrasse 8 

CH-6330 Cham 

Switzerland 

Global Call: +41-(0)41 747 25 43 

Global Fax: +41-(0)41 743 25 36 

Email: service@biopply.com 

BizCom Japan, Inc. 

5-25-4 Higashiikebukuro, Toshima-ku 

Tokyo 170-0013 

Japan 

Global Call: +81-(0)3 6277 3233 

Global Fax: +81-(0)3 6277-3265 

Email: service@bizcomjapan.co.jp 

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PELOBiotech GmbH 

E-Mail: 

info@pelobiotech.com 


Lothar Steeb, Peter Frost (CEOs) 

For more information 

Phone: +49 (0)89 517 286 59-0 

Fax: +49 (0)89 517 286 59-88 


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