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_____________________________________________________________ Results and Discussion replaced by the capacitance of the DNA monolayer (CDNA). Cd is represented by the following equation: 1 = 1 + 1 (3.6) C d C DNA C ddl Upon DNA immobilization, Cd (Figure 3.16) and subsequently Cddl values (Figure 3.17) were obtained in relation to the applied potential using PDEIS. In this case, the investigated potential range was shifted to -0.4 V to 0.5 V (vs. Ag/AgCl/3 M KCl) to avoid the cleavage of the Au-S bond at higher potentials. The pzc (DNA) was determined to be around 0.1 V (vs. Ag/AgCl/3 M KCl) showing that the pzc shifts to more negative values due to the surface modification with DNA. Unlike in the case of the bare polycrystalline gold electrode, for the DNA-modified electrode a well-defined minimum was observed. A possible explanation is a more homogeneous surface after the modification with the DNA layer. Furthermore, the broad minimum is in agreement with the GC double layer model that assumes peak broadening with decreasing ionic strength 43 . Figure 3.16. Determination of Cd at varying applied potentials at the DNA-modified electrode. Impedance was measured for two ionic strengths: 10 mM PB with 20 mM K2SO4 and 0.1 mM PB with 0.2 mM K2SO4. Cd was calculated at a frequency of 10 Hz. DNA immobilization: 0.5/-0.2 V pulse profile (10 ms pulse duration, vs. Ag/AgCl/3 M KCl), 5 min in 1 µM DNA solution prepared in 10 mM PB with 450 mM K2SO4. Procedure explained in the Section 3.3.1. 3.2 Importance of knowing the surface 48
_____________________________________________________________ Results and Discussion Figure 3.17. Determination of the pzc of the DNA-modified electrode. Cddl values were calculated using the data from Figure 3.16 obtained at a frequency of 10 Hz. 3.2 Importance of knowing the surface 49
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Jambrec Daliborka - 2016 DISSERTATI
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Acknowledgements For me, the PhD st
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“A structure this pretty just had
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5. Experimental Work
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18. Canaria C. A.; So J.; Maloney J
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61. Mikkelsen S. R.: Electrochecmic
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microarray using a biotinylated int
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