abstracts - INCDCSZ Brasov

COMMUNITY RESOURCES FOR HIGH THROUGHPUT GENOME MAPPING
AND DIVERSITY ANALYSES IN 1EBN POTATO SPECIES
Iorizzo M. 1,2 , Aversano R. 2 , Carputo D. 2 , Kilian A. 3 , Wenzl P. 3 , & Bradeen J. M. 1
1
Department of Plant Pathology, University of Minnesota (MN, USA)
2
Department of Soil, Plant, Environmental and Animal Production Sciences, University of Naples
“Federico II” (ITALY)
3
Diversity Arrays Technology, Pty Ltd (ACT, AUSTRALIA)
Efficient access to genetic variability is important for breeding programs. Wild potato species
represent rich genetic resources for potato improvement. The USDA potato Genebank maintains more
than 5,000 accessions of 135 potato species. Included are 20 closely related diploid potato species
classified collectively as “superseries Stellata” that are of particular interest as sources of resistance to
biotic and abiotic stress (Table 1). Among potato species, crossability is predicted by the endosperm
balance number (EBN), with sexually compatible species sharing a common EBN. Most of the
Stellata species are 1EBN, making them sexually incompatible with cultivated potato (4EBN). But
genes from these species can be accessed using bridge crosses, somatic hybridization, and gene
cloning and transformation. To improve access to agriculturally significant genes from Stellata species
we have initiated an effort of comparative structural genomics using the Diversity Array Technology
(DArT) marker platform (Jaccoud et al. 2001). DArT is a high throughput, microarray based
Series a
Table 1. Superseries Stellata
Species EBN Reported Disease Resistance b
I. Morelliformia S. clarum ? Early blight, PVM, Verticillium
II. Bulbocastana S.
bulbocastanum
1
Blackleg, Bacterial wilt, Early blight, Late blight,
PLRV, PVM Ring rot, Verticillium, Wart
S.
cardiophyllum 1 Late blight, Ring rot, Verticillium, Wart
III. Pinnatisecta S. pinnatisectum 1 Black leg, Early blight, Late blight, PLRV, Ring rot
S. jamesii 1 Blackleg, PLRV, PVY, Ring rot, Verticillium
S. tarnii 1 Late blight, Ring rot
S. trifidum 1 Late blight, PLRV, Ring rot, Verticillium
IV. Polyadenia S. polyadenium 1
Blackleg, Late blight, PLRV, PVA, PVM, Ring rot,
Verticillium
V.
Bacterial wilt, Blackleg, Early blight, Ring rot,
Commersoniana S. commersonii 1 Verticillium
VI. Circaeifolia S. circaeifolium 1 Bacterial wilt, PVM, PVS, Rhizoctonia, Wart
VII. Lignicaulia S. lignicaule 1 Fusarium wilt, Rhizoctonia, Verticillium, Wart
VIII. Olmosiana S. olmosense ?
IX. Yungasensa S. chacoense 2 PVA, PVF, PVX, PLRV, Verticillium
ªClassification scheme for superseries Stellata of Hawkes (1990) with modifications after
Rodriguez and Spooner (1997), Lara-Cabrera and Spooner (2004), and Spooner et al. (2004).
b Resistance data from personal experience, published accounts (Austin et al. 1993; Helgeson
et al. 1998; Micheletto et al. 2000; Naess et al. 2000; Song et al. 2003; Vossen et al. 2005; Vossen et al.
2003), and USDA Potato Genebank website.
technology capable of detecting thousands of polymorphic markers dispersed throughout the genome.
Because data generation entails nucleic acid hybridization to a common set of immobilized markers,
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