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BABA respond to pathogen infection <strong>by</strong> rapidly increasing the expression <strong>of</strong> PR genes.<br />

Another feature that sets BABA resistance apart from the other two types is the ability to<br />

not only protect the plant from biotic stress but also from abiotic stress (Jakob et al.,<br />

2005).<br />

One <strong>of</strong> the underlying themes <strong>of</strong> induced disease resistance in the last few years<br />

is the cross-talk that exists between the three types <strong>of</strong> resistance. Although each appears<br />

to have its unique characteristics, genes and molecules that were once thought to be<br />

exclusive to one type <strong>of</strong> resistance appear to also possess a role in one or both <strong>of</strong> the<br />

other types (Pieterse and Van Loon, 2004).<br />

2.4 TGA <strong>factors</strong> in SAR<br />

2.4.1 The as-1 element<br />

TGA <strong>factors</strong> were first identified through their ability to bind to the activating<br />

sequence 1 (as-1) element <strong>of</strong> the Cauliflower Mosaic Virus (CaMV) 35S promoter<br />

(Katagiri et al., 1989). It is from the as-1 element, composed <strong>of</strong> two TGACG motifs<br />

spaced eight nucleotides apart, that TGA <strong>factors</strong> derive their name. Research done<br />

concurrently found that TGA <strong>factors</strong> were also able to bind a similar element found in<br />

the octopine synthase (ocs) promoter from the Agrobacterium tumefaciens tumour-<br />

inducing (Ti) plasmid (Fromm et al., 1989). Consequently some <strong>of</strong> the TGA <strong>factors</strong><br />

were first referred to as Octopine Binding Factors (OBF) (Zhang et al., 1993); however<br />

they will be referred to as TGA <strong>factors</strong> in this thesis.<br />

The as-1 element has been found to be important in the <strong>regulation</strong> <strong>of</strong> glutathione<br />

S-transferase 6 (GST-6) (Strompen et al., 1998) as well as PR-1 (Lebel et al., 1998). It<br />

has also been found to be over-represented in the promoters <strong>of</strong> genes differentially<br />

8

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