regulation of arabidopsis tga transcription factors by cysteine ...

Table 3.1 Media used in this study.
Culture Type a Components b
Bacteria 2YT 1.7% tryptone, 1% yeast extract, 0.5% NaCl
Bacteria SOC 2% tryptone, 0.5% yeast extract, 8.5 mM NaCl, and 2.5 mM
KCl, (10 mM MgCl2 and 200 mM glucose after autoclaving)
Yeast SD 2% glucose, 0.67% yeast nitrogen base without amino acids,
0.15% of the appropriate dropout powder c
a
Media was autoclaved at 20 psi (pounds per square inch) at 121°C for 30 min on the
liquid cycle.
b
For bacteria and yeast solid media, 1.5% and 2 % agar was added respectively.
c
Prepared as per instructions (Kohalmi et al., 1997).
Table 3.2 Antibiotics used in this study.
Antibiotic Stock solutions* Final concentration in
media
Carbenicillin 50 mg/ml in water (filter sterilized) 12.5 mg/L
Chloramphenicol 25 mg/ml in 100% ethanol 25 mg/L
*Stock solutions stored at -20°C.
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