regulation of arabidopsis tga transcription factors by cysteine ...

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LIST OF TABLES CHAPTER 3: MATERIAL AND METHODS Table 3.1 Media used in this study ................................................................................... 24 Table 3.2 Antibiotics used in this study............................................................................ 24 Table 3.3 Oligonucleotides used for PCR amplification and sequencing......................... 31 viii
LIST OF FIGURES CHAPTER 2: LITERATURE REVIEW Figure 2.1 Dendrogram of Arabidopsis TGA factors ........................................................... 11 CHAPTER 4: RESULTS Figure 4.1 Transactivation and NPR1-interaction properties of TGA1 containing mutations within the 30 amino acid region........................................................................... 38 Figure 4.2 Alignment of seven of the Arabidopsis TGA factors.......................................... 39 Figure 4.3 β-galactosidase assay results for TGA1 and TGA4 site-directed mutants used in this study. .......................................................................................................................... 41 Figure 4.4 Quantitative GUS assay of transactivation of TGA1 with mutation of the cysteine residues ................................................................................................................... 44 Figure 4.5 Quantitative GUS assay of transactivation of TGA1 with mutation at multiple cysteine residues ................................................................................................................... 52 Figure 4.6 β-galactosidase assay results for TGA1/TGA2 chimeric and site-directed mutants used in this study ..................................................................................................... 54 Figure 4.7 β-galactosidase assay results for TGA1/TGA2 chimeric and site-directed mutants used in this study ..................................................................................................... 58 CHAPTER 5: DISCUSSION Figure 5.1 A model depicting redox control of TGA1 on binding activity (modified from Després et al., 2003).............................................................................................................. 65 ix
Page 1 and 2: REGULATION OF ARABIDOPSIS TGA TRANS
Page 3 and 4: ACKNOWLEDGEMENTS I would like to th
Page 5 and 6: argument that oxidoreduction of thi
Page 7: 3.2.5.2 Electroporation of electro-
Page 11 and 12: GSSG oxidized glutathione GUS β-gl
Page 13 and 14: CHAPTER 1: INTRODUCTION All plants
Page 15 and 16: 2.1 Induced disease resistance CHAP
Page 17 and 18: PR genes like acd2 (accelerated cel
Page 19 and 20: localization of NPR1, which is medi
Page 21 and 22: expressed under abiotic stress and
Page 23 and 24: TGA2 TGA6 TGA5 TGA1 TGA4 TGA3 TGA7
Page 25 and 26: overexpression of a single member o
Page 27 and 28: closely related tobacco gene TGA2.1
Page 29 and 30: maintaining the reducing environmen
Page 31 and 32: the cell wall, HR, as well as act a
Page 33 and 34: consisted of the N-terminus of TGA2
Page 35 and 36: 3.2.2 Bacterial media Liquid and so
Page 37 and 38: culture volume of cold sterile 10%
Page 39 and 40: 3.3.2 Yeast Plasmids The yeast plas
Page 41 and 42: 20 µl. The ligation mixture was in
Page 43 and 44: Table 3.3 Oligonucleotides used for
Page 45 and 46: oligonucleotides to amplify from th
Page 47 and 48: fluorescence by a Perkin Elmer LS50
Page 49 and 50: CHAPTER 4: RESULTS 4.1 Role of cyst
Page 51 and 52: TGA1 M-----NSTSTHFVPPRRVGIYEPVHQFGM
Page 53 and 54: Description Transactivation Wild ty
Page 55 and 56: of the best characterized targets o
Page 57 and 58: To ensure that GUS activity observe
Page 59 and 60:
The codon for Cys at position 256 o
Page 61 and 62:
construct showed a slight decrease
Page 63 and 64:
not significantly different to that
Page 65 and 66:
4.2 Attempts to alter transactivati
Page 67 and 68:
pBI881 and transformed them into ye
Page 69 and 70:
Description Transactivation Wild ty
Page 71 and 72:
CHAPTER 5: DISCUSSION Following the
Page 73 and 74:
protein, interacted with NPR1 in ye
Page 75 and 76:
TGA1/TGA4. These results indicate t
Page 77 and 78:
Pathogen S S HS HS TGA1 TGACG NPR1
Page 79 and 80:
TGA1 C260S SDMs display a great dec
Page 81 and 82:
protein to interact with NPR1. The
Page 83 and 84:
preferred partner of Cys-260 in dis
Page 85 and 86:
incorporated the corresponding Cys
Page 87 and 88:
CHAPTER 6: REFERENCES Alvarez,M.E.,
Page 89 and 90:
Friedrich,L., Lawton,K., Dietrich,R
Page 91 and 92:
Mahoney,C.W., Pak,J.H., and Huang,K
Page 93 and 94:
Sticher,L., Mauch-Mani,B., and Metr
Page 95:
Zimmerli,L., Jakab,G., Metraux,J.P.
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