MGIT TM Procedure Manual - Foundation for Innovative New ...

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Section I: Principle of Procedures TABLE OF CONTENTS A. Introduction B. Principle of the BACTEC MGIT 960 TB System 1. MGIT medium 2. Principle of detection and susceptibility testing Section II: Procedure for Primary Isolation A. Introduction B. Important Safety Precautions C. Specimen Handling 1. Collection 2. Transportation 3. Storage D. Digestion, Decontamination and Concentration 1. Sputum a. NaOH-NALC procedure b. Other procedures c. Important points 2. Specimens other than sputum (extra-pulmonary) a. Pus and other mucopurulent specimens b. Gastric aspirates c. Bronchial washings d. Laryngeal swabs e. Tissue f. Urine g. Other body fluids E. Smears for Acid-Fast Bacteria (AFB) 1. Smear preparation 2. Staining methods a. Ziehl-Neelsen staining b. Kinyoun’s staining c. Two-step staining d. Fluorochrome acid-fast staining 3. Microscopy 4. Reporting MGIT TM Procedure Manual 3
Page 1: Prepared for the Foundation for Inn
Page 5 and 6: TABLE OF CONTENTS Section III: Drug
Page 7 and 8: PREFACE The purpose of this procedu
Page 9 and 10: Section I: Principle of Procedure A
Page 11 and 12: 2. Principle of detection and drug
Page 13 and 14: Section II: Procedure for primary i
Page 15 and 16: 1. Sputum Section II: Procedure for
Page 17 and 18: Section II: Procedure for Primary I
Page 19 and 20: . Gastric aspirates Section II: Pro
Page 23 and 24: Procedures Section II: Procedure fo
Page 27 and 28: • Make all additions inside a bio
Page 33 and 34: d. Cross-contamination Section II:
Page 35 and 36: J. Limitations of the Procedure Sec
Page 39 and 40: a. Positive and negative controls S
Page 41 and 42: Section III: Drug Susceptibility Te
Page 43 and 44: 4. Procedures a. Reconstitution of
Page 45 and 46: Drug Concentration of drug after re
Page 51 and 52: 5. Results Section III: Drug Suscep
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Section IV: References 1 Isenberg H
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MGIT Procedure Manual Section IV: R
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Section IV: Appendices APPENDIX A S
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2. Supplies for AFB smears (continu
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APPENDIX B Miscellaneous Procedures
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3. AFB stains Appendix B Note: Alre
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. Sodium hydroxide solution Appendi
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APPENDIX C Procedures for Troublesh
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Appendix C • Check if the incubat
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Appendix C � Certain digestion/de
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Appendix C • Was the Growth Suppl
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Appendix C � Periodic sterility t
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APPENDIX D Guidelines for Susceptib
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Appendix D Day 0 - the day a MGIT t
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• Do not add drug to the growth c
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Appendix D • Usually higher conce
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BD has not validated drugs other th
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