MGIT TM Procedure Manual - Foundation for Innovative New ...

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TABLE OF CONTENTS F. Preparation and Inoculation for Culture 1. Reagents a. MGIT medium b. MGIT growth supplement (enrichment) c. MGIT PANTA 2. Procedures a. Reconstituting PANTA b. Inoculation of MGIT medium c. Inoculation of additional media d. Precautions e. Incubation f. Detection of positive growth G. Work-up of Positive Cultures 1. AFB smear from a positive MGIT tube 2. Dealing with contamination a. Bacterial contamination b. Isolation of mycobacteria from contaminated or mixed cultures c. Isolation of mixed mycobacterial culture on Middlebrook Agar Plate d. Cross-contamination 3. Sub-culturing a positive MGIT tube 4. Identification of isolated mycobacteria H. Results / Reporting I. Performance Characteristics J. Limitations of the Procedure K. Quality Control 1. Quality control of AFB smear staining 2. Quality control (QC) testing of MGIT medium a. QC strains b. Preparation of culture suspension c. Preparation of dilutions d. Inoculation/incubation e. Expected results f. Precautions 3. Quality control of laboratory procedures a. Positive and negative controls b. Quality control with laboratory data 4. Record keeping MGIT TM Procedure Manual 4
TABLE OF CONTENTS Section III: Drug Susceptibility Testing A. Primary Drug Susceptibility Testing (SIRE) 1. Introduction 2. Principles of the test 3. Reagents a. Drugs b. SIRE supplement c. Storage 4. Procedures a. Reconstitution of lyophilized drugs b. Addition of a drug to the medium c. Preparation of the inoculum d. Inoculation and incubation 5. Testing at higher drug concentrations 6. Results 7. Reporting 8. Quality control (QC) B. Pyrazinamide (PZA) Susceptibility Testing 1. Introduction 2. Principles of the test 3. Reagents 4. Procedures a. Reconstitution of lyophilized PZA drug b. Preparation of the inoculum c. Inoculation and incubation 5. Results 6. Reporting 7. Quality control C. Secondline Drug Susceptibility Testing Section IV: References MGIT TM Procedure Manual 5
Page 1: Prepared for the Foundation for Inn
Page 6 and 7: Section V: Appendices TABLE OF CONT
Page 8 and 9: MGIT TM Procedure Manual 8
Page 10 and 11: Section I: Principle of Procedures
Page 12 and 13: MGIT TM Procedure Manual 12
Page 14 and 15: Section II: Procedure for Primary I
Page 20 and 21: f. Urine Section II: Procedure for
Page 22 and 23: . Kinyoun’s staining Section II:
Page 24 and 25: 4. Reporting Section II: Procedure
Page 26 and 27: 2. Procedures a. Reconstituting PAN
Page 28 and 29: f. Detection of positive growth Sec
Page 30 and 31: 2. Dealing with contamination Secti
Page 38 and 39: d. Inoculation/incubation Section I
Page 40 and 41: 4. Record keeping Section II: Proce
Page 42 and 43: Section III: Drug Susceptibility Te
Page 46 and 47: 5. Testing at higher drug concentra
Page 50 and 51: c. Inoculation and incubation Secti
Page 52 and 53: C. Secondline Drug Susceptibility T
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MGIT Procedure Manual Section IV: R
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Page 58 and 59:
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MGIT TM Procedure Manual
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2. Supplies for AFB smears 212522 T
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3. Supplies for drug susceptibility
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Procedure for Ordering Cultures App
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c. Auramine O fluorescent (fluoroch
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MGIT TM Procedure Manual B-6
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Appendix C • Do not use reagents
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Appendix C � Is transport time to
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Appendix C � More than 25 minutes
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Appendix C • Were the tubes inocu
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MGIT TM Procedure Manual C-10
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Appendix D � The personnel who wo
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Precautions Appendix D • Use a pu
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h. Interpretation of results Append
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Appendix D • Borderline resistant
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