Book of abstracts version 5

Synthesis of a core-shell Molecular Imprinted Polymer for
the isolation of 10-deaccetylbaccatine lll out of Taxus
10-DAB
Author
Ismael Nouhi
Academy of Technology for Health and Environment
Avans Hogeschool, Breda
Lectoraat Avans
Sonny van Seters
Abstract
In this research, an attempt was made to make a core-shell molecularly-inprinted polymer (MIP) by means of emulsion
polymerisation with the aim of isolating 10-deacetylbaccatine III (10-DAB) from raw Taxus Baccata L. extract. The core
structure was prepared using methyl methacrylate (MMA) as a functional monomer and ethylene glycol dimethacrylate
(EGDMA) as a crosslinking agent. The shell was made by emulsion polymerization with 10 -DAB as template molecule, 2-
vinylpyridine (2-VP) as functional monomer and trimethylolpropane trimethacrylate (TRIM) and EGDMA as cro ss-linking
agents. The synthesized MIP and NIP was characterized by thermogravimetric analysis (TGA), Fourier transform infrared
spectroscopy (FTIR) and Differential scanning calorimetry (DSC). The MIPs and NIPs made were washed with methanol,
the different fractions were measured with a UV spectrophotometer and high -pressure liquid chromatography (HPLC) for
detection of 10-DAB and impurities in the fractions. A conversion of 89% was achieved for the core seed particles with a
weight percentage of 71.1 mg / ml solid particles in polymer solution made. Initial conversions between 20% and 30%
were achieved for making the MIP and NIP. Adjustment and optimization of the recipe resulted in a conversion of 59%
within a reaction time of 2 hours.
Keywords: Molecularly imprinted polymer, NIP, 10-DAB, Paclitaxel, core-shell. emulsion polymerisation.
Table of content
Figure 1: Schematic representation principle of the molecular imprinting technique.
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