Research Report 2003 - Max-Planck-Institut für molekulare Genetik

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Department of Vertebrate Genomics 16 allows automatic analysis of >1000 protein samples/per day and forms the basis for our ongoing proteome projects in human brain and Arabidopsis thaliana (see below) and collaborations. In collaboration with the group of Dr. Ralf Rabus (Max Planck Institute for Marine Microbiology, Bremen) the first proteomic study of the recently sequenced organism Pirellula was performed, in which over 1000 new proteins from this organism was identified (manuscript in preparation). (b) MALDI mass spectrometry – new and improved sample preparation methods The physical process of MALDI-TOF mass spectrometry is inherently fast (
different tissues of A. thaliana, which can serve as a basis for proteomic and biological investigations in this plant. This task was recently accomplished and a manuscript describing the results has been submitted (Giavalisco et al. 2003b). (f) Human brain proteome analysis The NGFN platform technology project “Development of Platform Technologies for Functional Proteome Analysis – Application to Human Brain” is performed in collaboration with several academic and industrial partners. A new calibration routine for MALDI-TOF spectra was developed that improves the mass accuracy (Gobom et al. 2002). In collaboration with the company Protagen AG (Dortmund), and Prof. Klose (Charité), over 1000 brain-specific proteins have been identified (Chamrad et al. 2003), which will serve as the basis for protein-protein interaction studies and the development of a protein chip. (g) Clinical proteomics A recently initiated project within the NGFN is the Proteomverbund, which aims integrate state-of-the-art proteomic technology in clinical research. Within this consortium we have, in collaboration with the research group of Dr. Seegert at the University of Kiel, initiated a study aiming to discern aberrant protein expression/modification in chronic inflammatory bowel disorder. Planned developments / future orientation NGFN-2 - Our group is well-established within the NGFN and we plan to maintain these efforts by applying for funding in the 2nd round of NGFN. Here, we aim to extend the use of our established technology in clinical proteomic studies in collaboration with medical networks. EU-FP6 - Within the project Molecular phenotyping we aim to extend our technology to the proteomic analysis of post-translational modifications and quantitative analysis, to perform differential analysis of patient/control samples for the discovery of disease markers. A Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry laboratory has been established. This technique offers, by its unique available fragmentation modes, the possibility for detailed protein structural characterization. The implementation of this advanced mass spectrometric technique in proteomic research is in progress. Ultra Structure Network (USN) As an extension of work carried out within the protein structure factory, we plan to address the question of the composition and structure of larger protein complexes by a combination of mass spectrometry to identify the components of complexes, and Cryo EM to analyze their structure (Bodo Lange). MPI for Molecular Genetics Research Report 2003 17
Page 1 and 2: Max-Planck-Institut für molekulare
Page 3 and 4: Table of Contents The Max Planck In
Page 5 and 6: MPI for Molecular Genetics Research
Page 7 and 8: The Max Planck Institute for Molecu
Page 9 and 10: Department of Vertebrate Genomics I
Page 11 and 12: To generate high resolution express
Page 13 and 14: Competitive position Since a long t
Page 15 and 16: the Protein Structure Factory. Fina
Page 17: Mass Spectrometry Group Head: Dr. J
Page 21 and 22: Bioinformatics Group Heads: Ralf He
Page 23 and 24: a) Evolutionary studies In a recent
Page 25 and 26: Fuchs T, Malecova B, Linhart C, Sha
Page 27 and 28: Mouse, Medaka & MHC Group Head: Dr.
Page 29 and 30: General information Publications 19
Page 31 and 32: Jochen Wittbrodt, EMBL, Heidelberg
Page 35 and 36: THETA - Two Hybrid Transfected-cell
Page 37 and 38: Academical co-operations Andreas Ra
Page 39 and 40: The investigation of signaling path
Page 41 and 42: In vitro Ligand Screening Group Hea
Page 45 and 46: Neurodegenerative Disorders Group H
Page 47 and 48: Automation Group Graduate students:
Page 49 and 50: In addition, novel methods for the
Page 51 and 52: Sittler A, Walter S, Wedemeyer N, H
Page 53 and 54: Prof. Dr. Joachim Klose, Institut f
Page 55 and 56: eral years. This ambiguity is partl
Page 57 and 58: Clark MD, Panopoulou GD, Cahill DJ,
Page 59 and 60: integrations were passaged to the g
Page 63 and 64: Protein Group Scientists: Sabine Ba
Page 65 and 66: Kersten B, Bürkle L, Kuhn EJ, Giav
Page 67 and 68: Cardiovascular Genetics Group Head:
Page 69 and 70:
protein interaction partners and at
Page 71 and 72:
General information Publications 19
Page 73 and 74:
Chromosome 21 Group Head: Marie-Lau
Page 75 and 76:
Page 77 and 78:
Department of Human Molecular Genet
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Network (NGFN). Recently, DNA from
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Finally, several of these groups ar
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Neurochemistry Group & Mouse Lab Gr
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studies. Furthermore, these mice wi
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Noonan syndrome and related disorde
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Chromosome Rearrangements & Disease
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General information Selected Public
Page 93 and 94:
DNA Microarrays Scientists: Dr. Fik
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General information Publications He
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that in addition to reduced prolife
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Christoph Redies, Prof. Dr., Instit
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Since my move to the MPIMG in 11/20
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Biochemistry of Inherited Brain Dis
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Schweiger S, Chaoui R, Tennstedt C,
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Until 2002, 13 genes have been impl
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General information Selected public
Page 111 and 112:
Department of Computational Molecul
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EU: The European Molecular Biology
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EST tissue distribution Besides the
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Protein Families & Evolution Group
Page 119 and 120:
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We make use of the well-known HMMer
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Theses Benjamin Georgi: A graph-bas
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wide analysis of tyrosine as well a
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Further projects include multiple t
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Co-operations Identification and fu
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upstream of the translational start
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Department of Developmental Genetic
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2000; see figure 1). So far, we hav
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partners are. We also want to find
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Emeritus Group General Molecular Ge
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Sethmann S, Ceglowski P, Willert J,
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Research Group Development & Diseas
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MPI for Molecular Genetics Research
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Goals An important future goal is t
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Independent Junior Research Groups
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over, daf-9 is tightly regulated by
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European C.elegans Meeting, Blanken
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and at the ribosomal DNA locus. One
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lencer, has so far remained unchara
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Molecular control of skeletal devel
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BMPs, into a common control network
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this end I plan to analyze mouse mu
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Ribosome Group The Ribosome group c
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General information Selected Public
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group during the same period. In ad
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11) Agmon I, Auerbach T, Baram D, B
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Reconstitution & function The ribos
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Wendrich TM, Blaha G, Wilson DN, Ma
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Miscellaneous Research Groups There
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vealed that the extraordinary stabi
Page 181 and 182:
34. Thorsted PB, Macartney DP, Akht
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esis and DNA packaging of this phag
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Other launched projects concern con
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BMBF 01GR0105, Plattform 1.1: NGFN
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DNA synthesis, lipid biosynthesis,
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Administration & Research Support H
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Technical Management and Workshops
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tion based on the simplified GOOD a
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Library Librarians: Dipl.-Fachinf.
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How to get to the Institute Max Pla
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