PELONews April/May 2019
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<strong>PELONews</strong><br />
We have talked: Optimised Cancer<br />
Treatments and EV research with 3D Models<br />
To an expert- talk about “Optimised Cancer Treatments and EV research with 3D Models”<br />
invited PELOBIotech GmbH and its Swiss partner abc biopply at the IZB Sky-Lounge end of<br />
March <strong>2019</strong>. Around 20 scientists from Bavarian biotech companies and institutes such as<br />
TUM, LMU, Helmholtz Centre in Munich and Fraunhofer in Regensburg discussed with highlevel<br />
cancer research experts Dr. med. Andreas Thomsen and Dr. Irina Nazarenko from Universitätsklinikum<br />
Freiburg about new models for tissue-specific interaction of cells and how<br />
they optimize their treatments.<br />
First Dr. Marco Leu, Co-Owner abc biopply, gave a short overview of a novel and innovative 3D microwell array in<br />
the analysis of adhesion independent micro-organoids, named 3D CoSeedis. “I am glad abc biopply was able to<br />
collaborate with Dr. Andreas Thomsen and to further develop this unique and novel technology. We are proud<br />
to have recently released the first standardizes systems it to the scientific world” says Dr. Leu. 3DCoSeedis allows<br />
the support of the growth of feeder cells in the formation of 3D cell constructs that are not in direct contact with<br />
the test cells themselves.<br />
„It’s totally cool!“<br />
Both key speakers are already working with the new system 3D CoSeedis, as in fact Dr.<br />
Thomsen invented the system himself. His lecture “Creating Unique Microenvironments<br />
in 3D Models” showed a broad range of applications and inspired the researchers on their<br />
projects on tissue-specific interaction of cells. A lot of wows he earned showing his results<br />
in comparison with standard 2d methods and the new 3D model. “It makes it so much easier”,<br />
the Freiburg radio-oncologist says. His survey “Do you enjoy colony formation assays?” performed among<br />
colleagues made the audience laugh. They knew almost everybody dislike it and now are given a possibility to<br />
work much more efficient.<br />
A lot of interest had the lecture by Dr. Irina Nazarenko: Studying Intercellular Communication by the mean of<br />
Extracellular Vesicles in 3D Models. She talked about the impact environment on production, cargo, and function<br />
of extracellular vesicles (EVs) in several cancer models. EVs mediate intercellular communication between tumor<br />
and tumor stroma, tumor and immune system, playing a pivotal role in metastases, immunosuppression, angiogenesis<br />
and other systemic effects in progressing tumor. In her experience 3DCoSeedis TM is applicable for EV isolation,<br />
analytic and functional characterization. Using the 3D microwell array more EVs/cell can be isolated in a<br />
considerably more cost- and efforts- efficient manner than 2D culture. “My co-workers and me love working with<br />
it”, Dr. Nazarenko says, as the cells are growing in more physiological environment and producing more EVs. 3D<br />
environments are likely to trigger release of smaller EVs with increased amounts of certain miRNAs and decreased<br />
amounts of their target proteins. She also emphasized that functional impact of 3D environment on EVs<br />
differs between cell lines and should be individually analyzed.<br />
„We are already switching!“<br />
Dr. Nazarenko is more than convinced by the 3D CoSeedis system. Asked whether she would<br />
switch if she had the opportunity: “Definitely, it´s genius, totally cool. So, where it makes<br />
sense, we are already switching, step by step”, she outlines. One of the participants confirms<br />
her point of view: “We are more and more searching for 3D models, also in EV research. So<br />
this system sounds very promising to us.”<br />
Peek into presentation Dr. Irina Narzarenko and download paper here.<br />
Download presentation Dr. med. Andreas Thomsen, and download paper here:<br />
Peek into presentation Dr. Marco Leu