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May/June <strong>2019</strong><br />

Evaluation of Nitrogen Stabilizers to Improve Corn<br />

Yield and Plant Nitrogen Status<br />

Weed Identification: A Crucial Component of Weed<br />

Management<br />

Evaluation of Grafted Tomato Plants for<br />

California Fresh Market Producton Systems<br />

Walnut Husk Fly Management<br />

MAY/JUNE <strong>2019</strong><br />

VINEYARD<br />

REVIEW<br />

pages 36-50<br />

See<br />

Page 15<br />

September 26th-27th<br />

Visalia Convention Center<br />

303 E. Acequia Ave. Visalia, CA 93291<br />

PUBLICATION<br />

Volume 4 : Issue 3<br />

May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

1


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2 Progressive Crop Consultant May/June <strong>2019</strong><br />

© <strong>2019</strong>, Trécé Inc., Adair, OK USA • TRECE, PHEROCON and CIDETRAK are registered trademarks of Trécé, Inc., Adair, OK USA • TRE-1383, 2/19<br />

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Your Edge – And Ours – Is Knowledge.


4<br />

IN THIS ISSUE<br />

Evaluation of Nitrogen<br />

Stabilizers to Improve<br />

Corn Yield and Plant<br />

Nitrogen Status<br />

PUBLISHER: Jason Scott<br />

Email: jason@jcsmarketinginc.com<br />

EDITOR: Kathy Coatney<br />

ASSOCIATE EDITOR: Cecilia Parsons<br />

Email: article@jcsmarketinginc.com<br />

PRODUCTION: design@jcsmarketinginc.com<br />

Phone: 559.352.4456<br />

Fax: 559.472.3113<br />

Web: www.progressivecrop.com<br />

12<br />

18<br />

26<br />

30<br />

36<br />

42<br />

48<br />

Weed Identification: A<br />

Crucial Component of<br />

Weed Management<br />

Evaluation of Grafted<br />

Tomato Plants for<br />

California Fresh Market<br />

Producton Systems<br />

Walnut Husk Fly<br />

Management<br />

ACP Control with Systemic<br />

Insecticides<br />

VINEYARD REVIEW<br />

Grapevine Heat Stress and<br />

Sunburn Management<br />

Grapevine Trunk Diseases:<br />

Current Management<br />

Strategies<br />

Pierce’s Disease and Glassywinged<br />

Sharpshooter: Still<br />

a Threat to California Viticulture<br />

4<br />

18<br />

VINEYARD<br />

REVIEW<br />

36<br />

CONTRIBUTING WRITERS &<br />

INDUSTRY SUPPORT<br />

Brenna Aegerter<br />

UCCE Farm Advisor for<br />

San Joaquin County<br />

Whitney Brim-Deforest<br />

UCCE Rice Advisor<br />

Akif Eskalen<br />

Department of Plant<br />

Pathology - UC Davis<br />

Michelle Leinfelder-<br />

Miles<br />

Delta Farm Advisor, UC<br />

Cooperative Extension,<br />

San Joaquin County<br />

Kevin Day<br />

County Director and<br />

UCCE Pomology Farm<br />

Advisor, Tulare/Kings County<br />

Steven T. Koike,<br />

Director, TriCal Diagnostics<br />

Emily J. Symmes<br />

Sacramento Valley<br />

Area IPM Advisor<br />

University of California<br />

Cooperative Extension<br />

and Statewide IPM<br />

Program<br />

José Ramón Úrbez-<br />

Torres<br />

Agriculture and Agri-<br />

Food Canada<br />

Stephen Vasquez<br />

Technical Viticulturist,<br />

Sun-Maid Growers<br />

George Zhuang UCCE<br />

Fresno County<br />

UC COOPERATIVE EXTENSION<br />

ADVISORY BOARD<br />

Emily J. Symmes<br />

UCCE IPM Advisor,<br />

Sacramento Valley<br />

Kris Tollerup<br />

UCCE Integrated Pest<br />

Management Advisor,<br />

Parlier, CA<br />

The articles, research, industry updates, company profiles, and<br />

advertisements in this publication are the professional opinions<br />

of writers and advertisers. Progressive Crop Consultant does<br />

not assume any responsibility for the opinions given in the<br />

publication.<br />

May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

3


Evaluation of Nitrogen Stabilizers to Improve<br />

Corn Yield and Plant Nitrogen Status<br />

By MICHELLE LEINFELDER-MILES<br />

| Delta Farm Advisor, UC Cooperative Extension, San Joaquin County<br />

Introduction<br />

Nitrogen (N) is part of a balanced,<br />

natural cycle in the<br />

environment among the<br />

atmosphere, soil, plants, animals, and<br />

water. Nitrogen is the most important<br />

element needed by crops, and we often<br />

add nitrogen fertilizer to optimize crop<br />

productivity. Nitrogen use in agricultural<br />

systems must be reported for regulatory<br />

compliance under the Irrigated<br />

Lands Regulatory Program and the<br />

Dairy Order to help ensure that a greater<br />

fraction of the applied N is recovered<br />

in the harvested crop and not lost to the<br />

environment. Nitrogen management<br />

gives consideration to the four R’s:<br />

• Right source: selecting a fertilizer<br />

source that matches with crop need and<br />

minimizes losses.<br />

• Right rate: applying the right amount<br />

based on crop need and nutrient<br />

availability through other sources.<br />

• Right time: applying the nutrient<br />

when the crop can use it.<br />

• Right place: fertilizer placement that<br />

optimizes the crop’s ability to use it.<br />

The four R’s address management<br />

considerations (e.g. fertilizer program,<br />

irrigation), but site characteristics<br />

(e.g. soil, cropping system, weather<br />

conditions) also influence N recovery in<br />

the crop. Also important to improving<br />

crop N recovery is understanding<br />

barriers to adopting best management<br />

practices, such as costs or risks to crop<br />

quality or yield.<br />

While the four R’s articulate four<br />

principles for nitrogen management,<br />

the N cycle in cropping systems<br />

is complicated. Nitrogen can be<br />

introduced and lost by various paths.<br />

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Continued on Page 6<br />

4 Progressive Crop Consultant May/June <strong>2019</strong>


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May/June <strong>2019</strong><br />

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5


Continued from Page 4<br />

organic matter amendments—such as<br />

crop residues, compost, or manure.<br />

Fertilizers provide N in plant-available<br />

forms—ammonium (NH 4<br />

+) and nitrate<br />

(NO 3 -). Organic matter amendments<br />

must be mineralized before the<br />

N is available for plant uptake.<br />

Mineralization is a process that involves<br />

soil biology converting organic N to<br />

NH 4<br />

+. The timeline of this conversion<br />

will depend on the properties of<br />

the amendment, environmental<br />

conditions—such as soil temperature<br />

and moisture, and the activity and<br />

abundance of soil microbes.<br />

In the soil, NH 4<br />

+ has different fates. It<br />

can be immobilized by microorganisms,<br />

taken up by plants, fixed to soil particles<br />

due to its positive charge, volatilized to<br />

ammonia gas (i.e. lost from the system),<br />

or converted to NO 3 -—a process<br />

known as nitrification. Nitrification is<br />

a two-step process. The first step is the<br />

conversion of NH 4<br />

+ to nitrite (NO 2 -)<br />

by Nitrosomonas bacteria. The second<br />

step is the conversion of NO 2 - to nitrate<br />

(NO 3 -) by Nitrobacter bacteria. These<br />

two steps generally occur in close<br />

succession to prevent the accumulation<br />

of NO 2 - in the soil. Conditions that<br />

affect nitrification include soil aeration,<br />

moisture, temperature, pH, clay and<br />

cation content, NH 4<br />

+ concentration,<br />

among others. Just as NH 4<br />

+ has different<br />

THE FOUR R’s<br />

Right source: selecting<br />

a fertilizer source that<br />

matches with crop need and<br />

minimizes losses<br />

Right rate: applying the<br />

right amount based on<br />

crop need and nutrient<br />

availability through other<br />

sources,<br />

Right time: applying the<br />

nutrient when the crop can<br />

use it,<br />

Right place: fertilizer<br />

placement that optimizes<br />

the crop’s ability to use it.<br />

fates in the soil, so too does NO 3 -. Plants<br />

preferentially take up NO 3 -, but if NO 3 -<br />

is present when plants are not in need<br />

of it, then NO 3 - may be immobilized<br />

by microorganisms, volatilized to<br />

nitrogen gas (i.e. lost from the system),<br />

or leached out of the root zone (i.e. lost<br />

from the system).<br />

Technologies have been developed<br />

to mitigate N losses from the system.<br />

These technologies are collectively<br />

known as enhanced efficiency<br />

fertilizers (EFF) and include additives,<br />

physical barriers, and chemical<br />

formulations that stop, slow down,<br />

or decrease fertilizer losses. Nitrogen<br />

stabilizers, slow-release fertilizers, and<br />

polymerized fertilizers are examples of<br />

EEF. Nitrogen stabilizers are fertilizer<br />

additives intended to improve crop<br />

N use efficiency and reduce N losses<br />

to the environment by interrupting<br />

the microbial processes that change<br />

N to its plant-available forms. We<br />

developed a trial to evaluate two N<br />

stabilizer products with the objective<br />

of determining whether the treatments<br />

improved corn silage yield or plant<br />

N status compared to fertilizer alone.<br />

We did not attempt to measure N<br />

losses from the system (e.g. leaching,<br />

denitrification), as these are very<br />

challenging to quantify.<br />

The products in our trial were<br />

Vindicate (Corteva Agriscience) and<br />

Agrotain Plus (Koch Agronomic<br />

Services). Vindicate delays the<br />

nitrification process by inhibiting<br />

the Nitrosomonas bacteria that<br />

converts NH 4<br />

+ to NO 2 -. Vindicate has<br />

bactericidal activity, and the active<br />

ingredient is nitrapyrin. Agrotain Plus<br />

has two modes of action—reducing<br />

ammonia volatilization and delaying<br />

nitrification. Ammonia volatilization<br />

is the conversion of NH 4<br />

+ in the<br />

soil to ammonia gas (NH 3 ) in the<br />

atmosphere, and it is reduced by<br />

inhibiting the urease enzyme. Ammonia<br />

volatilization is most problematic<br />

when the N source is urea-based and<br />

not incorporated or watered into the<br />

soil. The active ingredients of Agrotain<br />

Plus are Dicyandiamide (DCD), which<br />

delays nitrification, and N-(n-butyl)-<br />

thiophosphoric triamide (NBPT),<br />

which reduces volatilization. DCD has<br />

bacteriostatic activity, which means it<br />

slows the metabolism of Nitrosomonas.<br />

We hypothesized that N stabilizers<br />

would improve yield and N uptake over<br />

the fertilizer-only treatment, providing<br />

growers with a tool for nutrient<br />

stewardship.<br />

Methods<br />

The trial took place in San Joaquin<br />

County on a DeVries sandy loam soil.<br />

The field had a winter wheat crop that<br />

was cut for forage in the late spring. Dry<br />

manure was applied to the field between<br />

wheat harvest and corn planting, which<br />

occurred on May 24, 2018. The variety<br />

was Golden Acres 7718. At-planting<br />

fertilizer provided approximately 12 lb<br />

N per acre (4-10-10). Sidedress fertilizer<br />

application occurred on June 21st and<br />

provided approximately 105 lbs N per<br />

acre (UAN 32). Four treatments were<br />

Continued on Page 8<br />

6 Progressive Crop Consultant May/June <strong>2019</strong>


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7


Continued from Page 6<br />

Figure 1. Nitrogen stabilizers applied at sidedress fertilizer application.<br />

applied at sidedress, when plants were<br />

at V3-4 stage of development (Figure.<br />

1). The N stabilizers were applied at the<br />

label rates, and the treatments were: 1)<br />

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TM<br />

Vindicate at 35 fluid ounces per acre, 2)<br />

Agrotain Plus at 3 pounds per acre, 3)<br />

combination of Vindicate and Agrotain<br />

Plus at aforementioned rates, and 4)<br />

fertilizer-only, no<br />

stabilizer product<br />

(“untreated”). Plots<br />

were 35 feet across<br />

(i.e. fourteen 30-inch<br />

rows), in order to<br />

adapt to equipment<br />

of different widths,<br />

by 900 feet long.<br />

Treatments were<br />

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We evaluated soil<br />

N status, plant<br />

N status, and<br />

silage yield. Prior<br />

to planting,<br />

20 soil cores<br />

were randomly<br />

collected from<br />

across the trial<br />

and aggregated by<br />

foot-increments,<br />

down to two feet.<br />

Mid-season leaf<br />

and soil samples<br />

were collected<br />

when the corn was in the R1 stage (i.e.<br />

silking). Soil was collected from 10<br />

in-row locations in each treatment, and<br />

aggregated by foot-increments, down to<br />

two feet. Leaves were sampled from ten<br />

plants in each treatment, sampling the<br />

leaf one-below and opposite the earleaf.<br />

Harvest occurred on September 20th.<br />

All fourteen rows were harvested for<br />

weight, and samples were collected at<br />

the silage pit for aboveground biomass<br />

N analysis. The samples were dried<br />

at 60⁰C for 48 hours for calculating<br />

dry matter (DM). Post-harvest, 10<br />

in-row soil cores were collected to<br />

one-foot depth and aggregated for<br />

each treatment. Laboratory analyses<br />

were conducted by Ward Laboratories<br />

(Kearney, NE; https://www.wardlab.<br />

com/). We used Analysis of Variance<br />

to detect differences in treatments and<br />

Tukey’s range test for means separation<br />

(JMP statistical software). Treatments<br />

were considered statistically different if<br />

the P value was less than 0.05.<br />

Continued on Page 10<br />

“ Technologies have been<br />

developed to mitigate N<br />

losses from the system. These<br />

technologies are collectively<br />

known as enhanced efficiency<br />

fertilizers...<br />


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May/June <strong>2019</strong><br />

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9


Table 1. Plant N, yield, dry matter (DM), and N removed results for the 2018 N stabilizer efficacy trial. There were no<br />

significant differences among treatments.<br />

TABLE 1<br />

Treatment<br />

Midseason (R1)<br />

Leaf Total N<br />

(%)<br />

Aboveground<br />

Biomass Total<br />

N<br />

(%)<br />

Yield at<br />

30% DM<br />

(tons/acre)<br />

DM<br />

(%)<br />

Total N Removed at<br />

Harvest<br />

(lbs N/acre)<br />

Vindicate 2.97 1.12 40.4 0.37 272<br />

Agrotain Plus 2.97 1.11 37.7 0.34 250<br />

Vindicate and<br />

Agrotain Plus<br />

2.71 1.16 38.7 0.34 269<br />

Untreated 2.87 1.09 38.3 0.35 251<br />

Average 2.88 1.12 38.8 0.35 261<br />

CV (%) 4 2 3 3 5<br />

P value 0.32 0.18 0.48 0.20 0.39<br />

Continued from Page 8<br />

Results and Discussion<br />

There were no statistically significant<br />

differences among treatments for plant<br />

tissue N, yield, dry matter, or total N<br />

removed at harvest (Table 1). Midseason<br />

leaf N averaged 2.88 percent<br />

across treatments, and aboveground<br />

biomass N at harvest averaged 1.12<br />

percent. At mid-season, leaf N from 2.7<br />

to 3.5 percent indicates that the plants<br />

had sufficient N to carry the crop to<br />

harvest, and at harvest, whole plant<br />

N from 1.0 to 1.2 percent indicates<br />

that the N fertilization program was<br />

adequate for maximizing yield [1].<br />

Calculated to 30 percent dry matter,<br />

average yield across treatments was<br />

38.8 tons/acre, and dry matter was<br />

35 percent. There was a trend for the<br />

two treatments with Vindicate to have<br />

a higher N removed than the two<br />

treatments without it, but the difference<br />

was not statistically significant. The low<br />

coefficient of variation (CV), which is a<br />

measure of variability in relation to the<br />

mean, indicates low variability among<br />

replicates for all of these parameters.<br />

The pre-plant (post-dry manure<br />

application) soil nitrogen status was<br />

17 parts per million (ppm) NO 3 -N<br />

and 4 ppm NH 4 -N for the 0-12 inch<br />

depth, and 7 ppm NO 3 -N and 2 ppm<br />

NH 4 -N for the 12-24 inch depth. When<br />

soil NO 3 -N is below 25 ppm in the<br />

top foot of soil, it is recommended to<br />

apply N fertilizer in order to prevent<br />

yield reductions [2]. There were no<br />

differences among treatments in soil<br />

N status at the mid-season sampling,<br />

but there were differences at the postharvest<br />

sampling (Table 2. See page,<br />

11). Mid-season soil NO 3 -N averaged<br />

32 ppm across treatments in the top<br />

foot of soil, and 10 ppm in the second<br />

foot of soil, which is an adequate<br />

concentration to carry the crop through<br />

to harvest. Soil NH 4 -N averaged 4 ppm<br />

and 2 ppm across treatments for the top<br />

10 Progressive Crop Consultant May/June <strong>2019</strong>


Table 2. Soil N status (as NO3-N and NH4-N) at mid-season and post-harvest samplings for the 2018 N stabilizer<br />

efficacy trial.<br />

TABLE 2<br />

Treatment<br />

Mid-season<br />

NO 3-N (ppm)<br />

0-12 inches<br />

Mid-season<br />

NO 3-N (ppm)<br />

12-24 inches<br />

Mid-season<br />

NH 4-N (ppm)<br />

0-12 inches<br />

Mid-season<br />

NH 4-N (ppm)<br />

12-24 inches<br />

Post-harvest<br />

NO 3-N (ppm)<br />

0-12 inches<br />

Post-harvest<br />

NH 4-N (ppm)<br />

0-12 inches<br />

Vindicate 33 10 4 2 38 b 2<br />

Agrotain Plus 32 10 4 2 44 ab 2<br />

Vindicate and<br />

Agrotain Plus<br />

32 9 3 2 57 a 2<br />

Untreated 31 12 4 2 43 ab 2<br />

Average 32 10 4 2 46 2<br />

CV (%) 25 31 20 36 7 19<br />

P value 0.99 0.75 0.58 0.97 0.04 0.89<br />

foot and second foot, respectively. The<br />

CV was high for mid-season soil data,<br />

which indicates high variability among<br />

replicates. Post-harvest soil NH 4 -N<br />

averaged 2 ppm across treatments in<br />

the top foot of soil, but soil NO 3 -N<br />

was higher than at any other time<br />

during the season, averaging 46 ppm<br />

across treatments. These results may<br />

indicate that<br />

the dry manure<br />

mineralized<br />

later in the<br />

season, after the<br />

peak demand<br />

of the corn.<br />

Post-harvest<br />

soil NO 3 -N<br />

above 20 ppm is<br />

considered high and indicates that this<br />

crop was not deficient in N [2]. The low<br />

CV for NO 3 -N indicates low variability<br />

among replicates. The significant<br />

differences among treatments are not<br />

well-understood, particularly as the<br />

control (fertilizer-only) treatment had<br />

soil NO 3 -N that was not different from<br />

any of the treatments. Interestingly,<br />

Vindicate had the lowest post-harvest<br />

soil NO 3 -N and a trend toward higher<br />

N removed (though not statistically<br />

higher), which may indicate that<br />

product use made N available at a time<br />

that optimized N uptake.<br />

Summary<br />

N is part of a balanced, natural cycle<br />

“<br />

N is part of a balanced,<br />

natural cycle in the<br />

environment and is the most<br />

important nutrient in cropping<br />

systems.<br />

in the environment and is the most<br />

important nutrient in cropping<br />

systems. Giving consideration to N<br />

management will help ensure that a<br />

greater fraction of the applied N is<br />

recovered in the harvested crop and<br />

not lost to the environment, and keeps<br />

growers in regulatory compliance.<br />

Enhanced Efficiency Fertilizers, such<br />

as N stabilizers,<br />

have been shown<br />

to improve<br />

crop yield in<br />

regions like the<br />

Midwest and<br />

the Northeast,<br />

and may help<br />

to mitigate N<br />

losses from the<br />

environment. In our trial, we evaluated<br />

the efficacy of N stabilizer products for<br />

improvements in corn silage yield or<br />

plant N status compared to fertilizer<br />

alone. Under the management and<br />

environmental conditions of this trial,<br />

we found no differences in yield or plant<br />

N status; however, plant and soil tests<br />

indicated that N was never limiting in<br />

the trial. If N was lost from the system,<br />

the loss was not large enough to result<br />

in N limitation in the control. Future<br />

study should test these products using<br />

different N sources and N rates (e.g.<br />

grower rate and grower rate minus 50<br />

lbs N/acre). It may be possible to reduce<br />

the fertilizer N rate without sacrificing<br />

yield.<br />

“<br />

References<br />

1. Nutrient Management for Field Corn<br />

Silage and Grain in the Inland Pacific<br />

Northwest. https://www.cals.uidaho.edu/<br />

edcomm/pdf/PNW/PNW0615.pdf.<br />

2. Nutrient Management Guide – Silage<br />

Corn (Western Oregon). https://catalog.<br />

extension.oregonstate.edu/em8978.<br />

Comments about this article? We want<br />

to hear from you. Feel free to email us at<br />

article@jcsmarketinginc.com<br />

May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

11


By WHITNEY BRIM-DEFOREST | UCCE Rice Advisor, Yuba/Sutter Counties<br />

Weed identification is the<br />

foundation for weed control.<br />

For both cultural controls<br />

(tillage, weed-whacking, etc.), and<br />

herbicides, misidentification can lead<br />

to wasted time, money, and resources.<br />

But even for experienced weed scientists<br />

and botanists, weed identification<br />

can be difficult. Traditional keys, for<br />

example, primarily rely on our ability<br />

to distinguish between plants at flowering,<br />

and often require a fair amount<br />

of knowledge of botanic terms, and<br />

possibly even a microscope. Aside from<br />

the difficulty of using the keys, identification<br />

at flowering is usually too late for<br />

weed control, particularly for the use of<br />

many herbicides.<br />

There are many tools available to use for<br />

weed identification, ranging from books<br />

to cards, to online databases, and even<br />

computer programs and smartphone<br />

apps. The resources found below are<br />

just a few of the plethora of weed<br />

identification resources, highlighting<br />

some of the most relevant for California,<br />

and many that are free or low-cost.<br />

Print<br />

Printed materials may be a bit difficult<br />

to carry into the field, obviously, but<br />

they can be a good resource, especially<br />

for learning more about the biology and<br />

ecology of the weed species once it is<br />

identified.<br />

1) Weeds of California and Other<br />

Western States (DiTomaso<br />

and Healy, 2007)<br />

This 2-volume set is<br />

available through the<br />

UCANR website (as well<br />

as many other websites),<br />

and contains many,<br />

many weeds found in<br />

California, as well as<br />

those that may be likely<br />

to move into California<br />

from surrounding states.<br />

It includes over 700<br />

weed species, in over<br />

60 families. It also has<br />

tables to help distinguish<br />

between commonlyconfused<br />

weeds.<br />

2) Weed Identification<br />

Cards (DiTomaso, 2013)<br />

This set of cards is<br />

adapted from the Weeds<br />

of California and Other<br />

Western States book listed<br />

above, and contains the 48 most<br />

widely distributed weed species<br />

in California. It is available from the<br />

UCANR website. The weeds are divided<br />

into the following 8 plant groups, for<br />

easy searching:<br />

1. Volumes 1-2 of Weeds of California and<br />

Other Western States (DiTomaso and<br />

Healy, 2007).<br />

12 Progressive Crop Consultant May/June <strong>2019</strong>


• Broadleaf annuals, erect<br />

• Broadleaf annuals, low growing<br />

• Broadleaf annuals, scrambling<br />

• Broadleaf perennials, not viney<br />

• Broadleaf perennials, viney<br />

• Grass annuals<br />

• Grass perennials<br />

• Sedges<br />

The cards are small and can be held in the hand while in the field.<br />

They are laminated, so even if they get moist, they will not be<br />

ruined.<br />

Computer Based<br />

There are a couple of resources available (one USB-loaded program<br />

and one web-based application) if you need some help identifying<br />

weeds, especially during early growth stages. While the USB can<br />

only be used on a computer, the web-based application can also<br />

be used on a smart-phone (in the field), although it does require<br />

connectivity to the web to be able to do so.<br />

Continued on Page 14<br />

2. Weed Identification Cards<br />

(DiTomaso, 2013).<br />

May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

13


Continued from Page 13<br />

1) Weed ID USB (DiTomaso, 2014).<br />

The Weed ID USB contains identification information for 722 broadleaf species<br />

and 200 weedy grasses. Using the program, it is possible to identify weeds even<br />

at the seedling stage, using key characteristics visible to the naked eye, instead of<br />

requiring a microscope or hand lens. For example, key traits such as stem crosssection,<br />

leaf shape, hairs on the leaves, etc. It also allows the user to select the<br />

family, or genus, if known. After making selections, the program will, through<br />

process of elimination, show a list (with photos and descriptions) of all the weed<br />

species fitting those characteristics. Once down to a few species, the user can<br />

visually compare the photos of their specimen to the photos in the database.<br />

The USB is available through the California Invasive Plant Council Website (www.<br />

cal-ipc.org), or by contacting Dr. DiTomaso directly (jmditomaso@ucdavis.edu).<br />

14 Progressive Crop Consultant May/June <strong>2019</strong><br />

2) Online Weed Identification Tool (University of Wisconsin-Madison):<br />

The University of Wisconsin-Madison hosts an online weed identification tool<br />

that is very similar to the USB drive (above). It is freely available at https://weedid.<br />

wisc.edu/weedid.php. Several states are available, so be sure to select California as<br />

the search location. There are far fewer species in this database in comparison to<br />

the USB drive, but the identification method is similar. The user has to select plant<br />

characteristics, and through a process of elimination, the possible weed species<br />

with those characteristics will remain. The user can then use the photos to identify<br />

their specimen.<br />

Continued on Page 16


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Continued from Page 14<br />

Mobile Apps<br />

Although there are several smartphone<br />

applications available for both Androids<br />

and IPhones, testing of several yielded<br />

only one with enough species to make it<br />

worthwhile to use in the field.<br />

1) Pl@ntNet (www.plantnet.org)<br />

The Pl@ntNet app (available for both<br />

Android and IPhone) was created by a<br />

consortium of universities and public<br />

research institutions. It contains plant<br />

species from all over the world, so<br />

the user has to be careful to select the<br />

correct continent (North America). It<br />

does not cover only weeds, however,<br />

which is important to note. It also<br />

contains native and naturalized species.<br />

The app works by matching key photo<br />

characteristics with identified photos<br />

already in the database. The user<br />

takes a photo, then specifies which<br />

characteristic to focus on (leaf, fruit,<br />

bark, flower, habit, or other), then the<br />

app matches the photo with potential<br />

specimens in the database. The user<br />

then selects the species that most<br />

closely matches, and submits it to the<br />

app, where it is reviewed (seemingly<br />

by experts). Upon testing it, it was able<br />

to accurately identify several grasses,<br />

broadleaves, and shrubs.<br />

In-Person Assistance<br />

When in doubt, ask another person<br />

to assist in identification. Fellow pest<br />

control advisors, growers, and other<br />

colleagues are great resources. However,<br />

if it appears to be a new or unknown<br />

weed species in a particular cropping or<br />

natural system, there are other resources<br />

available to help as well.<br />

can assist in identification. Visit the herbarium website at https://herbarium.<br />

ucdavis.edu/services.html for collection and sample delivery protocols. The<br />

herbarium can identify up to five samples per person per year at no charge, and<br />

after that, an hourly rate for identification applies.<br />

There are many more tools available for weed identification beyond the ones listed<br />

above, including many helpful keys and online resources. In order to identify a<br />

weed, it may be necessary to utilize many tools and second opinions, particularly if<br />

it is a less well-known species, or if it is new to a cropping or natural system. While<br />

identification can be time-consuming, especially when we are anxious to get rid<br />

of a weed, ensuring proper identification before deciding on a plan for control can<br />

save a lot of time, energy, and money over the long run.<br />

Comments about this article? We want to hear from you. Feel free to email us at<br />

article@jcsmarketinginc.com<br />

1) University of California Cooperative<br />

Extension advisors and specialists<br />

County-based advisors and campusbased<br />

specialists can be helpful in<br />

providing weed identification. Local<br />

offices are located in almost every<br />

county in California, and advisors there<br />

can tap into larger networks of weed<br />

scientists for help with identification,<br />

if they are unable to identify the weed<br />

themselves.<br />

2) Weed identification at the UC Davis<br />

Herbarium<br />

For really tricky cases, the UC Davis<br />

Herbarium has botanists on staff that<br />

16 Progressive Crop Consultant May/June <strong>2019</strong>


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17


Evaluation of Grafted Tomato Plants<br />

for California Fresh Market Production Systems<br />

By BRENNA AEGERTER | UCCE Farm Advisor for San Joaquin County<br />

All photos courtesy of Brenna Aegerter<br />

Why Graft?<br />

Grafting involves joining a<br />

fruit-producing shoot (called<br />

the 'scion') of a desirable cultivar<br />

onto the disease-resistant rootstock<br />

of another cultivar. For example,<br />

let’s say you normally grow the cultivar<br />

'QualiT-47' for fruit production, but<br />

that cultivar is susceptible to a soilborne<br />

disease problem in your fields, then you<br />

could graft the top part of a 'QualiT-47'<br />

seedling onto the root-portion of a<br />

more disease-resistant cultivar. In the<br />

case of tomato rootstocks, the majority<br />

of the cultivars are interspecific hybrids<br />

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between cultivated tomato (Solanum<br />

lycopersicum) and wild tomato species<br />

(most commonly Solanum habrochaites,<br />

or less often S. peruvianum or S.<br />

cheesmaniae). Solanum habrochaites is<br />

known from other published research<br />

to be tolerant of salinity, drought, cold<br />

temperatures, and resistant to many<br />

soilborne diseases and many of these<br />

benefits have been demonstrated to be<br />

conferred to the grafted plant when an<br />

interspecific hybrid rootstock is used.<br />

Most of us are familiar with grafting as<br />

a standard practice for California fruit<br />

and nut trees and grapevines, but it<br />

has experienced only<br />

limited commercial<br />

The coating of Anti-Stress<br />

becomes effective when the<br />

product has dried on the plant.<br />

The drying time of Anti-Stress is<br />

the same as water in the same<br />

weather conditions.<br />

adoption among<br />

annual crops in<br />

California thus far.<br />

Grafted tomato<br />

transplants are<br />

commonly utilized<br />

in the commercial<br />

greenhouse industry,<br />

where tomatoes are<br />

produced under<br />

protected culture<br />

and are generally<br />

grown over a much<br />

longer production<br />

cycle, often a<br />

10-month period.<br />

There are greenhouse<br />

producers in Southern<br />

California, but it is<br />

more common in<br />

British Columbia,<br />

Ontario, Mexico<br />

and other US states<br />

(Arizona and<br />

others). In many<br />

countries in Latin<br />

America, Europe<br />

and Asia, grafted<br />

plants represent a<br />

large percentage of<br />

the tomato industry.<br />

For example, in Spain, 50 to 70 million<br />

grafted plants are grown annually for<br />

greenhouse production systems. There<br />

has also been some adoption of grafting<br />

by high-tunnel tomato growers in the<br />

eastern United States. In Southern<br />

California, the nursery Plug Connection<br />

is marketing grafted tomatoes to home<br />

gardeners, dubbing them as a “Mighty<br />

‘Mato”. This allows a home gardener to<br />

grow an heirloom tomato variety, which<br />

often has little or no disease resistance,<br />

without worrying about rotation<br />

or other soilborne disease control<br />

measures.<br />

Our goal was to evaluate the potential<br />

for grafting standard tomato cultivars<br />

onto rootstock cultivars that possess<br />

resistance to soilborne diseases and<br />

nematodes. Our primary objective<br />

was to evaluate the yield performance<br />

of grafted plants in replicated trials<br />

in commercial fresh market (“mature<br />

green”) production fields in the<br />

northern San Joaquin Valley. Our team<br />

consisted of myself, Scott Stoddard with<br />

University of California Cooperative<br />

Extension (UCCE) in Merced County,<br />

and Michael Grieneisen and Minghua<br />

Zhang in the Department of Land, Air<br />

and Water Resources at the University<br />

of California, Davis. This project<br />

produced the first publicly-available<br />

research results on grafted tomatoes for<br />

California production systems.<br />

How is it Done?<br />

For each tray of grafted tomatoes to<br />

be produced, two trays of seed are<br />

sown; one tray of the rootstock seed<br />

and another tray of the scion seed. At<br />

approximately one month after sowing,<br />

the young seedlings are grafted. Both<br />

seedlings are cut at the hypocotyl, and<br />

the scion shoot is spliced onto the<br />

Continued on Page 20<br />

18 Progressive Crop Consultant May/June <strong>2019</strong>


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19


Production of splice-grafted tomato plants for our field trials.<br />

Step 1<br />

Step 2<br />

Step 3<br />

Continued from Page 18<br />

rootstock stump. The method we used<br />

is a commonly used splice-graft with<br />

small, soft, silicone clips to hold the<br />

scion and rootstock together during<br />

healing. Grafted transplants cost more<br />

than non-grafted transplants due to<br />

increased seed costs and the labor<br />

required to do the grafting. Thus far,<br />

grafted tomato plants are only available<br />

from a few sources in California, and<br />

we won’t know what the cost for grafted<br />

tomato transplants will be until they are<br />

being produced in larger volumes here.<br />

The use of fully- or semi-automated<br />

grafting robots is emerging as a way<br />

to reduce labor costs and improve the<br />

survival rate of grafted plants. This<br />

of course requires significant capital<br />

investment. About 20 seed companies<br />

offer tomato rootstock seeds (see list<br />

at http://www.vegetablegrafting.org/<br />

tomato-rootstock-table/). However, for<br />

a nursery facility or grower considering<br />

doing their own grafting, building a<br />

Step 1. Scion and rootstock<br />

shoots, with stems of about the<br />

same diameter, are cut at 45°<br />

angles.<br />

Step 2. A soft silicone plastic clip<br />

is placed on the rootstock stump.<br />

The scion cutting is placed into<br />

the clip, aligning the angled cuts.<br />

Grafting success rates are highest<br />

when the diameters of the two<br />

stems to be joined are similar.<br />

Step 3. The plants are then<br />

transferred to a healing chamber<br />

with high humidity, low light<br />

and moderate temperatures for<br />

about a week to allow the graft<br />

union to heal. The plants are then<br />

transferred back to finish growing<br />

in the greenhouse.<br />

healing chamber may be a hurdle. There<br />

is research underway by others to look<br />

at conducting the one-week healing<br />

period inside the greenhouse. For more<br />

information on the logistics of grafting<br />

on a commercial scale, please see the<br />

Vegetable Grafting Manual, the link<br />

for which is provided at the end of this<br />

article under “More information”.<br />

Field Trials in the Northern San<br />

Joaquin Valley<br />

The trials were conducted in commercial<br />

production fields at six locations<br />

over three years from 2016 to 2018;<br />

three locations in San Joaquin County<br />

and another three locations in Merced<br />

County. The treatments included all<br />

combinations of the scions and rootstocks<br />

listed in Table 2 (See page 22).<br />

The plots were laid out in a randomized<br />

complete-block design with four<br />

replicate blocks, each block measuring<br />

approximately 80 by 40 feet. The<br />

cooperating growers managed the<br />

experimental plots similarly to the<br />

rest of their field with respect to pest<br />

control, fertilization, irrigation, and<br />

other management practices. Plants<br />

were mechanically transplanted into<br />

prepared beds at a 4- to 5-inch depth<br />

per normal practice; the graft union<br />

ended up well below the soil surface.<br />

In staked or trellised production<br />

systems in other regions, the graft<br />

union is typically kept above ground to<br />

realize the full benefit of the rootstock<br />

pathogen resistance. With graft union<br />

buried below the soil surface, soilborne<br />

pathogens may attack the scion crown<br />

tissues or adventitious roots arising<br />

from the scion. Due to the lack of<br />

significant pathogen pressure in our<br />

fields, we believe this was not an issue<br />

for these trials.<br />

In our trials, grafted plants were more<br />

vigorous and had better foliage cover of<br />

fruit at harvest than non-grafted plants<br />

of the same cultivar. We also measured<br />

NDVI (Normalized Difference<br />

Vegetation Index, a measure of the<br />

“greenness” or how much of the bed is<br />

covered with actively photosynthesizing<br />

foliage) and it was also slightly higher<br />

in grafted plots. Averaged across all<br />

six trials, marketable yield increased<br />

only 12 percent when grafting with<br />

‘Maxifort’ or ‘DRO138TX’ as the<br />

rootstock, although the results were<br />

better in some individual trials. At the<br />

San Joaquin County sites, yields of<br />

non-grafted vines were similar to the<br />

statewide average yield and grafting<br />

increased yield significantly (25 to 40<br />

percent depending on the year). Some<br />

scion-rootstock combinations were as<br />

much as 68 percent higher than the<br />

non-grafted plants of the same scion<br />

(e.g. ‘QualiT-27’ on ‘Maxifort’ at the San<br />

Joaquin site in 2018). At the Merced<br />

sites, yields of non-grafted vines were<br />

well above-average and grafting was<br />

much less beneficial. Many published<br />

field trials indicate that the yield<br />

advantages of grafted plants are greatest<br />

under sub-optimal growing conditions.<br />

Field sites with heavy soilborne disease<br />

pressure, or abiotic stresses may be the<br />

best candidates to see improvements<br />

with grafting.<br />

Fruit Size and Quality<br />

Many published studies have found<br />

that grafted plants produce a higher<br />

Continued on Page 22<br />

20 Progressive Crop Consultant May/June <strong>2019</strong>


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Pre- and post-Planting Nematode Management in<br />

Panel Discussion—Got Questions on Walnut and Almond<br />

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Varieties—Get Them Answered Here<br />

Caroline Eberlein, Postdoc, UC Riverside CE Credits: 30 Minutes; Other<br />

Cliff Beumel, Sierra Gold Nursery, Steve Rothenberg, Dave Wilson Nursery, and Tom Burchell<br />

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Research Updates on Almond Insect Pests: Navel Orangeworm,<br />

Walnut Board<br />

Leaffooted Bug, and Ants<br />

Jennifer Williams, Assistant Marketing Director, Walnut Board<br />

Kris Tollerup, UCCE Area-wide IPM Advisor CE Credits: 30 Minutes; Other<br />

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Research Updates on Walnut Insect Pests: Navel Orangeworm,<br />

Walnut Husk Fly, and Pacific Flatheaded Borer<br />

Jhalendra Rijal, UCCE Area IPM Advisor, Merced, San Joaquin, and Stanislaus Counties<br />

CE Credits: 30 Minutes; Other<br />

Spray Applications and Preharvest Intervals<br />

Marline Azevedo, Deputy Ag Commissioner, Stanislaus Ag Department CE Credits: 30 Minutes; L & R<br />

An Effective IPM Approach to Mite Control<br />

David Havilland, UCCE Farm Advisor, Kern County CE Credits: 30 Minutes; Other<br />

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NOW—Benefits of Mating Disruption for Almonds and Walnuts<br />

Brad Higbee, Field Research and Development Manager for Trécé Inc. CE Credits: 30 Minutes Other<br />

Adjourn<br />

Are You Ready for FSMAs Produce Safety Rule Inspections?<br />

Priscilla Rodriguez, Director of Food Safety for WAPA<br />

Nutrition Management—Preharvest Preparation for<br />

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Kris Tollerup, Kearney Agricultural Research<br />

Cooperative Extension Advisor, IPM<br />

Devin Clarke, Crop Manager, Tree Nuts, Yara North America<br />

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The Latest on Canker Diseases and<br />

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Supplemental Pollination<br />

Themis Mohammad J Michailides Yaghmour Ph.D., Area Plant Orchard Pathologist Systems UC<br />

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NOW-Benefits of Mating Disruption<br />

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May/June <strong>2019</strong> www.progressivecrop.com 21


Continued from Page 20<br />

percentage of fruit in larger size classes<br />

than those produced by the nongrafted<br />

scion varieties. Averaged over<br />

all our trials, the differences in fruit<br />

size distribution between grafted and<br />

non-grafted were fairly small. In some<br />

trials, however, plants on vigorous<br />

rootstocks did have larger fruit. Some<br />

published studies provide measures of<br />

fruit quality, such as dissolved sugars,<br />

pH, total dissolved solids, vitamin C,<br />

lycopene, or even “taste-test” data.<br />

Those studies indicate that the quality<br />

of fruit from grafted plants seems to be<br />

slightly inferior to fruit from the nongrafted<br />

plants, though still commercially<br />

acceptable. Our field trials focused on<br />

yields, and we did not measure any<br />

fruit quality data. However, we did<br />

not notice any fruit defect problems in<br />

grafted vines. Also, in 2018 we did cut<br />

open both red and mature green fruit at<br />

harvest to make sure that there were no<br />

problems inside the fruit.<br />

Variability From Trial to Trial or<br />

Field to Field<br />

A study in Florida with determinant<br />

type cultivars has shown yield<br />

increases of 25 to 42 percent using<br />

certain rootstocks, but year-to-year<br />

variability also increased as compared<br />

to non-grafted plants. This variation<br />

underscores the importance of<br />

considering variable outcomes to<br />

determine the feasibility of grafted<br />

tomatoes here. Some fields will likely<br />

benefit more from grafting than others,<br />

and this may not always be predictable<br />

in advance.<br />

Economics<br />

Costs of field establishment are<br />

increased significantly with grafting.<br />

Material costs for transplanting (seed<br />

plus nursery costs) alone might be<br />

$2,000 per acre or higher or more<br />

than with conventional transplants.<br />

However, we don’t yet really know what<br />

the costs might be if this were adopted<br />

commercially in California, so our plant<br />

costs are based on small volume sales<br />

prices. If we assume a cost of $0.40 per<br />

grafted plant, then a yield increase of<br />

19 percent at a market price of $6.55<br />

per 25-pound box would pay for the<br />

increased plant cost.<br />

On-going and Future Work<br />

Scion Cultivars<br />

(Trial Years)<br />

‘Bobcat’ (2016) ‘DRO137TX’ (2016-18)<br />

‘Dixie Red’ (2016) ‘Maxifort’ (2016-18)<br />

*‘Galilea’ (2016) ‘Arnold’ (2018)<br />

‘HM 1794’ (2016-18) ‘Guardior’ (2018)<br />

‘Quali T 27’ (2017-18) ‘Estamino’ (2018)<br />

‘Quali T 47’ (2017-18)<br />

‘Quali T 99’ (2017-18)<br />

Other research projects looking<br />

at grafting tomatoes are being<br />

conducted in California. A United<br />

States Department of Agriculture<br />

(USDA)-funded project with<br />

processing tomatoes is underway with<br />

collaboration of Gene Miyao, UCCE<br />

Yolo, Solano and Sacramento counties,<br />

Zheng Wang, UCCE Stanislaus County<br />

and myself, in addition to proprietary<br />

research being conducted by the<br />

industry. Rootstocks for heirloom<br />

tomato production are being evaluated<br />

by Margaret Lloyd, small farms advisor<br />

with UCCE in Yolo, Solano and<br />

Sacramento counties.<br />

Acknowledgements<br />

The California Department of Pesticide<br />

Regulation provided partial funding<br />

for this project but does not necessarily<br />

agree with any opinions expressed, nor<br />

endorse any commercial product or<br />

trade name mentioned. In addition,<br />

this project was supported by the<br />

Specialty Crop Block Grant Program<br />

at the U.S. Department of Agriculture<br />

through Grant 14-SCBGP-CA-0006.<br />

The contents of this report are solely<br />

the responsibility of the authors and<br />

do not necessarily represent the official<br />

views of the USDA. We also thank<br />

our grower-cooperators (Live Oak<br />

Farms and Pacific Triple E), Growers<br />

Transplanting Inc. for producing grafted<br />

Rootstock Cultivars<br />

(Trial Years)<br />

Non-grafted Control<br />

Table 2. Scion and rootstock cultivars used in our field trials.<br />

*Note: Galilea is a roma/saladette type, while the other seven cultivars are all round types; all<br />

but Dixie Red were developed for the Western U.S. mature green production system.<br />

plants, and the following companies<br />

that supplied the seeds: Monsanto/De<br />

Ruiter Seeds, Gowan Seed Company,<br />

Harris Moran Seed Company, and<br />

Syngenta Vegetable Seeds.<br />

For More Information<br />

Additional information on our field<br />

trials:<br />

https://ucanr.edu/sites/veg_crop_sjc/<br />

Grafted_tomatoes/<br />

Detailed information on how to<br />

undertake vegetable grafting is available<br />

at: http://www.vegetablegrafting.org/<br />

resources/grafting-manual/<br />

List of tomato rootstocks including<br />

disease resistances and where to order<br />

seed: http://www.vegetablegrafting.org/<br />

resources/rootstock-tables/solanaceousrootstock-table/<br />

Comments about this article? We want<br />

to hear from you. Feel free to email us at<br />

article@jcsmarketinginc.com<br />

22 Progressive Crop Consultant May/June <strong>2019</strong>


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25


WALNUT HUSK FLY<br />

Management<br />

By EMILY J. SYMMES| Sacramento Valley Area IPM Advisor<br />

University of California Cooperative Extension and Statewide IPM Program<br />

Walnut husk fly poses particular<br />

challenges for developing<br />

a truly integrated pest<br />

management (IPM) program due to<br />

the nature of its life cycle (one generation<br />

per year with a long emergence<br />

period) and lack of natural<br />

enemies. As a result, best practices for<br />

management rely heavily on monitoring<br />

and insecticide treatments. Precise<br />

timing based on monitoring method<br />

and rotation of chemistries to minimize<br />

resistance risk are keys to successful<br />

long-term control of this pest.<br />

Susceptible Varieties<br />

Walnut husk fly (WHF) damage earlier<br />

in the season causes shriveled and<br />

darkened kernels, increased mold<br />

growth, and lower yields. Later season<br />

infestations result in little kernel<br />

damage, but may stain the shells<br />

and make husk removal difficult. All<br />

commercial English walnut cultivars<br />

are susceptible to WHF infestation,<br />

although they differ in their relative<br />

degrees of susceptibility and thus<br />

damage potential. In general, Hartley,<br />

Tulare, Franquette, Payne, and Serr<br />

are considered more susceptible, with<br />

Howard, Ashley, Chico, and Chandler<br />

exhibiting less susceptibility (in<br />

order as listed). However, even less<br />

susceptible varieties can be damaged<br />

by high populations of WHF. Black<br />

walnut is also a preferred host,<br />

therefore proximity to black walnut can<br />

significantly increase WHF pressure in<br />

commercial English walnut orchards.<br />

The varietal differences in susceptibility<br />

have been correlated to fruit<br />

characteristics including husk color,<br />

husk hardness, fruit size, trichome<br />

density, and plant volatile profiles,<br />

in addition to temporal factors (i.e.,<br />

more severe earlier season damage<br />

may be more evident in earlier-leafing<br />

cultivars). Current research led by<br />

Dr. Steven Seybold (United States<br />

Department of Agriculture (USDA)<br />

Chemical Ecology Entomologist)<br />

is characterizing the plant volatile<br />

profiles associated with differences in<br />

varietal susceptibility, which may lead<br />

to improvements in monitoring and<br />

control products, as well as inform<br />

plant breeding approaches for genetic<br />

resistance or tolerance to WHF<br />

infestation.<br />

WHF Life Cycle<br />

The life cycle and basic biology of<br />

walnut husk fly is fairly well understood<br />

(Figure 1. See page 27) There is a<br />

single generation per year, with adult<br />

emergence historically beginning in<br />

early to mid-June and lasting through<br />

September in the Central Valley. In<br />

coastal areas, and recently some inland<br />

valley locations, emergence can be<br />

detected earlier, in mid- to late-May.<br />

Peak emergence is generally observed<br />

July through mid-August in most<br />

locations. Females must mate and<br />

develop eggs prior to the initiation of<br />

oviposition into the walnut husk, a<br />

period which averages approximately<br />

two weeks after emergence. Once<br />

eggs are laid, maggots emerge within<br />

approximately four to seven days, and<br />

feed on the husk for a typical period of<br />

three to five weeks. After this period,<br />

mature maggots drop to the ground and<br />

pupate in the soil. Most adults emerge<br />

the following year, but a portion of the<br />

population may remain in the soil as<br />

pupae for two or more years before<br />

emerging as adults.<br />

Extended Emergence Period<br />

The extended emergence period of<br />

the single generation of WHF, and<br />

significant differences in the timing of<br />

initial emergence, peak emergence, and<br />

end of the flight based on location, year,<br />

and other factors, have been the subject<br />

of much research. As opposed to some<br />

other key pests (e.g., codling moth),<br />

there is not yet a validated phenology<br />

or degree-day model available for<br />

growers and pest control advisors<br />

(PCA) to readily adopt to predict key<br />

WHF development and adult activity<br />

timings. Two recent publications out of<br />

University of California (UC) Berkeley<br />

(Emery and Mills <strong>2019</strong>a, <strong>2019</strong>b)<br />

investigated the effects of temperature<br />

and other environmental parameters<br />

on walnut husk fly development and<br />

timing. One study evaluated 18 years<br />

of historical trap catch data from 49<br />

walnut orchards spanning the Central<br />

Valley to determine which factors<br />

most influence emergence timing and<br />

thermal requirements for development<br />

(degree days to emergence), with<br />

the goal of developing a phenology<br />

model that can be used to predict<br />

initial and peak emergence. Some<br />

of the factors evaluated included<br />

latitude, walnut cultivar, orchard age,<br />

winter precipitation, winter chill, and<br />

26 Progressive Crop Consultant May/June <strong>2019</strong>


Figure 1. Life cycle of walnut husk fly.<br />

Photo courtesy of University of California Statewide Integrated Pest Management Program<br />

degree-day accumulation. While<br />

this model requires refinement for<br />

adoption by orchard practitioners<br />

(growers and PCAs), it represents a<br />

great step forward in improving our<br />

understanding of WHF developmental<br />

requirements to aid in our IPM<br />

program development.<br />

Biological Control Agents<br />

Biological control agents for walnut<br />

husk fly in California walnuts are<br />

virtually non-existent. The pest in<br />

general appears to have few natural<br />

enemies. Some reports from the<br />

state of Washington indicate that a<br />

predatory mite and anthocorid bug<br />

species have been observed feeding<br />

on WHF eggs, and some spiders and<br />

ants may feed on larvae and adults. In<br />

addition, chickens and other birds are<br />

said to be among the natural enemies<br />

of WHF. However, any naturallyoccurring<br />

WHF biological control<br />

agents that may be found in walnut<br />

orchards are not known to provide<br />

any significant level of population<br />

reduction. Other mortality factors,<br />

particularly those that may impact<br />

the overwintering pupal stage in the<br />

soil (e.g., intentionally augmenting<br />

soil moisture, various cultivation<br />

practices, effects or augmentation<br />

of insect-parasitic nematodes or<br />

other microorganism populations,<br />

soil insecticide applications) have<br />

been explored to some degree with<br />

no specific recommendations or<br />

guidelines emerging as a result.<br />

WHF Management Guidelines<br />

In spite of some of these challenges<br />

for WHF management, guidelines<br />

regarding treatment timing and<br />

options are available, and when<br />

employed properly tend to provide<br />

adequate control of WHF in many<br />

situations (albeit with more insecticide<br />

intervention than may be desirable<br />

or sustainable in the long-term).<br />

Because WHF activity and population<br />

abundance can vary significantly<br />

from orchard to orchard (even those<br />

in very close proximity), site-specific<br />

monitoring is necessary to get the<br />

most effective results from insecticide<br />

applications.<br />

Monitoring<br />

Monitoring should begin earlier than<br />

the June 15 historical guideline (no<br />

later than June 1 in the Central Valley<br />

is the more recent recommendation).<br />

Some reports of late May catches in<br />

2016 further support the “earlier-isbetter”<br />

practice—there is little harm<br />

in counting zeroes for a few weeks.<br />

Yellow sticky card traps baited with<br />

ammonium carbonate lures should be<br />

hung high in the canopy (minimum<br />

2 per 10 acres) in dense foliage on the<br />

north side of trees and checked two<br />

to three times per week. Each orchard<br />

should be monitored individually for<br />

WHF activity to best determine if<br />

and when to treat. A summary article<br />

regarding the efficacy of available traps/<br />

lures for WHF monitoring was published<br />

in 2014 (http://www.sacvalleyorchards.<br />

com/walnuts/insects-miteswalnuts/<br />

walnut-husk-fly-trap-and-low-volumespray-study/).<br />

Treatment Timing<br />

Treatment timing can be based on one of<br />

three monitoring methods (the first two<br />

have typically been most effective).<br />

1. Detection of eggs in trapped females.<br />

This is a simple process that requires<br />

slightly more time than counting overall<br />

trap catches and can increase the efficacy<br />

of treatments by timing applications to<br />

specifically target female oviposition<br />

activity. Females can be distinguished<br />

from males by the shape of the abdomen<br />

(pointier in females) and color of the<br />

front leg (female leg is entirely yellow,<br />

male leg is black close to the body,<br />

(Photo 1. See page 28). After females are<br />

identified, gently squishing the female<br />

abdomen will squeeze out eggs if they<br />

are present (Photo 2. See page 28).<br />

Continued on Page 28<br />

May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

27


Continued from Page 27<br />

Eggs resemble small grains of rice.<br />

Previous guidelines indicated that<br />

the treatment window is one week<br />

after egg detection. However, recent<br />

modifications suggest that treatments<br />

should be considered as soon as the<br />

first female with eggs are found because<br />

in practice there is often a lag time in<br />

getting the treatments out, and trap<br />

checks (even two to three checks per<br />

week) may not be frequent enough to<br />

represent initial egg development in the<br />

female population. Therefore, planning<br />

to treat as soon as possible after eggs<br />

are detected may be the best option to<br />

minimize infestation and damage. [Note<br />

that this is the preferred method for<br />

timing treatments unless using GF-120®<br />

alone; see below].<br />

2. Overall trap catches. For low to<br />

moderate populations, consider<br />

treatment when a sharp increase<br />

occurs in trap counts. In high pressure<br />

orchards or if using GF-120® alone,<br />

treatment should be considered when<br />

any flies are detected rather than<br />

waiting for a sharp increase in catches.<br />

3. Stings on nuts. This is the least<br />

preferred method, as damage has<br />

already occurred. However, examining<br />

nuts for stings (Photo 3) can<br />

provide indication of efficacy of your<br />

management program when using one<br />

of the first two methods. If using this<br />

method to time treatments, consider<br />

treating when the first sting is observed<br />

using full cover neonicotinoid materials<br />

that have some ovicidal activity mixed<br />

with an adulticide.<br />

Continued monitoring throughout<br />

the season is crucial. Short-residual<br />

insecticides plus bait will generally<br />

kill WHF for seven to ten days. Target<br />

subsequent applications at two- to fourweek<br />

intervals based on the efficacy of<br />

the previous spray and trap catches.<br />

Clean traps the day after application<br />

and check three to four days later. If<br />

the number of flies drops to near zero,<br />

the spray was highly effective and a<br />

longer treatment interval may be used.<br />

If post-treatment catches increase or<br />

eggs are detected in trapped females,<br />

and the residual period of the previous<br />

treatment has elapsed, additional<br />

treatments may be required if harvest is<br />

more than three weeks away.<br />

There are several materials effective<br />

against WHF, both for conventional and<br />

organic orchards. All materials aside<br />

from GF-120® (which contains its own<br />

bait) should be applied with a bait (e.g.,<br />

Nu-Lure®, molasses, etc.). However,<br />

very high population orchards with<br />

extensive previous damage may require<br />

full coverage sprays (no bait needed) to<br />

achieve adequate suppression. Keep in<br />

mind that rotation of chemistries (based<br />

on the Insecticide Resistance Action<br />

Committee (IRAC) mode of action<br />

classification) is critical to minimize<br />

resistance development for pests that<br />

are treated multiple times each season.<br />

Proper aphid management can also<br />

help limit movement of WHF within<br />

and between orchards by reducing<br />

honeydew accumulation (a food source<br />

for adult WHF).<br />

The UC IPM Pest Management<br />

Guidelines (ipm.ucanr.edu/PMG/<br />

r881301211.html) lists insecticides,<br />

baits, and rates for WHF. A summary<br />

of efficacy data for selected materials<br />

(updated September 2016) are<br />

summarized at (www.sacvalleyorchards.<br />

com/walnuts/insects-mites-walnuts/<br />

walnut-husk-fly-biology-monitoringand-spray-timing/).<br />

Referenced Articles<br />

Emery, S. A. and N. J. Mills. <strong>2019</strong>a.<br />

Effects of temperature and other<br />

environmental factors on the postdiapause<br />

development of walnut husk<br />

fly, Rhagoletis completa (Diptera:<br />

Tephritidae). Physiological Entomology<br />

44: 33-42.<br />

Emery, S. A. and N. J. Mills. <strong>2019</strong>b.<br />

Sources of variation in the adult flight of<br />

walnut husk fly (Diptera: Tephritidae): a<br />

phenology model for California walnut<br />

orchards. Environmental Entomology<br />

48: 234-244.<br />

Comments about this article? We want<br />

to hear from you. Feel free to email us at<br />

article@jcsmarketinginc.com<br />

Photo 1. Male (left) and female<br />

(right) walnut husk fly adults.<br />

Photo 2. Female walnut husk fly with<br />

eggs.<br />

Photo 3. Walnut husk fly sting.<br />

All photos courtesy of University of California<br />

Statewide Integrated Pest Management Program<br />

28 Progressive Crop Consultant May/June <strong>2019</strong>


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29


ACP Control<br />

with Systemic Insecticides<br />

By CECILIA PARSONS | Associate Editor<br />

All photos courtesy of Citrus Disease and Pest Prevention Program.<br />

Last summer’s long hot spell may<br />

have contributed to the low<br />

trap counts of Asian Citrus Psyllid<br />

(ACP) in the Central Valley, but<br />

researchers remain adamant that<br />

keeping the numbers low is the best<br />

defense the state’s citrus belt has to<br />

keep out Huanglongbing (HLB).<br />

Meanwhile, detection of HLB infected<br />

trees in residential areas of the southern<br />

California counties of Orange, Los<br />

Angeles and San Bernardino, continues<br />

to expand.<br />

At the Kern County Spring Citrus<br />

meeting, Dr. Beth Grafton-Cardwell,<br />

director of the University of California<br />

(UC) Lindcove Research and Extension,<br />

said the threat to commercial citrus is<br />

real.<br />

Best Techniques for Reducing<br />

Spread of ACP<br />

Newly hatched ACP nymphs feeding<br />

on an infected tree quickly pick up the<br />

bacterium that causes the disease, and<br />

move on to infect other citrus trees.<br />

Removal of infected trees can help slow<br />

the spread, but detecting an HLBinfected<br />

tree can take time. Grafton-<br />

Cardwell said trees may initially only<br />

be infected on one quadrant and can be<br />

missed in a survey. It may take a year<br />

or two before the entire tree is infected,<br />

diagnosed and removed.<br />

Coordinated spray treatments by<br />

growers when warranted by trap<br />

finds, treating with insecticides that<br />

have an extended residual and being<br />

vigilant about cultural practices are the<br />

important steps in keeping the state’s<br />

citrus industry viable in the face of<br />

HLB.<br />

Movement of stem and leaf material,<br />

whether by harvest crews, hedging and<br />

topping equipment or on spray rigs can<br />

help prevent ACP from hitchhiking to<br />

new territory.<br />

Although ACP finds in the San<br />

Joaquin Valley have been spotty,<br />

Grafton-Cardwell said the coordinated<br />

treatments are a tremendous tool.<br />

“We are still in the eradicative mode<br />

here,” she stressed.<br />

Besides the San Joaquin Valley,<br />

growers in the desert and Coachella<br />

areas have also been keeping the lid<br />

on ACP populations with coordinated<br />

treatments. The Ventura coastal growing<br />

region and Riverside-San Bernardino<br />

citrus have more ACP pressure. A<br />

summary of 224 scouted sites in<br />

California from June 2017 to September<br />

2018 showed that at Ventura’s 47 sites,<br />

87 percent had ACP nymphs present.<br />

In the Riverside San Bernardino region<br />

of the 47 sites, 88 percent were infested.<br />

The 50 sites in the San Joaquin Valley<br />

had zero percent while Coachella’s 45<br />

sites had 8 percent.<br />

Samples<br />

The hot, dry weather in the desert and<br />

San Joaquin Valley growing areas help<br />

harden new flush depriving ACP as<br />

they need soft flush to lay eggs and as<br />

food for the nymphs. Growers or farm<br />

managers are asked to sample for ACP<br />

whenever young flush is present. The<br />

Tamarixia radiate male cisr.<br />

protocol is to sample one flush on ten<br />

trees on each border of a block. If ACP<br />

is found, the grower liaison should be<br />

notified to confirm a find and make<br />

plans for a coordinated treatment.<br />

Grafton-Cardwell said not to rely on<br />

empty yellow sticky traps to determine<br />

if ACP has invaded an orchard, as they<br />

prefer the new flush.<br />

Workshops on sampling for ACP will be<br />

held again this year, Grafton-Cardwell<br />

said.<br />

When growers are asked to participate<br />

in a coordinated treatment they<br />

should respond quickly and use the<br />

most effective product possible. These<br />

treatments are another reason why<br />

ACP levels have been lower in the San<br />

Joaquin Valley, plus growers are also<br />

using pyrethroids to control glassy<br />

winged sharpshooter.<br />

It is important to note that ACP tend<br />

to be found on the border trees of the<br />

blocks. For all insecticide applications,<br />

the borders should be treated before<br />

treating the interior. Research has<br />

shown, Grafton-Cardwell said, that 80<br />

percent of the ACP in a block are on the<br />

border trees. This does not hold true for<br />

young citrus.<br />

Residual Toxicity<br />

In addition to the coordinated<br />

treatments, the residual toxicity of<br />

Continued on Page 32<br />

30 Progressive Crop Consultant May/June <strong>2019</strong>


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May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

31


“<br />

In southern California a total<br />

of 1,127 HLB positive trees<br />

have been removed. Last<br />

year at this time the number<br />

was 501 trees. This shows the<br />

disease is spreading...<br />

“<br />

ACP tamarixia emergence holes.<br />

Continued from Page 30<br />

the pesticide used is important. Broad<br />

spectrum products that have a four plus<br />

week residual include Baythroid, Danitol,<br />

Actara, Admire, Leverage and Agri-flex.<br />

These products come with a warning<br />

that use may cause flare ups of scale or<br />

mites. Insecticides that are selective with<br />

a two to four week residual are Delegate,<br />

Exirel, Fujimite, Movento and Surround.<br />

Materials allowed in organic production<br />

have a residual of less than two weeks.<br />

They include Pyganic, Entrust, oils and<br />

Celite. These need to make direct contact<br />

to be effective and Grafton-Cardwell<br />

recommends two spray applications to<br />

increase chances of control.<br />

The longer the residual, the more effective<br />

the product will be in controlling ACP<br />

as eggs and nymphs are difficult to reach<br />

with a spray and adult ACP can fly in from<br />

untreated areas and not be affected. The<br />

goal is to keep ACP nymphs below 0.5 per<br />

flush. Admire and Platinum gave the best<br />

results.<br />

Biological Control<br />

Biological control, release of the parasite<br />

Tamarixia by California Department of<br />

Food and Agriculture (CDFA) throughout<br />

ACP infested residential sites in southern<br />

California, will continue, Grafton-Cardwell<br />

said. Releases in commercial citrus are not<br />

feasible due to use of spray applications for<br />

other insect pests and timing.<br />

Tamarixia populations build and move<br />

into citrus October-November, after fall<br />

flush.<br />

Control measures buy time for research<br />

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32 Progressive Crop Consultant May/June <strong>2019</strong>


Bio control Foliar application Inspecting leaves<br />

to create a protected tree, and HLB<br />

resistance. Other strategies include<br />

higher density orchards planned for<br />

shorter tree life span, using interference<br />

RNAs to prevent ACP from picking up<br />

the disease and growing citrus under<br />

protective cover.<br />

Pest Control Districts<br />

Judy Zaninovich, Kern County ACP/<br />

HLB grower liaison said residential<br />

finds of ACP were very high 2015-16.<br />

The county pest control district’s pilot<br />

program for residential citrus has<br />

taken out 2,000 trees near sites where<br />

ACP was detected. There are similar<br />

pilot programs in southern California<br />

counties.<br />

In southern California a total of 1,127<br />

HLB positive trees have been removed.<br />

Last year at this time the number<br />

was 501 trees. This shows the disease<br />

is spreading, but also that CDFA is<br />

improving their detection.<br />

Last year, Zaninovich said, the<br />

potential for a late summer spike in<br />

ACP populations was recognized and<br />

coordinated treatments were done.<br />

Knowing there is the potential for an<br />

upswing in ACP at that time, she said<br />

the plan would be repeated this year.<br />

She said there is also evidence that<br />

nighttime applications may be more<br />

effective.<br />

Irrigation Injection<br />

Best practices for application of<br />

systemic pesticide imidacloprid<br />

delivered via irrigation was discussed<br />

by both Sarge Green, director of<br />

Center for Irrigation Technology at<br />

Fresno State and Rick Leonard of<br />

Bayer.<br />

Continued on Page 34<br />

Control Pocket Gophers in Tree Fruit & Nuts<br />

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- Preventative perimeter<br />

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33


Lab Research Foliar application Tamarixia<br />

Continued from Page 33<br />

Distribution optimization is the key.<br />

The goal there is to make sure the<br />

water is in the right place at the right<br />

time. Green said the soil type controls<br />

movement of the material and pore size<br />

dictates movement. Matching water<br />

delivery to the soil type will improve<br />

efficacy of the material applied. Green<br />

noted that regular maintenance and<br />

auditing of the water delivery system is<br />

important in micro and drip systems.<br />

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Leonard supplied some of the basics for<br />

efficient use of imidacloprid delivered<br />

via irrigation. Admire systemic can be<br />

tank mixed with fertilizer, but needs<br />

agitation. In a 12 hour set, the product<br />

should be injected in a one to two<br />

hours period after the first three to<br />

four hours of the set to achieve the best<br />

distribution.<br />

It will take two to three weeks for the<br />

material to move up from the roots into<br />

the trees. The cooler the weather during<br />

that time, the longer it will take to move<br />

throughout the tree. The best strategy of<br />

use is to target the fall flush.<br />

Ventura coastal area growers have a<br />

more difficult time achieving success<br />

with this systemic application, Leonard<br />

said, due to the high clay and organic<br />

matter soils. If the material only reaches<br />

the sub lethal levels for ACP, it invites<br />

resistance.<br />

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34 Progressive Crop Consultant May/June <strong>2019</strong>


MAY/JUNE <strong>2019</strong><br />

VINEYARD REVIEW<br />

In This Issue<br />

36<br />

Grapevine Heat Stress and Sunburn<br />

Management<br />

42<br />

Grapevine Trunk Diseases: Current<br />

Management Strategies<br />

48 Pierce’s Disease and Glassy-winged<br />

Sharpshooter: Still a Threat to California<br />

Viticulture<br />

May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

35


Grapevine<br />

Heat Stress<br />

and Sunburn<br />

Management<br />

By GEORGE ZHUANG | UCCE Fresno County<br />

Heat waves with extreme daily<br />

temperatures are becoming<br />

more and more common in the<br />

San Joaquin Valley (SJV) during the<br />

middle of growing season, e.g., July<br />

and August. In 2017, grape growers in<br />

the SJV experienced two to three weeks<br />

with maximum daily temperature ≥<br />

110 °F. Sunburn with the associated<br />

severe water stress have resulted in<br />

significant yield loss and poor berry<br />

quality at harvest. Berry sugar, organic<br />

acids, anthocyanins, and phenolics<br />

36 Progressive Crop Consultant May/June <strong>2019</strong><br />

all can be impacted by extreme daily<br />

temperatures. Sugar accumulation can<br />

be significantly affected since the leaf<br />

photosynthetic rate starts to decrease<br />

when the canopy temperature passes 30<br />

°C. Under high berry temperature (≥<br />

30 °C), the degradation of organic acids<br />

start to accelerate as well as anthocyanins<br />

and phenolics.<br />

Water Stress<br />

When the heat wave occurs, it usually<br />

also causes grapevine water stress due to<br />

the need of evaporative cooling in order<br />

to lower the canopy temperature. High<br />

daily temperature coupled with severe<br />

water stress will eventually reduce the<br />

berry size and ultimately make the<br />

berry shrivel and raisin (Figure 1. See<br />

page 38). Several vineyard practices can<br />

be adopted by growers to alleviate the<br />

potential damage from the heat wave<br />

and reduce the yield loss as well as the<br />

degradation of berry composition:<br />

Continued on Page 38


May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

37


Continued from Page 36<br />

1<br />

1) Row orientation<br />

2) Trellis selection<br />

3) Variety selection<br />

4) Canopy management<br />

5) Irrigation scheduling<br />

6) Canopy shading<br />

7) Canopy cooling<br />

Row Orientation<br />

The optimum row orientation in the<br />

SJV is southwest to northeast with<br />

approximately 45° angle to have the<br />

equal sunlight exposure on both sides<br />

of the canopy. The traditional row<br />

orientation of raisin vineyard in the SJV<br />

of east to west is still good to minimize<br />

the direct light exposure on fruit-zone.<br />

North to south row orientation should<br />

be avoided for sunburn susceptible<br />

varieties, e.g., Muscat of Alexandria and<br />

Chardonnay.<br />

2<br />

Trellis Selection<br />

Trellis selection is as important as row<br />

orientation. Vertical shoot positioning<br />

trellis is usually not suitable in the SJV<br />

due to the excessive light exposure on<br />

fruit-zone. Two-wire vertical trellis,<br />

or “California Sprawl”, is the most<br />

common and yet suitable for the SJV.<br />

Any trellis with a sprawling system is<br />

preferred under the hot climate.<br />

3<br />

Figure 1. Berry shrivel, raisining, and sunburn of Syrah during the heat wave.<br />

All photos courtesy of George Zhuang.<br />

Varieties<br />

Variety evaluation has been on-going in<br />

University of California (UC) Kearney<br />

REC for a couple of years and the initial<br />

data has confirmed that certain varieties<br />

from southern Mediterranean regions<br />

can tolerate the heat and produce<br />

the decent yield and berry composition.<br />

Some varieties, e.g., Fiano, are under<br />

commercial test to further prove their<br />

suitability under the SJV’s hot climate.<br />

However, the adoption of alternative<br />

varieties might largely depend on marketing<br />

and consumers’ acceptance.<br />

Continued on Page 40<br />

38 Progressive Crop Consultant May/June <strong>2019</strong>


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May/June <strong>2019</strong><br />

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39


Continued from Page 38<br />

4<br />

Canopy Management<br />

Canopy management, e.g., shoot<br />

thinning and leafing, is applied to<br />

provide enough light exposure and<br />

air circulation on fruit-zone without<br />

exposing the clusters to too much direct<br />

sunlight. Hand or mechanical leafing<br />

(Figure 2) can be applied only on the<br />

“morning” side of the canopy to avoid<br />

the afternoon sunlight exposure on<br />

fruit-zone.<br />

5<br />

Irrigation Management<br />

Irrigation management might be the<br />

most critical and powerful tool for<br />

growers and the appropriate irrigation<br />

scheduling can be applied to avoid<br />

excessive heat damage/water stress as<br />

well as berry sunburn. Severe deficit<br />

irrigation should be avoided before the<br />

heat wave occurs to make sure vines<br />

have no or minimal water stress under<br />

the extreme daily temperature. Soil<br />

moisture sensor, pressure chamber, or<br />

basically by feel and appearance can<br />

help growers to assess soil moisture and<br />

vine water status, or growers can simply<br />

follow the grape evapotranspiration<br />

(ET) report (https://ucanr.edu/<br />

sites/viticulture-fresno/Irrigation_<br />

Scheduling/) to decide the amount<br />

of irrigation per week to avoid severe<br />

grapevine water stress during the heat<br />

wave.<br />

6<br />

Canopy Shading<br />

Canopy shading including shade cloth<br />

(Figure 3) and sun protectant, e.g.,<br />

Kaolin and CaCO3 (Figure 4), can<br />

be used to shade the canopy and fruit<br />

to avoid excessive light exposure and<br />

sunburn. Cost and timing might be the<br />

most important factors when growers<br />

decide to use shade cloth and sun<br />

protectant. Generally, the optimum<br />

timing to apply canopy shading is after<br />

berry set or several days before the heat<br />

wave.<br />

7<br />

Canopy Cooling<br />

Canopy cooling can also be applied by<br />

in-canopy misting. Studies in Australia<br />

have found by in-canopy misting it<br />

can cool canopy and clusters by several<br />

degrees, and ultimately improve yield<br />

and berry composition during the heat<br />

wave (https://www.wineaustralia.com/<br />

research/search/completed-projects/<br />

ua-1502).<br />

Integrated Approach<br />

Finally, it might require an integrated<br />

approach by using more than one of the<br />

mentioned strategies to maximize the<br />

production and berry quality during<br />

the heat wave. Growers should consult<br />

local farm advisors and conduct the<br />

small trials to evaluate the effectiveness<br />

of different approaches under the local<br />

condition.<br />

Figure 2. Mechanical leafing at<br />

“morning” side of the canopy during<br />

bloom.<br />

Comments about this article? We want<br />

to hear from you. Feel free to email us at<br />

article@jcsmarketinginc.com<br />

Figure 3. Shade cloth on fruit-zone at “afternoon” side<br />

of the canopy.<br />

Figure 4. Sun protectant of CaCO3 foliar spray during<br />

veraison.<br />

40 Progressive Crop Consultant May/June <strong>2019</strong>


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Grapevine<br />

Trunk Diseases:<br />

Current Management Strategies<br />

By AKIF ESKALEN | Department of Plant Pathology-UC Davis<br />

and JOSÉ RAMÓN ÚRBEZ-TORRES | Agriculture and Agri-Food Canada<br />

Background<br />

Grapevine trunk diseases (GTD) are currently<br />

considered one of the most important<br />

challenges for viticulture worldwide. These<br />

destructive diseases are caused by a broad range of<br />

wood-colonizing fungal pathogens, which primarily<br />

infect grapevines through pruning wounds. In<br />

most occasions, a single vine can be infected by<br />

more than one of these pathogens. The economic<br />

impact of GTD can be significant in both<br />

young and mature vineyards. Characteristic<br />

symptoms include poor vigor, distorted<br />

leaves and shoots, shoot and tendril dieback<br />

and berry specks caused by fungal<br />

toxins produced by some of these pathogens.<br />

Perennial cankers produced by<br />

canker-causing fungi on grapevine cause<br />

spur, cordon and trunk dieback and the<br />

eventual death of the entire vine.<br />

42 Progressive Crop Consultant May/June <strong>2019</strong><br />

Epidemiology<br />

Most of the fungal pathogens responsible<br />

for GTD produce overwintering fruiting<br />

structures containing the spores of the<br />

fungus. When environmental conditions<br />

are favorable, these fruiting bodies release<br />

the spores into the environment. Spores<br />

will land on susceptible pruning wounds and<br />

will initiate infection completing their life cycle. In<br />

California, research suggests that the majority of GTD<br />

spores are released during winter (December to February)<br />

following primarily though not always precipitation events.<br />

GTD fungal pathogens have a broad host range and in<br />

California are known to cause dieback in many different<br />

native or introduced tree species and also in other woody<br />

perennial crops, including tree fruits and nut trees. Therefore, the<br />

source of GTD inoculum (spores) can come into a vineyard from<br />

multiple sources.<br />

Continued on Page 44


REGISTERED MATERIAL<br />

For Use In<br />

Organic Agriculture<br />

Washington State Dept. of Agriculture<br />

May/June <strong>2019</strong><br />

www.progressivecrop.com<br />

43


Continued from Page 42<br />

Figure 1<br />

A B C D<br />

Figure 1. Leaf (tiger stripes) (A), fruit (black measles) (B) and vascular (C) symptoms caused by esca disease<br />

complex. Esca (black measles) and petri disease are primarily caused by the vascular pathogens Phaeomoniella<br />

chlamydospora and Phaeoacremonium minimum, which are also involved in Petri disease in young plants (D).<br />

Figure 2.<br />

In mature<br />

plants, several<br />

basidiomycetes<br />

fungi (primarily<br />

in the genera<br />

Fomitiporia,<br />

Fomitiporella,<br />

Inocutis,<br />

Inonotus, and<br />

Phellinus) play<br />

also a role in<br />

disease and<br />

symptoms<br />

development.<br />

Characteristic<br />

symptoms are<br />

a white rot in<br />

the vascular<br />

system in many<br />

occasions<br />

observed as<br />

a yellowishspongy<br />

wood.<br />

Figure 2<br />

Treat pruning wounds on mother plants to prevent new infections<br />

Management<br />

in Nursery:<br />

Sanitation in mother fields and during the entire nursery process<br />

Disinfect grafting machines regularly<br />

Reduction of the cutting hydration period<br />

Apply control products (chemicals or biologicals) as a dip after<br />

grafting, before storage and/or before dispatch<br />

Hot water treatment of dormant nursery plants prior to dispatch<br />

44 Progressive Crop Consultant May/June <strong>2019</strong><br />

Continued on Page 46


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45


Continued from Page 44<br />

Management<br />

Figure 3<br />

A<br />

B<br />

C<br />

in VINEYARDS:<br />

Use the cleanest plant material available<br />

when establishing new vineyards.<br />

Minimize stress conditions on young<br />

vines after planting.<br />

In California, delayed pruning has<br />

been shown to minimize infection of<br />

pruning wounds as wounds are past the<br />

high disease pressure period of winter<br />

months<br />

Figure 3. Botryosphaeria dieback, commonly known in California as ‘Bot<br />

canker’ is caused by multiple species in the Botryosphaeriaceae family.<br />

Characteristic symptoms are the lack of spring growth of infected areas,<br />

including cordons (A) or spurs (B). Cross sections of infected parts<br />

reveal a wedge-shape canker (C). The GTD disease known as Phomopsis<br />

dieback and primarily caused by the fungus Phomopsis viticola shows<br />

very similar symptoms as Botryosphaeria dieback.<br />

In vertical shoot position (VSP)<br />

systems, double pruning has shown to<br />

facilitate late pruning of large acreage<br />

vineyards and thus, reduce infection.<br />

Prune dead shoots, spurs and cordons<br />

below the symptomatic tissue (at least<br />

a few inches past the last symptomatic<br />

wood).<br />

Make a clean and smooth pruning cut<br />

to speed up the callusing process at the<br />

pruning wound.<br />

Sanitation is very important in<br />

the vineyard. Remove pruned and<br />

infected plant materials to prevent the<br />

development and increase of GTD<br />

fungi overwintering structures in the<br />

vineyard.<br />

A<br />

Figure 4<br />

Figure 4.<br />

Symptoms of<br />

Eutypa dieback,<br />

caused by the<br />

fungal pathogen<br />

Eutypa lata and<br />

several other<br />

Diatrypaceae<br />

species, are<br />

characterized<br />

by distorted and<br />

chlorotic leaves and<br />

short internodes<br />

(A) and by wedgeshape<br />

cankers (B).<br />

B<br />

Protection of pruning wounds with<br />

effective registered chemicals and/or<br />

biological control agents is the most<br />

effective way to prevent new infections<br />

from airborne spores of GTD fungal<br />

pathogens. More than one application<br />

may be necessary to protect the pruning<br />

wound during its susceptible time<br />

period.<br />

Remedial surgery, where visible infected<br />

parts of the vine (spurs, cordons and/or<br />

trunk) are removed, can be an effective<br />

strategy to eradicate the pathogen from<br />

the vine (primarily when cuts are done<br />

lower down on the trunk about 20 to 30<br />

cm above ground) and thus, prolong the<br />

lifespan of vineyards.<br />

Free Access Literature:<br />

Gramaje, D., Úrbez-Torres, J. R., and Sosnowski, M. R. 2018.<br />

Managing grapevine trunk diseases with respect to etiology<br />

and epidemiology: current strategies and future prospects.<br />

Plant Disease 102:12-39.<br />

https://doi.org/10.1094/PDIS-04-17-0512-FE<br />

https://ucanr.edu/sites/eskalenlab/<br />

Comments about this article? We want<br />

to hear from you. Feel free to email us at<br />

article@jcsmarketinginc.com<br />

46 Progressive Crop Consultant May/June <strong>2019</strong>


Science-Driven Nutrition SM<br />

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your crop is getting the nutrients it needs at the right time.<br />

The form of foliar nutrients DO matter. Many foliar nutrient<br />

formulations are built on large chain molecules or unreacted<br />

oxides or carbonates that are not in-solution. These types of foliar<br />

nutrients and others have limited uptake on most leaf surfaces.<br />

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Make sure you know how well your foliar nutrients penetrate.<br />

Ask for SAP analysis comparisons.<br />

The chart shows three Agro-K zinc formulations, Sysstem-ZN,<br />

Zinc Dextro-Lac and CLEAN Zinc were applied to Viognier<br />

grapes on August 8th, along with a commonly available<br />

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47


Pierce’s Disease and<br />

Glassy-winged Sharpshooter:<br />

Still a Threat to California Viticulture<br />

By STEPHEN VASQUEZ | Technical Viticulturist, Sun-Maid Growers<br />

First known as Anaheim grapevine<br />

disease or California vine<br />

disease, Pierce’s disease (PD) has<br />

impacted California’s grape production<br />

since the late 1880’s. Grape<br />

growers had been losing vineyards<br />

to an unidentifiable disease, which<br />

prompted the US Government to hire<br />

Newton B. Pierce, the first United States<br />

Department of Agriculture (USDA)<br />

plant pathologist and namesake of the<br />

disease. Pierce had spent considerable<br />

time walking vineyards in Los Angeles,<br />

San Bernardino and Orange Counties<br />

where Muscat of Alexandria used for<br />

raisins and Mission and other wine<br />

grape varieties were dying at alarming<br />

rates (2). At the time, approximately<br />

25,000 acres had been infected and or<br />

lost to an unknown “malady”. Pierce<br />

also spent time traveling to France, Italy<br />

and other Mediterranean grape growing<br />

regions studying plant disease symptom<br />

expression and declining grapevines<br />

to compare with those found in California.<br />

After researching all aspects<br />

of California viticulture production,<br />

including pests, diseases and associated<br />

symptoms, Pierce could never correctly<br />

identify the cause of California vine disease.<br />

For many years, a plant virus was<br />

thought to be the culprit of PD. It wasn’t<br />

until the mid-1970’s when University<br />

of California (UC) researchers isolated<br />

a bacterium from diseased vines. Once<br />

isolated, the bacteria were reintroduced<br />

to healthy grapevine plants that developed<br />

Pierce’s disease symptoms within<br />

two to four months (1). The bacterium<br />

is known to move from vine to vine<br />

with the help of insect vectors representing<br />

the sharpshooter (Cicadellidae)<br />

and spittlebug (Cercopidae) families.<br />

Infected insects ease of movement into<br />

a vineyard can be devastating in a few<br />

seasons when a PD susceptible grape<br />

variety is planted.<br />

Although Pierce’s disease outbreaks<br />

occurred in California vineyards from<br />

time to time since being ID’d it was not<br />

a primary issue for the grape industry.<br />

Major raisin, table and wine grape<br />

growing regions had moved north<br />

into the San Joaquin and Sacramento<br />

Valleys and the central and north<br />

coast. The mild, southern California<br />

weather was the perfect environment<br />

for the pathogen, vectors and disease<br />

development. In contrast, the<br />

seasonality of the interior valleys and<br />

coastal grape growing regions seemed<br />

to result in a lower PD incidence year<br />

to year. However, there were PD “hot<br />

spots” located near riparian areas<br />

(i.e. Napa and Kings Rivers) or alfalfa<br />

planting that experienced significant<br />

vine deaths. Those hot spots were costly<br />

to individual farming operations but<br />

was not a concern for the industry.<br />

That changed when the disease/vector<br />

dynamics shifted.<br />

In 1999, the nonnative PD vector,<br />

glassy-winged sharpshooter (GWSS),<br />

arrived in Temecula Valley. At that time<br />

a once thriving southern California<br />

wine industry was experiencing rapid<br />

vine death. GWSS turned out to be an<br />

effective vector and superior flyer when<br />

compared to native sharpshooters.<br />

Additionally, vineyards planted<br />

next to citrus proved to be a deadly<br />

combination. GWSS used citrus groves<br />

to feed, breed and for protection from<br />

potential predators from late fall to early<br />

spring. California grape growers were<br />

concerned about their future as they<br />

watched Temecula Valley vineyards<br />

die. As GWSS spread to other parts of<br />

California, PD became a much greater<br />

concern and problem. According to K.P.<br />

Tumber et. al (3) California growers are<br />

48 Progressive Crop Consultant May/June <strong>2019</strong>


paying $56+ million in lost production<br />

and vine replacement annually.<br />

Pierce’s Disease: Cause,<br />

Symptoms and Management<br />

Cause of Pierce’s Disease<br />

A gram-negative bacterium was found<br />

to be the causal organism of Pierce’s<br />

Disease in 1975 (1). Prior to Xylella<br />

fastidiosa being identified, it was<br />

thought that a virus was responsible<br />

for the demise of southern California<br />

vineyards. Newton B. Pierce, the<br />

diseases namesake, began researching<br />

the cause in the late 1880’s but never<br />

properly identified it as a bacterium.<br />

Living in the xylem of grapevines,<br />

X. fastidiosa blocks the movement of<br />

water and nutrients throughout the<br />

plant. Once infected, the bacterium<br />

moves systemically from the point<br />

of infection to other parts of the<br />

plant. Early season symptoms can be<br />

confused with nutrient deficiencies<br />

(i.e. Zinc), displaying interveinal leaf<br />

chlorosis and stunted growth. Late<br />

season symptoms have a scorched<br />

foliage appearance resulting from<br />

the plants inability to move water<br />

through the xylem vessels. Young<br />

vines are more susceptible than older<br />

vines to infection and may die by the<br />

end of the season, while older plants<br />

may display symptoms over several<br />

seasons. However, when bacteria<br />

populations increase to a level that<br />

restricts significant sap movement,<br />

foliage and fruit will dehydrate and die.<br />

Geographical location, time of year<br />

and variety (Table 1. See page 50.) will<br />

determine how severe the symptoms<br />

Photo courtesy of University of CA.<br />

become. As temperatures increase,<br />

fruit will shrivel, and green shoots<br />

mature poorly and never cure prior to<br />

winter. At this point, financial losses are<br />

expected to impact vineyard viability.<br />

Pierce’s Disease Symptoms<br />

• Springtime symptoms consist of<br />

grapevine leaves displaying interveinal<br />

chlorosis<br />

• Late-summer or fall symptoms<br />

consist of grapevine leaves displaying<br />

concentric rings of drying from the<br />

leaf margin towards the center. Leaf<br />

margins of red or black grape varieties<br />

turn red and then brown<br />

• Leaves that have turned brown<br />

will detach, leaving only the petioles<br />

attached to canes<br />

• A unique disease symptom is the<br />

irregular, patchy bark maturity, leaving<br />

half the shoot brown and half green,<br />

displayed as islands of green and<br />

mature brown coloration<br />

• Berries on clusters will shrivel and/<br />

or raisin<br />

Pierce’s disease symptoms can often be<br />

Continued on Page 50<br />

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Table 1. Variety Susceptibility to X. fastidiosa**<br />

Highly susceptible Moderately susceptible PD resistant*<br />

• Chardonnay<br />

• Redglobe<br />

• Fiesta<br />

• Riesling<br />

• Chenin blanc<br />

• Cabernet Sauvignon<br />

• Ruby Cabernet<br />

• Muscat of Alexandria<br />

• Thompson Seedless<br />

• 07355-075 (50% Petite Sirah, 25 % Cabernet Sauvignon)<br />

• 09331-047 (50 % Zinfandel, 25 % Petite Sirah, 12.5 %<br />

Cabernet Sauvignon)<br />

• 09356-235 (50 % Sylvaner, 12.5 % Cabernet Sauvignon,<br />

12.5 % Carignane, 12.5 % Chardonnay)<br />

• 09314-102 (62.5 % Cabernet Sauvignon, 12.5 %<br />

Carignane, 12.5 % Chardonnay)<br />

• 09338-016 (62.5 % Cabernet Sauvignon, 12.5 %<br />

Chardonnay, 12.5 % Carignane)<br />

*UC Davis PD resistant wine grape varieties released in 2017 from Dr. A. Walker.<br />

** This is a partial list of susceptible PD varieties. Contact your local UC farm advisor for a complete list.<br />

Continued from Page 49<br />

confused with nutritional deficiencies,<br />

water related issues or other diseases.<br />

Multiple tissue samples should be<br />

shared with your pest control advisor<br />

(PCA), certified crop advisor (CCA),<br />

local farm advisor or university<br />

plant pathologist to correctly ID the<br />

symptoms. Once properly identified,<br />

a treatment plan can be devised to<br />

improve the vineyard’s health.<br />

Management<br />

Management strategies will depend<br />

on several factors. Insect vector,<br />

grape variety and location will have<br />

a significant impact on the success<br />

of managing PD. The four main<br />

insects that transmit PD are the bluegreen<br />

sharpshooter (Graphocephala<br />

atropunctata), native to coastal<br />

regions near riparian areas; the green<br />

sharpshooter (Draeculacephala<br />

minerva) and red-headed sharpshooter<br />

(Xyphon fulgida), native to interior<br />

valleys; and the glassy-winged<br />

sharpshooter (Homalodisca vitripennis),<br />

a non-native species to California<br />

and the most dominate vector of X.<br />

fastidiosa. It is important to monitor<br />

for sharpshooter insects if PD is to be<br />

managed. Sticky cards, sweep nets and<br />

visual observations of the vineyard<br />

and nearby properties will help in<br />

determining the population size and<br />

what control measures will be needed.<br />

Once identified, properly timed<br />

insecticide applications will help reduce<br />

the population. Vineyards located<br />

in areas where the PD bacterium is<br />

common, and temperatures are mild<br />

will be a challenge at keeping PD<br />

under control. In this case, identifying<br />

and managing the insect vector will<br />

be most important. If GWSS is the<br />

primary vector, insecticide applications<br />

will need to be timely to keep insects<br />

from moving into the vineyard. Citrus<br />

planted next to a vineyard will have to<br />

be sprayed as well to keep populations<br />

in check. Citrus should be visually<br />

checked for adults, nymphs and<br />

eggs. Visit the UC Pest Management<br />

Guidelines online for the most current<br />

insecticide management strategies (4,5).<br />

Locations that have a history of<br />

PD should not be planted to highly<br />

susceptible varieties like Chardonnay<br />

if possible. Finding a more tolerant PD<br />

variety will improve the health of the<br />

vineyard. Newly developed PD resistant<br />

varieties have been released from UC<br />

Davis. These numbered wine grape<br />

selections (Table 1.) can be planted<br />

in areas with a high incidence of PD<br />

and used for blending with traditional<br />

varieties. Unfortunately, there are not<br />

any PD resistant varieties for raisin or<br />

table grape production, but research is<br />

ongoing.<br />

Grapevines showing unusual foliar<br />

symptoms should be taken to your<br />

local UC Cooperative Extension<br />

office for identification. Plant tissue<br />

suspected of having Pierce’s disease<br />

can be sent to a diagnostics lab for<br />

positive identification using molecular<br />

tools. Leaf blades and petioles sampled<br />

from green portions of the cane in the<br />

late summer to early fall will give the<br />

best results. Vineyard insects should<br />

be caught and identified, too. A sweep<br />

net or sticky cards strategically placed<br />

in the vineyard can be used to survey<br />

insect populations in areas displaying<br />

foliar symptoms. Unique insects can<br />

be taken to your local Agriculture<br />

Commissioners office or the California<br />

Department of Food and Agriculture—<br />

Plant Health and Pest Prevention<br />

Services Division for identification.<br />

These first steps are paramount for<br />

developing a management plan.<br />

References<br />

1. Davis, MJ, Purcell, AH, Thomson, SH, 1977.<br />

Pierce’s Disease of Grapevines: Isolation of the<br />

Causal Bacterium.<br />

2. Pierce, NB. 1892. The California vine disease:<br />

a preliminary report of investigations. U.S Dep.<br />

Agric. Div. Veg. Pathol. Bull. 2, 222.<br />

3. Tumber K, Alston J, Fuller K. 2014. Pierce’s<br />

disease costs California $104 million per year.<br />

Calif Agr 68(1):20-29. https://doi.org/10.3733/<br />

ca.v068n01p20<br />

4. UC Pest Management Guidelines – Pierce’s<br />

Disease: Xylella fastidiosa http://ipm.ucanr.edu/<br />

PMG/r302101211.html<br />

5. UC Pest Management guidelines –<br />

Sharpshooters http://ipm.ucanr.edu/PMG/<br />

r302301711.html<br />

Comments about this article? We want<br />

to hear from you. Feel free to email us at<br />

article@jcsmarketinginc.com<br />

50 Progressive Crop Consultant May/June <strong>2019</strong>


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