PeloNews Fall 2019

World's First Medium for Mouse (HSCs)
Hematopoietic Stem Cells for Maintaining
Undifferentiated State
HemEx-TypeA is a serum-free media, which contains Polyvinyl Alcohol
(PVA) and can be used for mouse Hematopoietic Stem Cells
(HSCs).
Features
Optimized for the
expansion of
mouse HSCs, Hematopoietic
Stem
and Progenitor
Cell (HSPCs) obtained
from bone
marrow.
Can do expansion
culture of HSCs,
for more than 1
month in undifferentiated
state.
PVA is used
instead of albumin.
In general, culture media for HSCs contains serum albumin. Such albumin
is required to induce cell division of HSCs. On theother
hand, albumin inhibits stable undifferentiated state.
Widely used purified or recombinant albumin contains small amount of
contaminants,but are tough to remove.
It is reported that such contaminants induce differentiation of HSCs.
Moreover, protein's oxidation reaction inducescellular senescence.
Thefore, albumin needs to be replaced by other chemicals.
Dr. Satoshi Yamazaki at The Institute of Medical Science, The University
of Tokyo reported that PVA can replace albumin (1).
Moreover, PVA is a stable chemical with no oxidation reaction in medium
so aging of HSCs are also suppressed. These advantages help to
do expansion culture of HSCs while maintaining undifferentiated state.
Protocol
Please see the paper
of Nature, 571, 117 -
121 (2019).
Note : For culture,
addition of cytokine
is required.
Reference:
(1) Adam C., et al. Nature, 571, 117 - 121 (2019).
Long-term ex vivo hematopoietic stem cell expansion allows nonconditioned transplantation
(paper is attached).