Broad Street Scientific Journal 2020

Figure 4. Combinations with Varied Concentrations
of Naringenin. Effects on biofilm formation of the
combination of naringenin at full (6.25 µg/mL) and
half (3.125 µg/mL) concentrations with cinnamaldehyde
(13.22 µg/mL). Error bars show 1 SEM. ANOVA
test p < 0.0001* (Table 4). Letters correspond to differences
discerned by Tukey’s HSD Test.
Table 4. ANOVA test conducted on full concentrations
of cinnamaldehyde with varied concentrations
of naringenin (Fig. 4).
Source DF Sum of
Squares
Compound
Mean
Square
F
Ratio
Prob > F
5 0.94506 0.189011 29.7551 <0.0001*
Error 18 0.11434 0.006352
Corr.
Total
23 1.0594
Figure 6. Combinations with Varied Concentrations
of Cinnamaldehyde. Effects on biofilm formation of
the combination of cinnamaldehyde at full (13.22 µg/
mL) and half (6.61 µg/mL) concentrations with naringenin
(6.25 µg/mL). Error bars show 1 SEM. ANOVA
test p < 0.0001* (Table 5). Letters correspond to differences
discerned by Tukey’s HSD Test.
Table 5. ANOVA test conducted on full concentrations
of naringenin tested with various concentrations
of cinnamaldehyde (Fig. 6).
Source DF Sum of
Squares
Compound
Mean
Square
F
Ratio
Prob > F
5 2.21176 0.4423 31.761 <0.0001*
Error 18 0.25065 0.0139
Corr.
Total
23 2.4624
Figure 5. Percent Biofilm Inhibition of Varied Naringenin
Concentrations. Biofilm inhibition percentages
calculated as (control OD - treatment OD)/(control
OD)*100. Error bars show 1 Standard Error of the
Mean (SEM).
Figure 7. Percent Biofilm Inhibition of Varied Cinnamaldehyde
Concentrations. Shows the biofilm
inhibition percentages calculated as (control OD -
treatment OD)/(control OD)*100. Error bars show 1
SEM.
4.3 – Multiple Compounds Cellular Growth Assay
The experiment was conducted to show the growth rate
of the V. harveyi bacteria when treated with combinations
used in biofilm formation assays. Because the growth rates
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