2013 Scientific Report

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VARI | <strong>2013</strong> Embryo/sperm cryopreservation We provide cryopreservation services for archiving and reconstituting valuable mouse strains. These cost-effective procedures decrease the need to continuously breed valuable mouse models, and they provide added insurance against the loss of custom mouse lines due to disease outbreak or a catastrophic event. Mouse embryos at various stages of development, as well as mouse sperm, can be cryopreserved and stored in liquid nitrogen; they can be thawed and used, respectively, by implantation into the oviducts of recipient mice or by in vitro fertilization of oocytes. Rederivation Mice harboring pathogens can negatively affect research results, and they may pass on those pathogens to other mice within the colony. Strain rederivation, by embryo transfer, is a management tool to clean a mouse line from pathogen infection or to import mice into a barrier facility from outside the vivarium. At VARI, any mice imported from an outside research institution are rederived to ensure the specific pathogen-free status of the animals coming in, and also to ensure that our existing research models remain pathogen-free. 21
Kyle A. Furge, Ph.D. Laboratory of Interdisciplinary Renal Oncology Dr. Furge received his Ph.D. in biochemistry from the Vanderbilt University School of Medicine in 2000. Prior to obtaining his degree, he worked as a software engineer at YSI, Inc., where he wrote operating systems for remote environmental sensors. Dr. Furge did his postdoctoral work in the laboratory of George Vande Woude. He joined VARI in June 2001 and was promoted to Assistant Professor in May 2005. Dr. Furge also heads the Kidney Cancer Research Program. From left: Ooi, Petillo, Furge, Dykema Staff Karl Dykema, B.A. Aikseng Ooi, Ph.D. David Petillo, Ph.D. Adjunct Faculty Richard Kahnoski, M.D. Brian Lane, M.D., Ph.D. Bin Teh, M.D., Ph.D. 22
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2013 Scientific Report

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