Dyes & Cell Imaging Reagents

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Product Overview Dyes How to use General procedure of detection of cellular acrolein 1. Prepare 10-30 µM AcroleinRED in fresh medium NOTE: Please optimize concentration of reagent for your experiments. Option: Additional staining such as nuclear staining or immunocytochemistry with antibodies of interest are available 8 Catalog# Description Quantity Supplier FDV-0022 AcroleinRED 0,5 mg Funakoshi Ap3, SHG Imaging Dye Ap3 is a newly synthesized novel molecule for second harmonic generation (SHG) imaging. SHG is a nonlinear optical process, the interaction of two photons with a nonlinear material generates photons with twice the energy. SHG imaging is a powerful tool to visualize cell and tissue structure and function. However, most of dyes for SHG imaging emit strong fluorescence signals in addition to SHG signals. The fluorescence emission from dyes disturbs multimodal imagining as well as SHG signal imaging. Ap3 is the first novel dye designed specifically for SHG imaging with virtually no fluorescence signals, improved photostability and less phototoxicity. Ap3 enables us to detect true SHG signals and realizes multimodal imaging. Catalog# Description Quantity Supplier FDV-0008 Ap3, SHG Imaging Dye 1mg Funakoshi PELOBiotech GmbH, Am Klopferspitz 19, 82152 Martinsried www.pelobiotech.com • email: info@pelobiotech.com Tel: +49 89 517 286 59 0 • Fax: +49 89 517 286 59 88
Product Overview Dyes LiveReceptor AMPAR - Endogenous AMPAR Labeling Reagent Neurotransmitter receptors including glutamate receptors and GABA receptors etc. located on post-synapse in neuronal cells play various roles in brain functions. To understand physiological roles of neurotransmitter receptors, live cell imaging is one of the powerful approaches. Conventional imaging methods on live cellsrelay on a genetically engineered proteins fused with fluorescent proteins such as GFP. However, one serious problem is that the functions and movement of over-expressed neurotransmitter receptors with non-physiological tags are not precisely correlated with endogenous native receptors. The labeling methods for endogenous receptors are desirable to observe physiological functions of receptors. LiveReceptor is the world first reagent series for target-specific receptor labeling. The principle of LiveReceptor is based on ligand-directed acyl imidazole (LDAI) chemistry (ref.1,2). LDAI-based chemical labelling is driven by selective ligand-protein recognition, which facilitates an acyl substitution reaction of labeling reagents on nucleophilic amino acid residues including Lys, Ser and Tyr located near ligand-binding domain. After wash out, the labelled receptors which have free ligand-binding pockets are observed on live cells. 9 Catalog# Description Quantity Supplier FDV-0018A LiveReceptor AMPAR 10μg Funakoshi PolyamineRED - Intracellular Polyamine Detection Reagent PolyamineRED is a reagent for imaging intracellular polyamines specifically without any pretreatment or cell lysis. This can be used for live cells, therefore it is suitable for detection or semi-quantitative analysis of intracellular polyamines. The polyamine species, including putrescine, spermidine and spermine etc. and its acetyl derivatives, are one of the essential class of metabolites which have liner alkyl structure with two or more amines. Polyamines are found in all living organisms with high concentration, from sub-millimolar to millimolar, in the cells. Polyamines have polycationic properties and shows an enormous number of biological functions. For example, polyamines interact with DNA/RNA in the nuclear and regulate gene expression. Polyamines also interact with negatively charged proteins and control its function. The major source of polyamines is an amino acid ornithine. In the case of mammalian, ornithine is converted to putrescine by ornithine decarboxylase (ODC), followed by synthesizing spermindine and spermine. Because ODC is highly expressed in cancer cells, polyamines are considered as one of the cancer marker. Catalog# Description Quantity Supplier FDV-0020 PolyamineRED 0,5 mg Funakoshi PELOBiotech GmbH, Am Klopferspitz 19, 82152 Martinsried www.pelobiotech.com • email: info@pelobiotech.com Tel: +49 89 517 286 59 0 • Fax: +49 89 517 286 59 88
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Dyes & Cell Imaging Reagents

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