SpheroSeev for Enrichment of Hydrogels
SpheroSeev1 is a biopolymer with mechanical properties similar to spidersilk that is used as an additive for improving the mechanical properties, viability, and function of 3D hydrogel models. SpheroSeev is supplied as a stock suspension to supplement materials used to create 3D cellular models. The fibers are easy to handle and can be easily incorporated into any bioink with minimal workflow change.
SpheroSeev1 is a biopolymer with mechanical properties similar to spidersilk that is used as an additive for improving the mechanical properties, viability, and function of 3D hydrogel models. SpheroSeev is supplied as a stock suspension to supplement materials used to create 3D cellular models. The fibers are easy to handle and can be easily incorporated into any bioink with minimal workflow change.
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Seevix Material Sciences<br />
Minrav Building, 4th Floor (JBP)<br />
+972.2.5407888 sales@seevix.com<br />
Gel printing or casting:<br />
1. Print, manually dispense into a mold or a tissue culture<br />
well/plate or use any method to create your 3D model.<br />
2. Optional: It is possible to increase the stiffness and<br />
stability <strong>of</strong> the gel by per<strong>for</strong>ming an additional crosslinking.<br />
For higher cross-linking immerse the gel <strong>for</strong> 30-<br />
60 seconds in 60 mM CaCl2.<br />
3. Cover the model with culture media.<br />
4. Place the seeded plate in a tissue culture incubator or<br />
any other conditions suitable to your work flow.<br />
5.<br />
Figure 1 <strong>SpheroSeev</strong> (green) spread in alginate cellular model aggregation<br />
<strong>of</strong> L929 cell line (blue). Evaluated by fluorescent staining by thi<strong>of</strong>lavin-S.<br />
Scale bar indicates 100 μm.<br />
1. Stern-Tal D, Ittah S, Sklan E. A new cell-sized<br />
support <strong>for</strong> 3D cell cultures based on recombinant<br />
spider silk fibers. DOI: 10.1177/08853282211037781<br />
2. Freeman F E, and Kelly D J. Tuning Alginate Bioink<br />
Stiffness and Composition <strong>for</strong> Controlled Growth<br />
Factor Delivery and to Spatially Direct MSC Fate within<br />
Bioprinted Tissues. Scientific Reports 7, no. 1<br />
(December 6, 2017): 17042.<br />
https://doi.org/10.1038/s41598-017-17286-1.<br />
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