amaxa news #10 - Lonza AG
amaxa news #10 - Lonza AG
amaxa news #10 - Lonza AG
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› Product News<br />
Ordering information<br />
Cell Line Nucleofector ® Kit V Cat. No.: VCA-1003<br />
<strong>amaxa</strong> web information www.<strong>amaxa</strong>.com/jurkat-standard.html www.<strong>amaxa</strong>.com/improved-jurkat<br />
› Product News<br />
new<br />
Expression level of maxGFP ® in Jurkat<br />
E6-1 (ATCC ® TIB-152) cells.<br />
Jurkat E6-1 (ATCC ® ) cells were transfected<br />
with Nucleofector ® Solution V either with<br />
Nucleofector ® Program X-01/X-001 or<br />
X-05/X-005 using 2 µg pmaxGFP. 24 hours<br />
post nucleofection ® , cells were analyzed<br />
on a Becton Dickinson FACSCalibur for<br />
determining maxGFP ® expression level<br />
(X-Mean).<br />
96-well Shuttle ® Protocols and Kits –<br />
Human T Cells, HepG2, SH-SY5Y<br />
› Page 13 › www.<strong>amaxa</strong>.com<br />
4000<br />
3500<br />
3000<br />
2500<br />
2000<br />
1500<br />
1000<br />
500<br />
0<br />
X-Mean<br />
m › Up to 95% efficiency and excellent viability<br />
› Low well-to-well variations<br />
› Preserved functionality (e.g., human T cells)<br />
› Suitable for a variety of substrates, e.g., DNA and siRNA<br />
› Fewer cells required compared to the single cuvette Nucleofector ®<br />
High transfection efficiency and viability in SH-SY5Y<br />
(ATCC ® CRL-2266), HepG2 (ATCC ® HB-8065) and<br />
stimulated human T cells with the 96-well Shuttle ® .<br />
SH-SY5Y, HepG2 cell lines and primary stimulated human<br />
T cells were transfected with the 96-well Shuttle ® System using<br />
the recommended Nucleofector ® Programs and pmaxGFP.<br />
24 hours post nucleofection ® , cells were analyzed on a Becton<br />
Dickinson FACSCalibur with HTS options for maxGFP ®<br />
expression. Cell viability was determined by using the<br />
CellTiter-Glo ® Luminescent Viability Assay (Promega) or<br />
CellTiter ® -Blue Cell Viability Assay (Promega).<br />
100<br />
90<br />
80<br />
70<br />
60<br />
50<br />
40<br />
30<br />
20<br />
10<br />
0<br />
%<br />
X-001 X-005<br />
Jurkat E6-1 Jurkat E6-1<br />
(ATCC ® TIB-152) (ATCC ® TIB-152)<br />
Other re-optimized protocols available<br />
› COS-7 › U-937 › PC-12<br />
Transfection efficiency<br />
For the latest addition of 96-well Shuttle ® and regular Nucleofector ® Kits, please check our website: www.<strong>amaxa</strong>.com<br />
Viability<br />
HepG2 SH-SY5Y Stimulated<br />
(ATCC ® HB-8065) (ATCC ® CRL-2266) human T cells<br />
Ordering information 96-well Nucleofector ® Kit 96 reactions 960 reactions<br />
Primary cells T cells, human, stimulated Human T Cell Cat. No.: VHPA-1002 Cat. No.: VHPA-2002<br />
Cell lines SH-SY5Y Cell Line Kit SF Cat. No.: VHCA-1002 Cat. No.: VHCA-2002<br />
HepG2 Cell Line Kit SF Cat. No.: VHCA-1002 Cat. No.: VHCA-2002
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