amaxa news #10 - Lonza AG

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Leading Transfection Technology
Editorial
“In this study, the amaxa nucleofection ® methodology was used
to achieve transfection efficiencies of up to 65%. The transfection
procedure per se had neither appreciable effects on the activation
state of cells nor on their sensitivity to exogenous TCR stimulation.
This experimental set-up therefore allowed a first functional
analysis of HIV-1 Nef in resting CD4 + T cells.”
Keppler et al. (2006) Journal of Leukocyte Biology, 79:616-627.
Dear Scientist,
For researchers working in the field of gene transfer, efficiency and
viability are of utmost importance. To us, however, it has always
been clear that there existed another core interest – the maintenance
of biochemical cell functionality post transfection. That is
why we chose functionality as a key topic for amaxa news # 10.
Read the Application Note on pages 10 and 11 to learn how
nucleofection ® is optimized to preserve superior cell functionality.
Numerous publications have also dealt with this observation.
The above quote clearly underlines the outstanding status of
Nucleofector ® Technology compared to other transfection methods.
In order to develop applications that will provide cell biologists
broader access to monitor cell signaling events in difficult-totransfect
cells, amaxa has teamed up with Promega. More
information on the first results of this cooperation can be found on
pages 6 and 7.
Of course news # 10 also keeps you updated with the latest additions
to our product portfolio. The last months have seen the
introduction of new Kits and Protocols for Mouse Dendritic Cells
(page 4), Neurons (page 5) and Stem Cells (page 14).
Best regards, Rainer Christine
CEO