amaxa news #10 - Lonza AG

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› Product News
new
Now Completed – amaxa’s Product
Portfolio for the Nucleofection ® of
Mouse Dendritic Cells
Mouse dendritic cells (DC) are a key element in the mammalian immune system, thus understanding
their role is of vital interest for academic research groups and industry. With one Nucleofector ® Kit
and two 96-well Shuttle ® Kits optimized for transfection of mouse dendritic cells, amaxa offers a
unique, ready-to-use product portfolio.
Key benefits › Up to 59% transfection efficiency
100
90
80
70
60
50
40
30
20
10
0
%
96-well Shuttle ®
› More than 90% cell viability
› Excellent preservation of functionality (see also page 10 + 11)
› Low cell numbers required
(2.5 - 5 x 104 cells for the 96-well Shuttle ® , 2.5 x 105 for the standard Nucleofector ® )
Efficiency
Nucleofector ®
Transfection performance.
Mouse DC (Balb/C) were transfected according to the
appropriate Optimized Protocol for nucleofection ® using
pmaxGFP. Cells were analyzed 24 hours post nucleofection ®
by flow cytometry for maxGFP ® expression and viability. Cell
viability is given in percent compared to non-transfected
control.
The Nucleofector ® Kit for mouse DCs is only compatible with Nucleofector ® II Devices (carrying software version S3-7
and higher for the Nucleofector ® II and version S4-4 and higher for Nucleofector ® II, serial version ”S“).
› Page 4 › amaxa news # 10
Functionality post nucleofection ® .
The graph displays functionality of immature mouse DC
(Balb/C mice) post nucleofection ® (Sample) on standard
Nucleofector ® and 96-well Shuttle ® . Analyzed was their ability
to secrete IL-6. Therefore, 2 hours post nucleofection ® , cells
were stimulated by LPS. 22 hours later, functionality was
analyzed by IL-6 specific ELISA and is given in percent compared
to non-transfected control (Control).
Ordering information Mouse Dendritic Cell Nucleofector ® Kit 25 reactions Cat. No.: VPA-1011
Mouse Dendritic Cell (immature) 96-well Nucleofector ® Kit 96 reactions Cat. No.: VHPA-1011
Mouse Dendritic Cell (mature) 96-well Nucleofector ® Kit 96 reactions Cat. No.: VHPA-1012
Mouse Dendritic Cell (immature) 96-well Nucleofector ® Kit 960 reactions Cat. No.: VHPA-2011
Mouse Dendritic Cell (mature) 96-well Nucleofector ® Kit 960 reactions Cat. No.: VHPA-2012
amaxa web information For further information, please contact your local representative or visit www.amaxa.com/mouseDC
Viability
100
90
80
70
60
50
40
30
20
10
0
Mouse DC functionality
%
96-well Shuttle ®
Control Sample
Nucleofector ®