amaxa news #10 - Lonza AG
amaxa news #10 - Lonza AG
amaxa news #10 - Lonza AG
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
[k]<br />
[k]<br />
› Product News<br />
new<br />
Ordering information<br />
Further expression vectors<br />
pmaxCloning Vector –<br />
Strong Expression of Your<br />
Individual cDNA<br />
Do you have difficulties in expressing your specific gene-of-interest?<br />
One crucial aspect for obtaining high expression levels is the selection of an appropriate expression<br />
vector. <strong>amaxa</strong>’s novel pmaxCloning vector promotes strong, constitutive expression of cloned DNA<br />
inserts in mammalian cells. It provides gene expression under the control of a CMV promoter* and<br />
contains a multiple cloning site for cloning purposes. With <strong>amaxa</strong>’s pmaxCloning and pmaxFP ®<br />
vectors for fluorescent protein expression you can choose from a broad variety of expression<br />
plasmids for your specific experimental setup.<br />
*The CMV promoter is covered under the U.S. patents 5,168,062 and 5,385,839 and its use is permitted for research purposes only. Any other use of the<br />
CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA.<br />
Benefit from:<br />
› High expression rates in mammalian cells<br />
› Multiple cloning site for convenient insertion of your gene-of-interest<br />
› Proven performance (same backbone as positive control vector pmaxGFP ® )<br />
Cat. No.: VDC-1040 pmaxCloning<br />
Kan r<br />
pmaxCloning,<br />
2.9 kb<br />
pUC ori<br />
MCS Kpnl Pme I EcoR I EcoR V BamH I Nhe I Pme I Sac I<br />
947<br />
pmaxFP ® -Green-C Cat. No.: VDF-1011<br />
pmaxFP ® -Green-N Cat. No.: VDF-1012<br />
pmaxFP ® -Green-PRL Cat. No.: VDF-1013<br />
P<br />
PCMV<br />
IE<br />
SV40<br />
poly A<br />
chimeric<br />
intron<br />
GG.TAC.CGC.CAT.CAT.GAA.GTT.TAA.ACA.<strong>AG</strong>C.TTG.AAT.TCT.CTA.G<strong>AG</strong>.ATA.TCC.TGC.<strong>AG</strong>A.GAT.CTG.GAT.CCC.TCG.<strong>AG</strong>G.CTA.GCG.CGG.CCG.CGT.TTA.AAC.<strong>AG</strong>A.GCT.C<br />
Hind III Xbal Pst I Xho I Not I<br />
pmaxFP ® -Yellow- C Cat. No.: VDF-1021<br />
pmaxFP ® -Yellow-N Cat. No.: VDF-1022<br />
pmaxFP ® -Yellow-PRL Cat. No.: VDF-1023<br />
<strong>amaxa</strong> web information www.<strong>amaxa</strong>.com/expression-vectors.html<br />
MCS<br />
› Page 15 › www.<strong>amaxa</strong>.com<br />
pmaxFP ® -Red-C Cat. No.: VDF-1031<br />
pmaxFP ® -Red-N Cat. No.: VDF-1032<br />
pmaxFP ® -Red-PRL Cat. No.: VDF-1033
Change language