CV Raif Aziz--April 2011 - Physiology and Biophysics - Case ...

PERSONAL INFORMATION:
Curriculum Vitae
Raif Musa Aziz
Place of Birth: Sao Paulo, Brazil
Address: Av Mendes da Rocha, 357
São Paulo, SP 02227-000 Brazil
Phone: 55-11-2951.7673
E-mail: raifaziz@icb.usp.br, raifaziz@hotmail.com
EDUCATION:
Graduate Studies
03/1994 – 12/1997. Biomedical Sciences Graduate Degree – Medical Modality
University of the State of Sao Paulo – UNESP, Sao Paulo, Brazil.
03/1998 – 07/2000. Master Degree in Sciences - Human Physiology Area
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University
of Sao Paulo, Sao Paulo, Brazil.
Advisors: Professors Gerhard Malnic and Margarida de Mello Aires
Thesis: Peritubular arginine vasopressin regulates bicarbonate reabsorption in cortical
distal tubule via V1 and V2 receptors.
08/2000 – 08/2004. Doctorate Degree in Sciences – Human Physiology Area
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University
of Sao Paulo, Sao Paulo, Brazil.
Advisors: Professors Gerhard Malnic and Margarida de Mello Aires
Thesis: Hormonal regulation of intracellular pH and calcium in colonic epithelial cells:
Role of angiotensin II, arginine vasopressin and atrial natriuretic peptide.

Post-Graduate Studies
09/2004 – 09/2007. Postdoctoral Fellow
Department of Cellular and Molecular Physiology, Yale University School of Medicine.
New Haven, CT, USA
Supervisor: Professor Walter F. Boron
10/2007 – 01/2009. Senior Research Associate
Department of Physiology & Biophysics, Case Western Reserve University School of
Medicine. Cleveland, OH, USA.
Supervisor: Professor Walter F. Boron
PRESENT EMPLOYMENT
02/2009 – ongoing. Assistant Professor (Research and Teaching)
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University
of Sao Paulo, Sao Paulo, Brazil.
EXPERTISE
– Surface/intracellular pH and [Cl - ], performed using a combination of proton selective
microelectrode and two electrode voltage clamp techniques;
– in vivo micropuncture and stopped-flow microperfusion measuring tubular pH (or K + )
and transepithelial potential differences, performed using a double-barreled H + (or K + )
exchange resin/reference electrode;
– Measuring Intracellular pH and calcium using fluorescence microscopy;
– Osmotic water permeability, performed using a video imaging system
– Basic molecular biology techniques;
– Surgical procedures: rats (cannulation of the jugular vein, carotid artery, bladder, and
exposure of the kidney for studying renal function and in vivo microperfusion) and frogs
(to isolate of oocytes for electrophysiology and molecular biology experiments);
– Evaluation of renal function in rats and in humans (for students of medical school);
– Basic cell culture techniques;
– Extensive knowledge in the preparation of micropipettes and ion-sensitive
microelectrodes;

SCHOLARSHIPS
AWARDS
1. 03/1995 – 12/1996. Scientific Initiation Scholarship for undergraduates working in a
research project. Supported by University of the State of Sao Paulo
2. 06/1997 – 12/1997. Junior Instructor Scholarship in Kidney, Gastrointestinal and Liver
Physiology. Supported by University of the State of Sao Paulo, Brazil, 1995-1997.
3. 03/1998 – 07/2000. Master Scholarship for graduate student. Supported by Sao Paulo
Research Foundation FAPESP
4. 08/2000 – 08/2004. Doctorate Scholarship for graduate student. Supported by Sao Paulo
Research Foundation FAPESP
1. 07/2006 – 06/2008. Postdoctoral Fellowship from Heritage Affiliate Research
Committee of the American Heart Association (AHA 0625891-T). Project: Exploring
the identity of the ions transported by the Na + –
-coupled HCO3 transporters
2. 05/2003. Travel Award from International Union for Pure and Applied Biophysics to
attend the XIV International Congress of Biophysics, Buenos Aires, Argentina
3. 10/2001. Travel Award from the American Society of Nephrology to attend the
ASN/ISN World Congress of Nephrology, San Francisco, USA.
PRESENT RESEARCH SUPPORT
07/2009 – ongoing
Thematic Project Research Award from Sao Paulo Research Foundation FAPESP
“Molecular and functional study of membrane transporters”. Coordinator: Professor
Gerhard Malnic. Subproject of my responsibility: “Characterization and regulation of
the NH3/NH4 + transport in Xenopus laevis oocytes”.
LECTURES AND ORAL PRESENTATIONS
1. 04/2011 – Selected Short Communication: “Relative CO2/NH3 permeabilities of several
members of the mammalian Aquaporin family: from AQP0 to AQP9”. Experimental
Biology Meeting 2011 – Ammonia Transport Process Section. Washington DC, USA.
2. 04/2011 – Selected Short Communication: “Relative CO2/NH3 permeabilities of human
RhAG, RhBG, RhCG”. Experimental Biology Meeting 2011 – Ammonia Transport
Process Section. Washington DC, USA.

3. 04/2011 – Invited Presentation: “CO2 and NH3 Transport through Aquaporin and Rh
proteins”. Experimental Biology Meeting 2011 – Gas Channel Symposium. Washington
DC, USA.
4. 10/2009 – Selected Short Communication: “Investigating the Mechanism of CO2
Transport through human”. 41 th American Congress of Nephrology. San Diego, USA.
5. 08/2009 – Invited Presentation: “Transport of gases through aquaporins and Rhesus
proteins”. Department of Public Health, University of Sao Paulo, Sao Paulo, Brazil.
6. 06/2009 – Invited Presentation: “The CO2/NH3 Selectivity of AQP1, AQP4, AQP5 and
Rh proteins and Pathway of CO2 movement through AQP1”. XII Simposium about
transport of electrolytes and Kidney Function NEFRETICO. Ribeirao Preto, Brazil.
7. 10/2007 – Selected Short Communication: “The CO2/NH3 Selectivity of AQP1, AQP4,
and AQP5 and How It is Affected by DIDS”. 40 th American Congress of Nephrology.
San Francico, USA.
8. 04/2007 – Selected Short Communication: “Differential effects of DIDS on the CO2 vs
NH3 permeability of AQP1, AmtB, and RhA”. World Congress of Nephrology, Rio de
Janeiro, Brazil.
9. 08/2005 – Invited Presentation: “Evidence from surface-pH transients that CA IV
enhances CO2 influx in Xenopus oocytes”. Musa-Aziz R. and Boron W.F. XX Annual
Meeting of the Federation of the Brazilian Societies of Experimental Biology. Aguas de
Lindoia, Brazil.
10. 07/2003 – Invited Presentation: “Role of [Ca 2+ ]i and PKC in regulating the biphasic
effect of Angiotensin II on Na + /H + exchanger in epithelial colon cells”. XXI Congresso
da Associação Latino Americana de Ciências Fisiológicas ALACF. Ribeirão Preto,
Brazil.
PUBLICATIONS
Full Papers (* indicates equally contribution)
1. Boron WF, Endeward V, Gros G, Musa-Aziz R, Pohl P. MF. Intrinsic CO2
permeability of cell membranes and potential biological relevance of CO2 channels.
Chemphyschem, 2011 (Epub ahead of print).
2. Chen LM, J Zhao, R Musa-Aziz, MF Pelletier, I Drummond & WF Boron. Cloning and
characterization of a zebrafish cDNA homologue of human AQP1: A bifunctional
water and gas channel. Am J Physiol Comparative Physiol, 299 (5):R1163-74, 2010.
3. Musa-Aziz R, Boron WF, Parker MD. Using fluorometry and ion-sensitive
microelectrodes to study the functional expression of heterologously-expressed ion
channels and transporters in Xenopus oocytes. Methods, 51 (1): 134-145, 2010.
4. Parker MD, Musa-Aziz R, Boron WF. Letter to the Editor “The use of extracellular,
ion-selective microelectrodes to study the function of heterologously expressed
transporters in Xenopus oocytes”. Am J Physiol Cell Physiol. 296(5):C1243, 2009.

5. Musa-Aziz R, Chen L-M, Pelletier MD, Boron WF. Relative CO2/NH3 permeability of
AQP1, AQP4, AQP5, RhAG, and AmtB. Proc. Natl. Acad. Sci. USA 106(13):5406-11,
2009.
6. Musa-Aziz R, Jian L, Chen L-M, Behar K, Boron WF. Concentration-dependent effects
on intracellular and surface pH of exposing Xenopus oocytes to solutions containing
NH3/NH4 + . J. Memb. Biology 228(1):15-31, 2009.
7. Parker MD*/ Musa-Aziz R*, Rojas JD, Choi I, Daly CM, Boron WF. Characterization
of human SLC4A10 as an electroneutral Na/HCO3 cotransporter (NBCn2) with Cl –
self-exchange activity. J Biol Chem. 283(19):12777-88, 2008.
8. Amorim JB, Musa-Aziz R, Lessa LM, Malnic G, Fonteles MC. Effect of uroguanylin
on potassium and bicarbonate transport in rat renal tubules. Can J Physiol Pharmacol.
84(10):1003-10, 2006.
9. Endeward V, Musa-Aziz R, Cooper GJ, Chen LM, Pelletier MF, Virkki LV, Supuran
CT, King LS, Boron WF, Gros G. Evidence that aquaporin 1 is a major pathway for
CO2 transport across the human erythrocyte membrane. FASEB J. 20(12):1974-81,
2006.
10. Musa-Aziz R, Oliveira-Souza M, and Mello-Aires M. Signaling pathways in the
biphasic effect of ANG II on Na + /H + exchanger in T84 cells. J Membr Biol. 205: 49-60,
2005.
11. Musa-Aziz R and Mello-Aires M. Action of ANG II and ANP on colon epithelial cells.
Pflugers Arch. 450: 405-414, 2005.
12. Oliveira-Souza M, Musa-Aziz R, Malnic G, and Mello-Aires M. Arginine Vasopressin
stimulates H + -ATPase in MDCK cells via V1 (cell Ca 2+ ) and V2 (cAMP) receptors. Am.
J. Physiol. Renal Physiol. 286: F402-F408, 2004.
13. Amorim J B O, Musa-Aziz R, Mello-Aires M, and Malnic G. Signaling path of the
action of AVP on distal K + secretion. Kidney Int. 66: 696-704, 2004.
14. Amorim J B O, Bailey M A., Musa-Aziz R, Giebisch G, and Malnic G. Role of luminal
and pH in distal tubule K + secretion. Am. J. Physiol. Renal Physiol 284: F381-F388,
2003.
15. Musa-Aziz R, Barreto-Chaves M L M, and Mello-Aires M. Peritubular AVP regulates
bicarbonate reabsorption in cortical distal tubule via V1 and V2 receptors. Am. J.
Physiol. Renal Physiol 282: F256-F264, 2002.
Manuscript in Preparation
1. Geyer RR, Parker MD, Buron N, Boron WF, Toye AM, Musa-Aziz R. NH3 (and CO2)
permeability of human RhAG, RhBG, and RhCG. (being written)
2. Musa-Aziz R, Geyer RR, Qin X, Boron WF. “Relative CO2/NH3 permeabilities of
several members of the mammalian Aquaporin family: from AQP0 to AQP9”. (being
written)
3. Musa-Aziz R & Boron WF. Evidence from surface-pH transients that Carbonic
Anhydrase II enhances CO2 influx into Xenopus oocytes. (manuscript to be submitted to Am.
J. Physiol Cell Physio)

4. Musa-Aziz R & Boron WF. Evidence from surface-pH transients that Carbonic
Anhydrase IV enhances CO2 influx into Xenopus oocytes. (manuscript to be submitted to
Am. J. Physiol Cell Physiol)
5. Musa-Aziz R*/Geyer RR*, Shaikh S, Tajkhorshid E, Boron WF. Investiganting the
mechanism of CO2 permeation through human Aquaporin 1. (being written)
PUBLISHED ABSTRACTS
1. Musa-Aziz R. NH3 (and CO2) permeability of human RhAG, RhBG, and RhCG. J. Am.
Soc. of Nephrology 21, 2010.
2. Musa-Aziz R*/Geyer RR*, Shaikh S, Tajkhorshid E, Boron WF. Investigating the
Mechanism of CO2 Transport through human AQP1. J. Am. Soc. of Nephrology 20,
2009.
3. Geyer RR, Musa-Aziz R, Boron WF. Proteomics Approach for Identifying the DIDSreaction
Site of human Aquaporin 1. J. Am. Soc. of Nephrology 20, 2009.
4. Boron WF, Zhou Y, Musa-Aziz R. Role of gas channels in the proximal tubule… and
beyond. Proceedings of the XXXVI International Congress of Physiological Sciences 59,
2009.
5. Musa-Aziz R & Boron WF. Evidence from surface-pH transients that DIDS and pCMBS
reduce the CO2 permeability of AQP1 expressed in Xenopus oocytes. J. Am. Soc. of
Nephrology 19, 2008.
6. Skelton L*/Musa-Aziz R*, Boron WF. Reconstituting the carbonic anhydrase activity of
Receptor Protein Tyrosine Phosphatase gama (RPTPgama ), a putative CO2/HCO3 sensor
deleted in renal cell carcinoma. J. Am. Soc. of Nephrology 19, 2008.
7. Musa-Aziz R*, Parker MD*, Boron WF. Evidence from surface [Cl – ] measurements that
human SLC4A10 (‘NCBE’) is a chloride-independent NaHCO3 cotransporter. J. Am.
Soc. of Nephrology 18: P592A, 2007.
8. Musa-Aziz R, Chen L, Boron WF. The CO2/NH3 Selectivity of AQP1, AQP4, and AQP5
and How It is Affected by DIDS. J. Am. Soc. of Nephrology 18: P6A, 2007.
9. Musa-Aziz R, Chen LM, Boron WF. Differential effects of DIDS on the CO2 vs NH3
permeability of AQP1, AmtB, and RhAG. J. Int. Soc. of Nephrology. 18: P39, 2007.
10. Musa-Aziz R, Chen L-M, Boron WF. Evidence from surface-pH transients that AmtB
and RhAG, expressed in Xenopus oocytes, have a substantially lower CO2/NH3
permeability ratio than AQP1. J. Am. Soc. of Nephrology 17: PO1020, 2006.
11. Musa-Aziz, R, Grichtchenko II, Boron W. F. Evidence from surface-pH transients that
CA IV enhances CO2 influx in Xenopus oocytes. J. Am. Soc. of Nephrology 16, 2005.
12. Musa-Aziz, R, Li-Ming C, Boron W. F. Evidence from surface-pH transients that that
AmtB mediates NH3 influx in Xenopus oocytes. J. Am. Soc. of Nephrology 16, 2005.
13. Lu J, Daly CM, Aziz, RM, Boron WF. “Carbonic Anhydrase II (CA II) does not
enhance the activity of the human electrogenic Na/HCO3 cotransporter (hNBCe1-A) in
Xenopus oocytes”. J. Experimental Biology – FASEB 18: A142, 2005.

14. Musa-Aziz R and Mello-Aires M. Arginine Vasopressin has a biphasic effect on Na + /H +
exchanger in epithelial colon cells, via V1 and V2 receptors. J. Am. Soc. of Nephrology
14, 2003.
15. Musa-Aziz R and Mello-Aires M. AVP modulates Na + /H + exchanger in epithelial colon
cells. Nephrology Dialysis Transplantation 18 (4): M4, 2003.
16. Musa-Aziz R and Mello-Aires M. Angiotensin II (ANG II) modulates Na + /H + exchanger
in epithelial colon cells. J. Am. Soc. of Nephrology 13: P0052, 2002.
17. Musa-Aziz R and Mello-Aires M. Effect of Angiotensin II on pHi and [Ca 2+ ]i regulation
in T84. J. Am. Soc. of Nephrology 12: A2928, 2001.
18. Musa-Aziz R and Mello-Aires M. Peritubular Arginine Vasopressin regulates
bicarbonate reabsorption in cortical distal tubule via V1 and V2 receptors. J. Am. Soc. of
Nephrology 11: A0034, 2000.
MENTORING AND TEACHING EXPERIENCE
Mentoring
02/2010 – ongoing
Under graduante student of Pharmacy and Biochemestry School, University of Sao Paulo,
Sao Paulo, Brazil.
Mentee: Caio Willian Zuchi Rodrigues
08/2009 – ongoing
PhD Student
Mentee: Priscilla Morethson
Teaching
Formal Courses
Starting in 05/2011. Kidney Physiology Classes for the students from Veterinary School,
University of Sao Paulo, Sao Paulo, Brazil.
02/2010 – ongoing. Kidney Physiology Classes for the students from School of Medicine,
University of Sao Paulo, Sao Paulo, Brazil.
08/2009 – ongoing. Kidney Physiology Classes for the students from Pharmacy and
Biochemistry School, University of Sao Paulo, Brazil.
08/2009 – ongoing. Gastrointestinal and Liver Physiology Classes for the students from
Nursing School, University of Sao Paulo, Brazil.
08/2009 – ongoing. Respiratory and Kidney Physiology Classes for the students from
Speech and Occupational Therapy School, University of Sao Paulo, Brazil.

REFERENCES
Gerhard Malnic, M.D., Ph.D
Professor Emeritus of Physiology
Department of Physiology and Biophysics
Institute of Biomedical Sciences
University of Sao Paulo
Av Prof Lineu Prestes, 1524
Sao Paulo, SP 05508-900
Phone : 55-11-3091.7280
E-mail: gemalnic@usp.br
Gerhard Giebisch, M.D
Sterling Professor Emeritus of Cellular and Molecular Physiology
Department of Cellular and Molecular Physiology
Yale University
333 Cedar Street
New Haven, CT
06520-8026 USA
Phone: (203) 785.2989
E-mail: gerhard.giebisch@yale.edu
Gerolf Gros, Prof. Dr. med.
Vegetative Physiologie
Zentrum Physiologie OE – 4220 -
Carl-Neuberg-Straße 1
30625 Hannover
Telefon: 0511 532-2735
Fax: 0511 532-2938
E-Mail: gros.gerolf@mh-hannover.de
Joaquim Procopio, M.D., Ph.D
Professor of Physiology
Department of Physiology and Biophysics
Institute of Biomedical Sciences
University of Sao Paulo
Av Prof Lineu Prestes, 1524
Sao Paulo, SP 05508-900
Phone: 55-11-3091.7284
E-mail: procopio@icb.usp.br

Walter F. Boron, M.D., Ph.D
Professor of Physiology
Department of Physiology and Biophysics
Case Western Reserve University School of Medicine
10900 Euclid Avenue
Cleveland, OH
44106-4970 USA
Phone: (216) 368.3400
E-mail: walter.boron@case.edu