Techniques for cryopreservation of individual or ... - Cleveland Clinic

Table II Cryopreservation of small known numbers of sperm
Author Study type Number of Source of sperm Carrier Recovery rate Motility Survival Fertilization
patients
rate rate rate
..........................................................................................................................................................................................................................................................
Cohen et al. (1997) Full published 6 Poor ejaculate Human, mouse and hamster zona 73% 82% NA 50%
*Walmsley et al. (1998) Full published 34 Poor ejaculate, surgically retrieved Human and hamster zonae 75% 67–100% NA 65%
Montag et al. (1999) Full published NA Laser immobilized ejaculated
sperm
Human zona 92% NA 84% NA
Suzuki et al. (1999) Abstract NA NA Hamster zona pellucida 63–70% 54–73% 65–85% NA
Borini et al. (2000) Full published 4 Testicular Human zonae 88% 27% NA 23%
Hsieh et al. (2000a) Full published 16 Ejaculate, surgically retrieved Human and mouse zonae 82% 83% NA NA
Liu et al. (2000) Full published 9 Testicular Mouse zonae 100% 58% 77% NA
Quintans et al. (2000) Abstract NA NA Droplets on plastic dish 90–100% NA NA NA
**Fusi et al. (2001) Abstract 2 Epidydimal Human zona pellucida NA NA NA 57%
Gvakharia and Adamson (2001) Abstract 18 Ejaculate and testicular ICSI pipetts 92% 52% NA NA
Bouamama et al. (2003) Full published NA Normal ejaculate 0.5 ml microdrop 100% ,50% NA NA
Levi-Setti et al. (2003) Full published 10 Ejaculate Human zona 59% 73% NA NA
Cesana et al. (2003) Abstract NA Poor ejaculate Human zona 80–82% 0–***21% NA NA
Sohn et al. (2003) Abstract 10 Poor ejaculate ICSI pipetts 80% NA 8% NA
Desai et al. (2004a) Abstract 4 Epidydimal/testicular Cryoloops 72% NA NA 58%
Desai et al. (2004b) Full published 10 Poor ejaculate Cryoloop 68% 73% NA 67%
Just et al. (2004) Full published 15 Poor ejaculate Volvox globator algae 100% 63% NA NA
Hassa et al. (2006) Full Published NA Normal and poor ejaculate Human zona NA 0–100% NA NA
Isaev et al. (2007) Abstract 18 Poor ejaculate Agarose gel microspheres 70% of
microspheres
78% NA
****Sereni et al. (2008) Full Published 15 Testicular 5 ml drops in a plastic dish 100% 2% NA 18%
*First live birth using hamster/human zona for single-sperm freezing.
**Seventy-six percent of fertilized oocytes cleaved. After ET to two patients in four cycles, one singleton pregnancy resulted.
***Induced motility by Pentoxifylline.
****Seventy-eight percent of fertilized oocytes cleaved and a biochemical pregnancy resulted from transfer of six embryos to three patients.
158 AbdelHafez et al.