Bioengineered Lactobacillus as Next Generation Probiotics
Bioengineered Lactobacillus as Next Generation Probiotics
Bioengineered Lactobacillus as Next Generation Probiotics
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Formulation & Preservation<br />
Fermentation optimization<br />
B<strong>as</strong>ic preservation matrix<br />
• Trehalose, skim milk, and an antioxidant (sodium <strong>as</strong>corbate)<br />
• Transient buffering<br />
Production of highly viable <strong>Lactobacillus</strong><br />
• By freeze (or spray) drying<br />
• >10 10 colony-forming units (CFU)/100 mg powders<br />
Preservation Matrix Components<br />
B<strong>as</strong>ic matrix<br />
(skim milk, trehalose, phosphate buffer, sodium <strong>as</strong>corbate)<br />
Live/total ratio*<br />
upon drying<br />
16%<br />
B<strong>as</strong>ic matrix + polyol + 2nd antioxidant 32%<br />
Skim milk; 2x trehalose; 2x phosphate buffer; 2x sodium<br />
<strong>as</strong>corbate; 2x polyol; 2x 2nd antioxidant<br />
* live/total ratio=CFU per ml/total cells per ml<br />
>90%