Bioengineered Lactobacillus as Next Generation Probiotics
Bioengineered Lactobacillus as Next Generation Probiotics
Bioengineered Lactobacillus as Next Generation Probiotics
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Strain Selection<br />
Primary screening in vitro<br />
• Bacterial identification (by 16S rDNA gene sequence)<br />
• H 2O 2 & lactate production, doubling time, aerotolerance<br />
• Endogenous pl<strong>as</strong>mid<br />
• Self-aggregation (a surrogate marker for in situ colonization)<br />
• Antibiotic resistance<br />
Secondary screening in vitro<br />
• Competition with commensal bacteria and mucosal pathogens<br />
─ Gardnerella vaginalis (& Candida albicans)<br />
• Susceptibility to possible <strong>Lactobacillus</strong> phages<br />
• Bacterial adherence in a human epithelial model<br />
Properties related to bacterial preservation<br />
• Carbohydrate fermentation profile<br />
• % of viable cells during fermentation and after drying<br />
• Vaginal colonization in a NHP model<br />
Suitability for genetic modification<br />
Cell banks made
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