the inhibitory properties of and mode of action of plant essential oils ...

Figure 2.13 The effect of myrtle oil on G. duodenalis trophozoite protein profile
using SDS-PAGE analysis..........................................................................................86
Figure 2.14 The effect of elemi oil on G. duodenalis trophozoite protein profile using
SDS-PAGE analysis...................................................................................................88
Figure 2.15 In vitro viability of G. duodenalis trophozoites incubated with the
constituents of myrtle or elemi oils.............................................................................89
Figure 2.16 The effect of plant oil constituents on G. duodenalis trophozoite protein
profile using SDS-PAGE analysis..............................................................................90
Figure 2.17 The effect of plant oils and their constituents on a 37 kDa G. duodenalis
trophozoite protein using SDS-PAGE analysis..........................................................91
Table 2.5 Quantity One software analysis of the intensity of a 37kDa protein band.92
CHAPTER 3 – Cryptosporidium parvum
Table 3.1 Effect of time and temperature on the spontaneous excystation rate of C.
parvum oocysts........................................................................................................117
Table 3.2 Effect of purification methods and isolate type on the excystation of C.
parvum oocysts.........................................................................................................120
Table 3.3 Effect of time and temperature on C. parvum oocyst spontaneous
excystation................................................................................................................121
Figure 3.1 Effect of different PEOs on spontaneous excystation in C. parvum
oocysts.......................................................................................................................122
Figure 3.2 Effect of palmarosa oil and geraniol on the in vitro spontaneous
excystation rate of C. parvum oocysts over time......................................................124
Figure 3.3 Effect of geranium oil and citronellol on the in vitro spontaneous
excystation rate of C. parvum oocysts over time......................................................125
Figure 3.4 Effect of sweet fennel oil and trans-anethole on the in vitro spontaneous
excystation rate of C. parvum oocysts over time......................................................125
Table 3.4 Hazard Safety Data for the most effective plant oils................................126
CHAPTER 4 – Trypanosoma cruzi
Table 4.1 Minimal inhibitory concentration of PEOs used in experiments..............146
Figure 4.1 Assessment of epimastigote viability and optimal concentration using
MTS at 37°C.............................................................................................................150
Figure 4.2 Assessment of epimastigote viability and optimal concentration using
MTS at 25°C.............................................................................................................151
Figure 4.3 Viable and non-viable epimastigotes of Trypanosoma cruzi as assessed by
TBMM......................................................................................................................152
Table 4.2 Effect of PEO treatment on T. cruzi epimastigote size.............................154
Figure 4.4 Incubation of T. cruzi epimastigotes in vitro with various PEOs............155
Figure 4.5 Incubation of T. cruzi epimastigotes in vitro with various PEOs showing
their effect on morphology........................................................................................156
Figure 4.6 Titrations of all PEOs investigated with T. cruzi epimastigotes in
vitro...........................................................................................................................159
Table 4.3 Inhibition of T. cruzi epimastigote survival by different PEOs................160
Figure 4.7 Titrations of myrtle and elemi PEOs investigated with T. cruzi
epimastigotes in vitro demonstrating morphological alteration................................160
Figure 4.8 Titration of the 2 major constituents of myrtle oil investigated with T.
cruzi epimastigotes in vitro.......................................................................................162
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