Optical Projection Tomography (OPT) - Biomedicum Imaging Unit ...

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Depth of Focus Issues numerical aperture: NA = n sin� depth of focus: �z ~ NA -2 lateral resolution: �r ~ NA -1 high low NA �r �r �z �z Decrease NA: � �r Depth of Focus (good) � �z Lateral Resolution (bad) � �
Depth of Focus Issues numerical aperture: NA = n sin� depth of focus: �z ~ NA -2 lateral resolution: �r ~ NA -1 Reconstruction: Decrease NA: � �r Depth of Focus (good) � �z Lateral Resolution (bad) 3D resolution limited by �r sample size limited by �z
Page 1 and 2: Optical Projection Tomography (OPT)
Page 3 and 4: Motivation
Page 5 and 6: - E.g. DIC does not relate directly
Page 7 and 8: OPT: How It Works
Page 9 and 10: OPT: How it Works Transmission: Flu
Page 11 and 12: OPT: How it Works Same done for eve
Page 13: Depth of Focus Issues
Page 18 and 19: Image Processing
Page 20 and 21: Reconstruction Algorithms “Simple
Page 22 and 23: Reconstruction Algorithms z = z o z
Page 24 and 25: Reconstruction Algorithms Physical
Page 26 and 27: Resolution & Sample Characteristics
Page 28 and 29: Resolution Example: Mouse Embryo 3.
Page 30 and 31: Resolution Example: Single-Cell Ima
Page 32 and 33: Resolution Example: Live Mouse Tiss
Page 34 and 35: Transmission: Whole-mount In Situ r
Page 36 and 37: Fluorescence: Mouse Embryo E10.5 mo
Page 38 and 39: Arabidopsis Silque, heart-stage emb
Page 40 and 41: Mouse Models of Diabetes Normal Dia
Page 42 and 43: Gene Expression Patterning in Mouse
Page 44 and 45: Applications: Live Sample Imaging
Page 46 and 47: The Bioptonics OPT System More OPT
Page 48 and 49: Live Mammalian OPT: Hardware XY tra
Page 50 and 51: Applications: Mouse Limb Developmen
Page 52 and 53: Quantifying Gene Expression & Morph
Page 54 and 55: Quantifying Gene Expression & Morph
Page 56 and 57: Tracking Ectodermal Motion Can only
Page 58 and 59: Tracking Ectodermal Motion Fluoresc
Page 60 and 61: Tracking Ectodermal Motion OPT reco
Page 62 and 63: Tracking Volumetric Growth Tamoxife
Page 64 and 65:
Quantitative Modeling Mesh for a Fi
Page 66:
Acknowledgements Sharpe Lab Jean-Fr
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Optical Projection Tomography (OPT) - Biomedicum Imaging Unit ...

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