Optical Projection Tomography (OPT) - Biomedicum Imaging Unit ...

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Tracking Ectodermal Motion Can only track shape changes Cannot track tissue movements Require Landmarks the model we want to make the data we have (morphology)
Tracking Ectodermal Motion Fluorescent beads attached to limb surface Boot et al, Nat. Methods, 5, pp. 609-612 (2008) raw <strong>OPT</strong> projections 1 time-point; multiple views
Page 1 and 2:
Optical Projection Tomography (OPT)
Page 3 and 4:
Motivation
Page 5 and 6: - E.g. DIC does not relate directly
Page 7 and 8: OPT: How It Works
Page 9 and 10: OPT: How it Works Transmission: Flu
Page 11 and 12: OPT: How it Works Same done for eve
Page 13 and 14: Depth of Focus Issues
Page 15: Depth of Focus Issues numerical ape
Page 18 and 19: Image Processing
Page 20 and 21: Reconstruction Algorithms “Simple
Page 22 and 23: Reconstruction Algorithms z = z o z
Page 24 and 25: Reconstruction Algorithms Physical
Page 26 and 27: Resolution & Sample Characteristics
Page 28 and 29: Resolution Example: Mouse Embryo 3.
Page 30 and 31: Resolution Example: Single-Cell Ima
Page 32 and 33: Resolution Example: Live Mouse Tiss
Page 34 and 35: Transmission: Whole-mount In Situ r
Page 36 and 37: Fluorescence: Mouse Embryo E10.5 mo
Page 38 and 39: Arabidopsis Silque, heart-stage emb
Page 40 and 41: Mouse Models of Diabetes Normal Dia
Page 42 and 43: Gene Expression Patterning in Mouse
Page 44 and 45: Applications: Live Sample Imaging
Page 46 and 47: The Bioptonics OPT System More OPT
Page 48 and 49: Live Mammalian OPT: Hardware XY tra
Page 50 and 51: Applications: Mouse Limb Developmen
Page 52 and 53: Quantifying Gene Expression & Morph
Page 54 and 55: Quantifying Gene Expression & Morph
Page 58 and 59: Tracking Ectodermal Motion Fluoresc
Page 60 and 61: Tracking Ectodermal Motion OPT reco
Page 62 and 63: Tracking Volumetric Growth Tamoxife
Page 64 and 65: Quantitative Modeling Mesh for a Fi
Page 66: Acknowledgements Sharpe Lab Jean-Fr
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Optical Projection Tomography (OPT) - Biomedicum Imaging Unit ...

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