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CTL S6 FluoroSpot Analyzer Line
Immune monitoring moves towards multiplexing. The CTL S6 FluoroSpot analyzer line permits multicolor
ELISPOT analysis, live/dead/apoptotic PBMC counting, and much more. These instruments offer
an attractive alternative to flow cytometry, sharing its strengths while bypassing its weaknesses.
Up to Eight Color
Fluorescent Analysis
Three individually engaged fluorescent
light sources permit selective excitation
of fluorochomes and quantum dots,
even if they have partially overlapping
spectra. When used with the eight customizable
filters, cross contamination
of colors can be avoided: no compensation
needed! These analyzers feature
bottom and top lights for versatile
white light operation.
Versatile Well Formats
These instrument’s wide optical zoom
range permits analysis at the microscopic
(single cells and subcellular
particles) and macroscopic level
(colonies, plaques), in 384-, 96-,
48-, 24-, 12-, and 6-well format. The
readers can also analyze histological
slides and hemocytometers.
ELISPOT Analysis
The primary field of application for S6
FluoroSpot analyzers has been multicolor
ELISPOT analysis. Due to the cross
contamination-free excitation and detection
of fluorochromes, single positive,
or up to eight analyte co-expressing
cells can be accurately detected, including
polyfunctional T cells. Since apoptotic
PBMC are the largest variable in
ELISPOT assay results, these instruments
have been equipped with a live/dead/
apoptotic cell counting module.
These readers enable 384-well
ELISPOT analysis, a novel trend in the
field that permits work with 75% less
PBMC material than the traditional 96well
method.
CTL’s acclaimed ImmunoSpot ®
software assists with all major steps
of the assay. It designs and prints
plate layouts, generates barcodes,
documents raw, counted and QC images,
and exports the counts into an
assigned database to automatically
calculate the results.
Colony and Plaque Counting
The CTL S6 analyzers find increasing
acclaim in the field of colony and
plaque counting.
Due to the complex morphologies
involved, until recently such assays
could only be reliably evaluated
by highly-trained experts. CTL has
developed a sophisticated, automated
image analysis platform, BioSpot ® ,
that permits the automated counting
of such assays with an accuracy
comparable to visual counts generated
by experts. Supported formats are from
384 to 6 wells, using visible and/or up
to eight-color fluorescent analysis for:
• Viral Plaque Assays
• Clonogenic Assays
• Stem Cell assays
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FEATURES AND
SPECIFICATIONS
• Genotoxic Assays
• Microbial Assays
• and more…
Single Cell or Bead Analysis
Flow cytometry does not work well
with adherent/clumping cells, requires
a sample size of more than 100,000
cells for analysis, and is slow and inac-
The Exact Science
CTL S6 FLUOROSPOT ANALYZERS
ULTRA UNIVERSAL ULTIMATE
White light sources 2 2 2
Fluorescent light sources 2 2/3* 3
Number of filters 4 4/8* 8
# of Analytes
Number of non-overlapping
fluorescent analytes
4 4/7* 7
Motorized zoom lens �� �/�* ��
Maximum image resolution
(mega pixels)
Supported plate formats
8 8/25* 25
384-well �� �� ��
96-well �� �� ��
48-well �� �� ��
24-well �� �/�*� ��
12-well �� �/�* ��
6-well �� �/�* ��
Histological Slides �� �/�* ��
Live/dead/apoptotic PBMC
counting �� �� ��
Single cell analysis �� �� ��
Subcellular analysis �� �� ��
* The S6 Universal comes with the basic specifications of the Ultra, but unlike the Ultra, it
has been designed modularly, and is upgradable to specifications of the Ultimate.
curate when it comes to acquiring
rare cells (