here - Nippon Genetics

Dear Readers, 

The first English catalogue of NIPPON GENETICS EUROPE GmbH is in your hands and you may wonder who is behind this name. In order to bring light into the 

darkness, we would like to introduce ourselves briefly. 

NIPPON GENETICS EUROPE GmbH is a subsidiary of NIPPON GENETICS Co., Ltd. (NGC) with its headquarter in Tokyo. The company was founded in 1989 

and specialized in consumables and innovative products for Life Sciences. During the first years NGC made itself a name as a leading distributor, and even today 

products from supplier like Sorenson, Kapa Biosystems, 4titude, Ibidi and others are distributed to the Japanese market. 

Three years ago we started to work with independant manufacturers to produce NGC custom made products exclusively for us. This product range was extended 

recently by many interesting and innovative products. 

Until now, many of these products, are manufactured in Japan, and it is difficult to receive them in Europe or unfortunately too expensive. With the start of 

NIPPON GENETICS EUROPE GmbH this has changed. 

We are a small group of highly motivated and enthusiastic scientists and we look forward to providing you with our advice and assistance in your daily lab work. 

Dr. Oliver Schwarz and Dr. Jürgen Lünzer have more than 30 years of experience in Life Science and we hope we can show you some of our high quality 

products and will have a chance to convince you about our service. 

We look forward to working with you! 

Dr. Oliver Schwarz Dr. Jürgen Lünzer 

About us: 

Nippon GENETICS EUROPE GmbH Nippon GENETICS Co., Ltd. 

Hongo Tsuna Building 17-9 

Binsfelder Strasse 77 Hongo-6-Chome, Bunko-ku 

52351 Düren, Germany 113-0033 Tokyo, Japan 

Phone: ++49-2421-2084690 Phone: ++81-3-3813-0961 

Fax: ++49-2421-2084691 Fax: ++81-3-3813-0962 

www.nippongenetics.eu www.genetics-n.co.jp

PCR Thermo Cycler Page 4 - 5 

Direct PCR reagent and kits Page 6 - 7 

Direct PCR Reagent DNAreleasy Advance Page 6 

FastGene ® Direct PCR kits Page 7 

cDNA libraries Page 8 - 9 

1st strand cDNA libraries from mouse and rat 

PCR plastics 

Page 8-9 

Page 10 - 13 

Single tubes / 8well strips Page 11 

96well PCR plates Page 12 

Adhesive seals Page 12 

PCR Stay Cool Rack Page 13 

Manual DNA Purification Kits Page 14 - 17 

Plasmid Mini DNA Kit Page 15 

PCR DNA Clean-up and Gel Extraction Kit Page 16 

Dye Terminator Removal Kit Page 17 

DNA Electrophoresis Page 18 - 23 

Midori Green DNA dyes Page 18 

Agarose Page 19 

Agarose Gel Band Cutter Page 19 

DNA Marker Page 19 

MUPID Electrophoresis system and accessories Page 20 

MUPID LED Illuminator Page 21 

LED Illuminators Page 22 

UV Transilluminator Page 23 

Protein Electrophoresis Page 24 - 27 

Protein Electrophoresis Chamber Page 24 

Pre Stained Protein Marker Page 24 

Polyacrylamide Pre Cast Gels & Cast System Page 25 - 27 

Cell Biology Page 28 - 29 

Bambanker Cell Freezing Medium Page 28 - 29 

Laboratory Equipment Page 30 

Mini Centrifuge Quiety Page 30 

Homogenisator Mixy Page 30

4 

SuperCycler 

efficient, intuitive, easy handling 

Efficient, intuitive, easy to use: these are the three most common comments from users about the Kyratec SuperCycler. 

The SuperCycler is characterized by a high performance active heating and cooling using quality 8-in Peltier elements, 4 high precision sensors and the latest control 

software. The block system is designed for the use of 96 individual PCR tubes (0.2 ml), 12 PCR 8-well strips (0.2 ml) or 96-well PCR plates (0.2 ml). SuperCycler 

combines the latest electronics and Peltier technology with unusually high operating comfort. 

Powerful Thermal Engine with Gradient ability 

A precision composite alloy block with low mass and high thermal 

conductivity provides excellent ramp rates, long peltier life and low well to 

well temperature variation. 

8 high quality peltier devices with 4 independent control systems and 

feedback sensors allow for high thermal uniformity across the block. 

Compact Footprint 

Boasting a footprint of only 18 x 28.5 x 19 cm (W x D x H), the 

SuperCycler is designed to save valuable bench space within 

the laboratory. 

Weighing in at just 5.5 kg, this machine is also highly portable 

for the ever-changing laboratory environment. 

Touch Screen Graphical User Interface 

A high performance graphical processor with large 7 inch, 

vivid color touch screen display allows for easy run setup 

and monitoring. The powerful yet intuitive software makes 

creation of even the most complex of thermal profiles a 

breeze. Free software upgrades are provided on our website 

keeping your instrument up to date with the latest features 

and developments 

Screenshots 

Start screen 

Load a saved profile from the internal memory or 

USB stick, or create a new profile, by using the 

helpful Wizard assistant 

Wizard Mode 

The Wizard utility enables the user to configure 

easy to moderate complexity profiles in just moments. 

All the thermal steps which occur in a typical 

profile are included and the parameters may be 

adjusted in just a few clicks. 

Heated Lid Evaporation Control 

The SuperCycler employs an applied pressure heated lid 

design to keep the air contained within the tube hotter than 

the reaction volume. This causes any evaporation to condense 

back into the cooler reaction liquid, thereby eliminating the 

need for an oil or wax condensation overlay. 

USB Connectivity 

A front USB port allows for fast, easy file transfer to USB 

memory stick enabling the sharing of thermal profiles 

between instruments and users. The use of a USB mouse is 

also supported. 

Manual Mode 

The Manual Profile editor is very helpful for the 

creation of even difficult profiles. The screen displays 

the current experiment profile in a ‘tree list’ format 

with a graphical representation of each thermal step. 

You can add, modify or delete additional steps easily. 

Phone: ++49/2421/2084690 | Fax: ++49/2421/2084691 | Email: info@nippongenetics.eu 

The User Accounts section 

allows up to 99 user profiles each with dedicated 

file storage directory and personalised 

Icon. When a user is selected thermal profiles 

will be loaded or saved to a directory specific 

to that user providing easy recovery later.

Live Graphing 

Manual Control 

Oligo Calculator 

QuickStart Wizard 

USB Connectivity 

User Accounts 

Pause 

Long Range 

Touch Down/Up 

On Screen Help 

Run Reporting 

SuperCycler 

„Smaller… Faster… Lighter… Cheaper…And perhaps the most user friendly PC cycler ever“ 

Gives vivid feedback of the thermal activity 

Enables the user to set the block to a specific temperature quickly without creating a thermal profile. 

This function is useful for incubating reactions such as DNA digestion or ligation. 

Is t incorporated into the software to assist the operator in oligonucleotide design. 

Enables the user to configure easy to moderate complexity profiles in just moments. 

Front access USB host port enables file transfer between units using an ordinary USB memory 

stick. Also supports the use of a mouse. 

Enables easy separation and organization of user thermal run profiles. Many thousands of 

profiles may be stored in the large internal 256mb+ memory. 

The ‘Pause’ feature allows the user to pause the profile at any number of pre-programmed points 

while emitting an alert beep. 

Enables the time of a particular cycling step to be automatically increased or decreased by a 

preset amount over a specified range of cycle repeats. 

Enables the temperature of a step to be automatically increased or decreased by a preset 

amount over a range of successive cycle repeats. 

User manual is inbuilt into the software ensuring that help is never more than a click away. 

Post run report is generated on run completion and may be saved to USB memory stick for 

inclusion into your run documentation. 

Product Cat.No.: Content 

Specifications 

Technology High performance active heating and cooling using quality peltier elements x8 

and precision sensors x4 

Temperature range 4°C - 99°C 

Temperature Accuracy ±0,25°C 

Temperature Uniformity ±0,3°C 

Temperature Resolution 0,1°C increments 

Heating/Cooling rate 3°C / sec maximum (block) 

Well Configuration 0.2 mL tubes or strip tubes with flat or domed caps; 96-well high-or low skirt plates with strip caps, 

adhesive seal 

Condensation Control Automatic utilising applied pressure heated lid 

Heated Lid Temperature Range 60°C - 115°C 

Dimensions 180 x 285 x 190 mm (W x D x H) 

Weight 5,5 kg 

Electrical 100-240V, 4 Amp (50/60 Hz) Automatic voltage sense, standard IEC Inlet plug 

Interface USB Port for Memory Stick, USB-mouse or keyboard 

Display 7-inch Widescreen Colour Touch Display 

Software Supplied with unlimited user licenses. Free upgrades available via web download 

Functionality Touch Down/Up, LongRange, Program Pauses, On-Screen Help, User accounts, 

Temperature Graphing, Profile Load and Saving, Manual Mode, 

USB File Transfer, post run reporting, and more 

New cycler with 3 individual blocks and 5°/sec ramp rate 

Ordering information 

SuperCycler SC200 High Performance PCR Thermocycler, power cable, User Manual, Touch Screen Stylus 

For further information please visit our web page: www.nippongenetics.eu 

5

6 

DNAreleasy Advance - From cells to PCR in less than 15 minutes 

DNAreleasy Advance is a further development of our well known DNAreleasy lysis reagent, developed for the sample preparation of PCR reactions. 

DNAreleasy Advance is able to replace laborious, expensive and kit based nucleic acid purification kits. The „released“ DNA can be used directly in PCR reactions 

or can be stored at -20°C for future use. 

Further information: 

The following samples were succesfully used already: 

๏ Saliva 

๏ Hair roots 

๏ Animal tissue (horse, pig liver etc.) 

๏ Mouse tails and ears 

๏ Plants (leaf, blossom, pollen): cabbage, maize, canola, soya, sugar beet etc. 

๏ Drosophila 

๏ Yeast 

๏ Mollusca 

Protocol: 

1. Mix cells or homogenized tissue with 30 µl of DNAreleasy Advance in a PCR tube. It is important that the lysis solution covers the cells completely. 

2. NOTE: For Thermal Cycler without heated lid only: Overlay with mineral oil if necessary 

3. Place PCR tube in a Thermal Cycler and start lysis profile: 

Step 1: 65°C for 5 minutes; Step 2: 96°C for 5 minutes; Step 3: 20°C for 5 minutes. 

4. Spin down the remaining tissue if necessary. The DNAreleasy Advance lysate can be used directly in a PCR reaction. DNAreleasy Advance lysate 

could make up to 10% of subsequent PCR mix. We recommend to test different volumes of lysate in the first experiment in order to find the best 

conditions. Residual lysate can be stored at -20°C for future use. 

NOTE: An accurate estimation of DNA yield after lysis can not be done using a spectrophotometer. If PCR results are not satisfying we recommend to 

use FastGene ® Direct PCR Kit. These kits include a special DNA Polymerase providing best results with DNAreleasy Advance 

For research use only 

Rough guide to DNA content of various genomes 

Type Size of DNA (haploid) Weight of DNA (daltons) 1 ng of DNA is included in 

Mammals ~ 3 x 109 ~ 1.9 x 1012 100 cells 

Drosophila ~ 1.2 x 108 ~ 7.7 x 10 10 2.500 cells 

Yeast ~ 1.6 x 107 ~ 1.0 x 10 10 19.000 yeast 

E.coli ~ 4.0 x 106 ~ 2.5 x 109 76.000 bacteria 

Bacteriophage T2 ~ 2.0 x 105 ~ 1.3 x 108 1.500.000 phages 

Bacteriophage ʎ ~ 48.514 ~ 3.1 x 107 6.100.000 phages 

pBR322 ~ 4.363 ~ 2.8 x 106 67.800.000 plasmids 

Phone: ++49/2421/2084690 | Fax: ++49/2421/2084691 | Email: info@nippongenetics.eu

DNAreleasy Advance and all FastGene ® Direct PCR Kits are for research use only 

DNA genotyping of plants with DNAreleasy Advance 

Fig. 1: DNA was isolated from different plant samples with 

DNAreleasy Advance and amplified with the KAPA 2G Robust enzyme. 

(1) Marker 

(2) positive control 

(3) negative control 

(4) white cabbage (leaf) 

(5) sugar beet (leaf) 

(6) maize 

(7) soya 

(8) canola 

(9) wheat 


Product Cat. No. Content 

DNAreleasy Advance (300 µl, 10 rxns) LS05 300 µl DNAreleasy Advance reagent 

DNAreleasy Advance (1,5 ml, 50 rxns) LS06 1,5 ml DNAreleasy Advance reagent 

Fig 2: DNA was isolated from different plant samples with DNAreleasy Advance. Thereafter 

a melting point analysis was performed (Light Cycler, Roche; Switzerland). 

Positive control (PTC), maize, wheat, sugar beet (leaf), white cabbage (leaf), negative 

control (NTC) 

FastGene ® Direct PCR Kit 

is a perfect combination of DNAreleasy Advance and a PCR Ready Mix with a very robust 

polymerase for endpoint PCR. 

Fig. 3: Genomic DNA was released from mussel tissue using 

DNAreleasy Advance reagent and amplified by PCR. The 

agarose gel shows high yields of amplified fragments. 

Data kindly provided by Dr. Schubbert, Eurofins Medigenomix GmbH, Ebersberg, Germany. 

Fig. 4: The amplified DNA from Fig. 3 was sequenced. Due to the high 

PCR efficiency the sequencing profiles of the fragments are exceptional 

satisfying for such short processing time. 

FastGene ® Sturdy and Speedy Polymerase Ready Mixes 

for very difficult templates and for fast PCR even on old thermo cyclers. 

Please ask for more information. Sample kits are available 

FastGene ® Direct PCR Kit (50/100 rxn) LS08 1,5 ml DNAreleasy Advance reagent; 100 rxn of the SturdyPolymerase Ready Mix 

FastGene ® Direct PCR Kit (50/200 rxn) LS09 1,5 ml DNAreleasy Advance reagent; 200 rxn of the SturdyPolymerase Ready Mix 


7

cDNA libraries from mice and rat 

specific organs and development stages 

The Japanese company Genostaff was founded 2002 and is well known as a service provider for pharmaceutical companies all over the world. Their main activities 

are in situ hybridization and construction of c-DNA libraries. The outstanding quality of these custom made products is supported by several hundred of published 

articles in magazines like Nature, Science, Cell and others. 

A specific technology of mRNA isolation allows the construction of cDNA libraries with a very homogenous and exact distribution of all genes. The purified mRNA 

is transcribed with Oligo d(T) primer molecules. The cDNA libraries from Genostaff can be used as a template in PCR (and qPCR), for all experiments in gene 

expression profiling, for the preparation of probes, for cloning experiments etc. 

A wide variety of different cDNA libraries from mouse and rat organs are available. Furthermore embryos of different ages (days) were succesfully used as a template 

for the construction of cDNA libraries. All c-DNA libraries are prepared from the mouse strain BL/6 respectively the Wister Rat. 

1st strand cDNA libraries from mouse and rat 

organs ( adult animals) 

Brain Heart Lung Liver 

Stomach Small Intestine Large Intestine Pancreas 

Skin Muscle Kidney Spleen 

Testis Placenta Ovary Uterus* 

Duodenum* Ileum Jejunum* Brown Fat* 

White Fat* Oculus Spinal Marrow Bone Marrow* 

Prostata* 

Pituitary Gland* 

Thymus Gland* Adrenal Gland* Rectum 

* Products from rat tissue are custom made 

Quality 

Amplification of cDNA by PCR 

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 

M:Marker 

1: Brain 4: Liver 7: Large Intestine 10: Muscle 13: Testis 

2: Heart 5: Stomach 8: Pancreas 11: Kidney 14: Placenta 

3: Lung 6: Intestine 9: Skin 12: Spleen 15: Ovary 

1 µl cDNA (25 ng) from different mouse tissue were applied for PCR. The PCR 

product is a fragment from the GAPDH gene and the size is 436 bp. 

Conditions: 1.25 U Taq Polymerase, PCR Instrument: Applied Biosystems 2700 

3 minutes at 95°C 

30 cycles: 95°C for 30 seconds 

57°C for 30 seconds 

72°C for 30 seconds 

5 minutes at 72°C 

Table: 

2,5 and 25 ng cDNA from different mouse organs and from mouse embryo were applied into 

a PCR reaction (n=3) 

Set up: TaqMan ® FAST PCR Mix,10µL 

TaqMan ® Assay(FAM/MGB)*, 1µL 

Nuclease free water, 8µL 

Mouse GAPD (GAPDH) Endogenous Control (FAM/MGB Probe, Non-Primer Limited), 

Applied Biosystems 

Real Time PCR Instriúment: Applied Biosystems 7500 Fast 

Set up: 20 seconds at 95°C, 40 cycles: 95°C for 3 seconds, 60°C for 30 seconds 

Calibration curve 

1st strand cDNA libraries from 

mouse brain by parts 

Cortex Striatum Olfactoriy Bulb 

Cerebellum Medullary Thalamus+Hypothalamus+Pons 

Hippocampus/Gyrus Dentatus 

1st strand cDNA libraries from mouse 

and rat embryos 

9,5 days 11,5 days 13,5 days 15,5 days 17,5 days 

10,5 days 12,5 days 14,5 days 16,5 days 18,5 days 

R 2 :0.999 

slope:-3.482 

Y-Intercept:25.114 

8 Phone: ++49/2421/2084690 | Fax: ++49/2421/2084691 | Email: info@nippongenetics.eu 

„Mouse Universal Reference Total RNA“(Clontech) and random 

primer molecules were used for the Reverse Transcription PCR. 

This cDNA was used as a standard for calibration. 

Results of PCR amplification by Real Time PCR 

Sample 

(Mouse cDNA) 

Target Yield* 

(Average) 

Yield 

(Std. Dev.) 

Ct 

(Average) 

Ct 

(Std. Dev.) 

Embryo 14.5 days (2.5ng) GAPDH 10.70 0.18 21.53 0.03 

Embryo 14.5 days (25ng) GAPDH 101.36 1.21 18.13 0.02 

Kidney (2.5ng) GAPDH 7.36 0.40 22.10 0.08 

Kidney (25ng) GAPDH 73.80 0.80 18.61 0.02 

Spleen (2.5ng) GAPDH 2.04 0.04 24.04 0.03 

Spleen (25ng) GAPDH 17.12 1.22 20.82 0.11 

Testis (2.5ng) GAPDH 0.61 0.03 25.87 0.07 

Testis (25ng) GAPDH 6.38 0.51 22.32 0.12 

Thymus Gland (2.5ng) GAPDH 1.18 0.03 24.86 0.03 

Thymus Gland (25ng) GAPDH 13.86 0.18 21.14 0.02 

*ng/uL GAPDH cDNA

Ordering information - cDNA organs 

15 reactions per box 

concentration: 25 ng/µl; volume: 15 µl 

Organ Mouse adult 

(BL/6) 

cDNA libraries from mice and rat 

specific organs and development stages 

Cat.No. 

Rat adult 

(Wister) 

Cat.No. 

Brain MD-01 RD-01 

Heart MD-02 RD-02 

Lunge MD-03 RD-03 

Liver MD-04 RD-04 

Stomach MD-05 RD-05 

Small intestine MD-06 RD-06 

Large intestine MD-07 RD-07 

Pancreas MD-08 RD-08 

Skin MD-09 RD-09 

Muscle MD-10 RD-10 

Kidney MD-11 RD-11 

Spleen MD-12 RD-12 

Testis MD-13 RD-13 

Placenta MD-14 RD-14 

Ovary MD-15 RD-15 

Uterus MD-16 RD-16* 

Duodenum MD-17 RD-17* 

Ileum MD-18 RD-18* 

Jejunum MD-19 RD-19* 

Brown fatty tissue MD-20 RD-20* 

White fatty tissue MD-21 RD-21* 

Oculus MD-22 RD-22 

Spinal marrow MD-23 RD-23 

Bone marrow MD-24 RD-24* 

Prostata MD-25 RD-25* 

Thymus gland MD-26 RD-26* 

Adrenal gland MD-27 RD-27* 

Rectum MD-28 RD-28 

Pituitary gland MD-29 RD-29* 

These products are not on stock , custom made products 

Ordering information - cDNA embryo 



Stage Mouse (BL/6) 

Cat.No. 

Rat (Wister) 

Cat.No. 

9,5 days MDE-01 RDE-01 











Ordering information - cDNA brain parts 



Stage Mouse (BL/6) 

Cat.No. 

Cortex MBR-01 

Cerebellum MBR-02 

Olfactory bulb MBR-03 

Hippocampus MBR-04 

Medullary MBR-05 

Striatum MBR-06 

Thalamus+Pons+Hypothalamus MBR-07 



9

PCR plastics 

Optimize your PCR by using the right products - quality „Made in Japan“ 

The performance and reproducibility of your PCR result is significantly influenced by plastics. As a result of a unique manufacturing process our FastGene ® PCR 

single tubes, 8-well-strips and 96-well plates fulfill the highest requests of quality. All FastGene ® PCR plastic products are manufactured by using ultra pure polypropylene. 

Proteins are not able to bind to the surface. The tubes and strips have very thin walls but they are extremely stable and robust. Because of a very stringent 

QC procedure the „batch - to - batch“ reproducibility of all plastics is extremely high. 

FastGene ® PCR plastics stands for: 

Very good contact of single tubes, strips or plates 

to almost all available heating blocks 

๏ Suitable for all thermal cyclers in your lab 

Very fast and homogenous heat transfer from the 

heating block to the sample 

๏ Reproducible PCR results 

Manufactured under stringent clean-room conditions 

๏ Free of RNase, DNase and human genomic DNA 

Precision injection-moulded 

๏ High batch-to-batch reproducibility 

๏ Insignificant evaporation allows small PCR volumes 

Quality Control of FastGene ® PCR plastics: 

One of the problems happened in the past with PCR plastics is the possible evaporation 

of samples. This became very important because more and more users like to perform 

low volume (10 - 15 µl) PCR reactions. 

Therefore the FastGene ® tubes and strips were intensely tested, even under very stringent 

conditions. 

before PCR (5µl) after PCR (5µl) 

Fig. 1: Evaporation test: PCR samples were mixed with a coloured dye. Sample volume: 5 µl · 

Initial denaturation: 95°C; 10 minutes. Cycles: 30 · Denaturation: 94°C; 30 seconds · Annealing: 55°C; 

30 seconds · Extension: 72°C; 60 seconds · Final extension: 72°C; 5 minutes. Total volume was determined 

before and after PCR. 


Manufacturing of FastGene ® PCR plastics in Japan

All FastGene ® tubes and strips have a frosted area for labeling · Unique letters printed on the surface · Suitable for almost all thermal cyclers · 

Reproducible PCR results · Free of RNase, DNase and human genomic DNA · High batch-to-batch reproducibility · 

Insignificant evaporation allows small PCR volumes 

PCR Single Tubes 

PCR plastics 

single tubes and 8-well strips for perfect PCR results 

PCR single tubes 0.2 ml with flat cap (1000) 

Cat.No.: FG-021F 

8-well PCR Strips, Cap Strips and Combi Packs 

0,2 ml PCR 8-well strips without caps (120) 

Cat.No.: FG-028 

0,2 ml PCR 8-well strips and flat cap strips (120) 

Cat.No.:FG-016FC 

8-well PCR Strips with individual caps 

0,2 ml PCR 8-well strips with single flat caps (120) 

Cat.No.:: FG-088WF 

PCR single tubes 0.2 ml with domed cap (1000) 

Cat.No.: FG-021D 

Flat cap strips (120) 

Cat.No.: FG-008FC 

Domed cap strips (120) 

Cat.No.: FG-008DC 

0,2 ml PCR 8-well strips and domed cap strips 

Cat.No.: FG-016DC 

0,2 ml PCR 8-well strips with single domed caps (120) 

Cat.No.: FG-088WD 


11

96-well PCR plates Non-skirted „Easy to cut“ FastGene ® 96-well Plate 

๏ 0,2 ml maximum working volume per well 

๏ Alpha numeric code for sample identification 

๏ DNase, RNase, and human genomic DNA free 

๏ Raised well rims to avoid cross contamination and to facilitate heat sealing 

๏ Compatible with our flat and domed cap strips 

๏ Compatible with heat sealing foils 

๏ Compatible with almost all thermal cyclers 

Cat.No.: FG-170210 (10 plates) 






Self adhesive seals 

Cat.No.: KJ135 (100 sheets) 

Cat.No.: KJ120 (100 sheets) 

PCR plastics 

96-well plates and adhesive seals for perfect PCR results 

This plate is very easy to cut. 

You can cut the plate in 12 x 8 strips 

or 8 x 12 strips or 4 x 24 well plates etc. 

Semi-skirted FastGene ® 96-well Plate 


๏ Black alpha numeric code for easy sample identification 

๏ DNase, RNase and human genomic DNA free 




๏ List of compatible thermal cyclers available at www.nippongenetics.eu 

Full-skirted FastGene ® 96-well Plate 

๏ Low profile plate 


๏ Black alpha numeric code for easy sample identification 

๏ DNase, RNase and human genomic DNA free 




๏ List of compatible thermal cyclers available at www.nippongenetics.eu 

FastGene ® PCR adhesive seal KJ135 

๏ 135 x 80 mm self adhesive seal 

๏ Suitable for Real-Time PCR applications 

๏ Suitable for PE, PS and PP plates 

๏ Extreme high quality prevents evaporation during thermal cycling or 

sample storage 

๏ Peelable without sticky residue present after the seal is peeled off 

๏ End tabs for easy removal 

๏ Resistant to DMSO 

๏ Can be used at temperatures from -80°C to +120°C 

We can offer a price effective foil (KJ 120) for standard applications (non qPCR) 

12 Phone: ++49/2421/2084690 | Fax: ++49/02421/2084691 | Email: info@nippongenetics.eu

Stay Cool Rack 

Cooling rack for PCR tubes with indicator - Safety first for your PCR samples 

The Stay Cool Rack with a lid combines the possibility to cool your samples with the well known 96-well format. This rack can keep the 

temperature below 7°C for 3 - 4 hours, if it was stored at -20°C before. An indicator gel inside the rack will change the colour from purple to 

pink if the temperature is 7°C or higher. This indicator will work for every single well. At the beginning you will see that the outer wells will 

change their colour first, whereas the wells in the centre of the plate still keep a lower temperature. The gel filling is neither toxic nor hazardous. 

FastGene ® Stay Cool Rack 

at -20°C 

Suitable for 0,2 ml PCR Single tubes, 8-well Strips or 96-well plates 

Cat.No.: FG-10 (1 rack) 

Cat.No.: FG-20 (2 racks) 

Cat.No.: FG-015 (500 pcs.) 

Temperature and colour profile 

FastGene ® 1.5 ml reaction tube 

reaction tube for all standard applications in your lab 

FastGene ® 1.5 ml Reaction Tube 

Now available: 1.5 ml FastGene ® Tubes for all standard applications in the lab 

๏ Frosted lid and frosted side writing surface 

๏ Graduations every 100 µl 

๏ Thumb-friendly beveled lip, easy to open and close 

๏ Flat cap accepts lab markers 

๏ Micropestle (see page 30) compatible 

๏ Autoclavable when open 

๏ Compatible with all common micro centrifuges 

FastGene ® Stay Cool Rack 

at room temperature 


13

14 

How much should a NAP kit cost? 

DNA Purification kits 

Manual DNA extraction - high quality and reasonably priced 

The FastGene ® Plasmid Mini Kits and the FastGene ® Gel/PCR Extraction Kits are based on the well known silica membrane / spin column technology: DNA is released 

out of the sample by using modified lysis buffers. Chaotropic salts lead to the destruction of the hydrate shell which normally covers the phosphate backbone 

of the nucleic acid. The sample is applied onto a spin column and the released DNA is bound onto the embedded glass fibre membrane. Thereafter wash steps 

using ethanolic and salt buffers are perfomed in order to remove contaminations. Finally the DNA can be eluted using an aqueous buffer, pH 8, which stabilizes 

the DNA immediately. 

This technology is well known since decades. Unfortunately high quality kits with reproducible results are still expensive. Low priced kits mainly from Asia were 

not able to show reproducible results. 

This will be changed by using our FastGene ® Kits: 

๏ highest quality standard - safety first for your results 

๏ reasonably priced - saving your budget 

๏ interesting additional benefits - unique in the market 

stands for quality „Made in Japan“ 

Learn more about our first FastGene ® Kits: Plasmid Mini Kit, Gel/PCR Extraction Kit and Dye Terminator Removal Kit (based on gel fitration). More Kits will be 

launched quite soon. Please check our web page or subscribe to our newsletter and we will inform you about our new products. 

Plasmid Mini Kit 

(100 / 300 preps) Gel/PCR Extraction Kit 

(100 / 300 preps) 

Dye Terminator Removal Kit 

(50 preps) 


Cat.No.: Product Content 

FG - 90402 FastGene ® Plasmid Mini Kit 100 preps 

FG - 90502 FastGene ® Plasmid Mini Kit 300 preps 

FastGene ® Plasmid Mini Kits 

are designed for rapid small scale isolation and purification of high 

copy and low copy plasmid DNA. The ready-to-use plasmid DNA is of 

high quality in low-salt Tris buffer and suitable for typical downstream 

applications: Cloning, sequencing, PCR, transformation and restriction 

analysis. 


High yield - High purity - Fast preparation 

Comp. Kit A FastGene ® Kit Comp. Kit B 

Fig. 1: pBluescript plasmid DNA was isolated from a 1,4 ml E.coli 

culture (DH5α in LB medium) according to the recommended 

procedures of different kits and eluted in 50 µl elution buffer. 2 µl 

of each eluate were loaded on a 0,7% TAE agarose gel. 

The gel demonstrates that FastGene ® Plasmid Mini Kits yield 

equal amount of plasmid DNA compared to other supplier with 

even more time consuming protocols. The preparation with 

the FastGene ® Plasmid Mini Kit was performed by using the 

Fast Protocol (right side). 


High Quality DNA for DNA Sequencing 

Fig.2: Typical fluorescent capillary sequencing 

result from pBluescript plasmid DNA 

purified using the FastGene ® Plasmid Mini Kit. Cycle 

sequencing reaction was performed on an ABI3100 sequenzer. 

1 µl of purified plasmid DNA was used as a template. Dye terminators 

were removed by FastGene ® Dye Terminator Removal 

Kit (see page 17). 

Plasmid Mini Kit 

High and low-copy plasmid DNA preparation kit 


Parameter High copy Plasmid Low copy Plasmid 

Max. sample volume 1-5 ml ON culture 5-10 ml ON culture 

Typical yield < 25 µg < 25 µg 

Elution volume 50 µl 50 µl 

Binding capacity 40 µg 40 µg 

Size of vector < 15 kb < 15 kb 

Prep time 26 min / 12 samples 36 min / 12 samples 

Format spin column spin column 


๏ Reliable, Fast and Convenient 

๏ Different protocols for different needs 



15

16 


FG - 91202 FastGene ® Gel/PCR Extraction Kit 100 preps 

FG - 91302 FastGene ® Gel/PCR Extraction Kit 300 preps 

FG - 803 FastGene ® AgaroseGel Band Cutter 50 pieces 

FastGene ® Gel/PCR Extraction Kits 

are designed for the extraction of DNA from agarose gels and for the 

purification of PCR products. 

DNA fragments purified with FastGene ® Gel/PCR Extraction Kits 

are ready for direct use in all common downstream applications, like 

sequencing, ligation and transformation, restriction digestion, microarray 

analysis, PCR and in vitro transcription. 


High yield - High purity - Fast preparation 

unpurified Supplier M FastGene ® Kit 

Fig. 1: PCR fragments of 300 bp were purified from 40 µl of a 

PCR stock solution using FastGene ® Gel/PCR Extraction Kit and 

a competitor kit, according to manufacturers protocol. DNA was 

eluted in 20 µl elution buffer and 5 µl of eluate was analyzed on 

a 1,5% TAE agarose gel. 

The figure demonstrates that the FastGene ® Gel/PCR Extraction 

Kit shows up to 90% of DNA recovery. 


DNA Extraction from agarose gels 

1 M 2 3 4 5 6 7 

Fig. 2: 10 µl of a PCR stock solution with a 100 bp amplicon were 

applied on a TBE agarose gel. After electrophoresis the DNA 

was purified using a competitor kit and the FastGene ® Gel/PCR 

Extraction Kit, according to manufacturers protocol. DNA was 

eluted in 20 µl elution buffer and 10 µl of eluate was analyzed on 

a 2% TAE agarose gel. 

The DNA recovery from agarose gels using FastGene ® Gel/PCR 

Extraction Kits is very high even for small fragments. 

Gel/PCR Extraction Kit 

Two in One - DNA Cleanup from agarose gels and PCR reactions 


Parameter Gel Extraction PCR Clean-up 

Max. sample volume 300 mg agarose gel 100 µl PCR mix 

Gel 

< 2,5% TAE or TBE 

Typical Recovery 70-80% 80-90% 

Binding capacity 10 µg 10 µg 

DNA fragment size 50 bp – 10 kbp 50 bp – 10 kbp 

Primer removal 

< 25 bp 

Elution volume 20-50 µl 20-50 µl 

Prep time 20 minutes 20 minutes 

FastGene ® Gel/PCR Extraction Kit 

๏ 2 applications - 1 Kit - no additional buffer needed 

๏ In each kit 5 Agarose Gel Band Cutter and Midori Green 

Advance is included 

All described kits are for research use only 

Lane 1: Unpurified PCR fragment; M = Marker 

Lane 2,3 and 4: Supplier M 

Lane 5,6 and 7: FastGene ® Gel/PCR Extraction Kit FastGene ® Agarose Gel Band Cutter is a ready-to-use disposable tool 

for cutting agarose gel bands easily. It will facilitate your daily work. Five 

cutters are included in every FastGene ® Gel/PCR Extraction Kit. 


in each kit 50µl 

Midori Green Advance 

DNA stain is included

Dye Terminator Removal Kit 

Optimized Sequencing Results 


FG - 9411 FastGene ® Dye Terminator Removal Kit 50 preps 

FastGene ® Dye Terminator Removal Kit 

removes dye terminator molecules from sequencing samples using an 

efficient and reasonably priced gel filtration. The Kit includes a bottle of 

gelfiltration matrix, resuspension buffer and filter spin columns. At first an 

aliquot of the matrix will be mixed with resuspension buffer. Thereafter the 

equilibrated matrix is transferred into filter spin columns. After a brief spin 

up to 20 µl of the sequencing reaction can be loaded onto the column. The 

last centrifugation step will elute the purified sample. All kits can be stored 

at room temperature. 

FastGene ® Dye Terminator Removal 

Optimized Sequencing Results 

Fig. 1: 20 µl sequencing reaction including 2,5 µl BigDye Terminator v3.1 was 

purified with FastGene ® Dye Terminator Removal Kit and analyzed on an ABI 

3100 sequencer. 

The DNA purified by the FastGene ® Dye Terminator Removal Kit shows a very 

good performance in sequencing experiments, no dye blobs occur. 


Parameter Dye Terminator Removal 

Max. sample volume 

Recovery 

Prep time 

Storage 

20 µl sequencing reaction 

> 90% 

5 minutes 

room temperature, 12 months 

FastGene ® Dye Terminator Removal 

๏ cost efficient 

๏ long shelf life at room temperature 



17

18 

Midori Green, Midori Green Advance and Midori Green Direct 

Three non carcinogenic alternative dyes for staining of nucleic acids 

All Midori Green dyes can be used as a safer alternative to the traditional Ethidium Bromide for detecting nucleic acid fragments in agarose gels. 

These newly developed dyes are non carcinogenic and according to the Ames test they cause significantly lower mutation rates than Ethidium Bromide. 

Midori Green and Midori Green Advance can be used for in gel staining as well as for post staining, whereas Midori Green Direct will be mixed just with the samples. 

Midori Green 

The most cost effective non Ethidium Bromide based DNA stain: Just 1,5 -2µl are 

enough for staining of 100 ml agarose gel. 

Fig. 1: Summary of Ames test results: Mutagenicity ratio of Midori Green DNA Stain and Ethidium Bromide with 

metabolic activation (S9) in TA98 mutagenicity assay. According to the results of the Ames test, Midori Green DNA 

Stain is significantly less mutagenic compared to Ethidium Bromide.. 


Midori Green 1 ml MG02 

Midori Green Advance 

Besides the positive effect for your health this dye shows a very high sensitivity even 

for small fragments as well as a superior signal to noise ratio. An unspecific background 

because of non specific binding of dye molecules to agarose is avoided. 4-6 µl are 

enough for the staining of 100 ml agarose gel. 


Midori Green Advance 1 ml MG04 

Midori Green Direct 

Midori Green Direct stain represent a new and safe class of nucleic acid stains for 

visualization of double-stranded DNA, single-stranded DNA and RNA in agarose gels. 

In contrast to most other non Ethidium Bromide based dyes this product is just added to 

your samples. Midori Green Direct includes a 10x sample loading dye. You do not need 

to add any other dyes to both gel matrix and running buffers. 

The DNA sample and Midori Green Direct will be mixed in a ratio of 10:1. Because the 

agarose gel won‘t be stained, the signal noise ratio is excellent. 

Fig. 4: HeLa cells were incubated at 37°C with 1X of SYBR Green I and Midori Green Direct dyes. 

Images were taken following incubation for 30 min. SYBR Green I entered into cells rapidly as evident from 

the bright green nuclear staining. However Midori Green Direct was unable to cross cell membranes as 

shown by the lack of any fluorescence staining. 


Midori Green Direct 1 ml MG06 

1 2 3 4 5 6 1 2 3 4 5 6 

Fig. 2: FastGene ® 100 bp DNA ladder (MWD 100) stained with Midori Green 

(left side, lanes 1 & 6) and Ethidium Bromide (right side, lanes 1 & 6). 

Ethidium Bromide Midori Green Advance 


20µl 10µl 20µl 10µl 20µl 10µl 20µl 10µl 

1,5kb 

1,5kb 

Fig 3: Comparison of sensitivity between Ethidium Bromide (left) and Midori 

Green Advance (right) using an UV-Transilluminator (invers projection). Data 

kindly provided by Ms. Abel, Heinrich-Heine-University Düsseldorf; Germany. 

4µl 3µl 2µl 1µl 0.8µl 0.7µl 

1:2 1:5 1:10 1:25 

-2,8ng 

1 2 3 4 5 6 

1kb 

500bp 

100bp 

Fig.5: Agarose gel analysis of different volumes of 

FastGene ® 1 kb DNA ladder. Even 2 µl of the DNA 

ladder can be detected easily, although 5 µl (0.5 µg) is 

recommended.

This agarose is suitable for the rapid separation of DNA and RNA fragments as well as PCR products 

and plasmid DNA. This molecular biology grade agarose is easy to dissolve and enables a 

rapid gelling. The purity of the agarose leads to an excellent transparency and a low background. 

This results in sharp and well defined DNA and/or RNA bands. 

The high gel strength allows to use concentrations from 0,75% - 2%. Blotting experiments will 

run perfectly and separation efficiency from 250 bp to 23 kb is guaranteed. Every batch of this 

agarose is tested with different sized DNA fragments and the background fluorescence with 

Ethidium Bromide is determined. The presence of DNases and RNases is assayed as well. 

Ask for your free sample of FastGene ® Agarose and check the sharpness of DNA bands especially 

for small fragments (Fig 1): 


FastGene ® Agarose 100 g AG01 

FastGene ® Agarose 500 g AG02 

FastGene ® Agarose Gel Band Cutter 

This easy to use tool will facilitate your daily work. Now you can excise your DNA bands easily. 

FastGene ® Agarose Gel Band Cutter is a ready-to-use and disposable tool for cutting agarose gel 

bands. Please reuse the cutter just for the same DNA/RNA fragments, in order to avoid cross contamination. 

The size of the cutted gel band is 6 mm x 3 mm. 


FastGene ® 

Agarose Gel Band Cutter 

50 pcs. FG-830 

FastGene ® DNA ladder (Molecular Weight Marker) 

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate 

restriction enzymes to yield 12 respectively 13 fragments, suitable for use as molecular weight standards 

for agarose gel electrophoresis. Loading dyes Orange G and Xylene Cyanol FF are already included. 

Product Specifications: 


FastGene ® 1kb ladder 500 µl MWD1 

Agarose 

Range: 100 - 3,000 bp (left side); 250 - 10.000 bp (right side) 

Number of bands: 12 / 13 

Concentration: 100 µg/ml 

Package: 50ug/500ul 

Recommended Load: 5 µl / well 

Stable for at least 6 months at 25°C. For long term storage, store at -20°C. 

Fig 1: Separation of small DNA fragments uisng FastGene ® Agarose and competitor 

products. 

Fig. 2 and 3: The FastGene ® Agarose Gel 

Band Cutter. 


FastGene ® 100 bp ladder 500 µl MWD100 


19

20 

Mupid TM - One Electrophoresis System 

functional design and easy handling 

The Mupid TM - One Submarine electrophoresis system for agarose gels is a further development of the well known Mupid TM - eXu unit, especially for the European 

market. 

What makes this system so popular? 

The Mupid TM - One gel trays are heat resistant: 

By using a novel polymeric material (PPHOX), gel solution up to 100°C can 

be poured into the tray without getting milky or brittle. The clean up of the 

used gel trays can be performed with boiling water. 

What makes this system so convenient? 

The Mupid TM - One ist „multi-sample“ suitable: 

The included combs are multi channel pipette compatible. 

Easy disposal of the running buffer: 

Buffer drains on two corners allow the easy removal of dangerous buffers 

solutions. 

All accessories are included - no extra costs 

Gel casting set ON-MS, included with the MUPID One 

What makes this system so safe? 

For prevention of an electric shock, the system is running only if both parts 

(chamber and power controller) are connected and if the lid is closed! With 

an open lid the main power can‘t be switched on. 

What makes this system so easy to use? 

The power controller can be disconnected easily. Therefore the chamber is 

safe and easy to clean up. 

The power controller is easy to use. Seven conventional voltages (18 V, 

25 V, 35 V, 50 V, 70 V, 100 V and 135 V) are available. Peak voltage is 

constant (140 V) and output level changes through pulse control. A timer 

including an alarm function is available. All parameters of the last run are 

memorized and automatically saved. 

Gel casting set GM-HR, available as a separate item 

The Mupid TM - One comes with these accessories: 

Gel casting set (Cat.No. ON-MS) including 4 combs. The combs can be used from both sides (13 wells and 26 wells). 

Two gel trays small (S) for the preparation of mini gels with one comb (13 or 26 samples) 

One gel tray large (L) for the usage of up to 4 combs (up to 26 x 4 samples). The maximum lenght of separation for the use of 4 combs is 2.7 cm. 


The Mupid TM - One LED Illuminator 

DNA/RNA electrophoresis under „real time“ conditions 

Fig 1-3: The MUPID One LED Illuminator replaces the lid of the MUPID One electrophoresis chamber. 

Now you can follow the separation of your DNA fragments „live“ in agarose gel electrophoresis 

The MUPID One LED Illuminator was developed especially for the MUPID electrophoresis system and allows the detection of DNA fragments during the 

run. The electrophoresis can be continued because the LED illuminator, as shown above, can replace the lid of the MUPID chamber. The orange coloured 

filter protects you, so you can check the results without wearing goggles. The LED source inside the instrument produces light with a narrow emission peak at 

approximately 470 nm, effective for the excitation of nucleic acid stains such as Midori Green (see page 18) and SYBR dyes. The viewing area is coated in order 

to avoid the formation of moisture. The separated DNA is not damaged by the light emitted by LEDs, because it contains no short-wave UV light. Sensitivity is at 

2 ng DNA. The MUPID One LED Illuminator is compatibe ONLY with the MUPID electrophoresis system. 

MUPID One LED Illuminator - Specifications 

Size 166 x 170 x 51 mm 

Viewing area 150 x 60 mm 

Weight 0,9 kg 

LED wave lenght 470 nm 

Filter orange 

Compatible to MUPID One, Mupid exU and Mupid ACE 

Sensitivity ~ 2 ng 



MUPID TM - One electrophoresis system MU2 Mupid TM - One electrophoresis system with 1x gel chamber, 1x power controller, 1x gel casting set, 

4x combs, 2x gel trays S, 1x gel tray L 

MUPID One LED Illuminator MU4 MUPID One LED Illuminator 

Accessories 

Gel casting set large for the Mupid TM - One GM-HR 1x Mupid TM - One gel casting set, 2x combs, 4x gel trays S, 2x gel trays L 

Gel casting set standard for the Mupid TM - One ON-MS 1x Mupid TM - One gel casting set, 4x combs, 2x gel trays S, 1x gel trays L 

Large gel trays for the Mupid TM - One (2) ON-GL 2 gel trays L for the Mupid TM - One electrophoresis system 

Small gel trays for the Mupid TM - One (4) ON-GS 2 gel trays S for the Mupid TM - One electrophoresis system 

Gel casting stand for the Mupid TM - One ON-SD 1 gel casting stand for the Mupid TM - One electrophoresis system 

Gel combs for the Mupid TM - One (2) ON-C1 2 combs for the Mupid TM - One electrophoresis system 

Fig 4: LED‘s of the MUPID One LED Illuminator 


21

22 

LED Illuminators 

the gentle detection of DNA/RNA fragments in agarose gels 

Applikationen : Genexpression / Probe Herstellung / Klonierung (15 Reaktionen) Maus: BL/6 Ratte: Wister 

Fig 1-3: FastGene ® LED Illuminator, Cat.No.: FG-05 

The FastGene ® LED Illuminator and the FastGene ® LED Transilluminator are using blue LED‘s. Light from the LED source inside the instrument produces light 

with a narrow emission peak centered at approximately 470 nm, effective for the visualization of nucleic acid stains such as Midori Green (see page 18) and SYBR 

dyes. Whereas UV light shows the highest sensitivity with dyes like Ethidium Bromide, the longer wavelenght lead to perfect results with the new dyes mentioned 

above. The biggest advantage for the usage of these LED instruments is the fact that the light does not affect skin and eyes and most important the DNA won‘t be 

damaged at all. This is especially important if the excised DNA fragment should be used for cloning experiments. All instruments exhibit an orange coloured filter 

which allows the examination of the separated DNA without any goggles. 

FastGene ® LED Illuminators - Specifications 

Product FastGene ® LED Illuminator FastGene ® LED Transilluminator 

Size 210 x 210 x 30 mm 280 x 340 x 80 mm 

Working area 120 x 70 mm 200 x 160 mm 

Weight 2,3 kg 3 kg 

LED wave lenght 470 nm 470 nm 

Filter orange orange 

LED position aside, above working area aside, below working area 

Fig. 5-7: FastGene ® LED Transilluminator, Cat.No.: FG-06 

The above mentioned FastGene ® LED Illuminator is a smalll and compact instrument but the maximum gel size could not be larger as 120 x 70 cm. The LED 

light comes from aside and above the working area. 

The FastGene ® LED Transilluminator is suitable even for larger gels (up to 200 x 160 mm). The LEDs are positioned aside and below the working area. The 

intensity of the exposure can be varied between low and high. For the detection of DNA/RNA in agarose gels we strongly recommend the combination of the 

FastGene ® LED instruments with a dye like Midori Green, Midori Green Advance or Midori Green Direct (see page 18). 

Product Cat. No.: 


Content 

FastGene ® LED Illuminator FG-05 FastGene ® LED Illuminator 

FastGene ® LED Transilluminator FG-06 FastGene ® LED Transilluminator 


Fig. 1: TAE agarose gel (1%) stained with Midori Green (in 

gel staining) and visualized on the FastGene ® LED Illuminator 

(left picture) and on a regular UV trans illuminator (right picture). Data kindly 

provided by H. Tourné, Institut für Mikrobiologie II, ZMB Essen, Germany.


UV Transilluminator 

highest sensitivity and best protection against UV radiation 

Fig. 1: LED and UV Illuminators from Nippon Genetics Europe Fig. 2: The FastGene ® UV TransiIlluminator 

For the detection of DNA, stained with Ethidium Bromide, UV-Transilluminators are still the first choice. The achievable sensitivity is strongly dependent on the 

quality of the UV lamps and of the filter material. High quality UV tables show almost no visible light. The quality of the UV light source and of the filter can easily 

be tested: UV light is invisible to the human eye. When the instrument is switched on and the position of the UV lamps are easy to detect, too much of the long 

wavelenght light will be emitted and the result is a much lower sensitivity (see figure 3). 

The FastGene ® UV Transilluminator passes this test without a problem 

. Furthermore the FastGene ® UV Transilluminator includes a specifically manufactured filter system and shows a very effective protection against harmful UV 

radiation (see figure 5). 

The combination of high-quality UV lamps and optimized filter material allow the highest sensitivity for the detection of nucleic acids. 

Try the difference today and ask for a demonstration on side 

Fig. 3: UV light source of a conventional device (left) and the FastGene ® UV TransiIlluminator (right) 

FastGene ® UV Transilluminator - Specifications 

Size 280 x 340 x 80 mm 

Working area 260 x 210 mm 

Weight 4,3 kg 

UV wave lenght 302 nm 

UV lamps 6 x 8 Watt 

Power 100 - 240 V; 50 - 60 Hz 

UV transmissibility 0,01% 


FastGene ® UV Transilluminator FG-300 FastGene ® UV Transilluminator 

Fig. 4: Agarose gel with different concentrations of a DNA standard. 

Left: conventional UV transilluminator; Right: FastGene ® UV TransiIlluminator. 

Fig. 5: Permeability of the filter for UV radiation. Blue: conventional UV 

transilluminator, in red: FastGene ® UV TransiIlluminator. 


23

Protein Electrophoresis System 

compact, economical and exclusively available from Nippon Genetics 

The FastGene ® Protein Electrophoresis chamber was developed specifically for the use of precast PAA (10 x 8 cm) gels for protein electrophoresis. It is made of 

highly pure, crystal-clear plastic, which facilitates the application of the sample and allows good visual control during electrophoresis. It can be run simultaneously 

with two gels. The assembly of the gels into the chamber is facilitated by using an innovative „wedge system“ that prevents leakage of the buffer reservoir. During 

electrophoresis, the gel cassettes from both sides are surrounded with running buffer to allow for efficient cooling and thus sharp protein bands. The chamber is 

manufactured according to CE directives. 

FastGene ® Protein Electrophoresis System 

Dimensions 8 cm (L) x 18 cm (W) x 18 cm (H) 

Weight (without gels) 0.5 kg 

Plastics Acryl 

Gaskets Silicon 

Capacity of the buffer chamber 500 ml 

Voltage 0-600 Vdc 

Current 0-100 mA 

Temperature range 0-50°C 

Compatibility to pre cast gels 10 x 8 cm gels; IDMax-gels and others 

Protein Marker - prestained in Pink or Blue 

The Pink Prestained Protein Marker shows 8 pink and 2 blue reference bands in a size range of 15 – 175 kDa. 

The BlueStar Prestained Protein Marker is a 3-colour-protein marker with 10 pre stained proteins in a size range of 10 – 180 kDa. 8 proteins are coupled with 

a blue chromophore. The 25 kDa reference protein is coupled with a green dye, whereas the 75 kDa reference protein is coupled with a red dye. The Blue Star 

prestained protein marker is free of ß-mercaptoethanol. The markers are suitable for all common buffer systems and are supplied ready for use in a loading buffer. 

Furthermore the BlueStar prestained Protein Marker is characterized by high efficiency in Western blot experiments onto PVDF, nylon- or nitrocellulose membranes. 



FastGene ® Electrophoresis Unit FG02 1 FastGene ® Electrophoresis chamber; lid with power cables, anode buffer chamber, the cathode buffer 

chamber with electrodes, gaskets, wedges and two acrylic plates 

Pink prestained Protein Marker (2 x 500 µl) MWP02 2x 500 µl Pink prestained Protein Marker in loading buffer; sufficient for 100 mini gels or 50 large gels 

BlueStar prestained Protein Marker (500 µl) MWP03 1x 500 µl BlueStar prestained Protein Marker in loading buffer; sufficient for 100 mini gels or 50 large gels 


IDSol Sample - and Running buffer for protein gel electrophoresis 

IDSol Running and sample buffers are available as ready solutions or as-measured powder in the aluminum foil to make 1 liter of buffer. 

This eliminates the tedious weighing of SDS and other components. 


Product Cat.No.: Content: 

IDCast Plastic Cassette 10x10 (40) IDC002 40 IDMax empty cassettes, 10x10 cm 

IDCast Plastic Cassette 10x8 (40) IDC001 40 IDMax empty cassettes, 10x8 cm 

Clamps and Combs 10-wells (2) IDC008 2 Clamps and 10-well combs for IDMax empty cassettes 

Clamps and Combs 15-wells (2) IDC009 2 Clamps and 15-well combs for IDMax empty cassettes 

10x Running Buffer Tris-Glycine-SDS (500 ml) ID1501 500 ml 10x Tris-Glycine-SDS Running Buffer 

10x Running Buffer Tris-Glycine (500 ml) ID1511 500 ml 10x Tris-Glycine Running Buffer 

50x Running Buffer Tris-Acetate-EDTA (500 ml) ID1521 500 ml 50x Tris-Acetate-EDTA Running Buffer 

10x Running Buffer Tris-Borate-EDTA (500 ml) ID1531 500 ml 10x Tris-Borate-EDTA Running Buffer 

10x Running Buffer Tris-Tricine-SDS (500 ml) ID1541 500 ml 10x Tris-Tricine-SDS Running Buffer 

Running Buffer Tris-Glycine-SDS Components (10) IP2501 10 pouches with all ingredients for each 1 L Tris-Glycine-SDS Running Buffer 

Running Buffer Tris-Glycine Components (10) IP2511 10 pouches with all ingredients for each 1 L Tris-Glycin Running Buffer 

Running Buffer Tris-Borate-EDTA Components (10) IP2531 10 pouches with all ingredients for each 1 L Tris-Borat-EDTA Running Buffer 

Running Buffer Tris-Tricine-SDS Components (10) IP2541 10 pouches with all ingredients for each1 L Tris-Tricine-SDS Running Buffer 

Running Buffer MOPS-SDS Components (10) IP2551 10 pouches with all ingredients for each 1 L MOPS-SDS Running Buffer 

Running Buffer MES-SDS Components (10) IP2561 10 pouches with all ingredients for each 1 L MES-SDS Running Buffer 

5x Tris-Glycine-SDS Loading Buffer – DTT (10 ml) ID1614 10 ml 5x Tris-Glycine-SDS Loading Buffer, DTT in a separate tube 

5x Tris-Glycine-SDS Loading Buffer – Mercaptoethanol (1 ml) ID1642 1 ml 5x Tris-Glycine-SDS Loading Buffer, Mercaptoethanol in a separate tube 

5x Tris-Glycine-SDS Loading Buffer (1 ml) ID1634 1 ml 5x Tris-Glycine-SDS Loading Buffer 

5x Tris-Glycine Loading Buffer (10 ml) ID1644 10 ml 5x Tris-Glycine Loading Buffer 

5x NA Loading Buffer (10 ml) ID1654 10 ml 5x NA Loading Buffer 

5x Oligipurification Loading Buffer (10 ml) ID1664 10 ml 5x Oligopurification Loading Buffer 

25x Western Transfer Buffer (125 ml) IDW07 125 ml 25x Western-Transfer-Buffer 


25

IDMax polyacrylamide gels have been available for 2 years from Nippon Genetics Europe and have gained acceptance in many laboratories over other precast 

gels on the market thanks to its easy handling and very good separation of complex protein mixtures. The PAA gels are polymerized in a plastic cassette. This 

cassette, compared to conventional cassettes, is extremely easy to open (click mechanism). The cassettes are available in two sizes, 10 x 10 cm and 10 x 8 cm, 

and therefore compatible with the most gel chambers on the market (A compatibility list can be found at www.nippongenetics.eu). You also have the choice 

between gels with 10 wells (up to 50 µl of sample volume) and 15 wells (up to 35 µl of sample volume). For the separation of very complex protein mixtures, the use 

of SDS inside the gel matrix is advantageous. The IDMax gels are the only ones on the market, which are offered with SDS inside the gel matrix. The IDMax 

pre cast gels are available as Tris-Glycine, Tris-Glycine-SDS, TAE and TBE gels at different percentages and as well as gradient gels (see table on the right side). 

Further information: 

๏ The gel matrix is slightly blue coloured in order to facilitate the sample loading. The wells are protected by an inserted plastic comb. 

๏ Inside the gel matrix is a unique number embedded, which remains visible even after drying the gel and allows the easy tracking of your results. 

๏ The TRIS-Glycine and TRIS-Glycine SDS gels include an optimized sample buffer. 

๏ The shelf life of the IDMax pre cast gels is 12 months, if stored at 4°C. 

Fig. 1: IDMax pre cast gels 

Would you like to take advantage of IDMax cassette for your own gels? 

We offer the patent-pending empty cartridges in two sizes (10x10 and 10x8 cm) and the corresponding combs 

separately. The highlight for your documentation: A running number is etched into the empty cartridge and will 

be transmitted to your gel and remains visible even after drying. 

Fig. 3: ID Cast gel cassettes 

Cat.No.: IDC001 (10 x 8 cm cassettes, 40 pcs.) 

Cat.No.: IDC002 (10 x 10 cm cassettes, 40 pcs.) 

IDMax PAA pre cast gels for proteins and nucleic acids 

Fig. 2: Embedded number allows easy documentation of 

your results 

ID Buffer solutions 

Running and sample buffers as ready solutions or as-measured 

powder in an aluminum pouch to make 1 liter of solution available. 

This eliminates the tedious weighing of SDS and other 

components. For ordering information please see page 25. 


IDMax PAA pre cast gels for proteins and nucleic acids 

Separation range 

Proteins (kDa) 

Native gels 

% / buffer system 

Ordering information IDMax TM PAA pre cast gels 

IDMax TM L/10well x% Tris-Native 

1.0 mm thick / 10 wells 

Large cassette (10x10 cm) 

Cat.No.: 2 gels / 10 gels 

IDMax TM S/10well x% Tris-Native 


Small cassette (10x8 cm) 


IDMax TM L/15well x% Tris-Native 




IDMax TM S/15well x% Tris-Native 




60-300 kDa 6% / Tris-Glycine S061 / IC061 S061s / IC061s S062 / IC062 S062s / IC062s 





30-400 kDa 4-12% / Tris-Glycine S211 / IC211 S211s / IC211s S212 / IC212 S212s / IC212s 





Proteins (kDa) 

Denaturing gels 


IDMax TM L/10well x% Tris-SDS 




IIDMax TM S/10well x% Tris-SDS 




IDMax TM L/15well x% Tris-SDS 




IDMax TM S/15well x% Tris-SDS 




60-300 kDa 6% / Tris-Glycine-SDS S361 / IC361 S361s / IC361s S362 / IC362 S362s / IC362s 





30-400 kDa 4-12% / Tris-Glycine-SDS S511 / IC511 S511s / IC511s S512 / IC512 S512s / IC512s 



5-150 kDa 10-20% Tris-Glycine-SDS S541 / IC541 S541s / IC541s S542 / IC542 S542s / IC542s 


nucleic acids (bp) 

TAE gels 


IDMax TM L/10well x% Tris-TAE 




IDMax TM S/10well x% TAE 




IDMax TM L/15well x% TAE 




IDMax TM S/15well x% TAE 




120-2500 bp 5% / TAE S651 / IC651 S651s / IC651s S652 / IC652 S652s / IC652s 



40-2500 bp 4-12% / TAE S811 / IC811 S811s / IC811s S812 / IC812 S812s / IC812s 

20-2000 bp 4-20% / TAE S821 / IC821 S821s / IC821s S822 / IC822 S822s / IC822s 


nucleic acids (bp) 

TBE Gele 


IIDMax TM L/10well x% Tris-TBE 




IIDMax TM S/10well x% Tris-TBE 




IDMax TM L/15well x% TBE 




IDMax TM S/15well x% TBE 




120-2500 bp 5% / TBE S851 / IC851 S851s / IC851s S852 / IC852 S852s / IC852s 



20-2000 bp 4-20% / TBE S1021 / IC1021 S1021s / IC1021s S1022 / IC1022 S1022s / IC1022s 


27

Bambanker TM cell freezing media for cultured cells 

It was never easier to freeze and preserve sensitive cell lines 

The freezing media Bambanker TM offered by the Japanese Genetech company Lymphotech was developed initially just for their own R&D projects. 

They needed a suitable medium for long-term storage of highly sensitive cell lines, such as lymphocytes. 

Thanks to the innovative formulation of the new freezing medium, an European Patent (EP 1347040) was granted and the development of this media 

was made commercially available. Today this innovative cell freezing media Bambanker TM is the market leader in Japan and characterized by many 

different published articles with very sensitive cell lines all over the world. 

European patent 

EP 1347040 

Flexible and user friendly: 

No dilution, no addition of components. 

We offer packs of: 

BB03 = 1 x 20 ml bottle 

BB02 = 5 x 20 ml bottles 

BB01 = 1 x 120 ml bottle 

Universal: 

All known cell lines can be stored for 12-24 months at -80°C or in liquid 

nitrogen. The rate of intact cells after thawing is improved significantly 

compared to traditional media, especially for sensitive cells. 

Serum-free: 

No risk of contamination and no interactions 

with serum proteins and your cells. 

Stable: 

Bambanker TM is stable if stored at 2-10°C for 

2 years. 


Fast: 

Gradual or programmable freezing is no longer necessary. Cells are 

spun down in the log growth phase and frozen in 1 ml Bambanker TM .

Why serum free media? 

The qualitative and quantitative composition of serum may be subject to strong fluctuations and each batch can react differently with specific cell types. 

In addition possible contamination from mycoplasma, viruses, prions or other viral particles can occur. Unidentified ingredients may interact with the 

cultured cells, which is especially the case if a very sensitive cell line such as embryonic stem cell is used. With the use of Bambanker TM all these 

barriersd are a thing of the past. 

Fig. 1: Cell freezing experiment with Ca9-22 (17 x 10 6 cells), a human gingival squamous carcinoma cell 

line. The left picture shows the cells before freezing with Bambanker TM and the right after freezing and thaw 

up. 

Fig. 3: Rate of intact cells after long term storage. Five different cell lines, preserved in three 

different media. Cell viability was determined after twelve months. 


Product Cat. No.: Content 

Bambanker TM (20 ml) BB03 20 ml freezing media Bambanker TM 

Bambanker TM (5 x 20 ml) BB02 5x 20 ml freezing media Bambanker TM 

Bambanker TM (120 ml) BB01 120 ml freezing media Bambanker TM 

Fig. 2: HSC cells (8,8 x 10 6 cells) 

Left side: Before freezing with Bambanker TM ; Right side: After freezing with Bambanker TM 

Bambanker Direct for Hybridoma cells 

No centrifugation step necessary 

Freezing media can be applied directly to the cell culture solution 

Especially suited for 96well plates - samples available! 


29

Mini Centrifuge Quiety 

The quietest centrifuge in your lab 

The Mini Centrifuge Quiety can handle up to 6 tubes (1.5 ml or 2.0 ml). Alternatively and with the use of the included adaptor two 8well strips (0,2 ml) can be 

centrifuged. The speed is 6000 rpm, which corresponds to 2000 xg. 

The adaptor cn be changed easily. The centrifuge can be used worldwide because 100 V - 240 V can be applied. 

The lid can‘t be open during centrifugation. The centrifuge must be turned off first. 

Finally this mini centrifuge is called Quiety because of very low noise, which is very convenient for your lab work- 



Homogenisator Mixy 

For convenient handling of your samples 

The Homogenisator Mixy facilitates the homogenization of your samples. Animal tissue as 

well as plant samples can be processed. Disposable pestles for the homogenization in 1.5 

ml tubes (Pestles 1.5 cm 3 ) or 0.5 ml tubes (Pestles 0.5 cm 3 ) can be ordered separately. 

The Homogenisator Mixy is operated with 4 Mignon AA batteries. Batteries and Pestles 

must be ordered separately. 


Parameter Mini Centrifuge Quiety 

Dimensions 

Weight (unloaded) 

Current 

Speed 

Adapter 

Mini Centrifuge Quiety NG001 Mini Centrifuge Quiety with adaptor for single tubes and 8well strips 



Homogenisator Mixy NG005 Homogenisator Mixy (without batteries) 

Pestles 0.5 cm 3 (100) NG007 100 Disposable Pestles 1.5 cm 3 

Pestles 1.5 cm 3 (100) NG006 100 Disposable Pestles 0.5 cm 3 


140 mm x 125 mm x 115 mm 

830 g 

100-240 V; 260 mA; 50/60 Hz 

6.000 rpm / 2.000 x g 

6 x 1.5/2.0 ml tubes 

2 x 0.2 ml 8well strips

• Please have a look at our WEB page (www. nippongenetics.eu) and/or to our Facebook page (Nippon Genetics Europe) in 

order to see all our new developments, customer testimonials and new application notes for existing products 

FastGene ® is a registered trademark of Nippon Genetics Europe GmbH, Dueren, Germany 

TaqMan ® is a registered trademark of Applied Biosystems, Carlsbad, USA 

Mastercycler ® is a registered trademark of Eppendorf; Hamburg, Germany 

Mupid TM iis a registered trademark of Advance, Tokyo, Japan 

Bambanker TM iis a registered trademark of Lymphotec, Tokyo, Japan 

IDMax TM is a registered trademark of DGel Sciences, Montreal, Kanada 

PCR is patented by Hoffmann-La Roche, Switzerland 

SYBR is a registered trademark of Life Technologies 

All products shown and described in this catalogue are for research use only 


Notes 

31

here - Nippon Genetics

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